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Effect of Same-dose Single or Dual Field Irradiation on Damage to Miniature Pig Parotid Glands
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作者 Xing Yan Bo Hai +3 位作者 Zhao-chen Shan Chang-yu Zheng Chun-mei Zhang Song-lin Wang 《International Journal of Oral Science》 SCIE CAS CSCD 2009年第1期16-25,共10页
Aim To evaluate the effect of single or dual field irra- diation (IR) with the same dose on damage to miniature pig parotid glands. Methodology Sixteen miniature pigs were divided into two IR groups (n=6) and a co... Aim To evaluate the effect of single or dual field irra- diation (IR) with the same dose on damage to miniature pig parotid glands. Methodology Sixteen miniature pigs were divided into two IR groups (n=6) and a control group (n=4). The irradi- ation groups were subjected to 20 Gy X-radiation to one parotid gland using single-field or dual-field modality by linear accelerator. The dose-volume distributions between two IR groups were compared. Saliva from parotid glands and blood were collected at 0, 4, 8 and 16 weeks after irradiation. Parotid glands were removed at 16 weeks to evaluate tissue morphology.Results The irradiation dose volume distributions were significantly different between single and dual field irradi- ation groups (t----4.177, P=0.002), although dose volume histogramin (DVH) indicated the equal maximal dose in parotid glands. Saliva flow rates from IR side decreased dramatically at all time points in IR groups, especially in dual field irradiation group. The radiation caused changes of white blood cell count in blood, lactate dehydrogenase and amylase in serum, calcium, potassium and amylase in saliva. Morphologically, more severe radiation damage was found in irradiated parotid glands from dual field irradi- ation group than that from single field irradiation group. Conclusion Data from this large animal model demons- trated that the radiation damage from the dual field irradiation was more severe than that of the single field irradiation at the same dose, suggesting that dose-volume distribution is an important factor in evaluation of the radiobiology of parotid glands. 展开更多
关键词 irradiation damage miniature pig parotidgland
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水通道基因治疗小型猪腮腺放射损伤的研究 被引量:7
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作者 单兆臣 李钧 +3 位作者 刘晓勇 张春梅 Baum BJ 王松灵 《北京口腔医学》 CAS 2005年第3期141-144,共4页
目的研究腺病毒介导水通道基因对小型猪腮腺放射损伤的治疗效果及其相应表达关系。方法19只实验用小型猪一侧腮腺给予20Gy放射剂量照射,照射后17周,转导不同浓度的水通道蛋白基因、荧光素酶基因和病毒缓冲液,分析唾液流率变化和目的基... 目的研究腺病毒介导水通道基因对小型猪腮腺放射损伤的治疗效果及其相应表达关系。方法19只实验用小型猪一侧腮腺给予20Gy放射剂量照射,照射后17周,转导不同浓度的水通道蛋白基因、荧光素酶基因和病毒缓冲液,分析唾液流率变化和目的基因表达。结果109pfu AdCMVhAQP1转导后3天和7天腮腺唾液流率明显增加,分别恢复到放射前腮腺唾液流率81±18%(P=0.024)和69±20%(P=0.058),14天下降到转导前水平,对照组均未见腮腺唾液流率明显变化。转导109pfu AdCMVhAQP1组免疫组织化学显示,AQP1主要表达在导管上皮,Western blotting分析有外源性水通道蛋白表达,而对照组未见AQP1表达。结论109pfu AdCMVhAQP1明显增加小型猪腮腺放射损伤唾液流率,转导的水通道基因具有潜在治疗涎腺放射损伤的临床应用价值。 展开更多
关键词 基因转导 放射损伤 小型猪 水通道蛋白
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