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Pathological unit and the octagonal en bloc resection of thoracic ossification ligamentum flavum
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作者 赵华健 《外科研究与新技术》 2011年第2期100-100,共1页
Objective To describe the pathological unit and octagonal en bloc resection for the treatment of ossification ligamentum flavum(OLF)in thoracic spine with spondylotic myelopathy.Methods Ninety-five patients from Janua... Objective To describe the pathological unit and octagonal en bloc resection for the treatment of ossification ligamentum flavum(OLF)in thoracic spine with spondylotic myelopathy.Methods Ninety-five patients from January 2002 to January 展开更多
关键词 OLF JOA Pathological unit and the octagonal en bloc resection of thoracic ossification ligamentum flavum
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Selection of surgical methods for thoracic ossification of ligamentum flavum combined with cervical spondylotic myelopathy
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作者 孙垂国 《外科研究与新技术》 2011年第2期82-82,共1页
Objective To investigate the difference between different surgical methods for thoracic ossification of ligamentum flavum(OLF) combined with cervical spondylotic myelopathy(CSM) . Methods From January 1991 to January ... Objective To investigate the difference between different surgical methods for thoracic ossification of ligamentum flavum(OLF) combined with cervical spondylotic myelopathy(CSM) . Methods From January 1991 to January 2003,56 cases 展开更多
关键词 OPLL Selection of surgical methods for thoracic ossification of ligamentum flavum combined with cervical spondylotic myelopathy CSM
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Diagnosis and one stage surgical treatment of lower thoracic ossification of ligamentum flavum combined with lumbar spinal stenosis
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作者 王哲 《外科研究与新技术》 2011年第2期95-96,共2页
Objective To study the diagnosis and treatment of lower thoracic ossification of ligamentum flavum(OLF) combined with lumbar spinal stenosis.Methods Retrospective analysis was carried out on 11 cases of lower thoracic... Objective To study the diagnosis and treatment of lower thoracic ossification of ligamentum flavum(OLF) combined with lumbar spinal stenosis.Methods Retrospective analysis was carried out on 11 cases of lower thoracic OLF combined with 展开更多
关键词 ODI OLF Diagnosis and one stage surgical treatment of lower thoracic ossification of ligamentum flavum combined with lumbar spinal stenosis
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Laminar thinned-segmented decompression for treatment of thoracic ossification of ligamentum flavum with myelopathy
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作者 郝定均 《外科研究与新技术》 2011年第2期103-103,共1页
Objective To evaluate the effects of the laminar thinned-segmented decompression technique in the treatment of thoracic ossification of ligamentum flavum with spondylotic myelopathy.Methods From January 1999 to Januar... Objective To evaluate the effects of the laminar thinned-segmented decompression technique in the treatment of thoracic ossification of ligamentum flavum with spondylotic myelopathy.Methods From January 1999 to January 2009,126 cases 展开更多
关键词 JOA Laminar thinned-segmented decompression for treatment of thoracic ossification of ligamentum flavum with myelopathy
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En bloc resection of semi-facet and lamina for thoracic ossification of ligamentum flavum with epidural adhesion
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作者 张志成 《外科研究与新技术》 2011年第2期101-102,共2页
Objective To explore the strategy and outcomes of surgical treatment of thoracic ossification of ligamentum flavum(OLF),especially combined with ossification of posterior longitudinal ligament,thoracic kyphosis and ep... Objective To explore the strategy and outcomes of surgical treatment of thoracic ossification of ligamentum flavum(OLF),especially combined with ossification of posterior longitudinal ligament,thoracic kyphosis and epidural 展开更多
关键词 OPLL En bloc resection of semi-facet and lamina for thoracic ossification of ligamentum flavum with epidural adhesion
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Surgical procedures of thoracic ossification of the ligamentum flavum
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作者 李方财 《外科研究与新技术》 2011年第2期102-103,共2页
Objective To investigate different surgical procedures for treatment of thoracic ossification of ligamentum flavum.Methods From January 1994 to June 2008,56 cases of thoracic ossification of ligamentum flavum underwent
关键词 Surgical procedures of thoracic ossification of the ligamentum flavum
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全可视下脊柱内镜技术治疗重度胸椎黄韧带骨化症 被引量:1
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作者 郑科 倪志豪 +5 位作者 董洲 祁家龙 韩国嵩 单涛 曾宪琦 王善松 《颈腰痛杂志》 2024年第2期268-270,共3页
目的探讨全可视下脊柱内镜技术治疗重度胸椎黄韧带骨化症的临床疗效分析。方法2018年1月~2022年12月,应用全可视脊柱内镜技术治疗例重度胸椎黄韧带骨化症患者共16例,男11例、女5例,年龄39~73岁、平均54.5岁;病程4~37个月、平均18.5个月... 目的探讨全可视下脊柱内镜技术治疗重度胸椎黄韧带骨化症的临床疗效分析。方法2018年1月~2022年12月,应用全可视脊柱内镜技术治疗例重度胸椎黄韧带骨化症患者共16例,男11例、女5例,年龄39~73岁、平均54.5岁;病程4~37个月、平均18.5个月,随访8~36个月。结果患者术前、术后1个月、6个月和末次随访时的胸背痛及下肢痛视觉模拟评分(visual analogue scale,VAS)分别为(7.06±0.85)分、(2.88±0.62)分、(1.81±0.54)分、(1.25±0.45)分,术后各时间段的VAS评分均显著低于术前,差异有统计学意义(P<0.01)。患者术前、3个月和6个月末次随访JOA评分分别为(12.43±1.26)分、(20.69±1.62)分、(23.62±1.20)分、(26.25±1.07)分,术后各时间段的JOA评分均显著优于术前,差异有统计学意义(P<0.01)。患者术前、术后3个月和6个月和末次随访时的Oswestry功能障碍指数(Oswestry disability index,ODI)分别为(68.9±3.3)%、(30.9±2.5)%、(19.0±3.0)%、(13.1±2.9)%。术后1个月、6个月及末次随访时均较术前明显改善(P<0.05)。结论全可视下脊柱内镜技术治疗重度胸椎黄韧带骨化症安全性高、术后恢复快、疗效明确。 展开更多
关键词 黄韧带骨化 脊柱内镜 胸椎
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Computer-assisted minimally invasive spine surgery for resection of ossification of the ligamentum flavum in the thoracic spine 被引量:12
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作者 Yuan Qiang Zheng Shan Tian Wei 《Chinese Medical Journal》 SCIE CAS CSCD 2014年第11期2043-2047,共5页
Background Ossification of the ligamentum flavum (OLF) has been widely recognized as one of the main causes of thoracic spinal canal stenosis and thoracic myelopathy.Decompression is the only effective strategy for ... Background Ossification of the ligamentum flavum (OLF) has been widely recognized as one of the main causes of thoracic spinal canal stenosis and thoracic myelopathy.Decompression is the only effective strategy for treating thoracic myelopathy caused by OLF.The purpose of this study was to describe the clinical outcomes of computer-assisted minimally invasive spine surgery (CAMISS) for posterior decompression in patients with thoracic myelopathy caused by OLF.Methods In all cases,the surgical procedure was performed with the assistance of an intraoperative three-dimensional navigation system.Decompression of the spinal cord was performed with a high-speed drill; the supraspinal ligaments and spinous process were partially preserved.The outcomes were evaluated by a modified Japanese Orthopedic Association (JOA) scoring system and recovery rates.Results The mean duration of follow-up for the 14 cases was 3.9 years.All patients experienced neurological recovery,the mean JOA score improving from 6.1 points preoperatively to 8.6 points at final follow-up and the mean rate of recovery being 52.7% (excellent in two cases,good in eight,fair in three,and unchanged in one).Conclusion CAMISS is a safe and effective procedure for resection of the OLF in the thoracic spine. 展开更多
关键词 computer-assisted minimally invasive spine surgery ligamentum flavum ossification thoracic spinal canal stenosis DECOMPRESSION
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Clinical results and intramedullary signal changes of posterior decompression with transforaminal interbody fusion for thoracic myelopathy caused by combined ossification of the posterior longitudinal ligament and ligamentum flavum 被引量:11
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作者 Wang Lin-feng Liu Fa-jing +3 位作者 Zhang Ying-ze Shen Yong Ding Wen-yuan Xu Jia-xin 《Chinese Medical Journal》 SCIE CAS CSCD 2013年第20期3822-3827,共6页
Background Surgical treatment of thoracic myelopathy caused by ossification of the posterior longitudinal ligament (OPLL) and ossification of the ligamentum flavum (OLF) is technically demanding, and the results t... Background Surgical treatment of thoracic myelopathy caused by ossification of the posterior longitudinal ligament (OPLL) and ossification of the ligamentum flavum (OLF) is technically demanding, and the results tend to be unfavorable. Various operative approaches and treatment strategies have been attempted, and posterior decompression with transforaminal thoracic interbody fusion (PTTIF) may be the optimal method with which the anterior-posterior compression was removed in one step. It is comparatively less traumatic with fewer serious complications. 展开更多
关键词 transforaminal thoracic interbody fusion thoracic myelopathy ossification of posterior longitudinal ligament ossification of ligamentum flavum intramedullary signal change
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Spinal Cord Kinking in Thoracic Myelopathy Caused by Ossification of the Ligamentum Flavum 被引量:2
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作者 Ting Wang Min Pan +4 位作者 Chu-Qiang Yin Xiu-Jun Zheng Ya-Nan Cong De-Chun Wang Shu-Zhong Li 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第19期2595-2598,共4页
Background:Ossification of the ligamentum flavum (OLF) is being increasingly recognized as a cause of thoracic myelopathy.This study was to describe a rare clinical entity of spinal cord kinking (SK) in thoracic ... Background:Ossification of the ligamentum flavum (OLF) is being increasingly recognized as a cause of thoracic myelopathy.This study was to describe a rare clinical entity of spinal cord kinking (SK) in thoracic myelopathy secondary to OLF.Methods:The data of 95 patients with thoracic myelopathy secondary to OLF were analyzed retrospectively.The incidence and location of SK were determined using preoperative magnetic resonance imaging (MRI).The clinical presentation and radiological characteristics in patients with SK were analyzed.Posterior en bloc laminectomy with OLF was performed,and the surgical results were evaluated.Results:SK was found in seven patients (7.4%) based on preoperative MRI.The patients included one male and six females with an average age of 55.6 years (range,48-64 years).Five patients presented with radiculomyelopathy and two presented with typical thoracic myelopathy of spastic paraparesis.In all cases,the kinking was located just above the end of the spinal cord where the conus medullaris (CM) was compressed by the OLF.The degree of SK varied from mild to severe.The tip of the CM was located between the upper third of T1 1 to the lower third of L 1,above the lower edge of L 1.With an average follow-up of 30.4 months,the modified Japanese Orthopedic Association score significantly improved from 5.7 ± 1.8 preoperatively to 8.9 ± 1.4 postoperatively (t =12.05;P < 0.0001) with an improvement rate of 63.1 ± 12.3%.Conclusions:SK is a rare radiological phenomenon.It is typically located at the thoracolumbar junction,where the CM is compressed by the OLF.Our findings indicate that these patients may benefit from a posterior decompressive procedure. 展开更多
关键词 ossification of ligamentum flavum Spinal Cord Kinking thoracic Myelopathy
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Thoracic cord compression caused by contiguous multilevel ossification of ligamentum flavum in Chinese patients 被引量:2
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作者 王凯 陈新 《Chinese Journal of Traumatology》 CAS 2007年第4期213-217,共5页
Objective: To explore the epidemiology, clinical presentation, radiology and surgical treatment outcome in Chinese patients with myelopathy caused by contiguous multilevel ossification of ligamentum flavum. Methods.... Objective: To explore the epidemiology, clinical presentation, radiology and surgical treatment outcome in Chinese patients with myelopathy caused by contiguous multilevel ossification of ligamentum flavum. Methods. Medical notes and imaging data of 18 Chinese patients (14 males and 4 females, aged 43-72 years, mean: 57 years ) with myelopathy caused by contiguous multilevel ossification of ligamentum flavum were studied retrospectively in this article. The diagnosis was based on clinical examination, X-ray films, computerized tomography (CT) and magnetic resonance imaging (MRI) scanning results and pathological results. Sixteen patients were treated by laminectomy and two by laminoplasty, The average follow-up duration was 34 months (range, 28-49 months ). The outcome was evaluated by Japanese Orthopaedics Association (JOA) Score. Results: The average time for occurring clinical symptoms was 7.5 months (range, 2 days-16 months). All the 18 cases presented with clinical evidences of chronic and progressive thoracic spinal cord compression, which included bilateral leg weakness, spastic gait, numbness in lower limbs, paresthesia in terminal and perineum, and urinary incontinence. Neurological examination revealed severe spastic paraparesis, absence of abdominal reflexes, and reduction of the sensory function below the compression level. The mean JOA score before operation was 3. 6 (range, 0-6 ). MRI and CT scans of the thoracic spine confirmed the presence of contiguous multilevel ossification of the ligamentum flavum. The mean recovery rate after surgery in terms of JOA score was 66.3 % ( range, 33.3 %- 100% ), with a mean final JOA score of 8. 3. Thoracic decompression laminectomy or laminoplasty could result in a good postoperative outcome. Conclusions : Contiguous multilevel ossification of the ligamentum flavum is not a common cause of myelopathy in Chinese population and should be treated as early as possible. MRI and CT scan examinations may diagnose the presence of thoracic ossification of ligamentum flavum (OLF). Posterior decompression, especially with en bloc dissection of the laminae, can obtain satisfactory results. 展开更多
关键词 ossification ligamentum flavum thoracic cord compression CHINESE
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Delta大通道脊柱内镜技术治疗胸椎黄韧带骨化症疗效分析
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作者 窦荣声 张羽 +4 位作者 于明东 闫浩 朱昱山 魏汝硕 王炳武 《实用骨科杂志》 2024年第8期686-690,共5页
目的探讨Delta大通道脊柱内镜下单侧入路双侧减压技术治疗胸椎黄韧带骨化症的临床疗效。方法回顾性分析2019年8月至2022年1月潍坊市人民医院采用Delta大通道脊柱内镜治疗的13例胸椎黄韧带骨化症患者,其中男6例,女7例;年龄55~69岁,平均(6... 目的探讨Delta大通道脊柱内镜下单侧入路双侧减压技术治疗胸椎黄韧带骨化症的临床疗效。方法回顾性分析2019年8月至2022年1月潍坊市人民医院采用Delta大通道脊柱内镜治疗的13例胸椎黄韧带骨化症患者,其中男6例,女7例;年龄55~69岁,平均(62.62±4.01)岁。记录患者基本信息及围手术期相关指标,并在术后1个月、6个月进行随访,记录术前术后改良日本骨科协会评分(modified Japanese orthopaedic association score,mJOA)、美国脊髓损伤协会(American spinal injury association,ASIA)感觉评分(ASIA sense score,ASS)和双下肢运动评分(ASIA motor score,AMS)变化情况。以mJOA评分计算术后6个月随访时的改善率(recover rate,RR)及优良率,对RR与性别、年龄、术前mJOA评分、影像学分型及病程进行相关性分析。结果平均手术时间为(108.46±16.12)min,平均出血量(23.46±7.47)mL,平均住院时间(6.08±0.86)d。术后随访时间为6~35个月,平均(16.77±9.33)个月,术后1个月及6个月随访时,mJOA、ASS、AMS评分均优于术前(P<0.05),平均RR为(55.69±19.73)%,优良率为69.23%。RR与术前病程呈负相关(P<0.001),与术前mJOA评分呈正相关(P=0.029),与性别、年龄、影像学分型均无明显相关性(P>0.05)。结论Delta大通道脊柱内镜技术治疗胸椎黄韧带骨化症安全可靠,具有术后恢复快、创伤小、出血量少等优势,短期效果良好。 展开更多
关键词 脊柱内镜 单侧入路双侧减压 胸椎黄韧带骨化 并发症
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Endo-ULBD治疗胸椎黄韧带骨化症的安全性与有效性研究
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作者 木热提·卡哈尔 地力木拉提·艾克热木 +1 位作者 张树文 徐阔 《实用骨科杂志》 2024年第8期691-695,700,共6页
目的评估经皮脊柱内镜单侧椎板切开双侧减压(percutaneous endoscopic unilateral laminectomy for bilateral decompression,Endo-ULBD)技术治疗胸椎黄韧带骨化症(thoracic ossification of ligamentum flavum,TOLF)的安全性与有效性... 目的评估经皮脊柱内镜单侧椎板切开双侧减压(percutaneous endoscopic unilateral laminectomy for bilateral decompression,Endo-ULBD)技术治疗胸椎黄韧带骨化症(thoracic ossification of ligamentum flavum,TOLF)的安全性与有效性。方法回顾性分析新疆维吾尔自治区人民医院2019年1月至2023年1月收治的28例TOLF患者,其中14例接受Endo-ULBD手术(Endo-ULBD组),其中男7例,女7例;年龄50~73岁,平均(61.79±8.33)岁。14例接受传统后路椎板切除或联合融合固定手术(开放组),其中男10例,女4例;年龄50~70岁;平均(60.57±6.10)岁。对比两组患者的一般基线资料以及手术时间、住院时间、术中出血量、术后疼痛视觉模拟评分(visual analogue scale,VAS)、日本骨科协会(Japanese orthopaedic association,JOA)评分、日常生活活动能力(activities of daily living,ADL)、Oswestry功能障碍指数(Oswestry disability index,ODI)及并发症情况。结果两组术中出血量及住院时间比较,差异有统计学意义(P<0.05),但手术时间上差异无统计学意义(P>0.05)。两组患者术后1、6个月以及末次随访中的VAS、JOA、ADL、ODI评分比较,差异无统计学意义(P>0.05),但两组组内各时间点比较,上述指标差异均有统计学意义(P<0.05)。Endo-ULBD组未出现脑脊液漏、硬膜外血肿、脊髓神经功能恶化、神经损伤或切口感染等并发症,而传统手术组有2例(14.2%)发生脑脊液漏,1例(7.1%)术后头痛、颈痛。结论Endo-ULBD是治疗TOLF的有效且安全的方法,适用于包括硬膜骨化在内的病例,且不影响胸椎稳定性。 展开更多
关键词 胸椎黄韧带骨化症 微创手术 经皮内镜单侧椎板切除术 手术减压
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环抱法切除胸椎黄韧带骨化症的临床疗效与解剖学基础
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作者 黄阳亮 钟祎 刘少喻 《中国临床解剖学杂志》 CSCD 北大核心 2023年第1期87-91,共5页
目的明确I期后入路徒手环抱法切除胸椎黄韧带骨化症的手术指征、临床疗效与解剖学基础。方法回顾2010年1月至2018年6月符合以下指征行I期后入路徒手环抱法手术切除胸椎骨化黄韧带患者20例(男9例,女11例,平均年龄55.2岁,43至75岁):第一... 目的明确I期后入路徒手环抱法切除胸椎黄韧带骨化症的手术指征、临床疗效与解剖学基础。方法回顾2010年1月至2018年6月符合以下指征行I期后入路徒手环抱法手术切除胸椎骨化黄韧带患者20例(男9例,女11例,平均年龄55.2岁,43至75岁):第一诊断为胸椎黄韧带骨化;排除Sato分型中的融合型及结节型;排除术前CT检查具有硬膜骨化征患者;排除各节段的单侧骨化物椎管占有率大于55%患者。收集术前与末次随访的改良JOA评分、临床解剖学特点及手术时间、出血量、并发症等临床资料,予以分析总结。结果20例患者均行环抱法经后路I期切除骨化韧带,共36个节段。平均手术时间116 min,出血量131 mL。术中有1例脑脊液漏,术中予缝合,术后无症状。病例均得以随访,随访时间48~54个月,平均50.9个月。术前mJOA评分(7.50±1.02),末次随访(10.25±0.62),差异有统计学意义(P<0.05)。术后改善率平均80.43%,优13例,良7例。未出现复发病例。结论对符合手术指征的黄韧带骨化患者行环抱法切除术具有操作简单,辅助工具少,手术时间短,效疗确切的优点。术后未见复发,预后良好,值得临床推广。 展开更多
关键词 胸椎 黄韧带骨化 手术指征 解剖学 后入路 徒手
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手术治疗胸椎黄韧带骨化症的疗效及其影响因素 被引量:58
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作者 陈仲强 孙垂国 +3 位作者 党耕町 刘忠军 郭昭庆 齐强 《中国脊柱脊髓杂志》 CAS CSCD 2006年第7期485-488,共4页
目的:探讨胸椎黄韧带骨化症手术治疗的疗效及其影响因素。方法:回顾性总结1986年1月至2003年4月我院采用“揭盖式”胸椎管后壁切除术治疗的135例胸椎黄韧带骨化症患者的资料,随访术后脊髓功能恢复情况,分析患者年龄、术前病程、手术节... 目的:探讨胸椎黄韧带骨化症手术治疗的疗效及其影响因素。方法:回顾性总结1986年1月至2003年4月我院采用“揭盖式”胸椎管后壁切除术治疗的135例胸椎黄韧带骨化症患者的资料,随访术后脊髓功能恢复情况,分析患者年龄、术前病程、手术节段与部位、影像学分型及JOA评分等与疗效的关系。结果:135例中82例获得随访,随访率60.7%,平均随访时间5年6个月(2~14年),术后优良率74.4%,有效率92.7%;68.4%的患者在术后2年内恢复停滞,26.3%的患者主诉术后2 ̄5年仍有缓慢恢复;患者的术前病程、年龄、手术节段对术后疗效有显著影响(P<0.05);手术节段累及胸腰段者术后疗效较局限于中上胸椎者差。结论:“揭盖式”椎管后壁切除术是治疗胸椎黄韧带骨化症可靠、有效的手术方式。患者术前病程、年龄及手术部位是影响手术疗效的主要因素。 展开更多
关键词 胸椎 黄韧带骨化 治疗 预后
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胸椎黄韧带骨化的流行病学调查——993例以胸部症状来诊患者的CT观察 被引量:23
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作者 郎宁 袁慧书 +5 位作者 王宏磊 廖晶 李曼 郭福新 史珊 陈仲强 《中国脊柱脊髓杂志》 CAS CSCD 北大核心 2011年第9期764-768,共5页
目的:研究胸椎黄韧带骨化(ossification of the ligamentum flavum,OLF)发生年龄、节段分布、形态变化等的流行病学特点。方法:回顾性分析2010年2月以胸部症状来我院就诊的993例患者(男506,女487,年龄5~102岁,平均60岁)的胸部多... 目的:研究胸椎黄韧带骨化(ossification of the ligamentum flavum,OLF)发生年龄、节段分布、形态变化等的流行病学特点。方法:回顾性分析2010年2月以胸部症状来我院就诊的993例患者(男506,女487,年龄5~102岁,平均60岁)的胸部多排探测CT(multidetector computed tomography,MDCT)片、轴位片结合矢状位CT片,分析胸椎黄韧带骨化的患病率及发生的节段、厚度、位置、硬膜囊压迫等情况,观察性别、年龄及发生节段等与OLF的关系。患病率采用《北京市2008年人口年龄构成统计》进行人群标化。结果:总体标化患病率为63.9%;男性标化患病率(68.5%)高于女性(59.0%);50~59岁年龄组的患病率最高(79.2%),但10~19岁人群也有OLF发生,其患病率为50.0%;T10~T11(44.0%)和T11~T12(41.6%)节段OLF的患病率亦较高。结论:被调查人群中,胸椎OLF患病率较高,出现年龄较早,发生于下胸段多于中上胸段,患病率与年龄具有一定的相关性。 展开更多
关键词 骨化 黄韧带 胸椎 流行病学
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胸椎黄韧带骨化症 被引量:13
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作者 刘立成 张佐伦 +4 位作者 于锡欣 路小勇 袁泽农 孙建民 王峰 《中国矫形外科杂志》 CAS CSCD 2002年第1期5-7,共3页
目的 :研究胸椎黄韧带骨化症 (OLF)的诊断与手术治疗方法 ,探讨其病因、病理、手术效果与并发症。方法 :采用回顾性研究方法对手术治疗的 46例OLF病例进行分析。手术方法均采用椎管后壁切除术。结果 :术后 40例随访 1.5年以上 ,6例经短... 目的 :研究胸椎黄韧带骨化症 (OLF)的诊断与手术治疗方法 ,探讨其病因、病理、手术效果与并发症。方法 :采用回顾性研究方法对手术治疗的 46例OLF病例进行分析。手术方法均采用椎管后壁切除术。结果 :术后 40例随访 1.5年以上 ,6例经短期观察。优 2 0例 ,良 17例 ,可 5例 ,差 4例。结论 :OLF所致的脊髓病表现复杂 ,手术治疗必须慎重操作。手术近期效果良好。以“孤立—磨薄—分割—切除”的方法行椎管后壁切除减压是治疗本病安全。 展开更多
关键词 胸椎黄韧带骨化症 OLF 诊断 手术治疗 病因 临床表现
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蛇床子素对体外培养成骨细胞增殖与分化成熟的影响 被引量:28
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作者 明磊国 葛宝丰 +4 位作者 陈克明 马慧萍 翟远坤 周建 李志锋 《中国骨伤》 CAS 2010年第9期688-691,共4页
目的:研究蛇床子素对体外培养大鼠成骨细胞(rat calvarial osteoblasts,ROB)增殖与分化成熟的影响。方法:取新生SD大鼠颅骨,多次酶消化法获得成骨细胞,培养于含10%FBS的MEM培养液中,3d后首次换液,铺满90%皿底后传代培养。增殖分析采用9... 目的:研究蛇床子素对体外培养大鼠成骨细胞(rat calvarial osteoblasts,ROB)增殖与分化成熟的影响。方法:取新生SD大鼠颅骨,多次酶消化法获得成骨细胞,培养于含10%FBS的MEM培养液中,3d后首次换液,铺满90%皿底后传代培养。增殖分析采用96孔板MTT法分析,加入培养液中终浓度分别为1×10-4、1×10-5、1×10-6、1×10-7mol/L的蛇床子素;分化分析采用24孔板,于成骨性诱导培养第3、6、9、12、15天分别测碱性磷酸酶(alkaline phosphatase,ALP)活性,第8天用免疫组织化学法观察Ⅰ型胶原合成情况,第12天进行ALP组织化学染色,第14天进行茜素红染色和钙化结节计数。结果:终浓度为1×10-4mol/L蛇床子素对细胞增殖有抑制作用,而1×10-5mol/L浓度虽对ROB增殖无明显影响,但能显著提高ALP活性,促进Ⅰ型胶原表达,并增加钙化结节数量。结论:1×10-5mol/L蛇床子素能促进ROB分化成熟,应为中药蛇床子抗骨质疏松的有效成分。 展开更多
关键词 成骨细胞 细胞培养技术 蛇床子素 骨质疏松
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胸椎黄韧带骨化症的外科治疗及疗效影响因素分析 被引量:7
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作者 周健 陈农 +5 位作者 董健 李熙雷 周晓岗 方涛林 林红 马易群 《复旦学报(医学版)》 CAS CSCD 北大核心 2010年第4期379-383,共5页
目的探讨胸椎黄韧带骨化症(ossification of ligamentum flavum,OLF)的手术治疗方法及其术后疗效的影响因素。方法回顾性分析2001年5月至2008年9月在我院进行手术治疗的39例胸椎OLF患者,其中30例采用"揭盖"式全椎板切除减压,9例采... 目的探讨胸椎黄韧带骨化症(ossification of ligamentum flavum,OLF)的手术治疗方法及其术后疗效的影响因素。方法回顾性分析2001年5月至2008年9月在我院进行手术治疗的39例胸椎OLF患者,其中30例采用"揭盖"式全椎板切除减压,9例采用改良后路全椎板切除减压及短节段椎弓根螺钉内固定治疗。随访术后脊髓功能恢复情况,分析术前病程、术前JOA评分(日本矫形外科学会,11分)、硬化节段、CT分型、脊髓信号改变与术后疗效的关系。结果39例患者均获得随访,随访时间在10个月~8年,平均54个月。根据JOA评分标准进行术前和术后的疗效评价。术后疗效评价为优19例、良14例、可6例,优良率达84.6%。术前病程短,JOA评分高,单节段骨化,CT分型为外侧型及矢状位T2加权像髓内信号无改变的病例术后疗效好。结论"揭盖"式全椎板切除减压和改良后路全椎板切除减压是治疗胸椎OLF可靠、有效的手术方式。患者术前病程、术前JOA评分、硬化节段、CT分型及压迫节段MRI示脊髓信号有无改变是影响术后疗效的主要因素。 展开更多
关键词 胸椎 黄韧带骨化 手术治疗 术后疗效 影响因素
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胸椎管狭窄症术后脑脊液漏继发皮下积液的治疗 被引量:8
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作者 王永强 刘晓光 +6 位作者 姜亮 韦峰 于淼 吴奉梁 党礌 周华 刘忠军 《北京大学学报(医学版)》 CAS CSCD 北大核心 2018年第4期657-661,共5页
目的:探讨胸椎管狭窄症术后脑脊液漏继发皮下积液的治疗方法。方法:回顾分析2005年1月至2014年12月北京大学第三医院骨科脊柱组完成手术的胸椎管狭窄症患者,其中术中、术后发生脑脊液漏者186例,选取其中继发皮下积液且规律随访的11例患... 目的:探讨胸椎管狭窄症术后脑脊液漏继发皮下积液的治疗方法。方法:回顾分析2005年1月至2014年12月北京大学第三医院骨科脊柱组完成手术的胸椎管狭窄症患者,其中术中、术后发生脑脊液漏者186例,选取其中继发皮下积液且规律随访的11例患者作为研究对象,治疗方法依据患者脑脊液漏严重程度以及胸脊髓病恢复情况决定。采取日本骨科协会(Japanese Orthopedic Association,JOA)改良11分评分评价患者术前及术后的脊髓功能,将手术前后的JOA评分进行统计学分析。结果:11例患者均获得随访,其中后纵韧带骨化合并黄韧带骨化者6例,均接受椎管后壁切除及"涵洞塌陷法"360°脊髓环形减压术,单纯黄韧带骨化行椎管后壁切除术者5例。随访时间30~131个月,平均(85±34)个月。术前症状持续时间3个月至8年,中位数18个月;术后引流管拔出时间2~6 d,平均(4.2±1.1)d;围手术期出现发热患者10例,最高体温(37.3~39.7)℃,其中高热患者2例,延长抗生素使用时间。10例患者采取保守治疗,随访发现脑脊液漏全部吸收,其中拔出引流管后加压包扎伤口者8例,局部穿刺抽液后加压包扎伤口者2例;1例患者因保守治疗无效,继发脑脊液假性囊肿而接受再次手术治疗。11例患者的JOA评分从术前的(3.8±1.6)分升高到末次随访时的(8.9±1.2)分,神经功能改善率为70.8%。没有患者出现切口感染、切口不愈合及颅内感染等并发症。结论:胸椎管狭窄症术后脑脊液漏继发皮下积液者多数可以采取保守治疗,只有出现脑脊液假性囊肿压迫脊髓时需要采取手术治疗。 展开更多
关键词 后纵韧带骨化 黄韧带骨化 胸椎 脑脊液漏 皮下积液
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