Numerical investigation on seismic performance of a shallow buried underground structure with isolation devices

A design procedure for improving the seismic performance of unequal-span underground structures by installing isolation devices at the top end of columns is proposed based on the seismic failure mode of frame-type underground structures and the design concept of critical support columns.A two-dimensional finite element model(FEM)for a soil-underground structure with an unequal-span interaction system was established to shed light on the effects of a complex subway station with elastic sliding bearings(ESB)and lead rubber bearings(LRB)on seismic mitigation.It was found that the stiffness and internal force distribution of the underground structure changed remarkably with the installation of isolation devices at the top end of the columns.The constraints of the beam-column joints were significantly weakened,resulting in a decrease in the overall lateral stiffness and an increase in the structural lateral displacement.The introduction of the isolation device effectively reduces the internal force and seismic damage of the frame column;however,the tensile damage to the isolation structure,such as the roof,bottom plate,and sidewall,significantly increased compared to those of the non-isolation structure.Although the relative slip of the ESB remains within a controllable range under strong earthquake excitation as well as frame columns with stable vertical support and self-restoration functions,the LRB shows a better performance during seismic failure and better lateral displacement response of the unequal-span underground structure.The analysis results provide new ideas and references for promoting the application of seismic isolation technology in underground structures.

Seismic performance of cable-sliding friction bearing system for isolated bridges

During past strong earthquakes, highway bridges have sustained severe damage or even collapse due to excessive displacements and/or very large lateral forces. For commonly used isolation bearings with a pure friction sliding surface, seismic forces may be reduced but displacements are often unconstrained. In this paper, an alternative seismic bearing system, called the cable-sliding friction bearing system, is developed by integrating seismic isolation devices with displacement restrainers consisting of cables attached to the upper and lower plates of the bearing. Restoring forces are provided to limit the displacements of the sliding component. Design parameters including the length and stiffness of the cables, friction coefficient, strength of the shear bolt in a fixed-type bearing, and movements under earthquake excitations are discussed. Laboratory testing of a prototype bearing subjected to vertical loads and quasi-static cyclic lateral loads, and corresponding numerical finite element simulation analysis, were carried out. It is shown that the numerical simulation shows good agreement with the experimental force-displacement hysteretic response, indicating the viability of the new bearing system. In addition, practical application of this bearing system to a multi-span bridge in China and its design advantages are discussed.

Isolation and cultivation of porcine hepatocytes for extracorporeal artificial liver support system

Objective To develop procedures for the successful harvesting of large quantities of viable and functional pig liver cells from abattoir organs.Methods The procedure included partial liver lobe retrograde perfusion and mechanical/enzymatic digestion of the liver tissue, followed by separation of the hepatocytes, based on size and density, from contaminating cell types.Results Digestion of the partial liver lobe resulted in an average yield of 1.39×109 cells (9.9×106 cells/g liver) with an average viability of 92.5%. The yield and viability of cells were improved by dispase/collagenase resultant digestion. The emergence of blebby cells was blocked by supplying oxygen to the cell isolation buffers. Isolated hepatocytes seeded onto polystyrene surfaces remained viable and functional at a level comparable to that of rat hepatocytes, although their function decreased over time.Conclusions Adult pig hepatocytes can be harvested with high yields and retain viability and differentiated function using this method. Abattoir pig livers can be an excellent source of hepatocytes for use as the biological component of artificial liver assist devices.