It was isolated eleven species of weevils family (Curculionidae) back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memo...It was isolated eleven species of weevils family (Curculionidae) back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memosae, Tanymecus sp., Cleonus orratus, Larinus maculosus, Lixus sp., Hypera sp., Amnoeleonus hiroglyphicus, Bangasternus planifrons, Phacephorus nubeculosus, Coniocleonus puseudooldiquus, Tanymecus insipidus.展开更多
[Objective] This study aimed to investigate the genetic variation of porcine circovirus type 2 (PCV2) in China. [Method] The strain was isolated from infected samples by cel passage and preliminarily identified by P...[Objective] This study aimed to investigate the genetic variation of porcine circovirus type 2 (PCV2) in China. [Method] The strain was isolated from infected samples by cel passage and preliminarily identified by PCR and IFA. Ful-length genome of the isolated strain was obtained by specific amplification for homology and phylogenetic analysis. [Result] A PCV2 strain was successful y isolated and named 201105ZJ, which could proliferate in PK15 cel lines. Specific fragments could be amplified by specific PCR assay. According to results of IFA assay, specif-ic immunofluorescence was observed; the TCID50 was low (102.67); the ful-length genome sequence of the isolated strain was 1 768 bp, sharing 94.1%-96.8% ho-mology with 13 reference strains; to be specific, the isolated strain exhibited the highest homology of 96.8% with AF055392PCV2a; the isolated strain 201105ZJ and reference strain AF055392 belonged to genotype PCV2a, exhibiting a distant genetic relationship with genotype PCV2c. [Conclusion] Characteristics of genetic variation of PCV2 isolate 201105ZJ provided theoretical basis for vaccine development, investi-gation of PCV2 pathogenesis, and prevention and control of porcine circovirus-as-sociated diseases (PCVAD) in East China.展开更多
To reduce the quadratic scaling of the series resistance(R)and sheet resistance(Rs)of the devices,physi-cal isolation of the large area devices into small pieces has been proven to be a reliable and cost-efficient pat...To reduce the quadratic scaling of the series resistance(R)and sheet resistance(Rs)of the devices,physi-cal isolation of the large area devices into small pieces has been proven to be a reliable and cost-efficient patterning technique.In this paper,we got an interesting result that the physical isolation did not show obvious effct on the photovoltaic performance of perovskite solar cells(PSCs)when fixing the active area.Three different isolation types,unetched ITO,etched ITO,and laser etching whole devices,have been induced to investigate the physical isolation roles.The results show that the electrons and holes could be collected efficiently in active area for all the isolation types.The proposed mechanism illustrates that the nonradiative recombination and recombination of electrons and hole in inactive area do not influence the performance of devices.This work may open a new way for the commer-cialization of PSCs by reducing the complex process and the etching costs.展开更多
文摘It was isolated eleven species of weevils family (Curculionidae) back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memosae, Tanymecus sp., Cleonus orratus, Larinus maculosus, Lixus sp., Hypera sp., Amnoeleonus hiroglyphicus, Bangasternus planifrons, Phacephorus nubeculosus, Coniocleonus puseudooldiquus, Tanymecus insipidus.
基金Supported by National Natural Science Foundation of China(31302071)Special Fund for Agro-scientific Research in the Public Interest(201303046)+1 种基金Independent Innovation Project of Jiangsu Province[CX(13)3065]Project of the Fourth Period of "333" High-level Personnel Training Program of Jiangsu Province(BRA2012194)~~
文摘[Objective] This study aimed to investigate the genetic variation of porcine circovirus type 2 (PCV2) in China. [Method] The strain was isolated from infected samples by cel passage and preliminarily identified by PCR and IFA. Ful-length genome of the isolated strain was obtained by specific amplification for homology and phylogenetic analysis. [Result] A PCV2 strain was successful y isolated and named 201105ZJ, which could proliferate in PK15 cel lines. Specific fragments could be amplified by specific PCR assay. According to results of IFA assay, specif-ic immunofluorescence was observed; the TCID50 was low (102.67); the ful-length genome sequence of the isolated strain was 1 768 bp, sharing 94.1%-96.8% ho-mology with 13 reference strains; to be specific, the isolated strain exhibited the highest homology of 96.8% with AF055392PCV2a; the isolated strain 201105ZJ and reference strain AF055392 belonged to genotype PCV2a, exhibiting a distant genetic relationship with genotype PCV2c. [Conclusion] Characteristics of genetic variation of PCV2 isolate 201105ZJ provided theoretical basis for vaccine development, investi-gation of PCV2 pathogenesis, and prevention and control of porcine circovirus-as-sociated diseases (PCVAD) in East China.
基金Supported by the National Natural Science Foundation of China(Nos.51772039,21703027,21902021,51972293)the Fun-damental Research Funds for the Central Universities,China[No.DUT16RC(4)77]the Grant-in-Aid for Scientific Research(KAK ENHI)Program,Japan(B,Grant No.19H02818).
文摘To reduce the quadratic scaling of the series resistance(R)and sheet resistance(Rs)of the devices,physi-cal isolation of the large area devices into small pieces has been proven to be a reliable and cost-efficient patterning technique.In this paper,we got an interesting result that the physical isolation did not show obvious effct on the photovoltaic performance of perovskite solar cells(PSCs)when fixing the active area.Three different isolation types,unetched ITO,etched ITO,and laser etching whole devices,have been induced to investigate the physical isolation roles.The results show that the electrons and holes could be collected efficiently in active area for all the isolation types.The proposed mechanism illustrates that the nonradiative recombination and recombination of electrons and hole in inactive area do not influence the performance of devices.This work may open a new way for the commer-cialization of PSCs by reducing the complex process and the etching costs.