Isoline致小鼠急性肝损伤后肝亲水性蛋白的双向电泳分析

目的:利用双向电泳技术(2-DE)观察吡咯里西啶生物碱isoline致小鼠急性肝损伤后对肝内亲水性蛋白表达的影响。方法:小鼠一次性灌胃给药isoline,通过血清生化指标检测和病理切片观察isoline的急性肝毒性,抽提肝组织亲水性蛋白做双向电泳分析,利用PDQuest软件以正常组作为参考胶进行匹配分析。结果:丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)检测和肝组织病理切片结果表明,口服isoline造成小鼠急性肝损伤,双向电泳图像分析发现与对照组相比,isoline组小鼠肝组织亲水性蛋白表达图谱中有39个蛋白斑点变化差异显著,其中新出现5个蛋白斑点,缺失10个蛋白斑点。结论:Isoline灌胃可以造成小鼠急性肝损伤,其中蛋白双向电泳分析结果中差异表达的蛋白质点可能参与了isoline的急性肝毒性作用。

Isoline两个新代谢物的制备及其理化性质(英文)

目的:为进一步研究isoline的代谢和毒理,采用化学方法制备其代谢产物。方法:分别采用水解,氮氧化和脱氢化反应将isoline转化为与代谢和毒理研究相关类型的产物。结果与结论:成功地制备了五个化合物,其中两个为新化合物,其结构经UV,IR,MS和NMR分析得以鉴定。

Two New Lactones Metabolized from Isoline by Rat Liver Microsomes

Two new metabolites, namely bisline lactone and isolinecic acid lactone, were isolated from the resultant incubates after a scale-up incubation of isoline with rat liver microsomes. Their structures were determined by spectroscopic data, especially those from 1D and 2D NMR experiments.

Generation methodology of isolines of earthquake ground motion

Due to limited financial resources and challenging geographical conditions, the number of seismic observation networks in China is still very small and they are not widely distributed. Therefore, the available earthquake records obtained after an earthquake have been limited. In this paper, auto-generation methods to obtain strong motion isolines under different conditions are proposed. To verify the accuracy of these methods, some examples, including application to the 2008 Wenchuan earthquake, are given.

Gossypium herbaceum Negative Mutant for Fiber Elongation a Useful Isoline for Identification of Genes for Fiber Elongation

Actin cytoskeleton plays an important role in cell morphogenesis in plants as demonstrated by pharmacological,biochemical,and genetic studies.The actin cytoskeleton may be involved in

Responses of Flowering Time to Elevated Carbon Dioxide among Soybean Photoperiod Isolines

Changes in the phenology of flowering in soybeans caused by long-term growth at elevated CO2 may be important to the responses of seed yield to elevated CO2. Here we utilized near-isogenic lines of soybeans differing in three genes influencing photoperiod sensitivity to determine whether these genes affected the response of flowering time to elevated CO2. Six isolines of Harosoy 63 were grown at ambient (380 μmol?mol-1) and elevated (560 μmol?mol-1) CO2 concentrations in the field using free-air CO2 enrichment systems, in air-conditioned glasshouses with natural summer photoperiods, and in indoor chambers with day lengths of 11, 13, 15, and 17 hours. The effect of CO2 concentration on flowering time varied with genotype, and there was also an interaction between CO2 and photoperiod in all genotypes, as indicated by ANOVA. Elevated CO2 accelerated flowering in some cases, and delayed it in other cases. For all three of the isolines with single dominant genes, elevated CO2 decreased the days to first open flower at the longest photoperiod. At the shortest photoperiod, elevated CO2 delayed flowering in all but one isoline. The all-recessive isoline had slower flowering at elevated CO2 at both the shortest and the longest photoperiods, and also in the field and in the glasshouse. Delayed flowering at elevated CO2 in the field and glasshouse was associated with an increased final number of main stem nodes. It is concluded that the E1, E3, and E4 genes each influenced how the time to first flowering was affected by CO2 concentration at long photoperiods.

Spatio-temporal Changes of ≥10℃ Accumulated Temperature in Northeastern China since 1961

The objective of this study was to provide reliable basis for decision making for national food security and layout and structure adjustment of grain production in the northeastern China. The data of mean daily air temperature of 1961-2009 from 106 meteorological stations in the northeastern China were chosen in this study. Using statistical methods and isoline method, the spatio-temporal changes of various decadal ≥10℃accumulated temperature and the climatic means of ≥10℃ accumulated temperature were studied in this paper. The results showed that 1） The geo- graphical distribution of ≥10℃ accumulated temperature in the northeastern China could be influenced directly by the latitude, longitude and altitude. If latitude moved one degree northward, the average decrease amplitude of the climatic means was 101.9℃ in the study area. 2） The means of decadal ≥10℃ accumulated temperature rose since the 1980s, and their increase amplitudes became larger in the 1990s and the 2010s obviously. Compared with those of the 1980s, ≥10℃ accumulated temperature increased by about 100℃ in the mountainous and plain areas in the 1990s; compared with those of the 1990s, ≥10℃ accumulated temperature increased by about 200℃ in the Hulun Buir High Plain and the Songnen Plain, and 100℃ in the Sanjiang Plain and the Liaohe Plain in the 2010s. 3） The means of the decada ≥10℃accumulated temperature for 106 meteorological stations in the northeastern China increased with the rate of 145.57℃/10yr in 1961-2009.4） The climatic means of ≥10℃ accumulated temperature increased from 1961-1990 to 1971-2000 and 1981-2009. Compared with the climatic mean of 1971-2000, that of 1981-2009 had increased by above 50℃ in most of the study area, even up to 156℃. Compared with the climatic mean of 1961-1990, that of 1981-2009 increased by above 100℃ in most parts of the study area, even up to 200℃. 5） The maximum northward shift, eastward and westward extension amplitudes of3100℃, 3300℃ and 3500℃ isolines were larger among all isolines for the climatic means of the three phases. Compared with the positions of the isolines of 1961-1990, those amplitudes of 31000C isoline of 1981-2009 were 145 km, 109 km and 64 km, respectively; those of 3300℃ isoline were 154 km, 54 km and 64 km, respectively; and the maximum northward shift of 3500℃ isoline was about 100 km.

The Electrical Resistivity of Cement Paste Incorporated with Retarder

A non contacting electrical resistivity measurement device was used for measuring the electrical resistivity of cement paste incorporated with retarder.The hydration process was divided into dissolving period,setting period,hardening period.With the increase of the retarder,the dissolution time will increase.The setting time happens between minimum point time and inflexion point time based on the electrical resistivity curve.The strength isoline was proposed to predict the strength trend.

Combined Action of Uniform Flow and Oscillating Flow Around Marine Riser at Low Keulegan-Carpenter Number

With the increase of petroleum and gas production in deep ocean, marine risers of circular cylinder shape are widely used in the offshore oil and gas platform. In order to research the hydrodynamic performance of marine risers, the dynamic mesh technique and User-Defined Function(UDF) are used to simulate the circular cylinder motion. The motion of a transversely oscillating circular cylinder in combination of uniform flow and oscillating flow is simulated. The uniform flow and oscillating flow both are in x direction. SIMPLE algorithm is used to solve the Navier-Stokes equations. The User-Defined Function is used to control the cylinder transverse vibration and the inlet flow. The lift and drag coefficient changing with time and the map of vorticity isolines at different phase angle are obtained. Force time histories are shown for uniform flow at Reynolds number(Re) of 200 and for the combination of uniform and oscillating flows. With the increase of amplitude of oscillating flow in combined flow, the change of lift amplitude is not sensitive to the the change of cylinder oscillating frequency. Lift amplitude increases with the increase of oscillating flow amplitude in the combined flow, but there is no definite periodicity of the lift coefficient. The drag and inertia force coefficients change when the maximum velocity of the oscillating flow increases in the combined flow. The vortex shedding near the circular cylinder shows different characteristics.

The Study of Two Geomagnetic Jerks in China

This paper analyzes the first differences of the annual means (annual rate) for the Y and Z components of the geomagnetic field from nine magnetic observatories in China, measured from 1985 to 2003. The 1991 jerk was obvious in the Y component measured but not clear for the Z component. Rapid changes in the Z components were ubiquitous around 2000-2001, but not seen for the Y component. External effects were removed from the monthly means by comparing the monthly mean of the geomagnetic field components at the observatories with the monthly time series of the A_p geomagnetic index. However, some examples were analyzed and showed whether external effects were removed or not, there was no marked distinction in determining the jerks in China for the Y component and the Z component of the geomagnetic field. Finally, the isolines of the first differences of the annual means were used to analyze the spatial and temporal distributions of the jerks.

Study of Electromagnetism Compatibility of Bleeper Station

Transmitted power will decrease, cross-modulate distortion and inter-modulate distortion will be caused and antenna pattern will shift if transmitted antennas are fixed together. All these will lead to the alteration of area coverage. According to the basic theory of electromagnetism compatibility a computer model is established. We do some quantitative analysis of the problems above and give total number, arrangement mode and distance between antennas of bleeper station that operate at 150 MHz. The relation of these factors above are also given. All these are basis of the arrangement of antenna group of bleeper station.

Predicting coal mining faults using combined rock relationships

By studying different compressive strengths and changes in the characteristics of rocks,five variables were selected to predict faults in coal mines. Drillholes in the mined area were divided into two populations, i.e., drillholes containing faults and drillholes without faults. Discriminant functions were established from the values of the five variables using Fisher's approach. Drillholes in the non-mined areas were allocated to one of the two populations by using discriminant functions. The terrenes of each drillhole were divided into 10 sections, above and below a minable coal seam. Each section has 10 layers of rocks. The population to which each drillhole in a section belongs is sorted out and the probability of each drillhole with faults obtained,i.e., a contour map of predicting the probability of faults in coal mining is shown. A comparison with the real distribution of faults shows that the precision of accurately predicting faults is greater than 70 per cent.

Analysis of Spatial Distribution Pattern of Tobacco Mosaic Virus (TMV) Based on Geostatistics

Using geostatistical method, the semi-variation function models of tobacco mosaic virus （TMV） in east-west and north-south directions were established, and the distribution pattern of TMV in large scale space was studied. The results showed that the distribution pattern of the disease in east-west and north-south directions belonged to linear model with abutment, and the spatial distribution pattern within the investigated areas was aggregated model. The spatial correlation distances in east-west and north-south directions were 29.953 4 and 47.813 8 km, and the spatial variabilities were 95.71% and 80.05%, respectively. This indicated that they had strong spatial correlation. Isoline map accessed by Kringing interpolation method could clearly reflect the spatial aggregated model.

Research on electromagnetic visualization method

It is significantly important to have the knowledge of electromagnet situation of battlefield,because it contains a lot of useful information to understand the situation of the whole battlefield.Electromagnet situation visualization would be very useful to help people to understand the situation,and handle battlefield situation,such as the size and quality of the electromagnetic radiation source,target objects,and operational purposes by the two parties engaged in combat scale.This paper proposes two electromagnet situation visualization methods,one is an isoline tracking algorithm based on floating rectangle and the other is an isoline filling algorithm based on linear interpolation,which also solves the problem of low accuracy and low efficiency.This paper also implements and displays the visualization results using different ways of demonstration modules,which show that the visualization results are more intuitive.

SOME CRITERIA OF THE EXISTENCE OF LIMIT CYCLES FOR PLANAR AUTONOMOUS SYSTEMS

The purpose of this paper is to give some necessary and sufficient conditionsfor the non intersection of trajectories of the planar autonomous system=P(x,y), =Q(x,y) (E) with the vertical isocline, and to present some results which guarantee the nonexistence and the existence of limit cycles of (E). our results extend and improve the corresponding ones in are extended and improved.