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Research on Multi-Core Processor Analysis for WCET Estimation
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作者 LUO Haoran HU Shuisong +2 位作者 WANG Wenyong TANG Yuke ZHOU Junwei 《ZTE Communications》 2024年第1期87-94,共8页
Real-time system timing analysis is crucial for estimating the worst-case execution time(WCET)of a program.To achieve this,static or dynamic analysis methods are used,along with targeted modeling of the actual hardwar... Real-time system timing analysis is crucial for estimating the worst-case execution time(WCET)of a program.To achieve this,static or dynamic analysis methods are used,along with targeted modeling of the actual hardware system.This literature review focuses on calculating WCET for multi-core processors,providing a survey of traditional methods used for static and dynamic analysis and highlighting the major challenges that arise from different program execution scenarios on multi-core platforms.This paper outlines the strengths and weaknesses of current methodologies and offers insights into prospective areas of research on multi-core analysis.By presenting a comprehensive analysis of the current state of research on multi-core processor analysis for WCET estimation,this review aims to serve as a valuable resource for researchers and practitioners in the field. 展开更多
关键词 real-time system worst-case execution time(WCET) multi-core analysis
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Hybridization of Metaheuristics Based Energy Efficient Scheduling Algorithm for Multi-Core Systems
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作者 J.Jean Justus U.Sakthi +4 位作者 K.Priyadarshini B.Thiyaneswaran Masoud Alajmi Marwa Obayya Manar Ahmed Hamza 《Computer Systems Science & Engineering》 SCIE EI 2023年第1期205-219,共15页
The developments of multi-core systems(MCS)have considerably improved the existing technologies in thefield of computer architecture.The MCS comprises several processors that are heterogeneous for resource capacities,... The developments of multi-core systems(MCS)have considerably improved the existing technologies in thefield of computer architecture.The MCS comprises several processors that are heterogeneous for resource capacities,working environments,topologies,and so on.The existing multi-core technology unlocks additional research opportunities for energy minimization by the use of effective task scheduling.At the same time,the task scheduling process is yet to be explored in the multi-core systems.This paper presents a new hybrid genetic algorithm(GA)with a krill herd(KH)based energy-efficient scheduling techni-que for multi-core systems(GAKH-SMCS).The goal of the GAKH-SMCS tech-nique is to derive scheduling tasks in such a way to achieve faster completion time and minimum energy dissipation.The GAKH-SMCS model involves a multi-objectivefitness function using four parameters such as makespan,processor utilization,speedup,and energy consumption to schedule tasks proficiently.The performance of the GAKH-SMCS model has been validated against two datasets namely random dataset and benchmark dataset.The experimental outcome ensured the effectiveness of the GAKH-SMCS model interms of makespan,pro-cessor utilization,speedup,and energy consumption.The overall simulation results depicted that the presented GAKH-SMCS model achieves energy effi-ciency by optimal task scheduling process in MCS. 展开更多
关键词 Task scheduling energy efficiency multi-core systems fitness function MAKESPAN
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Shared Cache Based on Content Addressable Memory in a Multi-Core Architecture
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作者 Allam Abumwais Mahmoud Obaid 《Computers, Materials & Continua》 SCIE EI 2023年第3期4951-4963,共13页
Modern shared-memory multi-core processors typically have shared Level 2(L2)or Level 3(L3)caches.Cache bottlenecks and replacement strategies are the main problems of such architectures,where multiple cores try to acc... Modern shared-memory multi-core processors typically have shared Level 2(L2)or Level 3(L3)caches.Cache bottlenecks and replacement strategies are the main problems of such architectures,where multiple cores try to access the shared cache simultaneously.The main problem in improving memory performance is the shared cache architecture and cache replacement.This paper documents the implementation of a Dual-Port Content Addressable Memory(DPCAM)and a modified Near-Far Access Replacement Algorithm(NFRA),which was previously proposed as a shared L2 cache layer in a multi-core processor.Standard Performance Evaluation Corporation(SPEC)Central Processing Unit(CPU)2006 benchmark workloads are used to evaluate the benefit of the shared L2 cache layer.Results show improved performance of the multicore processor’s DPCAM and NFRA algorithms,corresponding to a higher number of concurrent accesses to shared memory.The new architecture significantly increases system throughput and records performance improvements of up to 8.7%on various types of SPEC 2006 benchmarks.The miss rate is also improved by about 13%,with some exceptions in the sphinx3 and bzip2 benchmarks.These results could open a new window for solving the long-standing problems with shared cache in multi-core processors. 展开更多
关键词 multi-core processor shared cache content addressable memory dual port CAM replacement algorithm benchmark program
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Critical approaches in the catalytic transformation of sugar isomerization and epimerization after Fischer-History,challenges,and prospects
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作者 Da-Ming Gao Xun Zhang +5 位作者 Haichao Liu Hidemi Fujino Tingzhou Lei Fuan Sun Jie Zhu Taoli Huhe 《Green Energy & Environment》 SCIE EI CAS CSCD 2024年第3期435-453,共19页
The transformation of aldose to ketose or common sugars into rare saccharides,including rare ketoses and aldoses,is of great value and interest to the food industry and for saccharidic biomass utilization,medicine,and... The transformation of aldose to ketose or common sugars into rare saccharides,including rare ketoses and aldoses,is of great value and interest to the food industry and for saccharidic biomass utilization,medicine,and the synthesis of drugs.Nowadays,high-fructose corn syrup(HFCS)is industrially produced in more than 10 million tons annually using immobilized glucose isomerase.Some low-calorie saccharides such as tagatose and psicose,which are becoming popular sweeteners,have also been produced on a pilot scale in order to replace sucrose and HFCS.However,current catalysts and catalytic processes are still difficult to utilize in biomass conversion and also have strong substrate dependence in producing high-value,rare sugars.Considering the specific reaction properties of saccharides and catalysts,since the pioneering discovery by Fischer,various catalysts and catalytic systems have been discovered or developed in attempts to extend the reaction pathways,improve the reaction efficiency,and to potentially produce commercial products.In this review,we trace the history of sugar isomerization/epimerization reactions and summarize the important breakthroughs for each reaction as well as the difficulties that remain unresolved to date. 展开更多
关键词 Rare sugars isomerIZATION KETONIZATION EPIMERIZATION Catalytic transformation
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Enrichment of Fetal Nucleated Red Blood Cells by Multi-core Magnetic Composite Particles for Non-invasive Prenatal Diagnosis 被引量:1
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作者 PAN Ying WANG Qing +7 位作者 HUANG Wen-jun QIAO Feng-1i LIU Yu-ping ZHANG Yu-cheng HAI De-yang DU Ying,ting WANG Wen-yue ZHANG Ai-chen 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第3期443-448,共6页
A novel kind of multi-core magnetic composite particles, the surfaces of which were respectively mo- dified with goat-anti-mouse IgG and antitransferrin receptor(anti-CD71), was prepared. The fetal nucleated red blo... A novel kind of multi-core magnetic composite particles, the surfaces of which were respectively mo- dified with goat-anti-mouse IgG and antitransferrin receptor(anti-CD71), was prepared. The fetal nucleated red blood cells(FNRBCs) in the peripheral blood of a gravida were rapidly and effectively enriched and separated by the mo- dified multi-core magnetic composite particles in an external magnetic field. The obtained FNRBCs were used for the identification of the fetal sex by means of fluorescence in situ hybridization(FISH) technique. The results demonstrate that the multi-core magnetic composite particles meet the requirements for the enrichment and speration of FNRBCs with a low concentration and the accuracy of detetion for the diagnosis of fetal sex reached to 95%. Moreover, the obtained FNRBCs were applied to the non-invasive diagnosis of Down syndrome and chromosome 3p21 was de- tected. The above facts indicate that the novel multi-core magnetic composite particles-based method is simple, relia- ble and cost-effective and has opened up vast vistas for the potential application in clinic non-invasive prenatal diag- nosis. 展开更多
关键词 Fetal nucleated red blood cell(FNRBC) Prenatal diagnosis NON-INVASIVE multi-core magnetic compositeparticle
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Study on Isomerous CAD Model Exchange Based on Feature
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作者 SHAO Xiaodong CHEN Feng XU Chenguang (School of Mechano-electronic Engineering,Xidian Univ.,Xi’an 710071,China 《武汉理工大学学报》 CAS CSCD 北大核心 2006年第S2期497-501,共5页
A model-exchange method based on feature between isomerous CAD systems is put forward in this paper.In this method,CAD model information is accessed at both feature and geometry levels and converted according to stand... A model-exchange method based on feature between isomerous CAD systems is put forward in this paper.In this method,CAD model information is accessed at both feature and geometry levels and converted according to standard feature opera- tion.The feature information including feature tree,dimensions and constraints,which will be lost in traditional data conver- sion,as well as geometry are converted completely from source CAD system to destination one.So the transferred model can be edit- ed through feature operation,which cannot be implemented by general model-exchange interface. 展开更多
关键词 INTERFACE MODEL EXCHANGE FEATURE isomerous CAD STANDARD
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Photonuclear production of nuclear isomers using bremsstrahlung induced by laser-wakefield electrons 被引量:3
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作者 Hao-Yang Lan Di Wu +6 位作者 Jia-Xin Liu Jian-Yao Zhang Huan-Gang Lu Jian-Feng Lv Xue-Zhi Wu Wen Luo Xue-Qing Yan 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第5期112-124,共13页
In this study,we theoretically investigate the feasibility of using laser-wakefield accelerated(LWFA)electrons for the photonuclear measurement of nuclear isomers according to the characteristics of the electrons obta... In this study,we theoretically investigate the feasibility of using laser-wakefield accelerated(LWFA)electrons for the photonuclear measurement of nuclear isomers according to the characteristics of the electrons obtained from LWFA experiments conducted at the Compact Laser-Plasma Accelerator(CLAPA)laboratory.The experiments at the CLAPA show that a stable electron beam with an energy of 78-135 MeV and a charge of 300-600 pC can be obtained.The bremsstrahlung spectra were simulated using Geant4,which suggests that a bremsstrahlung source with a peak intensity of 10^(19) photons/s can be generated.Theoretical calculations of isomer production cross sections from the photonuclear reactions on six target nuclei,^(197)Au,^(180)Hf,^(159)Tb,^(115)In,^(103)Rh,and ^(90)Zr,were performed and compared with the available experimental data in EXFOR,which suggest that further experiments are required for a series of photonuclear reaction channels.Flux-averaged cross sections and isomer ratios(IR)resulting from such bremsstrahlung sources are theoretically deduced.The results suggest that IR measurements can be used to constrain nuclear components,such asγstrength function and optical model potential.In addition,the detection of the decay characteristics was evaluated with Geant4 simulations.The use of the LWFA electron beam and its bremsstrahlung for photonuclear studies involving nuclear isomers is anticipated. 展开更多
关键词 Photonuclear reactions Laser–plasma acceleration Flux-averaged isomer ratio
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Variation-Aware Task Mapping on Homogeneous Fault-Tolerant Multi-Core Network-on-Chips
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作者 Chengbo Xue Yougen Xu +1 位作者 Yue Hao Wei Gao 《Journal of Beijing Institute of Technology》 EI CAS 2019年第3期497-509,共13页
A variation-aware task mapping approach is proposed for a multi-core network-on-chips with redundant cores, which includes both the design-time mapping and run-time scheduling algorithms. Firstly, a design-time geneti... A variation-aware task mapping approach is proposed for a multi-core network-on-chips with redundant cores, which includes both the design-time mapping and run-time scheduling algorithms. Firstly, a design-time genetic task mapping algorithm is proposed during the design stage to generate multiple task mapping solutions which cover a maximum range of chips. Then, during the run, one optimal task mapping solution is selected. Additionally, logical cores are mapped to physically available cores. Both core asymmetry and topological changes are considered in the proposed approach. Experimental results show that the performance yield of the proposed approach is 96% on average, and the communication cost, power consumption and peak temperature are all optimized without loss of performance yield. 展开更多
关键词 process VARIATION TASK mapping FAULT-TOLERANT network-on-chips multi-core
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Theoretical Analysis on Inter-Core Crosstalk Suppression Model for Multi-Core Fiber
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作者 Jiajing Tu Xueqin Xie Keping Long 《China Communications》 SCIE CSCD 2016年第8期192-197,共6页
Decreasing mode coupling coefficient(κ) is an effective approach to suppress the inter-core crosstalk. Therefore, we deploy a low index rod and rectangle trench in the middle of two neighboring cores to reduce κ so ... Decreasing mode coupling coefficient(κ) is an effective approach to suppress the inter-core crosstalk. Therefore, we deploy a low index rod and rectangle trench in the middle of two neighboring cores to reduce κ so that the overlap of electric field distribution can be suppressed. We also propose approximate analytical solution(AAS) for κ of two crosstalk suppression models, which are two cores with one low index rod deployed in the middle and two cores with one low index rectangle trench deployed in the middle. We then do some modification for the results obtained by AAS and the modified results are proved to agree well with that obtained by finite element method(FEM). Therefore, we can use the modified AAS to get inter-core crosstalk for abovementioned two models quickly. 展开更多
关键词 multi-core fiber CROSSTALK mode coupling coefficient
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Modeling of Few-Mode Multi-Core Optical Fiber Channe Based on Non-Uniform Mode Field Distribution
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作者 Hang Zhou Bo Liu +6 位作者 Fu Wang Dandan Song Li Li Xiangjun Xin Qinghua Tian Qi Zhang Feng Tian 《China Communications》 SCIE CSCD 2016年第8期184-191,共8页
In this paper, the influencing factors that affect few-mode and multi core optical fiber channel are analyzed in a comprehensive way. The theoretical modeling and computer simulation of the information channel are car... In this paper, the influencing factors that affect few-mode and multi core optical fiber channel are analyzed in a comprehensive way. The theoretical modeling and computer simulation of the information channel are carried out and then the modeling scheme of few-mode multicore optical fiber channel based on non-uniform mode field distribution is put forward. The proposed modeling scheme can not only exponentially increases the system capacity through fewmode multi-core optical fiber channel, but has better transmission performance compared to the channel of the same type to the uniform channel revealing from the simulation results. 展开更多
关键词 few-mode multi-core optical fiber channel non-uniform channel channel modeling
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Performance Analysis for EDMA Based on TIC6678Multi-core DSP
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《信息工程期刊(中英文版)》 2015年第3期73-77,共5页
Frequent data exchange among all kinds of memories has become an inevitable phenomenon in the process of modern embeddedsoftware design. In order to improve the ability of the embedded system data's throughput and co... Frequent data exchange among all kinds of memories has become an inevitable phenomenon in the process of modern embeddedsoftware design. In order to improve the ability of the embedded system data's throughput and computation, most embeddeddevices introduce Enhanced Direct Memory Access (EDMA) data transfer technology. TMS320C6678 is a multi-core DSPproduced by Texas Instruments (TI). There are ten EDMA transmission controllers in the chip for configuration and datatransmissions are allowed to be performed between any two pieces of storage at the same time. This paper expounds the workingmechanism of EDMA based on multi-core DSP TMS320C6678. At the same time, multiple data sets are provided and thebottleneck of limiting data throughout is analyzed and solved. 展开更多
关键词 EDMA multi-core DSP HIGH-SPEED Data THROUGHOUT
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The Channel Maps and the Position-Velocity Diagrams of Multi-core Structure of Cepheus C
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作者 Yu Zhi yao 1,2 , Jiang Dong rong 1,2 1 (Shanghai Astronomical Observatory, The Chinese Academy of Sciences, Shanghai 200030, China) 2 (National Astronomical Observatories, The Chinese Academy of Sciences, China E mail: zyyu@center. shao. ac. 《天文研究与技术》 CSCD 1999年第S1期218-221,共4页
The first important problem in the star forming process is the formation of proto star core in star forming regions of molecular cloud. The multi core structure in star forming regions is related to the forming of pro... The first important problem in the star forming process is the formation of proto star core in star forming regions of molecular cloud. The multi core structure in star forming regions is related to the forming of proto star core. The molecular radiation of C 18 O( J = 1-0) in Cepheus C has been observed. The C 18 O( J = 1-0) observations form the basis for an interesting study on the cloud cores and star formation activity in the cores of the Cepheus C. In order to study the multi core structure of C 18 O( J = 1-0) in the Cepheus C the channel maps and the position velocity diagrams of C 18 O( J = 1-0) will be shown. From the maps it is found that the contour level and distribution size of the three cores in Cepheus C are related to the channel velocity very much. The channel velocity of C 18 O( J = 1-0) molecules in core b, which distributed in all the channels velocity, is different with one in core a and core c very much. The C 18 O( J = 1-0) molecules in core a and core c of the Cepheus C mostly distributed in the blue shifted channel velocity relating to peak velocity, and only in -10.0 ~ -9.5 km/s, which is the red shifted channel velocity relating to peak velocity. And the contour level of C 18 O( J = 1-0) in -10.0 ~ -9.5 km/s is small and the distrbution size in the channel map is small. According to the position velocity diagrams the asymmetry of the distribution both blue shifted and red shifted components should reflect the asymmetry of the profile. From the diagrams it also is found that the contour level and the distribution size of the three cores are different from each other. Both results from the maps and diagrams are coincident with each other. 展开更多
关键词 MAPS The Channel Maps and the Position-Velocity core Diagrams of multi-core Structure of Cepheus C
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Parallel Processing Design for LTE PUSCH Demodulation and Decoding Based on Multi-Core Processor
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作者 Zhang Ziran,Li Jun,Li Changxiao(ZTE Corporation,Shenzhen 518057,P.R.China) 《ZTE Communications》 2009年第1期54-58,共5页
The Long Term Evolution (LTE) system imposes high requirements for dispatching delay.Moreover,very large air interface rate of LTE requires good processing capability for the devices processing the baseband signals.Co... The Long Term Evolution (LTE) system imposes high requirements for dispatching delay.Moreover,very large air interface rate of LTE requires good processing capability for the devices processing the baseband signals.Consequently,the single-core processor cannot meet the requirements of LTE system.This paper analyzes how to use multi-core processors to achieve parallel processing of uplink demodulation and decoding in LTE systems and designs an approach to parallel processing.The test results prove that this approach works quite well. 展开更多
关键词 CORE LTE Parallel Processing Design for LTE PUSCH Demodulation and Decoding Based on multi-core Processor Design
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RF-TSV DESIGN, MODELING AND APPLICATION FOR 3D MULTI-CORE COMPUTER SYSTEMS
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作者 Yu Le Yang Haigang Xie Yuanlu 《Journal of Electronics(China)》 2012年第5期431-444,共14页
The state-of-the-art multi-core computer systems are based on Very Large Scale three Dimensional (3D) Integrated circuits (VLSI). In order to provide high-speed vertical data transmission in such 3D systems, efficient... The state-of-the-art multi-core computer systems are based on Very Large Scale three Dimensional (3D) Integrated circuits (VLSI). In order to provide high-speed vertical data transmission in such 3D systems, efficient Through-Silicon Via (TSV) technology is critically important. In this paper, various Radio Frequency (RF) TSV designs and models are proposed. Specifically, the Cu-plug TSV with surrounding ground TSVs is used as the baseline structure. For further improvement, the dielectric coaxial and novel air-gap coaxial TSVs are introduced. Using the empirical parameters of these coaxial TSVs, the simulation results are obtained demonstrating that these coaxial RF-TSVs can provide two-order higher of cut-off frequencies than the Cu-plug TSVs. Based on these new RF-TSV technologies, we propose a novel 3D multi-core computer system as well as new architectures for manipulating the interfaces between RF and baseband circuit. Taking into consideration the scaling down of IC manufacture technologies, predictions for the performance of future generations of circuits are made. With simulation results indicating energy per bit and area per bit being reduced by 7% and 11% respectively, we can conclude that the proposed method is a worthwhile guideline for the design of future multi-core computer ICs. 展开更多
关键词 Three Dimensional (3D) Very Large Scale Integrated circuits (VLSI) Ratio Frequency (RF) Through-Silicon Vias (TSVs) multi-core computer technology
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Classifying Cardiac Anomalies in Right and Left Isomerism:Concordant and Discordant Patterns
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作者 Lilia Oreto Giuseppe Mandraffino +11 位作者 Paolo Ciliberti Teresa P.Santangelo Placido Romeo Antonio Celona Placido Gitto Lorenzo Galletti Fiore S.Iorio Alfredo Di Pino Aurelio Secinaro Paolo Guccione Robert H.Anderson Salvatore Agati 《Congenital Heart Disease》 SCIE 2023年第1期97-111,共15页
Aims:Evidence is emerging that,in the setting of isomerism,the atrial and bronchial arrangement are not always concordant,nor are these patterns always harmonious with the arrangement of the abdominal organs.We aimed ... Aims:Evidence is emerging that,in the setting of isomerism,the atrial and bronchial arrangement are not always concordant,nor are these patterns always harmonious with the arrangement of the abdominal organs.We aimed to evaluate the concordance between these features in a cohort of patients with cardiac malformations in the setting of known isomerism,seeking to determine whether it was feasible to assess complexity on this basis,in this regard taking note of the potential value of bronchial as opposed to appendage morphology.Methods and Results:We studied 78 patients known to have isomerism of the bronchuses,43 with right and 35 with left isomerism.Appendage anatomy could be determined in 49 cases(63%),all but one of these being concordant with bronchial anatomy.When assessing abdominal features,in only 59 cases(76%)was splenic morphology in keeping with the thoracic findings.As expected,right isomerism was associated with greater complexity of cardiac malformations,with an odds ratio of 6.53,with confidence intervals from 2.2–19.3(p<0.001).The odds were slightly decreased with thoraco-abdominal disharmony,when lesions shown to carry higher risk were then found in the setting of left isomerism.Conclusion:Harmony is excellent between bronchial and appendage isomerism,but less so with the arrangement of the abdominal organs.Right isomerism in our cohort,was indicative of a sixfold increase in intracardiac complexity.When discordance was found between the systems,however,the cardiac anomalies were less typical of the anticipated findings for right vs.left isomerism of the appendages. 展开更多
关键词 isomerISM ASPLENIA polysplenia congenital heart disease bronchial isomerism atrial appendages
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Synthesis of MgO-doped ordered mesoporous carbons by Mg^(2+)-tannin coordination for efficient isomerization of glucose to fructose
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作者 Junyan Fu Feng Shen +1 位作者 Xiaoning Liu Xinhua Qi 《Green Energy & Environment》 SCIE EI CAS CSCD 2023年第3期842-851,共10页
In-situ MgO-doped ordered mesoporous carbon(OMC@MgO)was fabricated by formaldehyde-free self-assembly method,in which biomass-derived tannin was used as carbon precursor replacing fossil-based phenolics,Mg^(2+)as both... In-situ MgO-doped ordered mesoporous carbon(OMC@MgO)was fabricated by formaldehyde-free self-assembly method,in which biomass-derived tannin was used as carbon precursor replacing fossil-based phenolics,Mg^(2+)as both cross-linker and precursor of catalytic sites.Up to~20 wt% MgO could be doped in the carbon skeleton with good dispersion retaining well-ordered mesoporous structures,while more MgO content(35 wt%)led to the failing in the formation of ordered mesoporous structure.The OMC@MgO possessed a high specific surface area(298.8 m^(2) g^(-1)),uniform pore size distribution(4.8 nm)and small crystallite size of MgO(1.73 nm)due to the confinement effect of ordered mesoporous structure.Using OMC@MgO as the heterogeneous catalyst,a maximum fructose yield of 32.4% with a selectivity up to 81.1%was achieved from glucose in water(90℃,60 min),which is much higher than that obtained using the MgO doped active carbon via conventional post-impregnation method(26.5%yield with 58.3% selectivity).Higher reaction temperature(>90℃)resulted in decrease of selectivity due to the formation of humins.The designed OMC@MgO displayed tolerant to high initial glucose concentrations(10 wt%)and could remain good recyclability without significant loss of activity for three cycles. 展开更多
关键词 GLUCOSE FRUCTOSE isomerIZATION Porous carbon Biomass CATALYSIS
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Fragmentation stability and retention time-shift obtained by LC-MS/MS to distinguish sialylated N-glycan linkage isomers in therapeutic glycoproteins
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作者 Chi Soo Park Minju Kang +7 位作者 Ahyeon Kim Chulmin Moon Mirae Kim Jieun Kim Subin Yang Leeseul Jang Ji Yeon Jang Ha Hyung Kim 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第3期305-314,共10页
Sialylated N-glycan isomers withα2-3 orα2-6 linkage(s)have distinctive roles in glycoproteins,but are difficult to distinguish.Wild-type(WT)and glycoengineered(mutant)therapeutic glycoproteins,cytotoxic T lymphocyte... Sialylated N-glycan isomers withα2-3 orα2-6 linkage(s)have distinctive roles in glycoproteins,but are difficult to distinguish.Wild-type(WT)and glycoengineered(mutant)therapeutic glycoproteins,cytotoxic T lymphocyte-associated antigen-4-immunoglobulin(CTLA4-Ig),were produced in Chinese hamster ovary cell lines;however,their linkage isomers have not been reported.In this study,N-glycans of CTLA4-Igs were released,labeled with procainamide,and analyzed by liquid chromatography-tandem mass spectrometry(MS/MS)to identify and quantify sialylated N-glycan linkage isomers.The linkage isomers were distinguished by comparison of 1)intensity of the N-acetylglucosamine ion to the sialic acid ion(Ln/Nn)using different fragmentation stability in MS/MS spectra and 2)retention time-shift for a selective m/z value in the extracted ion chromatogram.Each isomer was distinctively identified,and each quantity(>0.1%)was obtained relative to the total N-glycans(100%)for all observed ionization states.Twenty sialylated N-glycan isomers with onlyα2-3 linkage(s)in WT were identified,and each isomer's sum of quantities was 50.4%.Furthermore,39 sialylated N-glycan isomers(58.8%)in mono-(3 N-glycans;0.9%),bi-(18;48.3%),tri-(14;8.9%),and tetra-(4;0.7%)antennary structures of mutant were obtained,which comprised mono-(15 N-glycans;25.4%),di-(15;28.4%),tri-(8;4.8%),and tetra-(1;0.2%)sialylation,respectively,with onlyα2-3(10 N-glycans;4.8%),bothα2-3 andα2-6(14;18.4%),and onlyα2-6(15;35.6%)linkage(s).These results are consistent with those forα2-3 neuraminidase-treated N-glycans.This study generated a novel plot of Ln/Nn versus retention time to distinguish sialylated N-glycan linkage isomers in glycoprotein. 展开更多
关键词 Therapeutic glycoprotein SIALYLATION Linkage isomer LC-MS/MS
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A novel synthesis of highly active and highly stable non-noble-nickel-modified persulfated Al_(2)O_(3)@ZrO_(2) core-shell catalysts for n-pentane isomerization
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作者 Tian-Han Zhu Mei Zhang +2 位作者 Sheng-Nan Li Feng Li Hua Song 《Petroleum Science》 SCIE EI CAS CSCD 2023年第4期2545-2553,共9页
The non-noble metal modified sulfated zirconia was found easy to deactivate.Herein,highly active and highly stable non-noble core-shell Ni-S_(2)O_(8)^(2−)/Al_(2)O_(3)@ZrO_(2) catalysts(Ni-SA@Z-x,x=Al content in wt%)ha... The non-noble metal modified sulfated zirconia was found easy to deactivate.Herein,highly active and highly stable non-noble core-shell Ni-S_(2)O_(8)^(2−)/Al_(2)O_(3)@ZrO_(2) catalysts(Ni-SA@Z-x,x=Al content in wt%)have been successfully prepared and investigated for n-pentane isomerization.The results showed that the core-shell Ni-SA@Z-30 provided a sustained high isopentane yield(63.1%)with little or no deactivation within 5000 min at a mild reaction pressure of 2.0 MPa,which can be attributed to the following factors:(i)carbon deposition was greatly suppressed by the large pore size and huge pore volume;(ii)the loss of sulfur entities was suppressed because the small and highly dispersed tetragonal ZrO_(2) particles can bond with the S species strongly;(iii)strong Brønsted acidity can be maintained well after the isomerization.The pore structures and acid nature of the core-shell Ni-SA@Z-x are entirely different from those of the normal structure Ni-S_(2)O_(8)^(2−)/ZrO_(2)-Al_(2)O_(3),even though the Al content and the compositions of the individual components are the same.The Al_(2)O_(3)cores endow the catalysts with high internal surface area and high mechanical strength.Meanwhile,the ZrO_(2) shell,which consists of more and smaller tetragonal ZrO_(2) particles because of the large surface area of the Al_(2)O_(3)core,promotes the formation of more stable sulfur species and stronger binding sites. 展开更多
关键词 CORE-SHELL Large pore size Solid superacid Non-noble metal Ni isomerIZATION
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Quantum Dynamics Calculations on Isotope Effects of Hydrogen Transfer Isomerization in Formic Acid Dimer
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作者 Fengyi Li Xiaoxi Liu +2 位作者 Xingyu Yang Jianwei Cao Wensheng Bian 《Chinese Journal of Chemical Physics》 SCIE EI CAS CSCD 2023年第5期545-552,I0001,共9页
We present a quantum dynamics study on the isotope effects of hydro-gen transfer isomerization in the formic acid dimer,and this is achieved by multidimensional dy-namics calculations with an efficient quantum mechani... We present a quantum dynamics study on the isotope effects of hydro-gen transfer isomerization in the formic acid dimer,and this is achieved by multidimensional dy-namics calculations with an efficient quantum mechanical theoretical scheme developed by our group,on a full-dimensional neural network ab initio potential energy surface.The ground-state and fundamental tun-neling splittings for four deuterium isotopologues of formic acid dimer are considered,and the calculated results are in very good general agreement with the avail-able experimental measurements.Strong isotope effects are revealed,the mode-specific funda-mental excitation effects on the tunneling rate are evidently influenced by the deuterium sub-stitution of H atom with the substitution on the OH bond being more effective than on the CH bond.Our studies are helpful for acquiring a better understanding of isotope effects in the double-hydrogen transfer processes. 展开更多
关键词 Quantum dynamics isomerIZATION Isotope effect Tunneling splitting Double hydrogen transfer
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Seed-solution-induced Synthesis of FER Zeolite and Its Catalytic Application in the Skeletal Isomerization of n-Butene
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作者 Fan Jinlong Yang Fan +2 位作者 Zhu Xuedong Xu Yarong Chen Lantian 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS CSCD 2023年第4期61-73,共13页
A series of Ferrierite(FER)zeolites were prepared via hydrothermal synthesis in the absence of organic templates with the aid of sodium-type FER zeolite(NaFER)or NaFER suspensions(NaFERsus)acquired by NaOH solution tr... A series of Ferrierite(FER)zeolites were prepared via hydrothermal synthesis in the absence of organic templates with the aid of sodium-type FER zeolite(NaFER)or NaFER suspensions(NaFERsus)acquired by NaOH solution treatment as seeds.The differences in the structures and acid sites of the obtained FER zeolite catalysts arising from the choice of seed were investigated,and the catalytic performances of the obtained FER zeolites were evaluated in the skeletal isomerization of n-butene.The results indicate that the samples synthesized using NaFERsus feature more Br?nsted acid sites(BAS)in the 10-membered-ring(10-MR)at the expense of strong acid and Lewis acid sites(LAS),compared with samples derived from NaFER.Therefore,the FER samples synthesized using NaFERsus outperformed the NaFER counterparts as the BAS in 10-MR and LAS were the main active sites,while BAS in 8-MR and LAS were responsible for side reactions,such as polymerization,cracking,and carbon deposition in n-butene isomerization.The optimized FER catalyst was continuously used for 720 h at 350℃ at 0.1 MPa under an n-butene space velocity of 2.0 h^(-1),during which the n-butene conversion remained at>40%,and the isobutylene yield was>37.5%. 展开更多
关键词 FER zeolites alkali treated seed solution N-BUTENE skeletal isomerization
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