Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this s...Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this study,a highly male-sterile line Gao146A was investigated.Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene.Using F 2 population derived from cross Gao146A/K103,one gene controlling the highly male- sterility,tentatively named as ms1,which linked to SSR marker b234 with genetic distance of 16.7 cM,was mapped on the chromosome VI.These results not only laid the foundation for fine mapping of this highly male-sterile gene,but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.展开更多
Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important...Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability.展开更多
Foxtail millet(Setaria italica(L.)P.Beauv)is a naturally stress tolerant crop.Compared to other gramineous crops,it has relatively stronger drought and lower nutrition stress tolerance traits.To date,the scope of ...Foxtail millet(Setaria italica(L.)P.Beauv)is a naturally stress tolerant crop.Compared to other gramineous crops,it has relatively stronger drought and lower nutrition stress tolerance traits.To date,the scope of functional genomics research in foxtail millet(S.italic L.)has been quite limited.NAC(NAM,ATAF1/2 and CUC2)-like transcription factors are known to be involved in various biological processes,including abiotic stress responses.In our previous foxtail millet(S.italic L.)RNA seq analysis,we found that the expression of a NAC-like transcription factor,SiNAC110,could be induced by drought stress;additionally,other references have reported that SiNAC110 expression could be induced by abiotic stress.So,we here selected SiNAC110 for further characterization and functional analysis.First,the predicted SiNAC110 protein encoded indicated SiNAC110 has a conserved NAM(no apical meristem)domain between the 11–139 amino acid positions.Phylogenetic analysis then indicated that SiNAC110 belongs to subfamily III of the NAC gene family.Subcellular localization analysis revealed that the SiNAC110-GFP fusion protein was localized to the nucleus in Arabidopsis protoplasts.Gene expression profiling analysis indicated that expression of SiNAC110 was induced by dehydration,high salinity and other abiotic stresses.Gene functional analysis using SiNAC110 overexpressed Arabidopsis plants indicated that,under drought and high salt stress conditions,the seed germination rate,root length,root surface area,fresh weight,and dry weight of the SiNAC110 overexpressed lines were significantly higher than the wild type(WT),suggesting that the SiNAC110 overexpressed lines had enhanced tolerance to drought and high salt stresses.However,overexpression of SiN AC110 did not affect the sensitivity of SiNAC110 overexpressed lines to abscisic acid(ABA)treatment.Expression analysis of genes involved in proline synthesis,Na+/K+transport,drought responses,and aqueous transport proteins were higher in the SiNAC110overexpressed lines than in the WT,whereas expression of ABA-dependent pathway genes did not change.These results indicated that overexpression of SiNAC110 conferred tolerance to drought and high salt stresses,likely through influencing the regulation of proline biosynthesis,ion homeostasis and osmotic balance.Therefore,SiNAC110 appears to function in the ABA-independent abiotic stress response pathway in plants.展开更多
In this study, the plant biomass production, biomass translocation rates across tissues and the lodging resistant-associated traits of millet ( Setaria italica L.) in North China were investigated. Among the four su...In this study, the plant biomass production, biomass translocation rates across tissues and the lodging resistant-associated traits of millet ( Setaria italica L.) in North China were investigated. Among the four summer millet cultivars, Baogu 19 exhibited improved plant biomass (PB) production at flowering and maturity stages, biomass translocation amount (BTA) from vegetative tissues to seeds during filling period, and lodging resistant-associated (LRA) traits compared with other cultivars, including enhanced stem lignin contents, increased anti-broken resistance (ABR), anti-puncturing resistance (APR), and stem diameter (SD) of plants. Compared with treatment regular cultivation (RC), high fertility treatment (HF) increased the plant BP, BTA from vegetative tissue to seed at filling stage, and the plant LRA traits; whereas high density treatment (HD) decreased the plant BP at plant level, plant BTA from vegetative tissues to seeds at filling stage, and the plant LRA traits. Correlation analysis revealed that stem ABR is significantly correlated with the plant lodging resistant-associated traits including APR and SD in the summer millet cultivars examined under various cultivation treatments. Our investigation indicates that cultivar Baogu 19 together with suitable fertilization and density can promote the plant biomass production, enhance vegetative tissue biomass translocation to seeds, and improve the lodging resistance of summer millet plants in North China.展开更多
为探讨谷子(Setaria italica L.)耐旱抗逆机制,解析类受体蛋白激酶(receptor like protein kinase,RLKs)基因功能,进而为培育谷子抗逆新品种提供依据,本文以干旱处理的谷子"豫谷1号"为材料,通过i TRAQ技术筛选到1个干旱响应...为探讨谷子(Setaria italica L.)耐旱抗逆机制,解析类受体蛋白激酶(receptor like protein kinase,RLKs)基因功能,进而为培育谷子抗逆新品种提供依据,本文以干旱处理的谷子"豫谷1号"为材料,通过i TRAQ技术筛选到1个干旱响应的类受体蛋白激酶基因,命名为SiRLK35。以谷子RNA反转录的单链cDNA为模板,经PCR扩增获取SiRLK35基因全长序列。应用qRT-PCR方法,对SiRLK35在NaCl、PEG、ABA、GA、Me JA等不同处理下的表达模式进行分析。进一步构建基因原核表达载体pET28a-SiRLK35,结合斑点法对SiRLK35的抗盐能力进行初步评价。同时构建过表达载体p CAMBIA1301P-SiRLK35转化水稻,并对转基因植株抗盐能力进行检测。结果显示:胁迫及激素处理均可不同程度诱导SiRLK35基因的表达;斑点法研究结果显示,在相同NaCl浓度的LB平板上,含有SiRLK35基因的原核表达载体的大肠杆菌菌株生长状态较阴性对照好,SiRLK35具有一定的抗盐能力;获得的转SiRLK35基因水稻植株对盐胁迫的耐受性高于对照。SiRLK35基因对不同胁迫均可以产生响应,但对盐胁迫的响应较为明显,推测该基因可能在谷子的抗盐及抗逆过程中发挥作用。展开更多
Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the...Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli (L.) Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.展开更多
A new millet (Setaria italica Beauv) variety, super early-mature millet No.1, was bred by means of gene bank breedingmethod of target characters. This variety has the following outstanding characters. (1) Super early-...A new millet (Setaria italica Beauv) variety, super early-mature millet No.1, was bred by means of gene bank breedingmethod of target characters. This variety has the following outstanding characters. (1) Super early-mature. This varietyonly needs 1550C effective accumulated temperature and can normally maturate in the Bashang Region in Hebei Provinceof Chi na, which can break through the limit zone of millet cultivation and move the cultivation zone northward greatly. (2)Multi-spikes, in addition to the effect tilling at the top, the nodes in the low-middle part also can produce spikes. (3) Sweetstem have high sugar content. The contents of whole-sugar, soluable sugar and deoxidized sugar are 74.8, 200.5, 237.2%higher than the regular varieties respectively. (4) High gross protein content. The content of gross protein is higher thanthe regular varieties by 3.9-30.4%. (5)Changeable grain color. The grain color of super early-mature millet No.1 is red inShijiazhuang, but yellow in the Bashang region. In addition, this variety is characterized by good quality, high yield, andgood synthetic traits展开更多
[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis ( L. ) Beauv. ], so as to solve the problem of serious damage caused by gr...[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis ( L. ) Beauv. ], so as to solve the problem of serious damage caused by green foxtail. [Method] Using the orthogenal test and the LSD method of software SPSS 18.0, with the number of emerged seedlings of green foxtail as the evaluation index, the influencing factors such as pretreatment of green foxtail, earth covering depth, soil temperature and soil moisture content were compared in the test. [Result] These four experimental factors had great impact on seed germination characteristics of green foxtail. The optimal scheme for seedling emergence of green foxtail was as follows: pretreatment of green foxtail for 3 d, earth covering depth 0.5 cm, soil temperature 32 ℃, soil moisture content 15%. The optimum condition was consistent with the actual situation of serious damage of green foxtail ( suffering moderate and heavy rainfall after sowing and before seedling of millet ). Avoiding sowing millet before moderate and heavy rainfall could effectively control serious damage of green fox'tail. [ Conclusion] The study provided theoretical support for effective control against the vicious weed green foxtail.展开更多
基金supported by the Postdoctoral Management Committee,China(92948)the Natural Science Foundation of Shanxi Province,China(2012011032-1)the Chinese Agricultural Research System(CARS-07)
文摘Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this study,a highly male-sterile line Gao146A was investigated.Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene.Using F 2 population derived from cross Gao146A/K103,one gene controlling the highly male- sterility,tentatively named as ms1,which linked to SSR marker b234 with genetic distance of 16.7 cM,was mapped on the chromosome VI.These results not only laid the foundation for fine mapping of this highly male-sterile gene,but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.
文摘Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability.
基金funded by the National Key Project for Research on Transgenic Biology, China (2016ZX08002-002)the Innovation Project of Chinese Academy of Agricultural Sciences
文摘Foxtail millet(Setaria italica(L.)P.Beauv)is a naturally stress tolerant crop.Compared to other gramineous crops,it has relatively stronger drought and lower nutrition stress tolerance traits.To date,the scope of functional genomics research in foxtail millet(S.italic L.)has been quite limited.NAC(NAM,ATAF1/2 and CUC2)-like transcription factors are known to be involved in various biological processes,including abiotic stress responses.In our previous foxtail millet(S.italic L.)RNA seq analysis,we found that the expression of a NAC-like transcription factor,SiNAC110,could be induced by drought stress;additionally,other references have reported that SiNAC110 expression could be induced by abiotic stress.So,we here selected SiNAC110 for further characterization and functional analysis.First,the predicted SiNAC110 protein encoded indicated SiNAC110 has a conserved NAM(no apical meristem)domain between the 11–139 amino acid positions.Phylogenetic analysis then indicated that SiNAC110 belongs to subfamily III of the NAC gene family.Subcellular localization analysis revealed that the SiNAC110-GFP fusion protein was localized to the nucleus in Arabidopsis protoplasts.Gene expression profiling analysis indicated that expression of SiNAC110 was induced by dehydration,high salinity and other abiotic stresses.Gene functional analysis using SiNAC110 overexpressed Arabidopsis plants indicated that,under drought and high salt stress conditions,the seed germination rate,root length,root surface area,fresh weight,and dry weight of the SiNAC110 overexpressed lines were significantly higher than the wild type(WT),suggesting that the SiNAC110 overexpressed lines had enhanced tolerance to drought and high salt stresses.However,overexpression of SiN AC110 did not affect the sensitivity of SiNAC110 overexpressed lines to abscisic acid(ABA)treatment.Expression analysis of genes involved in proline synthesis,Na+/K+transport,drought responses,and aqueous transport proteins were higher in the SiNAC110overexpressed lines than in the WT,whereas expression of ABA-dependent pathway genes did not change.These results indicated that overexpression of SiNAC110 conferred tolerance to drought and high salt stresses,likely through influencing the regulation of proline biosynthesis,ion homeostasis and osmotic balance.Therefore,SiNAC110 appears to function in the ABA-independent abiotic stress response pathway in plants.
基金Supported by Special Fund of Agricultural Science and Technology Achievement Transformation in Hebei Province(17826335D)Baoding Comprehensive Experimental Station of National Millet and Sorghum Industry Technology System(CARS-06-13.5-B2)
文摘In this study, the plant biomass production, biomass translocation rates across tissues and the lodging resistant-associated traits of millet ( Setaria italica L.) in North China were investigated. Among the four summer millet cultivars, Baogu 19 exhibited improved plant biomass (PB) production at flowering and maturity stages, biomass translocation amount (BTA) from vegetative tissues to seeds during filling period, and lodging resistant-associated (LRA) traits compared with other cultivars, including enhanced stem lignin contents, increased anti-broken resistance (ABR), anti-puncturing resistance (APR), and stem diameter (SD) of plants. Compared with treatment regular cultivation (RC), high fertility treatment (HF) increased the plant BP, BTA from vegetative tissue to seed at filling stage, and the plant LRA traits; whereas high density treatment (HD) decreased the plant BP at plant level, plant BTA from vegetative tissues to seeds at filling stage, and the plant LRA traits. Correlation analysis revealed that stem ABR is significantly correlated with the plant lodging resistant-associated traits including APR and SD in the summer millet cultivars examined under various cultivation treatments. Our investigation indicates that cultivar Baogu 19 together with suitable fertilization and density can promote the plant biomass production, enhance vegetative tissue biomass translocation to seeds, and improve the lodging resistance of summer millet plants in North China.
文摘为探讨谷子(Setaria italica L.)耐旱抗逆机制,解析类受体蛋白激酶(receptor like protein kinase,RLKs)基因功能,进而为培育谷子抗逆新品种提供依据,本文以干旱处理的谷子"豫谷1号"为材料,通过i TRAQ技术筛选到1个干旱响应的类受体蛋白激酶基因,命名为SiRLK35。以谷子RNA反转录的单链cDNA为模板,经PCR扩增获取SiRLK35基因全长序列。应用qRT-PCR方法,对SiRLK35在NaCl、PEG、ABA、GA、Me JA等不同处理下的表达模式进行分析。进一步构建基因原核表达载体pET28a-SiRLK35,结合斑点法对SiRLK35的抗盐能力进行初步评价。同时构建过表达载体p CAMBIA1301P-SiRLK35转化水稻,并对转基因植株抗盐能力进行检测。结果显示:胁迫及激素处理均可不同程度诱导SiRLK35基因的表达;斑点法研究结果显示,在相同NaCl浓度的LB平板上,含有SiRLK35基因的原核表达载体的大肠杆菌菌株生长状态较阴性对照好,SiRLK35具有一定的抗盐能力;获得的转SiRLK35基因水稻植株对盐胁迫的耐受性高于对照。SiRLK35基因对不同胁迫均可以产生响应,但对盐胁迫的响应较为明显,推测该基因可能在谷子的抗盐及抗逆过程中发挥作用。
基金supported by the National Natural Science Foundation of China (31271999)the Special Fund for Agro-Scientific Research in the Public Interest, China (200903039-5)
文摘Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli (L.) Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.
基金This work was supported by the National 863 Program of China(2001AA241251).
文摘A new millet (Setaria italica Beauv) variety, super early-mature millet No.1, was bred by means of gene bank breedingmethod of target characters. This variety has the following outstanding characters. (1) Super early-mature. This varietyonly needs 1550C effective accumulated temperature and can normally maturate in the Bashang Region in Hebei Provinceof Chi na, which can break through the limit zone of millet cultivation and move the cultivation zone northward greatly. (2)Multi-spikes, in addition to the effect tilling at the top, the nodes in the low-middle part also can produce spikes. (3) Sweetstem have high sugar content. The contents of whole-sugar, soluable sugar and deoxidized sugar are 74.8, 200.5, 237.2%higher than the regular varieties respectively. (4) High gross protein content. The content of gross protein is higher thanthe regular varieties by 3.9-30.4%. (5)Changeable grain color. The grain color of super early-mature millet No.1 is red inShijiazhuang, but yellow in the Bashang region. In addition, this variety is characterized by good quality, high yield, andgood synthetic traits
基金Supported by Science and Technology Support Program of Hebei Province(09250307D)Special Fund for Agro-scientific Research in the Public Interest(20120304201)
文摘[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis ( L. ) Beauv. ], so as to solve the problem of serious damage caused by green foxtail. [Method] Using the orthogenal test and the LSD method of software SPSS 18.0, with the number of emerged seedlings of green foxtail as the evaluation index, the influencing factors such as pretreatment of green foxtail, earth covering depth, soil temperature and soil moisture content were compared in the test. [Result] These four experimental factors had great impact on seed germination characteristics of green foxtail. The optimal scheme for seedling emergence of green foxtail was as follows: pretreatment of green foxtail for 3 d, earth covering depth 0.5 cm, soil temperature 32 ℃, soil moisture content 15%. The optimum condition was consistent with the actual situation of serious damage of green foxtail ( suffering moderate and heavy rainfall after sowing and before seedling of millet ). Avoiding sowing millet before moderate and heavy rainfall could effectively control serious damage of green fox'tail. [ Conclusion] The study provided theoretical support for effective control against the vicious weed green foxtail.