AIM:To investigate the presence of mixed infection and discrepancy between hepatitis C virus(HCV) genotypes in plasma,peripheral blood mononuclear cells(PBMCs),and liver biopsy specimens.METHODS:From September 2008 up...AIM:To investigate the presence of mixed infection and discrepancy between hepatitis C virus(HCV) genotypes in plasma,peripheral blood mononuclear cells(PBMCs),and liver biopsy specimens.METHODS:From September 2008 up to April 2009,133 patients with chronic hepatitis C referred to Firouzgar Hospital for initiation of an antiviral therapy were recruited in the study.Five milliliters of peripheral blood was collected from each patient and liver biopsy was performed in those who gave consent or had indications.HCV genotyping was done using INNO-LiPATM HCV in serum,PBMCs,and liver biopsy specimens and then conf irmed by sequencing of 5'-UTR fragments.RESULTS:The mean age of patients was 30.3 ± 17.1 years.Multiple transfusion was seen in 124(93.2%) of patients.Multiple HCV genotypes were found in 3(2.3%) of 133 plasma samples,9(6.8%) of 133 PBMC samples,and 8(18.2%) of 44 liver biopsy specimens.It is notable that the different genotypes found in PBMCs were not the same as those found in plasma and liver biopsy specimens.CONCLUSION:Our study shows that a signif icant proportion of patients with chronic hepatitis C are affected by multiple HCV genotypes which may not be detectable only in serum of patients.展开更多
常见的It is/was…that…句型大致可分为两种;一种是:It is/Was adj that… 例如:Indeed,it is most likely that school-children who are alive now will read of successful journeys to other planets before very long. 另一种是:I...常见的It is/was…that…句型大致可分为两种;一种是:It is/Was adj that… 例如:Indeed,it is most likely that school-children who are alive now will read of successful journeys to other planets before very long. 另一种是:It is/was focused part that… 例如:In England,it is social system that determines who you are. 这两种句型从结构上来说有许多相似之处,但有很多区别,试分析如下. I.It is/was adj that…的结构分析:展开更多
Aim:Peripheral cytokines contribute to arthritis and bone cancer pain through sensory nerve actions.However,increased spinal cytokine and glial filament expression,coined neuroinflammation,has also been proposed to pl...Aim:Peripheral cytokines contribute to arthritis and bone cancer pain through sensory nerve actions.However,increased spinal cytokine and glial filament expression,coined neuroinflammation,has also been proposed to play a part in chronic pain.Therefore,spinal cord,dorsal root ganglia and circulating cytokines were compared in murine arthritis and bone cancer models in relationship to behavioral signs of pain.Methods:Exploratory behaviors were studied after intra-articular complete Freund’s adjuvant or bone intramedullary sarcoma cell injection.Nervous tissue and blood cytokine expression were determined by real-time polymerase chain reaction(PCR)and multiplex immunoassays,respectively.Results:PCR analysis did not reveal any hallmark of spinal neuroinflammation in spontaneously-behaving mice with cartilage or bone lesions.However,imposed paw stimulation during joint inflammation increased spinal interleukin-1β(IL-1β)expression.Spontaneous paw guarding during rearing was displayed by animals with joint inflammation and bone destruction and was accompanied by increased circulating IL-6 and monocyte chemoattractant protein-1,respectively.In addition,dorsal root ganglia were found to constitutively express receptors for this chemotactic cytokine.Conclusion:Our findings indicate that spinal neuroinflammation is not a necessary condition for chronic pain and suggest that circulating cytokine action in dorsal root ganglia may contribute to experimental joint inflammation and bone cancer pain.展开更多
通过RFID识别技术和Wi-Fi/GPS的定位、导航技术,建立物流供应链仓储和运输单元的物流跟踪信息系统(Logistics tracking information system,简称LTIS),并以此为基础,结合其他成熟的通信技术手段,通过耦合和组网的方式,提出一种基于组网...通过RFID识别技术和Wi-Fi/GPS的定位、导航技术,建立物流供应链仓储和运输单元的物流跟踪信息系统(Logistics tracking information system,简称LTIS),并以此为基础,结合其他成熟的通信技术手段,通过耦合和组网的方式,提出一种基于组网的物流跟踪和通信信息系统(Logistics tracking and communications information systems简称LTCIS)。展开更多
基金Supported by Local Fund from Iran University of Medical Sciences
文摘AIM:To investigate the presence of mixed infection and discrepancy between hepatitis C virus(HCV) genotypes in plasma,peripheral blood mononuclear cells(PBMCs),and liver biopsy specimens.METHODS:From September 2008 up to April 2009,133 patients with chronic hepatitis C referred to Firouzgar Hospital for initiation of an antiviral therapy were recruited in the study.Five milliliters of peripheral blood was collected from each patient and liver biopsy was performed in those who gave consent or had indications.HCV genotyping was done using INNO-LiPATM HCV in serum,PBMCs,and liver biopsy specimens and then conf irmed by sequencing of 5'-UTR fragments.RESULTS:The mean age of patients was 30.3 ± 17.1 years.Multiple transfusion was seen in 124(93.2%) of patients.Multiple HCV genotypes were found in 3(2.3%) of 133 plasma samples,9(6.8%) of 133 PBMC samples,and 8(18.2%) of 44 liver biopsy specimens.It is notable that the different genotypes found in PBMCs were not the same as those found in plasma and liver biopsy specimens.CONCLUSION:Our study shows that a signif icant proportion of patients with chronic hepatitis C are affected by multiple HCV genotypes which may not be detectable only in serum of patients.
文摘常见的It is/was…that…句型大致可分为两种;一种是:It is/Was adj that… 例如:Indeed,it is most likely that school-children who are alive now will read of successful journeys to other planets before very long. 另一种是:It is/was focused part that… 例如:In England,it is social system that determines who you are. 这两种句型从结构上来说有许多相似之处,但有很多区别,试分析如下. I.It is/was adj that…的结构分析:
文摘Aim:Peripheral cytokines contribute to arthritis and bone cancer pain through sensory nerve actions.However,increased spinal cytokine and glial filament expression,coined neuroinflammation,has also been proposed to play a part in chronic pain.Therefore,spinal cord,dorsal root ganglia and circulating cytokines were compared in murine arthritis and bone cancer models in relationship to behavioral signs of pain.Methods:Exploratory behaviors were studied after intra-articular complete Freund’s adjuvant or bone intramedullary sarcoma cell injection.Nervous tissue and blood cytokine expression were determined by real-time polymerase chain reaction(PCR)and multiplex immunoassays,respectively.Results:PCR analysis did not reveal any hallmark of spinal neuroinflammation in spontaneously-behaving mice with cartilage or bone lesions.However,imposed paw stimulation during joint inflammation increased spinal interleukin-1β(IL-1β)expression.Spontaneous paw guarding during rearing was displayed by animals with joint inflammation and bone destruction and was accompanied by increased circulating IL-6 and monocyte chemoattractant protein-1,respectively.In addition,dorsal root ganglia were found to constitutively express receptors for this chemotactic cytokine.Conclusion:Our findings indicate that spinal neuroinflammation is not a necessary condition for chronic pain and suggest that circulating cytokine action in dorsal root ganglia may contribute to experimental joint inflammation and bone cancer pain.
文摘通过RFID识别技术和Wi-Fi/GPS的定位、导航技术,建立物流供应链仓储和运输单元的物流跟踪信息系统(Logistics tracking information system,简称LTIS),并以此为基础,结合其他成熟的通信技术手段,通过耦合和组网的方式,提出一种基于组网的物流跟踪和通信信息系统(Logistics tracking and communications information systems简称LTCIS)。
