[ Objective ] The study aimed to screen rational seedling raising date and seedling raising mode for plateau japonica rice, in order to reduce the effect of low temperature on seedlings. [ Method ] The seedlings of co...[ Objective ] The study aimed to screen rational seedling raising date and seedling raising mode for plateau japonica rice, in order to reduce the effect of low temperature on seedlings. [ Method ] The seedlings of conventional japonica rice Hexi 22-2 and giant embryo No. 1 japonica rice were treated at day tempera- ture 12℃/night temperature 8℃ and day temperature 14 ℃/night temperature 10 ℃, and the indicators including chlorophyll content, soluble sugar content, sol- uble protein content, survival rate, plant height, number of ≥ 1 cm roots, leaf wilting degree and aboveground dry matter weight were determined after treated for 0, 3 and 6 d, respectively. [ Result] Chlorophyll content of seedlings decreased when treated by low temperature for a certain period; with the prolongation of treat- ment time, contents of soluble sugar and soluble protein in some low temperature treatments increased, while plant height, number of ≥ 1 cm roots and aboveground dry matter weight increased slowly, but the growth rate was significantly lower than that at room temperature; with the decrease of temperature and the prolongation of treatment time, leaf wilting degree increased. The comprehensive experimental results showed that with the increasing duration of low temperature, when two japonica rice varieties grew under the same low temperature, seedlings grew more and more slowly, probably because the lower the treatment temperature, the greater the damage on plants. Giant Embryo No. 1 had weaker cold tolerance than Hexi 22-2. [ Conclusion] The study provides a theoretical basis for formula- tion of seedling raising technique of plateau japonica rice.展开更多
In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studi...In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.展开更多
[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of ...[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of rice brown planthoppor were released to super indiea and japonica hybrid flee Yongyou 6 during fillering, booting and gain filling stage, respectively. The reproductive rate and variation dynamics of rice brown planthoppor in super rice was also investigated and analyzed. [Result] Tillering stage: the population quantity of rice brown planthoppor during booting and gain filling stage gradually increased with the increasing basal amount of its population during tillering stage; when the population density of rice brown planthopper during grain filling stage reached a certain limit ( 〉 250 head/cluster), the population density during milking stage showed decrease trend with the increasing of such density; the development of rice brown planthoppor population in super rice Yongyou 6 showed the characteristics of fast in middle and slow in two ends. Booting stage: the development of rice brown planthopper pop- ulation from booting to gain filling stage increased with the increasing basal amount of density, and their correlation during milking stage was not significant; flee brown planthopper population still had high reproduction capacity in the middle and late stage of super rice, which had latent risk on rice yield. Gain filling stage : when population density of rice brown planthopper 〈 70 head/cluster, the development of it population increased with the increase of basal amount of density, which had great threat to yield. [ Conclusion] The result provided theoretical basis for establishment and improvement of monitoring early warning system, cost-ef- fective control of occurrence and damage of rice brown planthopper, and improvement of the sustained control ability against rice brown planthopper.展开更多
基金Supported by Key Scientific Research Project of Sichuan Provincial Department of Education "Cultivation Physiology and Technique of Plateau Japonica Rice"(10ZA067)
文摘[ Objective ] The study aimed to screen rational seedling raising date and seedling raising mode for plateau japonica rice, in order to reduce the effect of low temperature on seedlings. [ Method ] The seedlings of conventional japonica rice Hexi 22-2 and giant embryo No. 1 japonica rice were treated at day tempera- ture 12℃/night temperature 8℃ and day temperature 14 ℃/night temperature 10 ℃, and the indicators including chlorophyll content, soluble sugar content, sol- uble protein content, survival rate, plant height, number of ≥ 1 cm roots, leaf wilting degree and aboveground dry matter weight were determined after treated for 0, 3 and 6 d, respectively. [ Result] Chlorophyll content of seedlings decreased when treated by low temperature for a certain period; with the prolongation of treat- ment time, contents of soluble sugar and soluble protein in some low temperature treatments increased, while plant height, number of ≥ 1 cm roots and aboveground dry matter weight increased slowly, but the growth rate was significantly lower than that at room temperature; with the decrease of temperature and the prolongation of treatment time, leaf wilting degree increased. The comprehensive experimental results showed that with the increasing duration of low temperature, when two japonica rice varieties grew under the same low temperature, seedlings grew more and more slowly, probably because the lower the treatment temperature, the greater the damage on plants. Giant Embryo No. 1 had weaker cold tolerance than Hexi 22-2. [ Conclusion] The study provides a theoretical basis for formula- tion of seedling raising technique of plateau japonica rice.
基金Supported by Program in 12th Five-year Plan Rural Areas National Science and Technology Plan(2013BAD20B04)Tackle Key in Science and Technology of Chinese Science and Technology Ministry(2011BAD35B02-01)Program in Sience and Technology of Chinese Science and Technology Ministry(2011BAD16B11)
文摘In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.
文摘[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of rice brown planthoppor were released to super indiea and japonica hybrid flee Yongyou 6 during fillering, booting and gain filling stage, respectively. The reproductive rate and variation dynamics of rice brown planthoppor in super rice was also investigated and analyzed. [Result] Tillering stage: the population quantity of rice brown planthoppor during booting and gain filling stage gradually increased with the increasing basal amount of its population during tillering stage; when the population density of rice brown planthopper during grain filling stage reached a certain limit ( 〉 250 head/cluster), the population density during milking stage showed decrease trend with the increasing of such density; the development of rice brown planthoppor population in super rice Yongyou 6 showed the characteristics of fast in middle and slow in two ends. Booting stage: the development of rice brown planthopper pop- ulation from booting to gain filling stage increased with the increasing basal amount of density, and their correlation during milking stage was not significant; flee brown planthopper population still had high reproduction capacity in the middle and late stage of super rice, which had latent risk on rice yield. Gain filling stage : when population density of rice brown planthopper 〈 70 head/cluster, the development of it population increased with the increase of basal amount of density, which had great threat to yield. [ Conclusion] The result provided theoretical basis for establishment and improvement of monitoring early warning system, cost-ef- fective control of occurrence and damage of rice brown planthopper, and improvement of the sustained control ability against rice brown planthopper.