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如东互花米草盐沼中花鲈(Lateolabrax japonicus)仔稚幼鱼的时空分布
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作者 陈渊戈 欧阳珑玲 +10 位作者 范瑞良 李楠楠 吉红九 赵永超 陈玉生 赵永磊 姜伟行 李多 王亦我 陈淑吟 全为民 《渔业信息与战略》 2024年第3期221-228,共8页
互花米草(Spartina alterniflora)的入侵严重影响了盐沼潮滩生态系统的多样性和稳定性。作为中国沿海重要的经济物种,花鲈(Lateolabrax japonicus)是盐沼潮滩生境的常见种。为更好地了解花鲈是否能利用互花米草入侵后的潮滩,2023年3月-2... 互花米草(Spartina alterniflora)的入侵严重影响了盐沼潮滩生态系统的多样性和稳定性。作为中国沿海重要的经济物种,花鲈(Lateolabrax japonicus)是盐沼潮滩生境的常见种。为更好地了解花鲈是否能利用互花米草入侵后的潮滩,2023年3月-2024年2月,针对互花米草入侵后的潮滩内潮沟、光滩、米草盐沼和人工生态低洼塘4种生境开展了连续调查。结果表明,全年共捕获花鲈2 419尾,数量峰值出现在5月,总生物量为18 273.20 g,生物量峰值出现在6月。潮沟和人工生态低洼塘中捕获到的仔稚鱼量相近,但后者的幼鱼单位捕捞努力量渔获量明显低于前者。由此可见,随着花鲈的生长发育,其游泳能力加强,对生境的选择也表现出更高的自主性。在4种生境中,花鲈利用的优先度依次为潮沟>人工生态低洼塘>光滩?米草盐沼,表明互花米草的入侵严重损害了盐沼生境对花鲈早期个体的保育功能,但通过人为营造斑块型鱼类栖息地可部分恢复其这一能力。 展开更多
关键词 花鲈(Lateolabrax japonicus) 互花米草(Spartina alterniflora) 保育 盐沼湿地 生境改良
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A New Furospirostanol Saponin,Ophiofurospiside B from Ophiopogon japonicus(Thunb.) Ker-Gawl 被引量:1
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作者 XU Tun-hai XU Ya-juan +6 位作者 CHEN Ping HAN Dong ZHAO Hong-feng SI Yun-shan XU Dong-ming LI Yu NIU Jian-Zhao 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2007年第6期742-744,共3页
Introduction The tuber of Ophiopogon japonicus ( L. f) Ker- Gawl, which is a Chinese traditional medicine named "maidong" have been recorded to have various medical functions for curing cardiovascular diseases an... Introduction The tuber of Ophiopogon japonicus ( L. f) Ker- Gawl, which is a Chinese traditional medicine named "maidong" have been recorded to have various medical functions for curing cardiovascular diseases and bacterial infections, especially heart diseases. Phytochemical studies on this plant were reported previously. In search of new and bioactive components from Chinese traditional medicines, the tubers of Ophiopogon japonicus have been investigated in this study. The present paper article covers the isolation and structure elucidation of a new furospirostanol saponin, namely, Ophiofurospiside B (compound 1 ) and two known steroidal saponins ( compounds 2 and 3). 展开更多
关键词 Ophiopogon japonicus (L. f) Ker-Gawl Furospirostanol saponin Ophiofurospiside B
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唐山市刺参(Apostichopus japonicus)养殖情况和主要病害防治
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作者 王志丽 岳强 +2 位作者 刘志强 王广宇 刘建朝 《河北渔业》 2024年第6期31-36,共6页
刺参(Apostichopus japonicus)是唐山市重要的水产养殖品种,2022年养殖面积已超过5100 hm2,年产量超过7700 t。本文基于唐山地区多年的刺参苗种繁育和成参养殖的实践经验,总结了几种唐山地区常见的高温、细菌性疾病、霉菌病、寄生虫病... 刺参(Apostichopus japonicus)是唐山市重要的水产养殖品种,2022年养殖面积已超过5100 hm2,年产量超过7700 t。本文基于唐山地区多年的刺参苗种繁育和成参养殖的实践经验,总结了几种唐山地区常见的高温、细菌性疾病、霉菌病、寄生虫病的预防和治疗措施。 展开更多
关键词 唐山市 刺参(Apostichopus japonicus) 病害 防治
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Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system
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作者 Gangyu Sun Yiqian Ju +6 位作者 Cuiping Zhang Lulu Li Xinqiang Jiang Xiaoman Xie Yizeng Lu Kuiling Wang Wei Li 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期252-258,共7页
VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in St... VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene’s knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions. 展开更多
关键词 Styrax japonicus VIGS Reference gene
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Anti-melanogenic properties of FBCC-EP850 derived from Carex pumila Thunb
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作者 Mirissa Hewage Dumindu Kavinda Jinkuk Park +2 位作者 Nayeong Kim Yung Hyun Choi Gi-Young Kim 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第11期477-485,共9页
Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro as... Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro assay.Among them,FBCC-EP850 exhibited the most promising inhibitory activity.Further analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish larvae.Results:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/mL.FBCC-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish larvae.Treatment with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma cells.Additionally,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish larvae.Its anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling pathway.Conclusions:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders. 展开更多
关键词 Carex pumila thunb. MELANOGENESIS TYROSINASE cAMP-CREB-MITF signaling
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Structural Characterization of Chloroplast Genome in Alpinia japonica(Thunb.)Miq.,a Medicinal Plant of the Genus Alpinia
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作者 Wentao Sheng Xi Lei +1 位作者 Xinjie Chen Quan Kuang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第8期1897-1911,共15页
The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were perform... The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A.japonica.The total chloroplast genome length of A.japonica was 161,906 bp,with a typical circular tetrameric structure.And 133 genes were annotated,comprising 87 protein-coding,38 tRNA,and 8 rRNA genes.Furthermore,22 genes contained two copies,and 18 genes owned introns.Repeat sequence analysis showed that it contains 321 simple sequence repeats(SSRs)and 37 long segment repeats.Compared with the chloroplast genomes of eight representative plants in the genus Alpinia,the gene structure,type,and quantity were relatively conservative.Rps12 was the highest variation site in the entire chloroplast gene.A phylogenetic tree showed that the genus Alpinia was the most closely related to the genus Amomum.Meanwhile,A.japonica is the most closely related to Alpinia chinensis belonging to the genus Alpinia.Overall,the chloroplast genome of a new species was reported in the genus Alpinia,and a basis was provided for the utilization of Alpinia plants as a medical resource. 展开更多
关键词 The genus Alpinia Alpinia japonica(thunb.)Miq CHLOROPLAST genome phylogenetic tree
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Proteomic Analysis of Stichopus japonicus and Evaluation of the Antiaging Properties of Its Peptide
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作者 Le WANG Jiao CHEN +4 位作者 Jiali WENG Jinxing FU Wenqin SU Bingmiao GAO Qin CHEN 《Agricultural Biotechnology》 2024年第4期31-37,共7页
The sea cucumber Stichopus japonicus is one of the"Eight Treasures of Seafood"and contains a number of bioactive components involved in multiple physiological and pharmacological functions.Proteins and pepti... The sea cucumber Stichopus japonicus is one of the"Eight Treasures of Seafood"and contains a number of bioactive components involved in multiple physiological and pharmacological functions.Proteins and peptides are generally considered to be responsible for these beneficial properties.In this study,a total of 3478 proteins and 17390 peptides were identified in Stichopus japonicus by proteomics methods.Among them,4 proteins were involved in 8 metabolic pathways,especially oxidative phosphorylation and cell senescence.Subsequently,lifespan assay and oxidative stress test were performed to investigate the peptides prepared from sea cucumber protein hydrolyzate using the aging model of Caenorhabditis elegans.The results of the anti-aging experiment demonstrated that high-dose peptides significantly prolonged the lifespan of nematodes(30.50%),and improved their capacity to inhibit oxidative stress.The results provide evidence supporting the development of bioactive proteins and peptides derived from Stichopus japonicus as functional foods and lay the foundation for the research of an anti-aging drug. 展开更多
关键词 Stichopus japonicus Proteomics Protein extraction Caenorhabditis elegans ANTI-AGING
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忍冬(L.japonica Thunb.)和钻叶紫菀(Aster subulatus Michx.)对较低浓度镉污染的超富集特征比较研究
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作者 李甲萌 张雅坤 +2 位作者 陈波 王安琪 任宗明 《山东师范大学学报(自然科学版)》 2024年第3期259-264,共6页
合适的超富集植物选择对于尾矿重金属污染修复至关重要。针对安丘市石埠子镇废弃矿坑镉(Cd)污染实际,结合忍冬(L.japonica Thunb.)和钻叶紫菀(Aster subulatus Michx.)对重金属Cd的超富集特征比较,分析了30天较低浓度Cd暴露(0.1 mg/kg,0... 合适的超富集植物选择对于尾矿重金属污染修复至关重要。针对安丘市石埠子镇废弃矿坑镉(Cd)污染实际,结合忍冬(L.japonica Thunb.)和钻叶紫菀(Aster subulatus Michx.)对重金属Cd的超富集特征比较,分析了30天较低浓度Cd暴露(0.1 mg/kg,0.3 mg/kg,0.9 mg/kg)下两种超富集植物的毒性响应和超富集特征。研究表明,两种植物的株高和干重均受到明显影响(P<0.05),但忍冬对Cd的平均TF和BCF(TF_(叶)=0.494,TF_(茎)=0.357,BCF_(叶)=12.014,BCF_(茎)=8.689,BCF_(根)=24.424)明显高于钻叶紫菀的平均TF和BCF(TF_(茎)=0.127,TF_(叶)=0.205,BCF_(叶)=3.903,BCF_(茎)=2.412,BCF_(根)=19.189)(P<0.05)。这一研究成果对于废弃矿坑较低浓度Cd污染的超富集生态修复植物种类的选择,构建基于超富集植物的尾矿(渣)原位生态修复技术建设具有重要指导意义。 展开更多
关键词 忍冬 钻叶紫苑 超富集植物 富集能力 生态修复
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海洋酵母培育仿刺参Apostichopus japonicus浮游幼体研究 被引量:8
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作者 赵玉明 毛玉泽 《中国农业科技导报》 CAS CSCD 2009年第3期71-75,共5页
采用海洋酵母(Rhodotorula sp.)作为单细胞藻类的替代饵料培育仿刺参(Apostichopus japonicus)浮游幼体。从生长速度(G)、浮游期成活率(SRP)、变态成活率(SRM)、特定生长率(SGR)和40%樽形幼体出现时间(T)等5个指标比较了... 采用海洋酵母(Rhodotorula sp.)作为单细胞藻类的替代饵料培育仿刺参(Apostichopus japonicus)浮游幼体。从生长速度(G)、浮游期成活率(SRP)、变态成活率(SRM)、特定生长率(SGR)和40%樽形幼体出现时间(T)等5个指标比较了海洋酵母组(Z9)与不同种类和比例单胞藻混合饵料组(8个组合,Z1~Z8)的投喂效果;并确定了不同发育阶段海洋酵母的最佳投喂组合。结果表明:海洋酵母能够促进刺参幼体的正常发育和变态。ANOVA分析表明,不同饵料组间的生长和变态率等指标差异显著,Z2和Z9两组间差异不显著,但显著优于其他单胞藻混合组。投喂不同数量的海洋酵母对浮游幼体也有显著影响。 展开更多
关键词 海洋酵母 仿刺参Apostichopus japonicus 幼体 生长 变态
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多因子对日本囊对虾Marsupenaeus japonicus氮磷代谢的影响 被引量:2
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作者 孙成波 李婷 +1 位作者 李义军 王平 《中山大学学报(自然科学版)》 CAS CSCD 北大核心 2012年第2期91-96,共6页
对日本囊对虾Marsupenaeus japonicus在不同条件下的无机磷、亚硝酸氮、硝酸氮、氨氮的代谢率进行了测定。结果表明:温度、体质量、盐度、pH值和摄食状态对其代谢存在影响。无机磷、硝酸氮、氨氮的代谢率与体质量呈负相关,而亚硝酸氮的... 对日本囊对虾Marsupenaeus japonicus在不同条件下的无机磷、亚硝酸氮、硝酸氮、氨氮的代谢率进行了测定。结果表明:温度、体质量、盐度、pH值和摄食状态对其代谢存在影响。无机磷、硝酸氮、氨氮的代谢率与体质量呈负相关,而亚硝酸氮的代谢率与体质量随体质量的上升而增大;在20~30℃的温度范围内,随着温度的上升,日本囊对虾磷、亚硝酸氮、硝酸氮、氨氮的代谢率均上升;在10‰~31‰的盐度范围内,随着盐度的升高,日本囊对虾(6.739±0.023)g磷、亚硝酸氮的代谢率升高,当盐度为31‰盐度时,磷代谢率达到(0.736±0.002)μg.g-.1h-1,亚硝酸氮代谢率为(0.0778±0.011)μg.g-.1h-1,而硝酸氮、氨氮的代谢率与盐度呈负相关,当盐度为31‰时,硝酸氮的代谢率降为(0.257±0.207)μg.g-.1h-1,氨氮代谢率降为(4.445±0.259)μg.g-.1h-1;在7.5~9.0的pH值范围内,随着pH值的升高,日本囊对虾(6.749±0.013)g磷的代谢率明显下降,氨氮、硝酸氮的代谢率提高,亚硝酸氮的代谢率在pH值为8.5时达到最大值,后又呈下降趋势;日本囊对虾(6.729±0.028)g摄食配合饲料,饱食状态下磷、亚硝酸氮、硝酸氮、氨氮的代谢率比饥饿状态下分别提高了272.02%、91.67%、795.38%、98.54%。 展开更多
关键词 日本囊对虾Marsupenaeus japonicus 氮代谢 磷代谢
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Chemical Constituents of Roots of Ranunculus ternatus Thunb. 被引量:18
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作者 胡小燕 窦德强 +1 位作者 裴玉萍 付文卫 《Journal of Chinese Pharmaceutical Sciences》 CAS 2006年第2期127-129,共3页
Ranunculus ternatus is culus, which is distributed a species of genus Ranunmainly in Henan, Hubei, Guangxi, Sichuan, and Yunnan provinces in China. The root of Ranunculus ternatus has been used for the treatment of fa... Ranunculus ternatus is culus, which is distributed a species of genus Ranunmainly in Henan, Hubei, Guangxi, Sichuan, and Yunnan provinces in China. The root of Ranunculus ternatus has been used for the treatment of faucitis, tuberculosis, neck scrofula, etc in traditional Chinese medicine. Pharmacological experiment indicated that the water extract of Ranunculus ternatus inhibits not only Staphylococcus aureus and pseudomonas aeruginosa, but also S-180, and S37 remarkablely. 展开更多
关键词 Ranuncalus ternatus thunb. γ-keto-δ-valerolactone URIDINE
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GENETIC DIVERSITY IN THE WILD POPULATION AND HATCHERY STOCK OF Penaeus japonicus SHRIMP BY ISOENZYME ANALYSIS 被引量:16
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作者 庄志猛 孟宪红 +1 位作者 权洁霞 戴继勋 《Zoological Research》 CAS CSCD 2000年第4期323-326,共4页
关键词 Genetic diversity Penaeus japonicus ISOENZYME Polyacrylamide gel electrophoresis
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不同时期大叶苦丁茶(Ilex latifolia Thunb.)不同器官中咖啡酰奎宁酸类成分的毛细管电泳分析(英文) 被引量:3
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作者 林春松 徐夙侠 黄青云 《植物研究》 CAS CSCD 北大核心 2018年第3期453-459,共7页
大叶苦丁茶(Ilex latifolia Thunb.)含有丰富的咖啡酰奎宁酸。在本研究中,运用胶束电动毛细管色谱法,测定了在不同发育时期的大叶苦丁茶不同器官中的咖啡酰奎宁酸含量。大叶苦丁茶的不同器官在不同发育时期的咖啡酰奎宁酸含量有着显著... 大叶苦丁茶(Ilex latifolia Thunb.)含有丰富的咖啡酰奎宁酸。在本研究中,运用胶束电动毛细管色谱法,测定了在不同发育时期的大叶苦丁茶不同器官中的咖啡酰奎宁酸含量。大叶苦丁茶的不同器官在不同发育时期的咖啡酰奎宁酸含量有着显著的变化。在叶片中,10天叶龄的叶的咖啡酰奎宁酸含量是19.78 g/100 g,而当叶龄达到12月时,其中的咖啡酰奎宁酸含量已经降为1.41 g/100 g。随着发育时间的增长,雌花、雄花中的含量变化也有类似的变化。雌花(果)和雄花在发育期为10天时的咖啡酰奎宁酸含量高:分别20.29 g/100 g和18.06 g/100g,随后在雌花发育成的7个月果实中下降到0.16 g/100 g,而在40天期接近脱落的雄花中其含量降为10.36 g/100 g。大叶苦丁茶的器官中,除了嫩叶,雄花由于高含量的咖啡酰奎宁酸和较多的生物量,也是一种好的咖啡酰奎宁酸来源材料。本文首次报道了大叶苦丁茶的花中含有大量的咖啡酰奎宁酸。MEKC方法也是首次被用于苦丁茶中的咖啡酰奎宁酸类成分的测定,使用这种方法,大叶苦丁茶中的6种咖啡酰奎宁酸成分在40分钟内完全分离。相较于以前文献中报道的高效液相色谱法,本文为苦丁茶中的咖啡酰奎宁酸的质量控制提供了一种新的检测方法。 展开更多
关键词 咖啡酰奎宁酸衍生物 大叶苦丁茶(Ilex LATIFOLIA thunb.) MEKC 年龄 叶片
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Analysis of Volatile Constituents in Lonicera japonica Thunb. from Different Origins by GC-MS 被引量:11
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作者 杜成智 冯旭 +2 位作者 王卉 吴玲 李盼 《Agricultural Science & Technology》 CAS 2015年第5期1081-1083,1087,共4页
[Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS ... [Objective] This study aimed to analyze the volatile constituents in Lonicera japonica Thunb. from different origins. [Method] HP-5MS capillary columns were used and column temperature was controlled by a program. MS analysis was performed with EI and quadruple mass analyzer. The volatile constituents in L. japonica Thunb. were identified by NIST02 and Wiley275 libraries, and their relative contents were determined with chromatographic peak area normalization method. [Result] According to GC-MS total ion-current chromatograms, 35 volatile constituents were identified in L. japonica Thunb. from Guangxi Zhuang Autonomous Region, mainly including methyl linolenate, n-hexadecanoic acid and ζ-muurolene; 18 volatile constituents were identified in L. japonica Thunb. from Hunan Province, mainly including n-hexadecanoic acid, linoleic acid and α-curcumene. [Conclusion] Main volatile constituents in L. japonica Thunb. from two different origins varied significantly. 展开更多
关键词 Lonicera japonica thunb. Volatile constituents GC-MS Different origins
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Study on the Bacteriostatic Effects of 7 Kinds of Chinese Herbal Medicines such as Ophiopogon japonicus and Comb 被引量:4
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作者 王玮 姜海烽 +5 位作者 邬智伟 钱炯 汪小迪 许锦秀 付浩 傅兰英 《Agricultural Science & Technology》 CAS 2016年第11期2560-2563,共4页
[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogo... [Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug's effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety. 展开更多
关键词 Ophiopogon japonicus HONEYCOMB Panax pseudoginseng Allium macrostemon Juncus effusus Xanthium sibiricum Bacteriostatic effects
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盐度对刺参(Apostichopus japonicus)呼吸和排泄的影响 被引量:66
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作者 袁秀堂 杨红生 +3 位作者 周毅 毛玉泽 张涛 刘鹰 《海洋与湖沼》 CAS CSCD 北大核心 2006年第4期348-354,共7页
采用封闭式呼吸器法研究了(15±0.5)℃水温条件下不同盐度(22、27、31.5、36)对四种规格刺参{S[(39.60±8.77)g]、M[(71.80±14.04)g]、L[(128.30±19.69)g]和XL[(196.65±19.81)g]}呼吸和排泄的影响。结果表明,各... 采用封闭式呼吸器法研究了(15±0.5)℃水温条件下不同盐度(22、27、31.5、36)对四种规格刺参{S[(39.60±8.77)g]、M[(71.80±14.04)g]、L[(128.30±19.69)g]和XL[(196.65±19.81)g]}呼吸和排泄的影响。结果表明,各规格组刺参单位体重耗氧率[Rwr,μg/(g·h)]和排氨率[(Rwe,μg/(g·h)]在盐度22—31.5范围内均随着盐度的升高而降低,当盐度升高至36时,二者都明显升高;各盐度下实验刺参的单位体重耗氧率分别为17.52、16.32、15.49和17.60μg/(g·h),单位体重排氨率分别为1.05、0.88、0.87和0.95μg/(g·h);在同一盐度下,刺参体重越大,其单位个体耗氧率[Rir,μg/(ind·h)]和排氨率[Rie,μg/(ind·h)]越高,二者呈幂函数关系,可用关系式Rir(或Rie)=aWb表示;关系式Rir=aWb中,a值的变动范围为40.6656—81.1900,b值为0.6432—0.8145;而关系式Rie=aWb中,a值的变动范围为2.0947—4.8489,b值为0.6507—0.8072;盐度对刺参O∶N比的影响不显著,各盐度条件下,不同规格的刺参,其O∶N比均在15左右,表明本实验条件下该参代谢所需要的能量主要由脂肪和碳水化合物提供。从呼吸和排泄的角度来看,刺参在其最适温度(15℃)条件下,具有一定的渗透压调节能力,能够适应较广的盐度变化范围(22—36)。 展开更多
关键词 刺参 盐度 体重 呼吸 排泄
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New Steroidal Glycosides from Ophiopogon japonicus 被引量:3
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作者 戴好富 周俊 +1 位作者 谭宁华 丁中涛 《Acta Botanica Sinica》 CSCD 2001年第1期97-100,共4页
Two new C 27 steroidal glycosides, named ophiopojaponin A (1) and B (2), together with two known ones, were isolated from the tubers of the famous traditional Chinese herb Ophiopogon japonicus Ker_Gawl. The spect... Two new C 27 steroidal glycosides, named ophiopojaponin A (1) and B (2), together with two known ones, were isolated from the tubers of the famous traditional Chinese herb Ophiopogon japonicus Ker_Gawl. The spectroscopic and chemical evidence revealed their structures to be pennogenin 3_O_[2′_O_acetyl_α_L_rhamnopyranosyl (1→2)]_β_D_xylopyranosyl (1→3)_β_D_glucopyranoside (1), 26_O_β_D_glucopyranosyl_(22ξ, 25R)_3β, 14α, 22ξ, 26_tetrahydroxyfurost_5_ene 3_O_α_L_rhamnopyranosyl (1→2)_β_D_glucopyranoside (2), diosgenin 3_O_[α_L_rhamnopyranosyl (1→2)]_β_D_xylopyrano_syl (1→3)_β_D_glucopyranoside (3) and ruscogenin 1_O_[α_L_rhamnopyranosyl (1→2)]_β_D_xylopyranosyl (1→3)_β_D_fucopyranoside (4). 展开更多
关键词 Ophiopogon japonicus LILIACEAE C 27 steroidal glycosides
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Variation and Cluster Analysis on Leaf Characters from Different Provenance Sources of Polygonum multiflorum Thunb 被引量:2
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作者 韦艳梅 王凌晖 +2 位作者 曹福亮 韦山青 梁耀丹 《Agricultural Science & Technology》 CAS 2010年第6期94-98,共5页
[Objective] The aim was to study the variation of leaf characters from different provenance sources of Polygonum multiflorum Thunb,as well as to carry out cluster analysis on P.multiflorum from different provenance so... [Objective] The aim was to study the variation of leaf characters from different provenance sources of Polygonum multiflorum Thunb,as well as to carry out cluster analysis on P.multiflorum from different provenance sources to provide basis for the classification,identification,breeding and improved variety selection of P.multiflorum.[Method] Leaf shape characters of 31 copies of germplasm resources in the major distribution region of the whole country were determined,and the genetic variation of P.multiflorum leaves from different producing areas was analyzed.[Result] The leaf characters of single plant of the same experimental provenance source of P.multiflorum were relatively stable,the variation was mainly found on the single leaf area,1/2 leaf width,leaf width and other indicators;the variation of each leaf character among different provenance sources was obvious,and the variation was mainly found on the single leaf weight,leaf area,1/2 leaf width,leaf length and other indicators.The correlation analysis of each leaf character in P.multiflorum suggested that the single leaf area and single leaf weight showed extremely significant positive correlation with leaf length,1/2 leaf width,leaf width,leaf thickness and leaf stem length,while the single leaf area and single leaf weight showed significant negative correlation with WWR(leaf width/1/2 leaf width)and LWR(leaf length/1/2 leaf length),in addition,several macroscopic leaf characters such as leaf length,1/2 leaf width,leaf width,leaf stem length showed extremely positive correlation.The main component analysis result suggested that the contribution rate of accumulation variance of the front three main components was up to 97.4%,which could better reflect the comprehensive performance of leaf characters of different provenance sources of P.multiflorum.The cluster analysis showed that the experimental 31 copies of P.multiflorum provenance sources should be divided into three classes,the first class was distributed in the Middle,Western of Guizhou,northwestern of Guangxi and western areas with higher altitude;the second class was distributed in Hunan,Hubei,Sichuan,Guangdong and the most area of Guangxi;the third class was distributed in Anhui,Jiangsu and Henan and Shandong.[Conclusion] Cluster analysis of leaf characters indicated that the kinds of provenance sources which the geographical position was closer could be got together.The study had provided a certain basis for the classification of P.multiflorum. 展开更多
关键词 Polygonum multiflorum thunb Leaf characters VARIATION Cluster analysis
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温度与盐度对刺参(Apostichopus japonicus)生长的影响 被引量:20
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作者 王国利 祝文兴 +1 位作者 李兆智 付荣恕 《山东科学》 CAS 2007年第3期6-9,共4页
采用实验生态学模拟方法,研究了经40d处理后温度与盐度对刺参生长的影响,以特定生长率(SGR)与净增重为参数反映生长状况。结果不同温度与盐度处理对刺参生长的影响均呈先升高后降低趋势。刺参SGR在温度为17℃时达最大值,为2.031±2.... 采用实验生态学模拟方法,研究了经40d处理后温度与盐度对刺参生长的影响,以特定生长率(SGR)与净增重为参数反映生长状况。结果不同温度与盐度处理对刺参生长的影响均呈先升高后降低趋势。刺参SGR在温度为17℃时达最大值,为2.031±2.772%.d-1,体重净增长量达最大值9.247±12.179g;在盐度为30时达最大值3.067±2.069%.d-1,净增长量12.668±12.717g。根据回归曲线得出刺参最快生长温度是16.8℃,盐度是31。 展开更多
关键词 温度 盐度 刺参 生长
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刺参(Apostichopus japonicus)夏眠期间消化道的组织学研究 被引量:11
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作者 李润玲 丁君 +3 位作者 张玉勇 程莹 曹学彬 常亚青 《海洋环境科学》 CAS CSCD 北大核心 2006年第4期15-19,共5页
运用切片和扫描电镜技术对刺参夏眠状态和正常状态的消化道进行了组织学比较研究。结果表明,与正常状态下相比,进入夏眠后刺参消化道发生退化,消化道整体变细,变短。消化道各部分的界限变得不明显。消化道基本结构未发生变化,仍然由粘... 运用切片和扫描电镜技术对刺参夏眠状态和正常状态的消化道进行了组织学比较研究。结果表明,与正常状态下相比,进入夏眠后刺参消化道发生退化,消化道整体变细,变短。消化道各部分的界限变得不明显。消化道基本结构未发生变化,仍然由粘膜层、粘膜下层、肌层和外膜组成。但内表面柱状细胞变矮,皱襞减少,纤毛和微绒毛几乎完全脱落,分泌颗粒减少或消失。粘膜下层结缔组织变得稀疏,细胞数目减少。肌层变薄。外膜外表面的变化不大,但出现增厚的现象。对于不同夏眠阶段刺参消化道的区别有待于进一步研究。 展开更多
关键词 刺参 复眠 消化道 组织学 扫描电镜
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