AIM:To clarify the significance of JC virus(JCV)T-antigen (T-Ag)expression in human gastric cancer. METHODS:We investigated the relationship between T-Ag detected by immunohistochemistry and Epstein- Barr virus(EBV)in...AIM:To clarify the significance of JC virus(JCV)T-antigen (T-Ag)expression in human gastric cancer. METHODS:We investigated the relationship between T-Ag detected by immunohistochemistry and Epstein- Barr virus(EBV)infection,microsatellite instability (MSI),and genetic and epigenetic alterations in gastric cancers.Mutations in the p53,β-catenin,KRAS,BRAF, PIK3CA genes were analyzed by polymerase chain reaction(PCR)-single strand conformation polymorphism and DNA sequencing.Allelic losses were determined by PCR at 7 microsatellite loci.Aberrant DNA methylation was analyzed by MethyLight assay. RESULTS:JCV T-Ag protein expression was found in 49%of 90 gastric cancer tissues.T-Ag positivity was not correlated with clinicopathological characteristics. T-Ag expression was detected in a similar percentage of EBV positive cancers(4 of 9,44%)and EBV negativecancers(35 of 73,48%).T-Ag expression was detected in a significantly lower percentage of MSI-H cancers (14%)than in non MSI-H cancers(55%,P=0.005). T-Ag expression was detected in a significantly higher percentage of cancers with nuclear/cytoplasmic localization of catenin(15 of 21,71%)than in cancers without(42%,P=0.018).p53 mutations were detected in a significantly lower percentage of T-Ag positive cancers(32%)than in T-Ag negative cancers(57%,P= 0.018).T-Ag positive gastric cancers showed a significant increase in the allelic losses and aberrant methylation compared with T-Ag negative gastric cancers(P=0.008 and P=0.003). CONCLUSION:The results suggest that JCV T-Ag is involved in gastric carcinogenesis through multiple mechanisms of genetic and epigenetic alterations.展开更多
Presented here are the Generalized BCS Equations incorporating Fermi Energy for the study of the {Δ, Tc, jc(T)} values of both elemental and composite superconductors (SCs) for all T ≤ Tc, where Δ, Tc and jc(T) den...Presented here are the Generalized BCS Equations incorporating Fermi Energy for the study of the {Δ, Tc, jc(T)} values of both elemental and composite superconductors (SCs) for all T ≤ Tc, where Δ, Tc and jc(T) denote, respectively, one of the gap values, the critical temperature and the T-dependent critical current density. This framework, which extends our earlier study that dealt with the {Δ0, Tc, jc(0)} values of an SC, is also shown to lead to T-dependent values of several other related parameters such as the effective mass of electrons, their number density, critical velocity, Fermi velocity (VF), coherence length and the London penetration depth. The extended framework is applied to the jc(T) data reported by Romijn et al. for superconducting Aluminium strips and is shown not only to provide an alternative to the explanation given by them, but also to some novel features such as the role of the Sommerfeld coefficient γ(T) in the context of jc(T) and the role of VF(T) in the context of a recent finding by Plumb et al. about the superconductivity of Bi-2212.展开更多
基金Supported by Grants-in-Aid for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology of Japan(Yamamoto H,Imai K and Shinomura Y)Grants-in-Aid for Cancer Research from the Ministry of Health,Laborand Welfare of Japan(Yamamoto H)
文摘AIM:To clarify the significance of JC virus(JCV)T-antigen (T-Ag)expression in human gastric cancer. METHODS:We investigated the relationship between T-Ag detected by immunohistochemistry and Epstein- Barr virus(EBV)infection,microsatellite instability (MSI),and genetic and epigenetic alterations in gastric cancers.Mutations in the p53,β-catenin,KRAS,BRAF, PIK3CA genes were analyzed by polymerase chain reaction(PCR)-single strand conformation polymorphism and DNA sequencing.Allelic losses were determined by PCR at 7 microsatellite loci.Aberrant DNA methylation was analyzed by MethyLight assay. RESULTS:JCV T-Ag protein expression was found in 49%of 90 gastric cancer tissues.T-Ag positivity was not correlated with clinicopathological characteristics. T-Ag expression was detected in a similar percentage of EBV positive cancers(4 of 9,44%)and EBV negativecancers(35 of 73,48%).T-Ag expression was detected in a significantly lower percentage of MSI-H cancers (14%)than in non MSI-H cancers(55%,P=0.005). T-Ag expression was detected in a significantly higher percentage of cancers with nuclear/cytoplasmic localization of catenin(15 of 21,71%)than in cancers without(42%,P=0.018).p53 mutations were detected in a significantly lower percentage of T-Ag positive cancers(32%)than in T-Ag negative cancers(57%,P= 0.018).T-Ag positive gastric cancers showed a significant increase in the allelic losses and aberrant methylation compared with T-Ag negative gastric cancers(P=0.008 and P=0.003). CONCLUSION:The results suggest that JCV T-Ag is involved in gastric carcinogenesis through multiple mechanisms of genetic and epigenetic alterations.
文摘Presented here are the Generalized BCS Equations incorporating Fermi Energy for the study of the {Δ, Tc, jc(T)} values of both elemental and composite superconductors (SCs) for all T ≤ Tc, where Δ, Tc and jc(T) denote, respectively, one of the gap values, the critical temperature and the T-dependent critical current density. This framework, which extends our earlier study that dealt with the {Δ0, Tc, jc(0)} values of an SC, is also shown to lead to T-dependent values of several other related parameters such as the effective mass of electrons, their number density, critical velocity, Fermi velocity (VF), coherence length and the London penetration depth. The extended framework is applied to the jc(T) data reported by Romijn et al. for superconducting Aluminium strips and is shown not only to provide an alternative to the explanation given by them, but also to some novel features such as the role of the Sommerfeld coefficient γ(T) in the context of jc(T) and the role of VF(T) in the context of a recent finding by Plumb et al. about the superconductivity of Bi-2212.
