In recent years,mobile Internet technology and location based services have wide application.Application providers and users have accumulated huge amount of trajectory data.While publishing and analyzing user trajecto...In recent years,mobile Internet technology and location based services have wide application.Application providers and users have accumulated huge amount of trajectory data.While publishing and analyzing user trajectory data have brought great convenience for people,the disclosure risks of user privacy caused by the trajectory data publishing are also becoming more and more prominent.Traditional k-anonymous trajectory data publishing technologies cannot effectively protect user privacy against attackers with strong background knowledge.For privacy preserving trajectory data publishing,we propose a differential privacy based(k-Ψ)-anonymity method to defend against re-identification and probabilistic inference attack.The proposed method is divided into two phases:in the first phase,a dummy-based(k-Ψ)-anonymous trajectory data publishing algorithm is given,which improves(k-δ)-anonymity by considering changes of thresholdδon different road segments and constructing an adaptive threshold setΨthat takes into account road network information.In the second phase,Laplace noise regarding distance of anonymous locations under differential privacy is used for trajectory perturbation of the anonymous trajectory dataset outputted by the first phase.Experiments on real road network dataset are performed and the results show that the proposed method improves the trajectory indistinguishability and achieves good data utility in condition of preserving user privacy.展开更多
Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis.Human-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)...Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis.Human-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system diseases.In this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying mechanism.Our results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral hemorrhage.Additionally,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory microenvironment.RNA sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier integrity.Treatment with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these effects.In summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.展开更多
Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may...Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.展开更多
Mobile devices with global positioning capabilities allow users to retrieve points of interest (POI) in their proximity. Due to the nature of spatial queries, location-based service (LBS) needs the user position in or...Mobile devices with global positioning capabilities allow users to retrieve points of interest (POI) in their proximity. Due to the nature of spatial queries, location-based service (LBS) needs the user position in order to process requests. On the other hand, revealing exact user locations to LBS may pinpoint their identities and breach their privacy. Spatial K-anonymity (SKA) exploits the concept of K-anonymity in order to protect the identity of users from location-based attacks. However, existing reciprocal methods rely on a specialized data structure. In contrast, a reciprocal algorithm was proposed using existing spatial index on the user locations. At the same time, an adjusted median splits algorithm was provided. Finally, according to effectiveness (i.e., anonymizing spatial region size) and efficiency (i.e., construction cost), the experimental results verify that the proposed methods have better performance. Moreover, since using employ general-purpose spatial indices, the proposed method supports conventional spatial queries as well.展开更多
基金supported by the Fundamental Research Funds for the Central Universities(No.GK201906009)CERNET Innovation Project(No.NGII20190704)Science and Technology Program of Xi’an City(No.2019216914GXRC005CG006-GXYD5.2).
文摘In recent years,mobile Internet technology and location based services have wide application.Application providers and users have accumulated huge amount of trajectory data.While publishing and analyzing user trajectory data have brought great convenience for people,the disclosure risks of user privacy caused by the trajectory data publishing are also becoming more and more prominent.Traditional k-anonymous trajectory data publishing technologies cannot effectively protect user privacy against attackers with strong background knowledge.For privacy preserving trajectory data publishing,we propose a differential privacy based(k-Ψ)-anonymity method to defend against re-identification and probabilistic inference attack.The proposed method is divided into two phases:in the first phase,a dummy-based(k-Ψ)-anonymous trajectory data publishing algorithm is given,which improves(k-δ)-anonymity by considering changes of thresholdδon different road segments and constructing an adaptive threshold setΨthat takes into account road network information.In the second phase,Laplace noise regarding distance of anonymous locations under differential privacy is used for trajectory perturbation of the anonymous trajectory dataset outputted by the first phase.Experiments on real road network dataset are performed and the results show that the proposed method improves the trajectory indistinguishability and achieves good data utility in condition of preserving user privacy.
基金supported by the National Natural Science Foundation of China,No.8227050826(to PL)Tianjin Science and Technology Bureau Foundation,No.20201194(to PL)Tianjin Graduate Research and Innovation Project,No.2022BKY174(to CW).
文摘Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis.Human-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system diseases.In this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying mechanism.Our results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral hemorrhage.Additionally,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory microenvironment.RNA sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier integrity.Treatment with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these effects.In summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.
基金supported partly by the National Natural Science Foundation of China,No.82071332the Chongqing Natural Science Foundation Joint Fund for Innovation and Development,No.CSTB2023NSCQ-LZX0041 (both to ZG)。
文摘Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.
基金National Natural Science Foundation of China(No.61070032)
文摘Mobile devices with global positioning capabilities allow users to retrieve points of interest (POI) in their proximity. Due to the nature of spatial queries, location-based service (LBS) needs the user position in order to process requests. On the other hand, revealing exact user locations to LBS may pinpoint their identities and breach their privacy. Spatial K-anonymity (SKA) exploits the concept of K-anonymity in order to protect the identity of users from location-based attacks. However, existing reciprocal methods rely on a specialized data structure. In contrast, a reciprocal algorithm was proposed using existing spatial index on the user locations. At the same time, an adjusted median splits algorithm was provided. Finally, according to effectiveness (i.e., anonymizing spatial region size) and efficiency (i.e., construction cost), the experimental results verify that the proposed methods have better performance. Moreover, since using employ general-purpose spatial indices, the proposed method supports conventional spatial queries as well.
