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IKBKE、YAP1和TEAD2在结直肠癌中的表达及临床意义
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作者 舒莉珊 赵洋 +3 位作者 吴宁琪 刘梦梦 吴琼 欧玉荣 《浙江医学》 CAS 2024年第9期943-949,I0006,共8页
目的探讨核因子κb激酶亚基ε的抑制剂(IKBKE)、Yes相关蛋白1(YAP1)和转录增强结构域转录因子2(TEAD2)在结直肠癌(CRC)组织中的表达及其临床意义。方法收集2016年1月至2017年12月在蚌埠医科大学第一附属医院手术切除的142例CRC组织及对... 目的探讨核因子κb激酶亚基ε的抑制剂(IKBKE)、Yes相关蛋白1(YAP1)和转录增强结构域转录因子2(TEAD2)在结直肠癌(CRC)组织中的表达及其临床意义。方法收集2016年1月至2017年12月在蚌埠医科大学第一附属医院手术切除的142例CRC组织及对应癌旁组织,采用免疫组化法检测标本中IKBKE、YAP1和TEAD2的表达情况。分析3种蛋白在CRC组织中表达的相关性,分析蛋白阳性率与患者临床病理参数及预后的关系;绘制Kaplan-Meier生存曲线,比较这些蛋白不同表达情况患者的生存差异。采用Cox回归分析影响患者预后的危险因素。结果CRC组织中IKBKE、YAP1和TEAD2的阳性率均显著高于癌旁组织(65.5%比9.9%,73.9%比14.1%,66.9%比8.5%,均P<0.05)。IKBKE的表达与肿瘤的分化程度、浸润深度、淋巴结转移、肿瘤-淋巴结-远处转移(TNM)分期有关,YAP1和TEAD2的表达均与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期有关。Spearman秩相关分析显示CRC组织中IKBKE与YAP1、TEAD2表达均呈正相关(均P<0.01)。Kaplan-Meier生存分析显示IKBKE、YAP1和TEAD2阳性表达组的总生存率降低。Cox回归分析显示IKBKE、YAP1和TEAD2阳性、肿瘤分化程度高、TNM分期高是CRC患者预后的独立危险因素。结论CRC中IKBKE、YAP1和TEAD2阳性表达与肿瘤的分化程度、TNM分期、转移等因素有关,可能成为CRC治疗的潜在靶点;检测这3个蛋白的表达有助于评估预后。 展开更多
关键词 结直肠癌 核因子κb激酶亚基ε的抑制剂 Yes相关蛋白1 转录增强结构域转录因子2 预后
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NUMERICAL SIMULATION FOR INCOMPRESSIBLE TURBULENT FLOW IN THE IMPELLER OF SEWAGE PUMP 被引量:3
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作者 YangMinguan GuoXiaomei WangChunlin JiaWeidong YuanChunyuan 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2004年第1期28-30,共3页
The 3-D turbulent flow in the impeller of sewage pump is simulated. Thetime-averaged N-S equations and the kappa-epsilon turbulent model is modified. The calculation iscarried out in body-fitted coordinated grid by ap... The 3-D turbulent flow in the impeller of sewage pump is simulated. Thetime-averaged N-S equations and the kappa-epsilon turbulent model is modified. The calculation iscarried out in body-fitted coordinated grid by applying SIMPLE-C algorithm. The calculated velocity,pressure distributions of the turbulent flow in the sewage pump are obtained for the first time,which will be helpful for the optimal design and performance prediction of sewage pumps on the basisof flow field simulation. 展开更多
关键词 Sewage pump Turbulent flow Numerical simulation kappa-epsilon turbulentmodel
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IKBKE在烟气凝集物诱导人支气管上皮细胞恶性转化过程中的作用 被引量:1
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作者 周继红 洪磊 +7 位作者 蒋鹏 袁娜娜 李勤 陈余清 王效静 朱茂祥 杨陟华 李伟 《第三军医大学学报》 CAS CSCD 北大核心 2018年第13期1221-1228,共8页
目的探讨核因子κB抑制蛋白E抗体(inhibitor of nuclear factor kappa-B kinase subunit epsilon,IKBKE)在卷烟烟气凝集物(cigarette smoke condensate,CSC)诱导永生化人支气管上皮细胞BEP2D恶性转化中的作用及其分子机制。方法 CSC慢... 目的探讨核因子κB抑制蛋白E抗体(inhibitor of nuclear factor kappa-B kinase subunit epsilon,IKBKE)在卷烟烟气凝集物(cigarette smoke condensate,CSC)诱导永生化人支气管上皮细胞BEP2D恶性转化中的作用及其分子机制。方法 CSC慢性染毒BEP2D细胞至P70代(0.001支烟/m L),裸鼠皮下成瘤实验观察染毒细胞是否发生恶性转化;采用Western blot检测各代细胞以及CSC急性染毒BEP2D细胞(0.006支烟/mL)4、8、12、24 h和48 h后IKEBE的表达;免疫组化检测烟熏1、3、9个月A/J小鼠外周小气道和瘤体组织IKBKE的表达;慢病毒IKBKE-siRNA感染P70细胞,观察其对细胞增殖、细胞克隆形成和凋亡的影响;观察敲减IKBKE对STAT3信号通路分子表达的影响及STAT3信号通路激活剂IL-6对细胞恶性转化的影响。结果与P0、P50细胞相比,P70细胞具有恶性转化表型;IKBKE显著高表达于P70细胞(P<0.05),亦是CSC诱导的早期分子事件;在烟气暴露A/J小鼠肺肿瘤组织中IKBKE表达显著增高(P<0.01);siRNA沉默IKBKE可显著抑制细胞增殖和细胞克隆形成,诱导细胞凋亡;该作用与抑制STAT3磷酸化有关。结论 IKBKE可能通过调控STAT3通路抑制细胞凋亡,促进其增殖,参与吸烟诱导肺癌发生。 展开更多
关键词 肺癌 核因子κB抑制蛋白E抗体 卷烟烟气凝集物 人支气管上皮细胞
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COMPUTATION OF SUPERSONIC TURBULENT FLOWFIELD WITH TRANSVERSE INJECTION
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作者 孙得川 胡春波 蔡体敏 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2002年第1期107-113,共7页
Three dimensional steady flowfield generated by transverse sonic injection Into a supersonic flow was simulated by solving the Favre-averaged Navier-Stokes equations using the weighted essentially nonoscillatory (WENO... Three dimensional steady flowfield generated by transverse sonic injection Into a supersonic flow was simulated by solving the Favre-averaged Navier-Stokes equations using the weighted essentially nonoscillatory (WENO) schemes and Jones-Launder k-epsilon model. Results indicate that in the upstream of the square injection there exist two main recirculation regions and the primary vortex induces the horseshoe vortex region. In the downstream there is a low pressure region which conduces a pair of helical vortex. 展开更多
关键词 INJECTION N-S equation kappa-epsilon model weighted ENO scheme
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The Importance of Computational Grids in Hydraulic Numerical Models
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作者 Kuang Cuiping Ph. Doctor, Senior Engineer, River and Harbour Engineering Department, Nanjing Hydraulic Research Institute, Nanjing 210024 《China Ocean Engineering》 SCIE EI 1997年第3期335-346,共12页
The importance of computational grids in hydraulic numerical models is studied by numerical simulation of jet flow in a rectangular duct which is linked with a fixed width inlet and a different width outlet using a st... The importance of computational grids in hydraulic numerical models is studied by numerical simulation of jet flow in a rectangular duct which is linked with a fixed width inlet and a different width outlet using a standard k-epsilon turbulence model. The computational results show the numerical solutions may not be reasonable because of the incorrect computational grid and each numerical model bass grid-independent solution. The computational grid has a definitive effect on the accuracy and stability of the computational solution, which must be divided well according to the simulated geometry and physical characters of hydraulic problems. The main guidelines about the formation of computational grid in such aspects as node distribution, smoothness and skewness of grid, have been given. 展开更多
关键词 kappa-epsilon turbulence model grid node distribution smoothness and skewness of grid grid aspect ratio
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THE INTERACTIONS BETWEEN WAVE-CURRENTS AND OFFSHORE STRUCTURES WITH CONSIDERATION OF FLUID VISCOSITY 被引量:1
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作者 万德成 刘应中 缪国平 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 1996年第4期307-322,共16页
Study of the how held around the large scale offshore structures under the action of waves and viscous currents is of primary importance for the scouring estimation and protection in the vicinity of the structures. Bu... Study of the how held around the large scale offshore structures under the action of waves and viscous currents is of primary importance for the scouring estimation and protection in the vicinity of the structures. But very little has been known in its mechanism when the viscous effects is taken into consideration. As a part of the efforts to tackle the problem, a numerical model is presented for the simulation of the how held around a fixed vertical truncated circular cylinder subjected to waves and viscous currents based on the depth-averaged Reynolds equations and depth-averaged k-epsilon turbulence model. Finite difference method with a suitable iteration defect correct method and an artificial open boundary condition are adopted in the numerical process. Numerical results presented relate to the interactions of a pure incident viscous current with Reynolds number Re = 10(5), a pure incident regular sinusoidal wave, and the coexisting of viscous current and wave with a circular cylinder, respectively. Flow fields associated with the hydrodynamic coefficients of the fixed cylinder, as well as corresponding free surface profiles and wave amplitudes, are discussed. The present method is found to be relatively straightforward, computationally effective and numerically stable for treating the problem of interactions among waves, viscous currents and bodies. 展开更多
关键词 interactions among waves viscous currents and bodies depth-averaged Reynolds equations depth-averaged kappa epsilon turbulence model
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