Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the ...Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the rock scallop Crassadoma gigantea, and the effects of temperature stress on the antioxidant enzymes of rock scallop Crassadoma gigantea were studied in order to explore the effects of temperature on keeping-alive without water and antioxidant system of the rock scallop Crassadoma gigantea. The rock scallop Crassadoma gigantea （shell length （39.7± 5） mm） at different temperatures （5℃ and 15℃）, humidity （moisture treatment, not moisturizing treatment） and oxygen （aerobic treatment, not aerobic treatment） in laboratory was studied by the survival analysis and the analysis of factors affecting the effects of the median lethal time （LTso） in order to investigate the effects of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea. Results showed that low temperature, moisture and aerobic treatment can significantly improve the survival rate and the median lethal time of the rock scallop Crassadoma gigantea （P〈0.05）. The effect of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea was most significant, followed by the effect of oxygen, and that of humidity was the most weakest. Interaction between temperature and humidity, temperature and oxygen significant interaction（P〉0.05）, and interaction between humidity and oxygen interaction was not significant （P〈0.05） in the interaction of three factors. It provided an important basis for the determination of the rock scallop Crassadoma gigantea in the seedling transportation time. In the article, the rock scallop Crassadoma gigantea larvae antioxidant enzymes activity was detected, including SOD activity,CAT activity ,T-A℃ activity （superoxide dismutase activity, catalase activity, total antioxidant capacity） changes through raising the temperature from the low temperature （5℃, 10℃, 15℃, 20℃, 25℃）, to explore the change of the antioxidant enzymes activity under the influence of temperature on the rock scallop Crassadoma gigantea larvae. The SODactivity was significantly lower than that of the other groups （P〈0.05） at 5℃, and the CAT activity had no significant difference between groups （p=0.05）, and the T - AOC level was the lowest significantly below 15℃, 25℃ （P〈0.05）.The SOD and CAT activity had no significant difference in the same experimental time （P〉0.05）, and the T - AOC activity temperature was significantly higher than the rest of the group （P=〈0.05） when temperature at 10℃ and 20℃. The SOD activity had no significant difference （P〉0.05）between groups at 25℃, and the CAT activity was significantly higher than 5℃ 10℃, 15℃ group （P〈0.05）. The T-AOC activity was only lower than 15℃ group （P〉0.05）. It indicated that the high temperature had a clear inducing effect on the CAT activity in the rock scallop Crassadoma gigantea larvae, and had a significant inhibitory effect on the T-AOC activity. The low temperature had a significant inhibitory effect on the activity of T-AOC in the tissue fluid of the scallop. The above results showed that the high and low temperature had a significant effect on the antioxidant enzymes activity of the rock scallop Crassadoma gigantea larvae.展开更多
文摘Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the rock scallop Crassadoma gigantea, and the effects of temperature stress on the antioxidant enzymes of rock scallop Crassadoma gigantea were studied in order to explore the effects of temperature on keeping-alive without water and antioxidant system of the rock scallop Crassadoma gigantea. The rock scallop Crassadoma gigantea （shell length （39.7± 5） mm） at different temperatures （5℃ and 15℃）, humidity （moisture treatment, not moisturizing treatment） and oxygen （aerobic treatment, not aerobic treatment） in laboratory was studied by the survival analysis and the analysis of factors affecting the effects of the median lethal time （LTso） in order to investigate the effects of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea. Results showed that low temperature, moisture and aerobic treatment can significantly improve the survival rate and the median lethal time of the rock scallop Crassadoma gigantea （P〈0.05）. The effect of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea was most significant, followed by the effect of oxygen, and that of humidity was the most weakest. Interaction between temperature and humidity, temperature and oxygen significant interaction（P〉0.05）, and interaction between humidity and oxygen interaction was not significant （P〈0.05） in the interaction of three factors. It provided an important basis for the determination of the rock scallop Crassadoma gigantea in the seedling transportation time. In the article, the rock scallop Crassadoma gigantea larvae antioxidant enzymes activity was detected, including SOD activity,CAT activity ,T-A℃ activity （superoxide dismutase activity, catalase activity, total antioxidant capacity） changes through raising the temperature from the low temperature （5℃, 10℃, 15℃, 20℃, 25℃）, to explore the change of the antioxidant enzymes activity under the influence of temperature on the rock scallop Crassadoma gigantea larvae. The SODactivity was significantly lower than that of the other groups （P〈0.05） at 5℃, and the CAT activity had no significant difference between groups （p=0.05）, and the T - AOC level was the lowest significantly below 15℃, 25℃ （P〈0.05）.The SOD and CAT activity had no significant difference in the same experimental time （P〉0.05）, and the T - AOC activity temperature was significantly higher than the rest of the group （P=〈0.05） when temperature at 10℃ and 20℃. The SOD activity had no significant difference （P〉0.05）between groups at 25℃, and the CAT activity was significantly higher than 5℃ 10℃, 15℃ group （P〈0.05）. The T-AOC activity was only lower than 15℃ group （P〉0.05）. It indicated that the high temperature had a clear inducing effect on the CAT activity in the rock scallop Crassadoma gigantea larvae, and had a significant inhibitory effect on the T-AOC activity. The low temperature had a significant inhibitory effect on the activity of T-AOC in the tissue fluid of the scallop. The above results showed that the high and low temperature had a significant effect on the antioxidant enzymes activity of the rock scallop Crassadoma gigantea larvae.
