Effects of tachyplesin on the morphology and ultrastructure of human gastric carcinoma cell line BGC-823

AIM To investigate the morphological andultrastructural changes in the human gastriccarcinoma cell line BGC-823 after being treatedwith tachyplesin.METHODS Tachyplesin was isolated from acidextracts of Chinese horseshoe crab(Tachypleustridentatus)hemocytes.BGC-823 cells and thecells treated with 2.0mg/L tachyplesin wereexamined respectively under light microscope,scanning and transmission electron microscope.RESULTS BGC-823 cells had undergone therestorational alteration in morphology andultrastructure after tachyplesin treatment.Thechanges were as follows:the shape of cells wasunanimous,the volume enlarged and cellsturned to be flat and spread,the nucleo-cytoplasmic ratio lessened and nuclear shapebecame rather regular,the number of nucleolusreduced and its volume lessened,heter-chromatin decreased while euchromatinincreased in nucleus.In the cytoplasm,mitochondria grew in number with consistentstructure relatively,Golgi complex turned to betypical and well-developed,rough endoplasmicreticulum increased and polyribosomedecreased.The microvilli at cellular surfacewere rare and the filopodia reduced whilelamellipodia increased at the cell edge.CONCLUSION Tachyplesin could alter themalignant morphological and ultrastructuralcharacteristics of human gastric carcinoma cellseffectively and have a certain inducing differen-tiation effect on human gastric carcinoma cells.

Venom gland of the ichneumonid Diadromus collaris： morphology, ultrastructure and age-related changes

Females of the solitary parasitoid Diadromus collaris （Insecta： Hymenoptera： Ichneumonidae） lay eggs in the pupae of Plutella xylostella （Lepidoptera： Plutellidae）, and the venom is synchronously injected into hosts. The venom apparatus consists of two glandular tubules terminating in a common reservoir, A ductule connects the reservoir with the sting apparatus, by which the reservoir content enters the latter. Secretory units line the two glandular tubules. All secretory cells belong to dermal gland type Ⅲ. Dermal gland cells in glandular tubules are more abundant and developed than those in the reservoir. There are extensive rough endoplasmic reticulum and electrondense vesicles, and the microvilli are well developed. By the cuticle-lined central funnel secretion products of secretory units reach the reservoir. Moreover, the secretory apparatus undergoes age-related changes. The secretory units in the venom gland are better developed and more vigorous 7 days after eclosion than those 1 day after eclosion; autolytic processes occur 15 days after eclosion, and the tissue of the reservoir is more prostrate 15 day after eclosion than those 1 day after eclosion. The ovipostion peak of this parasitoid, about 3-7 days after eclosion, corresponds with the period when the venom gland is highly developed in the life span of the wasp.

Effects of chlorine dioxide on morphology and ultrastructure of Fusarium sulphureum and its virulence to potato tubers

The inhibitory effect and its virulence of chlorine dioxide(ClO_(2))against dry rot of potato were investigated.Potatoes were treated by ClO_(2),then observed for indoor bioassay,scanning electron microscopy(SEM)and transmission electron microscopy(TEM)were used to observe the morphology and ultrastructure of hyphae,and evaluated the control efficiency of ClO_(2) on potato tuber(LK99)dry rot by F.sulphureum pre-treatment.The results showed that the pathogen of potato dry rot was sensitive to ClO_(2),the virulence of regression of y=5.05+7.308x,EC50 and EC90 were 0.3490 and 0.6261 respectively,the treatment of ClO_(2) could significantly inhibit the spore germination and mycelium growth of F.sulphureum,which was in a concentration-dependent manner,SEM and TEM observed that the morphology and ultrastructure of F.sulphureum hyphae were regularly damaged by ClO_(2),in vivo experiment further indicated that ClO_(2) could effectively control the dry rot of potato tubers with F.sulphureum,and ClO_(2) at the concentration of 0.75 ug/mL could significantly reduce the incidence of potato tuber dry rot and lesion expansion rate.The study showed that ClO_(2) could greatly against the pathogen of F.sulphureum,which could provide a scientific theoretical basis for the safe and efficient application of ClO_(2) in the prevention and control of potato diseases after harvest.

Preliminary Study on Tensile and Impact Properties of Kenaf/Bamboo Fiber Reinforced Epoxy Composites

The application of natural fibers as reinforcement in composite material has increased due to environmental concerns,low cost,degradability and health concerns.The purpose of this study is to identify the best type of bamboo fibers to be used as reinforcement for kenaf(K)/bamboo hybrid composite.There were three types of bamboo fibers evaluated in this study which include bamboo mat(B),bamboo fabric(BF)and bamboo powder(BP).Chemical composition of B,BF,BP and K fibers were analyzed in this study.The effect of different types of bamboo fibers on tensile,impact,and morphological properties were investigated.The B/epoxy composites displayed the highest tensile strength(53.03 MPa)while K/epoxy composite had the highest tensile modulus(4.71 GPa).Scanning electron micrographs of B/epoxy composites displayed better fiber/matrix interfacial bonding in comparison to other studied composites.Results showed that impact strength of BF-based composite was highest(45.70 J/m).In conclusion,the tensile strength of B/epoxy composite is superior to the other bamboo reinforced composites and will be further evaluated in the next study.

ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells

Objective:To estimate electroporation(EP) influence on malignant and normal cells.Methods: Two cell lines including human malignant melanoma(Me-43) and normal human gingival fibroblast(HCFs) were used.EP parameters were the following:230,1000,1 730,2 300 V/cm;30 μ s by 3 impulses for every case.The viability of cells after EP was estimated by MTT assay. The ullrastructural analysis was observed by transmission electron microscope(Zeiss EM 900). Results:In the current study we observed the intracellular effect following EP on Me-43 and HGF cells.At the conditions applied,we did not observe any significant damage of mitochondrial activity in both cell lines treated by EP.Conversely,we showed that EP in some conditions can stimulate cells to proliferation.Some changes induced by EP were only visible in electron microscopy.In fibroblast cells we observed significant changes in lower parameters of EP(230 and 1 000 V/cm).After applying higher electric field intensities(2 300 V/cm) we detected many vacuoles,myelin-like bodies and swallowed endoplasmic reticulum.In melanoma cells such strong pathological modifications after EP were not observed,in comparison with control cells. The ultrastructure of both treated cell lines was changed according to the applied parameters of EP.Conclusions:We can claim that EP conditions are cell line dependent.In terms of the intracellular morphology,human fibroblasts are more sensitive to electric field as compared with melanoma cells.Optimal conditions should be determined for each cell line.Summarizing our study,we can conclude that EP is not an invasive method for human normal and malignant cells. This technique can be safely applied in chemotherapy for delivering drugs into tumor cells.

两种微塑料对生菜生长及生理特性的影响

为探究聚乙烯(PE)和聚丙烯(PP)两种微塑料对生菜生长及生理特性的影响,以生菜(意大利生菜333)为供试材料进行水培试验,设置对照(CK)、3个PE水平(0.1、0.5、1.0 g·L^(-1))、3个PP水平(0.1、0.5、1.0 g·L^(-1))共7个处理,PE和PP粒径均为13μm,测定了生菜生物量、根系形态参数、光合参数、叶片和根系超微结构以及抗氧化能力等相关指标。试验结果表明:微塑料胁迫下,生菜的干质量显著降低了9.6%~65.4%,根体积显著减少了18.3%~50.2%,光合参数先升高了20.5%后降低40.4%,叶绿素a和类胡萝卜素含量也显著降低了26.1%和29.9%。从叶片和根系的超微结构来看,生菜叶片叶绿体及根系细胞膜、细胞壁均遭受到两种微塑料不同程度的破坏。与CK相比,生菜超氧化物歧化酶(SOD)、过氧化物酶(POD),过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性分别增加了3.0~15.0倍、24.8%~164.0%、2.8~12.0倍和3.5~8.1倍,丙二醛(MDA)和抗坏血酸(ASA)含量增加了1.2~18.9倍和7.2%~10.1%。微塑料对生菜叶片和根系细胞有明显毒害作用,其可破坏叶肉细胞叶绿体,导致生菜叶片的光合参数及光合色素含量降低,同时也抑制了根系的生长发育,降低了其生物量。另外,试验表明生菜抗氧化系统对两种微塑料均产生了响应。

膝关节骨性关节炎并内翻畸形股骨髁软骨退行性变观察研究

目的膝关节骨性关节炎并内翻畸形股骨内侧髁软骨退行性变及超微结构观察。方法收集2018年11月至2021年10月行人工全膝关节置换术的55例膝关节骨性关节炎并内翻畸形患者的股骨髁,股骨内髁设为观察组,股骨外髁设为对照组。两组分别进行大体观察、HE染色、番红O-固绿染色及电镜下观察。结果大体观察:观察组股骨髁关节面不光滑,色泽灰暗,部分软骨剥脱有软骨下骨外露;HE染色观察:观察组软骨细胞排列不规整,潮线紊乱,软骨下骨板异常增厚,对照组股骨髁软骨退变程度较轻;番红O-固绿染色观察:观察组软骨层着色强度不均匀,番红染色在成簇状软骨细胞周围深染,软骨表面染色不均,软骨基质及细胞数量减少,软骨层与软骨下骨边界不清;扫描电镜下观察:观察组关节面凹凸不平,存在大量凸起、裂隙,对照组表面较为平整,可见软骨表面有散在均匀的软骨颗粒。结论膝关节骨性关节炎并内翻畸形股骨内髁关节中钙化层和软骨下骨的改变对骨性关节炎发病机制及病情发展起着重要作用。

部分型圆头精子症患者精子超微结构研究

目的:探讨部分型圆头精子患者中圆头精子和正常精子形态与超微结构差异及辅助生殖结局。方法:收集2023年3月至2024年3月山西省妇幼保健院及山西省人民医院生殖中心6例确诊为部分型圆头精子症患者的临床信息及精液样本,对样本行Diff-Quik染色、铀铅染色,在光学显微镜和透射电子显微镜下观察精子形态,对比圆头精子与正常精子的超微结构区别,分析临床治疗结局。结果:光学显微镜下正常精子头部形态规则,为光滑的椭圆形,顶体区域清晰可见,头、尾部衔接流畅;圆头精子头部无顶体结构,呈圆形,可伴有尾部异常;透射电子显微镜下正常精子头部质膜完整,顶体发育正常,尾部轴丝微管排列整齐;圆头精子头部质膜溶解、顶体缺失或小顶体、核质颗粒化、核区巨大空泡,颈部线粒体肿胀、缺失、排列紊乱,尾部“9+2”微管结构排列混乱且缺失。6例部分型圆头精子症患者均接受了卵细胞胞质内单精子注射(ICSI)助孕治疗,其中1例获得妊娠结局。结论:通过形态学分析,对比正常精子与圆头精子的超微结构差异,部分型圆头精子患者其精子细胞器结构存在显著损伤和超微结构异常,可能是导致其不育问题的一个关键因素,ICSI可以帮助部分圆头精子症患者实现生育愿望。

绵羊亚急性瘤胃酸中毒对瘤胃上皮组织形态的影响

旨在观察亚急性瘤胃酸中毒(SARA)前后绵羊瘤胃上皮显微结构与超微结构的变化。选用体况良好的绵羊15只,随机分为2组,即正常(NG)组(n=5)和SARA组(n=10),其中NG组饲喂精粗比为3∶7的全饲料日粮,SARA组饲喂精粗比为7∶3的全饲料日粮,SARA模型组诱导成功后,在SARA组中选择5只绵羊再饲喂精粗比为3∶7的全饲料日粮,饲喂25 d为恢复(RG)组,动物试验结束后进行屠宰并采集瘤胃组织处腹囊和背囊用于石蜡切片与透射电镜观察形态结构变化。组织学观察结果显示,与NG、RG组相比,SARA组瘤胃乳头部分脱落、变细,形态异常,角质化程度严重,黏膜层变薄,背囊乳头宽度与黏膜上皮厚度均显著低于NG组(P<0.05),而角质层厚度却均显著高于NG组与RG组(P<0.05)。透射电镜结果显示,与NG组相比,SARA组瘤胃上皮腹囊与背囊多数棘突层细胞发生坏死,细胞膜破裂导致胞浆内容物丢失,大多数线粒体发生固缩,细胞间桥粒连接较模糊,结构发生损伤,数量减少,细胞器难分辨;与SARA组相比,RG组细胞形态结构正常,线粒体结构完整清晰且数量较多,细胞间桥粒连接模糊,但部分桥粒连接结构完整且数量有所增加。提示:SARA严重影响着瘤胃上皮组织形态结构,可能长期影响瘤胃上皮保护屏障。

艾菊瓢虫口器及其感器的超微形态特征观察

研究昆虫口器的形态是探究昆虫取食机制的重要手段。为明确艾菊瓢虫成虫口器各部分的超微形态,以及口器感器的种类及分布特征,探究植食性瓢虫口器各结构的功能及协调机制,本研究利用扫描电镜对艾菊瓢虫成虫口器各结构的超微形态及口器感器进行详细观察,并与其他食性瓢虫口器形态进行比较。结果表明,艾菊瓢虫的口器是典型的咀嚼式口器,由上唇、上颚、下颚、下唇和舌组成。根据感器的形态、长度及分布共鉴定出7种感器,即刺形感器、锥形感器、毛形感器、栓锥形感器、表皮孔、板形感器以及分枝刷状结构,其中分枝刷状结构在瓢虫中首次报道。艾菊瓢虫口器基本组成及主要形态与其他食性瓢虫种类相似,但在细微形态上均有差别,这些形态差异与取食习性密切相关。

榛实象成虫触角感器类型、分布及超微结构

为了明确榛实象(Curculio dieckamanni)雌雄成虫触角感器类型、数量、分布及形态特征,利用光学显微镜观察榛实象雌雄成虫触角在头部的位置及形态;利用扫描电镜观察榛实象雌雄成虫触角结构特征,触角感器类型、数量、分布及形态特征。结果表明:在榛实象成虫触角上共发现10种类型感器,包括锥形感器、毛形感器、刺形感器、栓锥形感器、镰刀形感器、表皮孔、鳞形感器、孢子囊形感器、腔锥形感器、B9hm氏鬃毛。雌雄成虫触角各类感器总数分别为2392、2131个,雌成虫触角上的各类感器总数显著多于雄成虫(P<0.05)。锥形感器亚型Sb1和Sb2、毛形感器亚型St1和St2、刺形感器亚型Sc2和Sc3、栓锥形感器Ss1和Ss2、镰刀形感器亚型Sf2、表皮孔亚型Cp1在雌雄成虫触角f7节均有分布,刺形感器Sc1在雌雄触角f6、f7节上分布,刺形感器亚型Sc4、Sc6在雌雄触角的梗节、索节f1~f6分布,刺形感器亚型Sc5在雌雄触角的索节f1~f6、棒节f7分布。鳞形感器分布于雌雄触角的柄节、梗节;B9hm氏鬃毛分布于雌雄触角的梗节近柄节处。毛形感器亚型St3、栓锥形感器亚型Ss3、镰刀型感器亚型Sf1、孢子囊形感器1分布于雌性触角的棒节f7。表皮孔亚型Cp2仅分布于雄性触角的棒节f7,腔锥形感器分布于雄性触角的索节f5、f6节。在雌雄触角各节中,棒节f7中分布感器数量最多,雌雄分别1338、1198个;柄节分布感器数量最少,雌雄分别为66、58个。在雌雄触角感器中,刺形感器亚型Sc5分布最多,雌雄分别为477、433个;锥形感器亚型Sb2分布最少,雌雄均为5个。

7种绿化灌木叶片表型及表皮超微形态与滞尘能力的关系

为选择滞尘能力优良的绿化植物,以南宁市大学路分车隔离带栽植的软枝黄蝉(Allamanda cathartica)、金叶假连翘(Duranta erecta‘Golden Leaves’)、黄金榕(Ficus microcarpa‘Golden Leaves’)、朱槿(Hibiscus rosa-sinensis)、龙船花(Ixora chinensis)、台琼海桐(Pittosporum pentandrum var.formosanum)和鹅掌藤(Heptapleurum arboricola)等7种灌木为研究对象,观察植株叶片形态及叶片表皮的超微形态特征,收集和测量单位叶面积滞尘量。结果表明:该路段植物叶片冬季滞尘量多于夏季,尘粒质量中大于PM_(10)的占比多于PM_(10)或PM_(2.5);所研究的种类中,金叶假连翘的滞尘能力最为突出,龙船花和朱瑾滞留大于PM_(10)颗粒物的能力较强,而黄金榕和台琼海桐滞留PM_(10)和PM_(2.5)等细微颗粒的优势较明显;经量化赋值后主成分分析,叶片是否被毛、叶缘类型、一级侧脉数量、侧脉末梢网结状态和叶脉在叶面的分布状态5个指标包含了植物形态特征与滞尘能力关系的大部分信息;叶片上表皮细胞垂周壁凸出和蜡质层饰纹呈颗粒状滞尘量大,呈条纹状的角质层饰纹过粗或者过细、排列整齐的不利于滞尘功能的发挥,下表皮气孔外拱盖外缘的角质层呈多层环状结构的有利于黏附更多尘粒。在滞尘策略上7种植物在叶片宏观和表皮超微形态特征上表现出一定差异性,金叶假连翘的滞尘量大,叶色多样,景观效果突出,值得大力推广;大气颗粒较多且粒径大的环境,推荐选用龙船花和朱瑾,在颗粒物较细等级占优的环境中可多栽植黄金榕和台琼海桐。

榛实象成虫头部、喙、口器感器类型、分布及超微结构

明确榛实象(Curculio dieckamanni)雌雄成虫头部、喙及口器感器类型、数量、分布及形态特征。利用扫描电镜观察榛实象雌雄成虫头部、喙及口器感器类型、数量、分布及形态特征。在榛实象雌雄成虫头部发现有3种类型的感器,分别为鳞形感器、臼窝状感器和表皮孔,雌雄虫头部感器类型不存在雌雄异型现象;其中鳞形感器数量雌虫中显著高于雄虫(P<0.05),臼窝状感器数量在雌雄虫间差异不显著(P>0.05),表皮孔在雌雄虫头部均仅分布有1个。在榛实象雌雄成虫的喙,发现1种感器为腔锥形感器,该感器数量在雌虫中显著高于雄虫(P<0.05)。在榛实象雌雄成虫口器上发现有4种类型的感器,分别为锥形感器、柱形感器、鱼鳍形感器和轴承短锥形感器,榛实象成虫口器感器种类在雌雄间无异型现象,锥形感器、柱形感器、鱼鳍形感器和轴承短锥形感器分布数量在雌雄间相同。该项研究对榛实象成虫头部、喙及口器感器类型、数量、分布及形态特征进行了观察和分析,这些结果为进一步开展榛实象的行为生态学及生理学的研究提供基础依据。

七种校园道路绿化树种叶片形态与滞尘能力

为选择滞尘能力优良的校园绿化树种,以广西大学校园内主要道路上栽植的白兰(Michelia alba)、大花紫薇(Lagerstroemia speciosa)、小叶榕(Ficus concinna)、中国无忧花(Saraca dives)、杧果(Mangifera indica)、扁桃(Mangifera persiciforma)、金桂(Osmanthus fragrans var.thuergii)等7种树木为研究对象,观察测定了叶片形态及表皮的超微特征,以及单位叶面积滞尘量。结果表明:(1)7种绿化树木冬季叶片滞尘量大部分高于夏季;(2)滞尘总量的排序为:小叶榕>金桂>白兰>杧果>大花紫薇>扁桃>中国无忧花;(3)小叶榕和金桂的滞尘能力突出;(4)大花紫薇滞留大于PM10、扁桃滞留小于PM10颗粒物的能力较强,杧果滞留PM10、白兰滞留PM2.5等颗粒有一定优势。综上结果可知,树冠紧凑、叶片较小及具有锯齿、一级侧脉数量较多或较少的植物滞尘量偏大;超微形态中表皮细胞横切面凸出或下凹、气孔大而下陷、角质层饰纹呈密集条纹状且无规则分布等是滞尘能力较强的特征。不同树种叶片形态和表皮超微结构特征不同,表现出不同滞尘性能,可根据环境特点选择绿化树种。

巴布亚企鹅(Pygoscelis papua)精子形态及超微结构观察

为探明巴布亚企鹅精子的形态结构及超微结构特征,试验对5只处于繁殖季节的雄性巴布亚企鹅采用无创的背部按摩法获得新鲜精液,经扫描电镜和透射电镜对精子形态与超微结构分别进行分析。结果表明:利用扫描电镜观察到的巴布亚企鹅精子属于典型的鞭毛精子,头部呈椭球形,长11.40~11.60μm;精子中段较短,长1.83~2.69μm;精子尾部细长,长47.50~59.74μm;精子超微结构显示,精子的头部长而窄,呈椭球形,在中段发现了自噬体,长0.61~0.81μm,呈新月形,精子的尾部细长、呈鞭毛状。同时观察到了9+2微管结构0.30~0.42μm与尾部中心轴平行排列。此研究从试验层面阐明了巴布亚企鹅精子的超微结构,对巴布亚企鹅授精机制的研究、繁殖和种质资源库建设提供了基础,为有效遏制巴布亚企鹅遗传多样性的降低、也为珍稀动物巴布亚企鹅异位保存提供技术支持。

Antifungal Activity of Chitosan on Fusarium sulphureum in Relation to Dry Rot of Potato Tuber

The antifungal activity of chitosan on Fusarium sulphureum in relation to the inhibitory effect on dry rot of potato tuber was investigated. The results showed spore germination and mycelial growth of F. sulphureum were inhibited by chitosan treatment and the inhibitory effect was highly correlated with chitosan concentration used in this study. Morphological changes such as intertwisting hyphal, distortion, and swelling with excessive branching were observed by scanning electron microscopy （SEM） observation. Transmission electron microscopy （TEM） observation further indicated the ultrastructural alterations of hyphae. These changes included abnormal distribution of cytoplasma, non-membraneous inclusion bodies assembling in cytoplasm, considerable thickening of the hyphal cellular walls, and very frequent septation with malformed septa. Application of chitosan at higher concentration caused serious damage to fungal hyphae, including cellular membrane disorganisation, cell wall disruption, and breaking of inner cytoplast. New hyphae （daughter hyphae） inside the collapsed hyphal cells was often detected in the cytoplasm of chitosan-treated hyphae. In vivo tests showed that chitosan treatment at 0.5 or 1% effectively controlled the dry rot of potato tuber inoculated with a spore suspension ofF. sulphureum. However, the chitosan treatment at 1% caused phytotoxicity to potato tuber. This study suggests that the use of chitosan could be a promising handling as a natural fungicide to partially substitute for the synthetic fungicides in potato tuber.

The Gastric Shield and the Underlying Epithelium of Chlamys Farreri:Morphological, Histological and Histochemical Characterizations

The gastric shield and underlying stomach epithelium of Chlymas farreri were examined at the light and ultrastructural levels. The results showed that the gastric shield consisted of two different size lobes joined together by a narrow middle piece, the thicker lobe was shaped like a funnel, but unclosed at the lateral side; the other lobe was irregularly triangular-shaped. The transverse section of the thicker lobe was obviously laminated and gradually decreased in thickness from the peak to the margins of the shield. The underlying epithelium bore numerous about 3μm diameter spherical processes formed by the apical plasmalemma of the epithelial cells becoming blunt pseudopodia. Microvilli and some interspersed cilia were present in the areas among the spherical processes regions where only microvilli existed. Rough endoplasmic reticulum, Golgi apparatus, different-sized electron-dense secretory granules and electron-lucent vacuoles as well as abundant mitochondria were present in the underlying epithelial cells. Fused droplets of the secretion from the underlying epithelial cells formed the gastric shield.

锰胁迫对垂序商陆叶片形态结构及叶绿体超微结构的影响

以超富集植物垂序商陆为实验材料,通过温室水培实验,研究不同锰处理条件下垂序商陆叶片受害症状、叶片形态结构和叶绿体超微结构的变化。当生长介质中锰供应水平为1 000μmol/L时,垂序商陆生长良好,叶片未表现出受害症状,其形态结构和超微结构也没有明显的变化;当锰供应水平为5 000μmol/L时,叶片开始出现褪绿现象,叶肉细胞排列疏松,栅栏组织细胞膨胀,叶绿体数量减少,外膜部分解体,类囊体膨胀,基质片层扭曲,淀粉粒颗粒变小;当锰供应水平为12 000μmol/L时,叶片发生大量的褪绿现象,伤害率高达87.33%,并且伤害率随着锰胁迫时间的延长明显升高,叶肉细胞发生扭曲变形,叶绿体皱缩,外膜解体,类囊体空泡化加剧,基质片层严重扭曲,基粒排列紊乱甚至模糊成絮状,嗜锇颗粒增多。尽管在高锰浓度胁迫条件下植物叶肉细胞及叶绿体超微结构出现一定程度的毒害特征,但垂序商陆仍能完成部分功能,维持其个体的生长,这进一步表明超富集植物垂序商陆具有极强的锰耐性。

茶树及其他山茶属植物花粉形态、超微结构及演化

用光学显微镜、扫描电镜和透射电镜对26份茶组植物及4份其他山东属植物包括红茶组、油茶组共计30份材料进行了花粉形态及其中6份材料的花粉外壁超微结构的观察。结果表明，其他山茶属植物所具有的花粉粒大、隐沟型萌发孔、外壁表面纹饰光滑、穴大、脊平和外壁内层厚度大等特征均比茶组植物原始，其中红山茶、白毛红山茶和落瓣油茶的表面纹饰与较原始的茶组植物广南茶、圆基茶、老黑菜及属大理茶中的巴达大茶树极为相似，均属穴网状纹饰，因此，茶组植物与其他山茶属植物具有一定的亲缘关系。文中还对苦茶的演化位置、较原始的条组植物花粉表面纹饰的类型进行了讨论。

盼生安对人肝癌细胞超微结构的影响

目的:以氟尿嘧啶(5 Fu)作为阳性对照观察了盼生安对BEL 7402肝癌细胞超微结构的影响,为该药物的抑癌作用提供形态学依据。方法:用透射电镜观察经不同剂量药物处理后细胞超微结构的变化。结果:经盼生安处理后,胞浆空泡化,细胞器中线粒体严重空泡变性,其次是高尔基体和粗面内质网的空泡化,核糖体减少。胞内次级溶酶体,多泡小体,自噬体及髓样小体增多。5 Fu对肝癌细胞形态的影响不如盼生安明显。结论:盼生安主要导致显著的线粒体超微结构改变,胞浆空泡化,抑制了肿瘤细胞的能量代谢和蛋白质合成系统而使细胞功能减退,代谢缓慢最终使细胞退变死亡。