In the Ken 71 development block, fluvial facies of the Neogene Guantao Formation and delta facies of the Paleogene Dongying Formation are the main pay beds. It is a multiple oil and water system which is complicated b...In the Ken 71 development block, fluvial facies of the Neogene Guantao Formation and delta facies of the Paleogene Dongying Formation are the main pay beds. It is a multiple oil and water system which is complicated by faults. Characteristics of the block include a dense well network, thin reservoirs, complicated horizontal relationships, and small velocity difference between reservoir and non-reservoir. Therefore, it is difficult to conduct detailed reservoir description for subsequent development project adjustment. We demonstrate a stochastic seismic inversion which aims at detailed reservoir description. It is a technology which utilizes multiple wells, seismic data, and geological calibration and integrates with 3D structural interpretation results to build a 3D multi-fault detailed and constrained geological model. On this basis, we adopted stochastic seismic inversion to conduct a multi-stratum parameters inversion such as impedance and lithology. As a result, thin interbedded strata in the block were well resolved and the results demonstrated the importance of detailed reservoir inversion for oilfield development.展开更多
Mutations in serologically defined colon cancer autoantigen protein 8(SDCCAG8)were first identified in retinal ciliopathy families a decade ago with unknown function.To investigate the pathogenesis of SDCCAG8-associat...Mutations in serologically defined colon cancer autoantigen protein 8(SDCCAG8)were first identified in retinal ciliopathy families a decade ago with unknown function.To investigate the pathogenesis of SDCCAG8-associated retinal ciliopathies in vivo,we employed CRISPR/Cas9-mediated homology-directed recombination(HDR)to generate two knock-in mouse models,Sdccag8^(Y236X/Y236X) and Sdccag8^(E451GfsX467/E451GfsX467),which carry truncating mutations of the mouse Sdccag8,corresponding to mutations that cause Bardet-Biedl syndrome(BBS)and Senior-L?ken syndrome(SLS)(c.696T>G p.Y232X and c.1339-1340ins G p.E447GfsX463)in humans,respectively.The two mutant Sdccag8 knock-in mice faithfully recapitulated human SDCCAG8-associated BBS phenotypes such as rod-cone dystrophy,cystic renal disorder,polydactyly,infertility,and growth retardation,with varied age of onset and severity depending on the hypomorphic strength of the Sdccag8 mutations.To the best of our knowledge,these knock-in mouse lines are the first BBS mouse models to present with the polydactyly phenotype.Major phototransduction protein mislocalization was also observed outside the outer segment after initiation of photoreceptor degeneration.Impaired cilia were observed in the mutant photoreceptors,renal epithelial cells,and mouse embryonic fibroblasts derived from the knock-in mouse embryos,suggesting that SDCCAG8 plays an essential role in ciliogenesis,and cilium defects are a primary driving force of SDCCAG8-associated retinal ciliopathies.展开更多
In this case report we study the dynamics of the SMR band in a subject affected from Facioscapulohumeral Muscular Dystrophy and subjected to Ken Ware Neuro Physics treatment. We use the Generalized Mutual Information ...In this case report we study the dynamics of the SMR band in a subject affected from Facioscapulohumeral Muscular Dystrophy and subjected to Ken Ware Neuro Physics treatment. We use the Generalized Mutual Information (GMI) to analyze in detail the SMR band at rest during the treatment. Brain dynamics responds to a chaotic-deterministic regime with a complex behaviour?that?constantly self-rearranges and self-organizes such dynamics in function of the outside require-ments. We demonstrate that the SMR chaotic dynamics responds directly to such regime and that also decreasing in EEG during muscular activity really increases its ability of self-arrangement and self-organization in brain. The proposed novel method of the GMI is arranged by us so that it may?be used in several cases of clinical interest. In the case of muscular dystrophy here examined,?GMI?enables us to quantify with accuracy the improvement that the subject realizes during such?treatment.展开更多
This is a study on autonomic neuroscience. In a previous paper in?[1], we studied a subject affected from facioscapulohumeral muscular dystrophy before and after Ken Ware treatment (NPT). Using the non linear methodol...This is a study on autonomic neuroscience. In a previous paper in?[1], we studied a subject affected from facioscapulohumeral muscular dystrophy before and after Ken Ware treatment (NPT). Using the non linear methodology of the Generalized Mutual Information (GMI) analysis of Sensory Motor Rhythm, we produced detailed results evidencing that the mentioned NPT treatment involved a net improvement of the patient under his subjective psychological condition, and in particular, under the neurological and sensory motor profile. We quantified with accuracy the improvement that the subject realized during such treatment. Of course, previous studies of several authors?have evidenced that muscular dystrophies are strongly linked to a profound ANS disfunction.Therefore, the aim of the present study was to analyze the ANS of the subject before and after the treatment. We performed analysis in time as well as in frequency domain and by using non linear?methods. The basic result of the paper was that, according to our analysis, the subjects started?with a serious ANS disfunction before the NPT treatment and that a net improvement was obtained after this therapy. All the examined parameters resulted strongly altered before the treatment and all they returned in the normal range after the NPT.展开更多
文摘In the Ken 71 development block, fluvial facies of the Neogene Guantao Formation and delta facies of the Paleogene Dongying Formation are the main pay beds. It is a multiple oil and water system which is complicated by faults. Characteristics of the block include a dense well network, thin reservoirs, complicated horizontal relationships, and small velocity difference between reservoir and non-reservoir. Therefore, it is difficult to conduct detailed reservoir description for subsequent development project adjustment. We demonstrate a stochastic seismic inversion which aims at detailed reservoir description. It is a technology which utilizes multiple wells, seismic data, and geological calibration and integrates with 3D structural interpretation results to build a 3D multi-fault detailed and constrained geological model. On this basis, we adopted stochastic seismic inversion to conduct a multi-stratum parameters inversion such as impedance and lithology. As a result, thin interbedded strata in the block were well resolved and the results demonstrated the importance of detailed reservoir inversion for oilfield development.
基金supported by the Natural Science Foundation of China(81670893,82121003)Science and Technology Department of Sichuan Province(2021JDZH0031)Chinese Academy of Medical Sciences(2019-I2M-5-032)。
文摘Mutations in serologically defined colon cancer autoantigen protein 8(SDCCAG8)were first identified in retinal ciliopathy families a decade ago with unknown function.To investigate the pathogenesis of SDCCAG8-associated retinal ciliopathies in vivo,we employed CRISPR/Cas9-mediated homology-directed recombination(HDR)to generate two knock-in mouse models,Sdccag8^(Y236X/Y236X) and Sdccag8^(E451GfsX467/E451GfsX467),which carry truncating mutations of the mouse Sdccag8,corresponding to mutations that cause Bardet-Biedl syndrome(BBS)and Senior-L?ken syndrome(SLS)(c.696T>G p.Y232X and c.1339-1340ins G p.E447GfsX463)in humans,respectively.The two mutant Sdccag8 knock-in mice faithfully recapitulated human SDCCAG8-associated BBS phenotypes such as rod-cone dystrophy,cystic renal disorder,polydactyly,infertility,and growth retardation,with varied age of onset and severity depending on the hypomorphic strength of the Sdccag8 mutations.To the best of our knowledge,these knock-in mouse lines are the first BBS mouse models to present with the polydactyly phenotype.Major phototransduction protein mislocalization was also observed outside the outer segment after initiation of photoreceptor degeneration.Impaired cilia were observed in the mutant photoreceptors,renal epithelial cells,and mouse embryonic fibroblasts derived from the knock-in mouse embryos,suggesting that SDCCAG8 plays an essential role in ciliogenesis,and cilium defects are a primary driving force of SDCCAG8-associated retinal ciliopathies.
文摘In this case report we study the dynamics of the SMR band in a subject affected from Facioscapulohumeral Muscular Dystrophy and subjected to Ken Ware Neuro Physics treatment. We use the Generalized Mutual Information (GMI) to analyze in detail the SMR band at rest during the treatment. Brain dynamics responds to a chaotic-deterministic regime with a complex behaviour?that?constantly self-rearranges and self-organizes such dynamics in function of the outside require-ments. We demonstrate that the SMR chaotic dynamics responds directly to such regime and that also decreasing in EEG during muscular activity really increases its ability of self-arrangement and self-organization in brain. The proposed novel method of the GMI is arranged by us so that it may?be used in several cases of clinical interest. In the case of muscular dystrophy here examined,?GMI?enables us to quantify with accuracy the improvement that the subject realizes during such?treatment.
文摘This is a study on autonomic neuroscience. In a previous paper in?[1], we studied a subject affected from facioscapulohumeral muscular dystrophy before and after Ken Ware treatment (NPT). Using the non linear methodology of the Generalized Mutual Information (GMI) analysis of Sensory Motor Rhythm, we produced detailed results evidencing that the mentioned NPT treatment involved a net improvement of the patient under his subjective psychological condition, and in particular, under the neurological and sensory motor profile. We quantified with accuracy the improvement that the subject realized during such treatment. Of course, previous studies of several authors?have evidenced that muscular dystrophies are strongly linked to a profound ANS disfunction.Therefore, the aim of the present study was to analyze the ANS of the subject before and after the treatment. We performed analysis in time as well as in frequency domain and by using non linear?methods. The basic result of the paper was that, according to our analysis, the subjects started?with a serious ANS disfunction before the NPT treatment and that a net improvement was obtained after this therapy. All the examined parameters resulted strongly altered before the treatment and all they returned in the normal range after the NPT.
