[Objectives]This study was conducted to investigate the control efficacy of 6% ascorbic acid water aqua on kiwifruit bacterial canker. [Methods]The ascorbic acid water aqua was sprayed onto annual potted kiwifruit see...[Objectives]This study was conducted to investigate the control efficacy of 6% ascorbic acid water aqua on kiwifruit bacterial canker. [Methods]The ascorbic acid water aqua was sprayed onto annual potted kiwifruit seedlings once every 5 d,for three times in total,and Pseudomonas syringae pv. Actinidiae was inoculated by needle punching 7 d later. Scab diameter and number of newly infected scab were investigated to analyze the control efficacy. [Results] Kiwifruit plants were treated with 6% ascorbic acid water aqua,and after 30 d,the 20 mg/L treatment showed an incidence rate of 25. 5%,and the 60 mg/L treatment exhibited an incidence rate only of 12. 1%; and after 60 d,the relative control efficacy reached 90. 7% at the mass concentration of 60 mg/L. [Conclusions] The6% ascorbic acid water aqua weakened the destruction and expansion of the pathogen in plants,effectively improved the resistance in kiwifruit plants,and had certain control efficacy on kiwifruit canker.展开更多
Survey was conducted to investigate the occurrence laws of bacterial canker disease of kiwifruits in Liupanshui area,Guizhou Province,and the pathogens and hazard symptoms of the canker disease were introduced. The re...Survey was conducted to investigate the occurrence laws of bacterial canker disease of kiwifruits in Liupanshui area,Guizhou Province,and the pathogens and hazard symptoms of the canker disease were introduced. The results showed that the factors influencing the occurrence of bacterial canker disease of kiwifruit were identified as inter-species resistance,agronomic traits,meteorological and ecological conditions,cultivation and management techniques and fertilization levels. Techniques for the comprehensive control of kiwi canker disease were stated,and control suggestions were proposed.展开更多
[Objective]The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [Method]Ten kinds of common commercial bactericides preliminarily screened were selected for ind...[Objective]The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [Method]Ten kinds of common commercial bactericides preliminarily screened were selected for indoor antibacterial test using paper plate method and plate streaking method,and six kinds of bactericides with good antibacterial effects were further screened for indoor toxicity test. [Result]Chloramphenicoloethylicin had the best antibacterial effect,with EC50 of 36. 34 mg / L; followed by Xintianxiucai No. 6 and embamycin,with EC50 of 83. 21 and 92. 34 mg / L,respectively. [Conclusion]Chloramphenicoloethylicin,Xintianxiucai No. 6 and embamycin screened in the test had good antibacterial effect against kiwifruit bacterial canker in Wanzhou District,and chloramphenicoloethylicin was the most effective agent. These three agents could be directly applied to control kiwifruit bacterial canker in production,while application time and climate conditions in fields should also be considered during the actual application process. It is suggested that three agents can be used alternatively in order to delay the resistance of plants.展开更多
基金Supported by Fund for Growth of Young Scientific Talents of Guizhou Education Department(QJHKYZ[2016]269)Open Fund of Key Laboratory of Green Pesticide and Bioengineering,Ministry of Education(2016GDGP0101)Fund for Construction of Key Laboratory of Liupanshui Featured Fruit Tree Resource Research and Utilization(5202020170203)
文摘[Objectives]This study was conducted to investigate the control efficacy of 6% ascorbic acid water aqua on kiwifruit bacterial canker. [Methods]The ascorbic acid water aqua was sprayed onto annual potted kiwifruit seedlings once every 5 d,for three times in total,and Pseudomonas syringae pv. Actinidiae was inoculated by needle punching 7 d later. Scab diameter and number of newly infected scab were investigated to analyze the control efficacy. [Results] Kiwifruit plants were treated with 6% ascorbic acid water aqua,and after 30 d,the 20 mg/L treatment showed an incidence rate of 25. 5%,and the 60 mg/L treatment exhibited an incidence rate only of 12. 1%; and after 60 d,the relative control efficacy reached 90. 7% at the mass concentration of 60 mg/L. [Conclusions] The6% ascorbic acid water aqua weakened the destruction and expansion of the pathogen in plants,effectively improved the resistance in kiwifruit plants,and had certain control efficacy on kiwifruit canker.
基金Supported by the Fund Project for the Growth of Young Scientific Talents of the Department of Education of Guizhou Province(Qianjiaoguhe KY[2016]No.269)the Open-end Fund for the Key Laboratory of Green Pesticide and Agricultural Bioengineering of the Ministry of Education,Guizhou University(2016GDGP0101)the Projects for Undergraduates of Liupanshui Teachers Normal University(LPSSYDXS1613)
文摘Survey was conducted to investigate the occurrence laws of bacterial canker disease of kiwifruits in Liupanshui area,Guizhou Province,and the pathogens and hazard symptoms of the canker disease were introduced. The results showed that the factors influencing the occurrence of bacterial canker disease of kiwifruit were identified as inter-species resistance,agronomic traits,meteorological and ecological conditions,cultivation and management techniques and fertilization levels. Techniques for the comprehensive control of kiwi canker disease were stated,and control suggestions were proposed.
基金Supported by Application Development Research Project of Chongqing Municipal Commission of Science and Technology in 2014 "Occurrence Regularity and Integrated Key Control Technologies of Kiwifruit Bacterial Canker"(cstc2014yykfA8011)
文摘[Objective]The paper was to screen the effective agents against kiwifruit bacterial canker in Wanzhou District of China. [Method]Ten kinds of common commercial bactericides preliminarily screened were selected for indoor antibacterial test using paper plate method and plate streaking method,and six kinds of bactericides with good antibacterial effects were further screened for indoor toxicity test. [Result]Chloramphenicoloethylicin had the best antibacterial effect,with EC50 of 36. 34 mg / L; followed by Xintianxiucai No. 6 and embamycin,with EC50 of 83. 21 and 92. 34 mg / L,respectively. [Conclusion]Chloramphenicoloethylicin,Xintianxiucai No. 6 and embamycin screened in the test had good antibacterial effect against kiwifruit bacterial canker in Wanzhou District,and chloramphenicoloethylicin was the most effective agent. These three agents could be directly applied to control kiwifruit bacterial canker in production,while application time and climate conditions in fields should also be considered during the actual application process. It is suggested that three agents can be used alternatively in order to delay the resistance of plants.
文摘对前期筛选得到的猕猴桃溃疡病病原菌丁香假单胞菌亚种(Pseudomonas syringae pv.actinidiae,PSA)拮抗菌进行了鉴定和抗菌物质分离及分析。用革兰氏染色鉴别菌株;将硫酸铵沉淀溶液进行牛津杯抑菌试验,观察抑菌圈的大小;利用Tricine-SDS-PAGE电泳分析硫酸铵沉淀的抑菌物质;然后溶解沉淀进行去离子水透析脱盐,超滤浓缩后用葡聚糖凝胶柱进行分子筛凝胶过滤层析,最后采用高效液相色谱分析其分子量大小。结果发现:可判断有效抑制PSA生长的假单胞菌菌落形态特征为圆形菌落、乳白色、光滑、凸起、边缘整齐、不透明、不运动,为革兰氏阴性菌,菌体呈短杆状;其抑菌物质为细菌素,在40%和60%硫酸铵饱和度下提取效果最明显,抑菌作用最好,细菌素分子量大小为7 k Da左右。为研发细菌素产品提供了新思路,为防治猕猴桃溃疡病提供了理论基础和应用指导。
