The KNOX gene family codes for transcriptional regulators with a variety of functions in plant developmental and physiological processes. In this study, a genome-wide comparative analysis of KNOX genes in Poplar (Popu...The KNOX gene family codes for transcriptional regulators with a variety of functions in plant developmental and physiological processes. In this study, a genome-wide comparative analysis of KNOX genes in Poplar (Populustrichocarpa) and rice (Oryza sativa L. ssp. japonica) was carried out. With comprehensive computational analyses, which take into account the gene structures, phylogeny and conserved motifs, 15 and 13 KNOX genes in Poplar and rice were identified, respectively. These KNOX genes were further divided into 3 groups. The Poplar gene POPTR_0012s04040 and the rice genes LOC_Os03g47042 and LOC_Os03g47022 were classified to a new group of KNOX genes without ahomeobox domain together with KNATM, which were proposed to play potential role in plant development and pluripotency. The identification of KNATM homolog in monocotyledons (rice) provided a strong support for proposing an ancient shuffling of HOMEOBOX gene with MEINOX gene took place in the KNOX phylogeny. Using subcellular location information, GO (gene ontology) and expression profile analysis, KNOX genes in rice and poplar were proposed to function similarly to the members in Arabidopsis. Our observations may lay the foundation for future functional analysis of KNOX genes in rice and poplar to unravel their biological roles in cellular pluripotency.展开更多
Knox’s personal slogan is'evolve always'.That’s why her career has spanned every shape and screen advertising can fit in--from activations to TVCs,tech builds to social media.As the former social media creat...Knox’s personal slogan is'evolve always'.That’s why her career has spanned every shape and screen advertising can fit in--from activations to TVCs,tech builds to social media.As the former social media creative lead at Digitas,she pushed creative boundaries creating campaigns for Disney Studios,Marvel,and Star Wars across the region.Her next big leap:championing creativity at the Alibaba headquarters in Hangzhou,China.As a staff content strategist,her goal is to create a brand voice for globale-commerce platform AliExpress while driving content for the new retailtainment landscape.展开更多
Nucleotide-binding site and leucine-rich repeat(NLR)proteins are activated by detecting pathogen effectors,which in turn trigger host defenses and cell death.Although many NLRs have been identified,the mechanisms resp...Nucleotide-binding site and leucine-rich repeat(NLR)proteins are activated by detecting pathogen effectors,which in turn trigger host defenses and cell death.Although many NLRs have been identified,the mechanisms responsible for NLR-triggered defense responses are still poorly understood.In this study,through a genome-wide association study approach,we identified a novel NLR gene,Blast Resistance Gene 8(BRG8),which confers resistance to rice blast and bacterial blight diseases.BRG8 overexpression and complementation lines exhibit enhanced resistance to both pathogens.Subcellular localization assays showed that BRG8 is localized in both the cytoplasm and the nucleus.Additional evidence revealed that nuclear-localized BRG8 can enhance rice immunity without a hypersensitive response(HR)-like phenotype.We also demonstrated that the coiled-coil domain of BRG8 not only physically interacts with itself but also interacts with the KNOX II protein HOMEOBOX ORYZA SATIVA59(HOS59).Knockout mutants of HOS59 in the BRG8 background show enhanced resistance to Magnaporthe oryzae strain CH171 and Xoo strain CR4,similar to that of the BRG8 background.By contrast,overexpression of HOS59 in the BRG8 background will compromise the HR-like phenotype and resistance response.Further analysis revealed that HOS59 promotes the degradation of BRG8 via the 26S proteasome pathway.Collectively,our study highlights HOS59 as an NLR immune regulator that fine-tunes BRG8-mediated immune responses against pathogens,providing new insights into NLR associations and functions in plant immunity.展开更多
文摘The KNOX gene family codes for transcriptional regulators with a variety of functions in plant developmental and physiological processes. In this study, a genome-wide comparative analysis of KNOX genes in Poplar (Populustrichocarpa) and rice (Oryza sativa L. ssp. japonica) was carried out. With comprehensive computational analyses, which take into account the gene structures, phylogeny and conserved motifs, 15 and 13 KNOX genes in Poplar and rice were identified, respectively. These KNOX genes were further divided into 3 groups. The Poplar gene POPTR_0012s04040 and the rice genes LOC_Os03g47042 and LOC_Os03g47022 were classified to a new group of KNOX genes without ahomeobox domain together with KNATM, which were proposed to play potential role in plant development and pluripotency. The identification of KNATM homolog in monocotyledons (rice) provided a strong support for proposing an ancient shuffling of HOMEOBOX gene with MEINOX gene took place in the KNOX phylogeny. Using subcellular location information, GO (gene ontology) and expression profile analysis, KNOX genes in rice and poplar were proposed to function similarly to the members in Arabidopsis. Our observations may lay the foundation for future functional analysis of KNOX genes in rice and poplar to unravel their biological roles in cellular pluripotency.
文摘Knox’s personal slogan is'evolve always'.That’s why her career has spanned every shape and screen advertising can fit in--from activations to TVCs,tech builds to social media.As the former social media creative lead at Digitas,she pushed creative boundaries creating campaigns for Disney Studios,Marvel,and Star Wars across the region.Her next big leap:championing creativity at the Alibaba headquarters in Hangzhou,China.As a staff content strategist,her goal is to create a brand voice for globale-commerce platform AliExpress while driving content for the new retailtainment landscape.
基金supported by the National Natural Science Foundation of China(32201739)the Natural Science Foundation of Zhejiang Province(LQ22C130006)+2 种基金the National Key Research and Development Program of China(2021YFD1200503)the Key Research and Development Program of Zhejiang Province(2021C02056)the Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP2013-CNRRI).
文摘Nucleotide-binding site and leucine-rich repeat(NLR)proteins are activated by detecting pathogen effectors,which in turn trigger host defenses and cell death.Although many NLRs have been identified,the mechanisms responsible for NLR-triggered defense responses are still poorly understood.In this study,through a genome-wide association study approach,we identified a novel NLR gene,Blast Resistance Gene 8(BRG8),which confers resistance to rice blast and bacterial blight diseases.BRG8 overexpression and complementation lines exhibit enhanced resistance to both pathogens.Subcellular localization assays showed that BRG8 is localized in both the cytoplasm and the nucleus.Additional evidence revealed that nuclear-localized BRG8 can enhance rice immunity without a hypersensitive response(HR)-like phenotype.We also demonstrated that the coiled-coil domain of BRG8 not only physically interacts with itself but also interacts with the KNOX II protein HOMEOBOX ORYZA SATIVA59(HOS59).Knockout mutants of HOS59 in the BRG8 background show enhanced resistance to Magnaporthe oryzae strain CH171 and Xoo strain CR4,similar to that of the BRG8 background.By contrast,overexpression of HOS59 in the BRG8 background will compromise the HR-like phenotype and resistance response.Further analysis revealed that HOS59 promotes the degradation of BRG8 via the 26S proteasome pathway.Collectively,our study highlights HOS59 as an NLR immune regulator that fine-tunes BRG8-mediated immune responses against pathogens,providing new insights into NLR associations and functions in plant immunity.
