In this paper,the main control methods of soft rot of Amorphophallus konjac are reviewed,with a focus on the current research status of using plant growth promoting rhizobacteria for biological control of soft rot of ...In this paper,the main control methods of soft rot of Amorphophallus konjac are reviewed,with a focus on the current research status of using plant growth promoting rhizobacteria for biological control of soft rot of A.konjac,and future research directions are looked forward to.展开更多
Soft rot disease causes heavy loss in konjac production every year, which caused by the genus Pectobacterium has been recognized as a major reason why konjac industry has not boomed in worldwide. However, intercroppin...Soft rot disease causes heavy loss in konjac production every year, which caused by the genus Pectobacterium has been recognized as a major reason why konjac industry has not boomed in worldwide. However, intercropping with economically important trees can effectively control affect soft rot disease epidemics. Consequently, we conducted a rhizosphere bacterial diversity study to assess how intercropping affects soft rot disease using next-generation DNA sequencing. The results demonstrate the Shannon diversity index and Chao 1 index for soil bacteria were relatively steady under intercropping conditions, but changed greatly for the konjac monocrop with the increase in the number of cropping years. Of the 44 bacterial genera with relative abundance ratios of >0.3%, 11 were significantly affected by the duration of continuous cropping and the cultivation mode. Luteolibacter and Bacteroides showed highly significant differences between the monocrop and the intercrop for three continuous years. Pseudomonas was significantly affected by the different cultivation modes, while Myroides was significantly affected by planting age. Intercropping altered the structure and composition of the soil bacterial community, which led to a relative balance of beneficial bacteria, and the relative balance of beneficial bacteria is helpful to reduce the incidence of soft rot.展开更多
Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) whic...Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions.展开更多
[Objectives]This study aimed to evaluate the quality characteristics of Amorphophallus konjac K.Koch and study the tubers of A.konjac K.Koch by infrared spectroscopy and second derivative method.[Methods]Referring to ...[Objectives]This study aimed to evaluate the quality characteristics of Amorphophallus konjac K.Koch and study the tubers of A.konjac K.Koch by infrared spectroscopy and second derivative method.[Methods]Referring to industrial standards of China for agriculture and related documents,the quality characteristics of A.konjac K.Koch were determined;and using potassium bromide tableting method and Fourier transform infrared spectroscopy,the infrared spectrum and second derivative spectrum of A.konjac K.Koch were studied.[Results]The drying rate,total ash content,acid-insoluble ash content,water extract content,ethanol extract content and crude polysaccharide content of A.konjac K.Koch were above 14.00%,4.57%-11.78%,0.04%-4.87%,26.31%-36.98%,2.20%-7.95%and 40.25%-61.18%,respectively.The infrared spectra of A.konjac K.Koch of different origins were relatively close,and the differences were mainly in peak intensity.The peaks at 1642,1322,1241,1154,1022,863,770 and 576 cm-1 were the main specific peaks.The main component was konjac glucomannan.[Conclusions]FTIR technology is simple and quick,and is suitable for quality control and sample identification of A.konjac K.Koch.This provides a scientific basis for the quality control and comprehensive utilization of A.konjac K.Koch cultivated under forest.展开更多
基于1986~2022年Web of Science(WoS)和中国知网(CNKI)数据库中以魔芋软腐病为主题发表的相关文献,利用CNKI和WoS自带分析工具以及文献计量学软件CiteSpace 6.1.R6,分析了魔芋软腐病的研究现状、研究趋势和研究热点,以期为我国魔芋软腐...基于1986~2022年Web of Science(WoS)和中国知网(CNKI)数据库中以魔芋软腐病为主题发表的相关文献,利用CNKI和WoS自带分析工具以及文献计量学软件CiteSpace 6.1.R6,分析了魔芋软腐病的研究现状、研究趋势和研究热点,以期为我国魔芋软腐病研究领域提供理论支撑。结果显示:1986~2022年魔芋软腐病领域国内外发文量总体呈增长趋势,特别是2000~2010年间发文量增长明显,2010年后研究热度不减,总体发文量较稳定。研究领域内发文量较多的作者及机构间有一定的合作关系。国内外研究方向主要集中在魔芋软腐病的病原菌鉴定、抗病品种选育以及病害防治等三大方面。文献计量分析表明魔芋软腐病研究领域仍存在防控效果不明显等问题,优良抗病性品种培育亟待加强,其也是未来一段时间内的研究方向。展开更多
基金Joint Special Project for Basic Research of Local Undergraduate Universities in Yunnan Province(202101BA070001-057)2021 Provincial College Student Innovation and Entrepreneurship Training Program Project in Yunnan Province(202111393018)Science Research Fund Project of Yunnan Provincial Department of Education(2022Y705,2023Y0857,2023Y0859).
文摘In this paper,the main control methods of soft rot of Amorphophallus konjac are reviewed,with a focus on the current research status of using plant growth promoting rhizobacteria for biological control of soft rot of A.konjac,and future research directions are looked forward to.
文摘Soft rot disease causes heavy loss in konjac production every year, which caused by the genus Pectobacterium has been recognized as a major reason why konjac industry has not boomed in worldwide. However, intercropping with economically important trees can effectively control affect soft rot disease epidemics. Consequently, we conducted a rhizosphere bacterial diversity study to assess how intercropping affects soft rot disease using next-generation DNA sequencing. The results demonstrate the Shannon diversity index and Chao 1 index for soil bacteria were relatively steady under intercropping conditions, but changed greatly for the konjac monocrop with the increase in the number of cropping years. Of the 44 bacterial genera with relative abundance ratios of >0.3%, 11 were significantly affected by the duration of continuous cropping and the cultivation mode. Luteolibacter and Bacteroides showed highly significant differences between the monocrop and the intercrop for three continuous years. Pseudomonas was significantly affected by the different cultivation modes, while Myroides was significantly affected by planting age. Intercropping altered the structure and composition of the soil bacterial community, which led to a relative balance of beneficial bacteria, and the relative balance of beneficial bacteria is helpful to reduce the incidence of soft rot.
文摘Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions.
基金Supported by Science and Technology Support Program of Sichuan Province(2016NYZ0036,21ZDYF2206,2019YFS0181).
文摘[Objectives]This study aimed to evaluate the quality characteristics of Amorphophallus konjac K.Koch and study the tubers of A.konjac K.Koch by infrared spectroscopy and second derivative method.[Methods]Referring to industrial standards of China for agriculture and related documents,the quality characteristics of A.konjac K.Koch were determined;and using potassium bromide tableting method and Fourier transform infrared spectroscopy,the infrared spectrum and second derivative spectrum of A.konjac K.Koch were studied.[Results]The drying rate,total ash content,acid-insoluble ash content,water extract content,ethanol extract content and crude polysaccharide content of A.konjac K.Koch were above 14.00%,4.57%-11.78%,0.04%-4.87%,26.31%-36.98%,2.20%-7.95%and 40.25%-61.18%,respectively.The infrared spectra of A.konjac K.Koch of different origins were relatively close,and the differences were mainly in peak intensity.The peaks at 1642,1322,1241,1154,1022,863,770 and 576 cm-1 were the main specific peaks.The main component was konjac glucomannan.[Conclusions]FTIR technology is simple and quick,and is suitable for quality control and sample identification of A.konjac K.Koch.This provides a scientific basis for the quality control and comprehensive utilization of A.konjac K.Koch cultivated under forest.
文摘基于1986~2022年Web of Science(WoS)和中国知网(CNKI)数据库中以魔芋软腐病为主题发表的相关文献,利用CNKI和WoS自带分析工具以及文献计量学软件CiteSpace 6.1.R6,分析了魔芋软腐病的研究现状、研究趋势和研究热点,以期为我国魔芋软腐病研究领域提供理论支撑。结果显示:1986~2022年魔芋软腐病领域国内外发文量总体呈增长趋势,特别是2000~2010年间发文量增长明显,2010年后研究热度不减,总体发文量较稳定。研究领域内发文量较多的作者及机构间有一定的合作关系。国内外研究方向主要集中在魔芋软腐病的病原菌鉴定、抗病品种选育以及病害防治等三大方面。文献计量分析表明魔芋软腐病研究领域仍存在防控效果不明显等问题,优良抗病性品种培育亟待加强,其也是未来一段时间内的研究方向。
