The purpose of this work was to use a nondestructive method for detecting the brownheart of Korla pear to reduce the chance of infection among pears without brownheart.A mechanical impulse method based on vibration te...The purpose of this work was to use a nondestructive method for detecting the brownheart of Korla pear to reduce the chance of infection among pears without brownheart.A mechanical impulse method based on vibration testing system was used to excite the fruits.The consistent acquisition signal indicated that the test is repeatable at the same positions of fruit equator(cheek).A remarkable frequency signal was excited at 9-12 N force by a rubber tipped hammer.The dominant frequency was identified at the maximum response magnitude to assess the internal defect,and the result was not influenced by the distances between the defect borders and the excitation points.The sharp increase of defect mass could significantly affect the dominant frequency.Relationship between the dominant response frequency(fd)and the defect mass percentage(ω)was characterized by an equation fd=410.649e-0.0833ω+261.947 with a good correlation coefficient(R2=0.925).A defect mass of 2.281%was determined as a discrimination threshold.Once the threshold exceeded 2.281%,the defective pear could be classified with a high accuracy rate of 96.7%.This finding would provide guidance for determining the optimal detecting time to the brownheart of Korla pears,according to the specific storage conditions when the vibration frequency spectrum method is deployed.展开更多
Maintenance of green color is the primary indicator of quality in the market evaluation of Korla Xiang pears at present and can generally be achieved through early harvesting and decreasing the storage temperature, bu...Maintenance of green color is the primary indicator of quality in the market evaluation of Korla Xiang pears at present and can generally be achieved through early harvesting and decreasing the storage temperature, but the fruit quality was reduced by early harvesting, and the decreasing storage temperature increased the risk of chilling injury. The objectives of this study were to determine the optimal storage parameters for different storage times and to find ways to preserve the green skin color of pears. Specifically, we analyzed the effects of the ethylene inhibitor, 1-methylcyclopropene (1-MCP), combined with low temperature on quality and maintenance of the green color of Korla Xiang pears during storage. We found that 1-MCP and/or low temperature reduced the loss of green color at 20℃ after being removed from cold storage. In addition, 1-MCP significantly inhibited the decline of titratable acid and ascorbic acid but had no significant effect on fruit firmness and total soluble solids. Low temperature with or without 1-MCP inhibited the release of ethylene, inhibited the decline in the stalk preservation index, inhibited the increase in decay rate and weight loss rate during storage, and inhibited the increase in the core browning index after 225 days of storage. Different storage temperatures had different effects on the quality of Korla Xiang pears. Despite inhibiting ethylene release, a storage temperature of-1.5℃ increased the respiration rate. Storage at -1.5℃ caused core browning eady during storage due to chilling injury, whereas at 2℃ core browning occurred late during storage due to senescence. In late storage, 1-MCP had no significant effect on the maintenance of Korla Xiang pear quality at 2℃. Based on these results, we determined the optimal combinations of low temperature and 1-MCP treatment to maintain pear quality while avoiding chilling injury. For different marketing times, the optimal conditions for storage until New Year's Day (a storage duration of 90 days) are 2℃ or 1-MCP combined with 2℃. For storage until the Spring Festival (a storage duration of 150 days), the optimal conditions are 0℃ or 1-MCP combined with 0℃, and for storage until May (a storage duration of 225 days), the best conditions are 1-MCP combined with -1.5℃.展开更多
It is difficult to control the quality of Korla pear with different degrees of maturity during storage.Here,a method was proposed for predicting the effects of harvest maturity and cold storage time on the quality ind...It is difficult to control the quality of Korla pear with different degrees of maturity during storage.Here,a method was proposed for predicting the effects of harvest maturity and cold storage time on the quality indices(soluble solid content(SSC)and Vitamin C(Vc)content)of Korla pear.The generalized regression neural network(GRNN)and adaptive neuro-fuzzy inference system(ANFIS)were employed to predict the quality changes of Korla fragrant pear fruit during storage.The results demonstrated that during cold storage the SSC in pears with 10%-70%harvest maturity showed continuous increases in the first 90 d of storage and then a slight decline thereafter,while that in pears with 80%and 90%harvest maturity exhibited slow decreases throughout the storage process.With the extension of storage time,the Vc content of pears with 10%-90%harvest maturity showed continuous decreases.The harvest maturity of Korla pear was extremely positively correlated with SSC and Vc content(p<0.01)in a given storage period.Storage time showed an extremely significant negative correlation with the Vc content(p<0.01)at the 40%-90%harvest maturity and an significant negative correlation with the Vc content(p<0.05)at the 10%-30%harvest maturity.At the 10%-70%harvest maturity,storage time showed a significant positive correlation with the SSC(p<0.05).The trained model could well predict the variation trend of quality indices of pear fruit during storage.The ANFIS with the input membership function of gbellmf had the best performance in predicting the SSC(RMSE=0.175;R2=0.98),and that with the input membership function of trimf exhibited the best performance in predicting Vc content(RMSE=0.075;R2=0.99).The research findings can provide reference for predicting the fruit nutritional quality at delivery and decision-making on the storage time of Korla fragrant pear.展开更多
Korla fragrant pear(KFP)with special fragrance is a unique cultivar in Xinjiang,China.In order to explore the biosynthesis molecular mechanism of chlorogenic acid(CGA)in KFP,the samples at different development period...Korla fragrant pear(KFP)with special fragrance is a unique cultivar in Xinjiang,China.In order to explore the biosynthesis molecular mechanism of chlorogenic acid(CGA)in KFP,the samples at different development periods were collected for transcriptome analysis.High performance liquid chromatography analysis showed that CGA contents of KFP at 88,118 and 163 days after full bloom were(20.96±1.84),(12.01±0.91)and(7.16±0.41)mg/100 g,respectively,and decreased with the fruit development.Pears from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean reads.One‘phenylpropanoid biosynthesis’pathway including 57 unigenes,11 PALs,1 PTAL,64CLs,9 C4Hs,25 HCTs and 5 C3’Hs related to CGA biosynthesis was determined.It was found that the expression levels of 11 differentially expressed genes including 1 PAL,2 C4Hs,34CLs and 5 HCTs were consistent with the change of CGA content.Quantitative polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq data.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.展开更多
To study the effects of different concentrations of Abscisic Acid(ABA) on leaf tissue structure of Korla fragrant pear and lay the foundation for the cultivation and regulation of Korla fragrant pear. Using the paraff...To study the effects of different concentrations of Abscisic Acid(ABA) on leaf tissue structure of Korla fragrant pear and lay the foundation for the cultivation and regulation of Korla fragrant pear. Using the paraffin section method, the leaf thickness, the epidermis thickness, the palisade tissue thickness and the sponge tissue thickness were measured; palisade spongy ratio, Cell Tightness(CT) and Cell Looseness(CL) were examined after treatments with exogenous ABA. Leaf thickness, leaf palisade spongy ratio and palisade tissue thickness increased significantly after exogenous ABA treatment compared with CK, while the effect on leaf epidermis was not obvious. The leaf thickness and palisade tissue thickness increased evidently after 70 mg · LABA treatment by 24.89% and 41.10%, respectively. The tissue structures of leaves were more compact, while CL was lower after 70 and 90 mg · LABA treatments. The effects of different concentrations of exogenous ABA could increase the leaf thickness, palisade tissue thickness of Korla fragrant pear and CT, reduced the thickness of spongy tissue and CL. Among those concentrations of exogenous ABA, the effect of spraying 70 mg · Lexogenous ABA was suitable for improving cold resistance of Korla fragrant pear.展开更多
Based on the introduction of the natural and geographical conditions in Bayingolin Mongol Autonomous Prefecture in Xinjiang(Bazhou),development status of Korla Fragrant Pear is introduced from the two aspects of the p...Based on the introduction of the natural and geographical conditions in Bayingolin Mongol Autonomous Prefecture in Xinjiang(Bazhou),development status of Korla Fragrant Pear is introduced from the two aspects of the production status and the storage and processing status of Korla Fragrant Pear.Among them,production status of Korla Fragrant Pear is analyzed from the aspects of the rapid growth of planting area and the stable growth of output.And the storage and processing status of Korla Fragrant Pear is introduced from the aspects of the development status of the storage industry the development status of processing industry,and the status of domestic and foreign marketing.Problems in the industrialization development of Korla Fragrant Pear in Bazhou are analyzed,such as the weak protection of brand and lack of external propaganda,the imperfect benefit affiliating mechanism between leading enterprises and peasant households,and the marketing network of Korla Fragrant Pear and single mode of marketing.Countermeasures for the acceleration of the industrialization development of Korla Fragrant Pear in Bazhou are put forward,such as making great effort at publicity,brand establishment and counterfeit prevention,cultivating leading enterprises,reducing market risk,implementing industrialization development,adopting various marketing forms and actively developing domestic and international markets.展开更多
[Objectives]To investigate the changes in soil organic matter,nitrogen and phosphorus content in the decomposition process of Korla fragrant pear branches by indoor mixed culture.[Methods]The branches of Korla fragran...[Objectives]To investigate the changes in soil organic matter,nitrogen and phosphorus content in the decomposition process of Korla fragrant pear branches by indoor mixed culture.[Methods]The branches of Korla fragrant pear in the orchard were collected and returned to the field for a period of 150 d for indoor mixed culture.[Results]Different ages of Korla fragrant pear branches have different effects on soil nutrient content during the simulated return to field decomposition process.Compared with the control in the same period,the treatment of returning to field reached a significant level(P<0.05).Compared with the control,the average values of organic matter,total nitrogen and available phosphorus content in treatment 1 and treatment 2 increased by 2.16 times and 1.93 times,61%and 59%,5.88 times and 6.88 times,respectively;compared with the control,the average increase performance of the alkaline hydrolysis nitrogen content of the two treatments was basically the same,and the treatment 2 was the best;compared with the control,the average total phosphorus content of treatment 1 and treatment 2 increased but not significantly.[Conclusions]The contents of soil organic matter,nitrogen and phosphorus were all increased during the decomposition of pear branches,and the overall improvement effect of 10-year-old trees was better than that of 5-year-old trees.Returning the pruned branches to the field can provide a reliable theoretical basis for solving the problem of organic fertilizer shortage in orchards,and also can ensure technical support for improving soil fertility and improving the rhizosphere micro-environment of pear trees.展开更多
To understand the distribution of Apple stem pitting virus (ASPV) in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were us...To understand the distribution of Apple stem pitting virus (ASPV) in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cDNA probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV RNA were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U μL^-1 and 0.4 mmol L^-1 for RNasin and dNTPs respectively, 0.1-1.3 U μ L^-1 SuperScript Ⅱ, 0.6- 0.8 μmol L^-1 primer concentration, and above 0.5 U 100 μL^-1 LA Taq DNA polymerase. The suitable annealing temperature in PCR reaction was 60℃ with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.展开更多
新疆阿克苏地区由Valsa mali var.pyri、Valsa sordida引起的腐烂病是制约库尔勒香梨发展的重要病害,为研究库尔勒香梨感染腐烂病后与防御酶活性的变化关系,以库尔勒香梨为试验材料,分别采集同一株树不同发病组织和不同发病程度枝条韧皮...新疆阿克苏地区由Valsa mali var.pyri、Valsa sordida引起的腐烂病是制约库尔勒香梨发展的重要病害,为研究库尔勒香梨感染腐烂病后与防御酶活性的变化关系,以库尔勒香梨为试验材料,分别采集同一株树不同发病组织和不同发病程度枝条韧皮部,研究其抗性相关酶活性变化。结果表明:病害在完全侵入组织后,SOD活性呈下降的趋势,在发病组织,POD、CAT活性最高,PAL在病健交界处活性最高。随着发病程度的增加,SOD活性整体呈先上升后下降的趋势,POD活性呈逐渐上升的趋势,CAT活性先下降后上升,PAL活性先上升后下降,PPO总体呈下降趋势。库尔勒香梨抗性相关酶活性呈现不同变化响应Valsa侵染。在Valsa与库尔勒香梨互作系统中,抗性酶可作为抗病性和检测病害指标,为检测库尔勒香梨腐烂病发病程度提供理论依据。展开更多
To predict the weight-loss ratio of Korla fragrant pears effectively,improve commodity value and study the variation laws of the weight-loss ratio of damaged fragrant pears during storage,this study predicted the weig...To predict the weight-loss ratio of Korla fragrant pears effectively,improve commodity value and study the variation laws of the weight-loss ratio of damaged fragrant pears during storage,this study predicted the weight-loss ratio of fragrant pears by utilizing the generalized regression neural network(GRNN),support vector regression(SVR),partial least squares regression(PLSR)and error back propagation neural network(BPNN).The prediction performances of GRNN,SVR,PLSR and BPNN models were compared comprehensively,and the optimal model was determined.In addition,the optimal prediction model was verified.The results show that weight-loss ratio of fragrant pears increases gradually with the extension of storage time.During storage,the weight-loss ratio of fragrant pears is positively related to the degree of damage.The trained GRNN,SVR,PLSR and BPNN models can be used to predict the weight-loss ratio of fragrant pears.The BPNN model is the most accurate in predicting the weight-loss ratio of damaged fragrant pears(R^(2)=0.9929;RMSE=0.2138).It has also been proved to have good predictive effect in production practice(R^(2)=0.9377,RMSE=0.7138).The research findings can provide references to predict the delivery quality and time of delivery of Korla fragrant pears.展开更多
基金National Natural Science Foundation of China(No.31560476)Specialized Research Fund for the Doctoral Programs of Xinjiang(No.2013BB011).
文摘The purpose of this work was to use a nondestructive method for detecting the brownheart of Korla pear to reduce the chance of infection among pears without brownheart.A mechanical impulse method based on vibration testing system was used to excite the fruits.The consistent acquisition signal indicated that the test is repeatable at the same positions of fruit equator(cheek).A remarkable frequency signal was excited at 9-12 N force by a rubber tipped hammer.The dominant frequency was identified at the maximum response magnitude to assess the internal defect,and the result was not influenced by the distances between the defect borders and the excitation points.The sharp increase of defect mass could significantly affect the dominant frequency.Relationship between the dominant response frequency(fd)and the defect mass percentage(ω)was characterized by an equation fd=410.649e-0.0833ω+261.947 with a good correlation coefficient(R2=0.925).A defect mass of 2.281%was determined as a discrimination threshold.Once the threshold exceeded 2.281%,the defective pear could be classified with a high accuracy rate of 96.7%.This finding would provide guidance for determining the optimal detecting time to the brownheart of Korla pears,according to the specific storage conditions when the vibration frequency spectrum method is deployed.
基金supported by a grant from the National Key R&D Program of China (2016YFD0400903-06)the emarked fund for China Agriculture Research System (CARS-29-19)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2016-RIP-06)
文摘Maintenance of green color is the primary indicator of quality in the market evaluation of Korla Xiang pears at present and can generally be achieved through early harvesting and decreasing the storage temperature, but the fruit quality was reduced by early harvesting, and the decreasing storage temperature increased the risk of chilling injury. The objectives of this study were to determine the optimal storage parameters for different storage times and to find ways to preserve the green skin color of pears. Specifically, we analyzed the effects of the ethylene inhibitor, 1-methylcyclopropene (1-MCP), combined with low temperature on quality and maintenance of the green color of Korla Xiang pears during storage. We found that 1-MCP and/or low temperature reduced the loss of green color at 20℃ after being removed from cold storage. In addition, 1-MCP significantly inhibited the decline of titratable acid and ascorbic acid but had no significant effect on fruit firmness and total soluble solids. Low temperature with or without 1-MCP inhibited the release of ethylene, inhibited the decline in the stalk preservation index, inhibited the increase in decay rate and weight loss rate during storage, and inhibited the increase in the core browning index after 225 days of storage. Different storage temperatures had different effects on the quality of Korla Xiang pears. Despite inhibiting ethylene release, a storage temperature of-1.5℃ increased the respiration rate. Storage at -1.5℃ caused core browning eady during storage due to chilling injury, whereas at 2℃ core browning occurred late during storage due to senescence. In late storage, 1-MCP had no significant effect on the maintenance of Korla Xiang pear quality at 2℃. Based on these results, we determined the optimal combinations of low temperature and 1-MCP treatment to maintain pear quality while avoiding chilling injury. For different marketing times, the optimal conditions for storage until New Year's Day (a storage duration of 90 days) are 2℃ or 1-MCP combined with 2℃. For storage until the Spring Festival (a storage duration of 150 days), the optimal conditions are 0℃ or 1-MCP combined with 0℃, and for storage until May (a storage duration of 225 days), the best conditions are 1-MCP combined with -1.5℃.
基金The authors express their acknowledgment to the Chinese Natural Science Foundation(Grant No.31660475)Innovation and Entrepreneurship Project of the Xinjiang Production and Construction Group Special Commissioner for Science and Technology(Grant No.2019CB037)+4 种基金University President Fund Project(Grant No.TDZKCQ201902)Xinjiang Production&Construction Group Key Laboratory of Agricultural Products Processing in Xinjiang South(Grant No.AP1905)Young and Middle-aged Scientific and Technological Innovation Leaders Project of Xinjiang Production and Construction Group(Grant No.2018CB014)Strong Youth Key Talents of Scientific and Technological Innovation(Grant No.2021CB039)and Research Project of Double Employment Academician Work Funds Opening in Tarim University(Grant No.SPYS202002)for financial support and all of the persons who assisted in this writing.
文摘It is difficult to control the quality of Korla pear with different degrees of maturity during storage.Here,a method was proposed for predicting the effects of harvest maturity and cold storage time on the quality indices(soluble solid content(SSC)and Vitamin C(Vc)content)of Korla pear.The generalized regression neural network(GRNN)and adaptive neuro-fuzzy inference system(ANFIS)were employed to predict the quality changes of Korla fragrant pear fruit during storage.The results demonstrated that during cold storage the SSC in pears with 10%-70%harvest maturity showed continuous increases in the first 90 d of storage and then a slight decline thereafter,while that in pears with 80%and 90%harvest maturity exhibited slow decreases throughout the storage process.With the extension of storage time,the Vc content of pears with 10%-90%harvest maturity showed continuous decreases.The harvest maturity of Korla pear was extremely positively correlated with SSC and Vc content(p<0.01)in a given storage period.Storage time showed an extremely significant negative correlation with the Vc content(p<0.01)at the 40%-90%harvest maturity and an significant negative correlation with the Vc content(p<0.05)at the 10%-30%harvest maturity.At the 10%-70%harvest maturity,storage time showed a significant positive correlation with the SSC(p<0.05).The trained model could well predict the variation trend of quality indices of pear fruit during storage.The ANFIS with the input membership function of gbellmf had the best performance in predicting the SSC(RMSE=0.175;R2=0.98),and that with the input membership function of trimf exhibited the best performance in predicting Vc content(RMSE=0.075;R2=0.99).The research findings can provide reference for predicting the fruit nutritional quality at delivery and decision-making on the storage time of Korla fragrant pear.
基金supported by Major scientific and technological projects of XPCC(2020KWZ-012)。
文摘Korla fragrant pear(KFP)with special fragrance is a unique cultivar in Xinjiang,China.In order to explore the biosynthesis molecular mechanism of chlorogenic acid(CGA)in KFP,the samples at different development periods were collected for transcriptome analysis.High performance liquid chromatography analysis showed that CGA contents of KFP at 88,118 and 163 days after full bloom were(20.96±1.84),(12.01±0.91)and(7.16±0.41)mg/100 g,respectively,and decreased with the fruit development.Pears from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean reads.One‘phenylpropanoid biosynthesis’pathway including 57 unigenes,11 PALs,1 PTAL,64CLs,9 C4Hs,25 HCTs and 5 C3’Hs related to CGA biosynthesis was determined.It was found that the expression levels of 11 differentially expressed genes including 1 PAL,2 C4Hs,34CLs and 5 HCTs were consistent with the change of CGA content.Quantitative polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq data.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.
基金Supported by Science and Technology Planning Projects of China during the 12th Five-year Plan-key Techniques of High Quality and High Efficiency Production of Fruit Trees(2014BAD16B07)Central Finance Forestry Science and Technology Extension Project(ZYLTKJTG2015017)National Featured Fruit Trees Root Stock Germplasm Resources Platform(Luntai)(NICGR2017-60)
文摘To study the effects of different concentrations of Abscisic Acid(ABA) on leaf tissue structure of Korla fragrant pear and lay the foundation for the cultivation and regulation of Korla fragrant pear. Using the paraffin section method, the leaf thickness, the epidermis thickness, the palisade tissue thickness and the sponge tissue thickness were measured; palisade spongy ratio, Cell Tightness(CT) and Cell Looseness(CL) were examined after treatments with exogenous ABA. Leaf thickness, leaf palisade spongy ratio and palisade tissue thickness increased significantly after exogenous ABA treatment compared with CK, while the effect on leaf epidermis was not obvious. The leaf thickness and palisade tissue thickness increased evidently after 70 mg · LABA treatment by 24.89% and 41.10%, respectively. The tissue structures of leaves were more compact, while CL was lower after 70 and 90 mg · LABA treatments. The effects of different concentrations of exogenous ABA could increase the leaf thickness, palisade tissue thickness of Korla fragrant pear and CT, reduced the thickness of spongy tissue and CL. Among those concentrations of exogenous ABA, the effect of spraying 70 mg · Lexogenous ABA was suitable for improving cold resistance of Korla fragrant pear.
文摘Based on the introduction of the natural and geographical conditions in Bayingolin Mongol Autonomous Prefecture in Xinjiang(Bazhou),development status of Korla Fragrant Pear is introduced from the two aspects of the production status and the storage and processing status of Korla Fragrant Pear.Among them,production status of Korla Fragrant Pear is analyzed from the aspects of the rapid growth of planting area and the stable growth of output.And the storage and processing status of Korla Fragrant Pear is introduced from the aspects of the development status of the storage industry the development status of processing industry,and the status of domestic and foreign marketing.Problems in the industrialization development of Korla Fragrant Pear in Bazhou are analyzed,such as the weak protection of brand and lack of external propaganda,the imperfect benefit affiliating mechanism between leading enterprises and peasant households,and the marketing network of Korla Fragrant Pear and single mode of marketing.Countermeasures for the acceleration of the industrialization development of Korla Fragrant Pear in Bazhou are put forward,such as making great effort at publicity,brand establishment and counterfeit prevention,cultivating leading enterprises,reducing market risk,implementing industrialization development,adopting various marketing forms and actively developing domestic and international markets.
基金Supported by Key R&D Program of Hainan Province in 2021(ZDYF2021XDNY152).
文摘[Objectives]To investigate the changes in soil organic matter,nitrogen and phosphorus content in the decomposition process of Korla fragrant pear branches by indoor mixed culture.[Methods]The branches of Korla fragrant pear in the orchard were collected and returned to the field for a period of 150 d for indoor mixed culture.[Results]Different ages of Korla fragrant pear branches have different effects on soil nutrient content during the simulated return to field decomposition process.Compared with the control in the same period,the treatment of returning to field reached a significant level(P<0.05).Compared with the control,the average values of organic matter,total nitrogen and available phosphorus content in treatment 1 and treatment 2 increased by 2.16 times and 1.93 times,61%and 59%,5.88 times and 6.88 times,respectively;compared with the control,the average increase performance of the alkaline hydrolysis nitrogen content of the two treatments was basically the same,and the treatment 2 was the best;compared with the control,the average total phosphorus content of treatment 1 and treatment 2 increased but not significantly.[Conclusions]The contents of soil organic matter,nitrogen and phosphorus were all increased during the decomposition of pear branches,and the overall improvement effect of 10-year-old trees was better than that of 5-year-old trees.Returning the pruned branches to the field can provide a reliable theoretical basis for solving the problem of organic fertilizer shortage in orchards,and also can ensure technical support for improving soil fertility and improving the rhizosphere micro-environment of pear trees.
基金supported by the National Natural Science Foundation of China (30360066)the National Key Technologies R&D Program of China (2003BA546C)the Foundation Science and Technology Commission Xinjiang Production and Construction Corps,China(NKB02SDXNK01SW)
文摘To understand the distribution of Apple stem pitting virus (ASPV) in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cDNA probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV RNA were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U μL^-1 and 0.4 mmol L^-1 for RNasin and dNTPs respectively, 0.1-1.3 U μ L^-1 SuperScript Ⅱ, 0.6- 0.8 μmol L^-1 primer concentration, and above 0.5 U 100 μL^-1 LA Taq DNA polymerase. The suitable annealing temperature in PCR reaction was 60℃ with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.
文摘新疆阿克苏地区由Valsa mali var.pyri、Valsa sordida引起的腐烂病是制约库尔勒香梨发展的重要病害,为研究库尔勒香梨感染腐烂病后与防御酶活性的变化关系,以库尔勒香梨为试验材料,分别采集同一株树不同发病组织和不同发病程度枝条韧皮部,研究其抗性相关酶活性变化。结果表明:病害在完全侵入组织后,SOD活性呈下降的趋势,在发病组织,POD、CAT活性最高,PAL在病健交界处活性最高。随着发病程度的增加,SOD活性整体呈先上升后下降的趋势,POD活性呈逐渐上升的趋势,CAT活性先下降后上升,PAL活性先上升后下降,PPO总体呈下降趋势。库尔勒香梨抗性相关酶活性呈现不同变化响应Valsa侵染。在Valsa与库尔勒香梨互作系统中,抗性酶可作为抗病性和检测病害指标,为检测库尔勒香梨腐烂病发病程度提供理论依据。
基金the Chinese Natural Science Foundation(Grant No.32260618 and 32202139)the Bingtuan Guiding Science and Technology Plan Program(Grant No.2022ZD094)for financial support.
文摘To predict the weight-loss ratio of Korla fragrant pears effectively,improve commodity value and study the variation laws of the weight-loss ratio of damaged fragrant pears during storage,this study predicted the weight-loss ratio of fragrant pears by utilizing the generalized regression neural network(GRNN),support vector regression(SVR),partial least squares regression(PLSR)and error back propagation neural network(BPNN).The prediction performances of GRNN,SVR,PLSR and BPNN models were compared comprehensively,and the optimal model was determined.In addition,the optimal prediction model was verified.The results show that weight-loss ratio of fragrant pears increases gradually with the extension of storage time.During storage,the weight-loss ratio of fragrant pears is positively related to the degree of damage.The trained GRNN,SVR,PLSR and BPNN models can be used to predict the weight-loss ratio of fragrant pears.The BPNN model is the most accurate in predicting the weight-loss ratio of damaged fragrant pears(R^(2)=0.9929;RMSE=0.2138).It has also been proved to have good predictive effect in production practice(R^(2)=0.9377,RMSE=0.7138).The research findings can provide references to predict the delivery quality and time of delivery of Korla fragrant pears.