Anticancer,Anti-inflammatory,Analgesic,and Anxiolytic Effects of Koumine and Their Molecular Mechanisms

Koumine is an indole alkaloid monomer extracted from the Chinese herb Gelsemium elegans,which has a variety of pharmacological effects.This paper provides a comprehensive summary of the pharmacological effects and molecular mechanisms of koumine,with a particular emphasis on its mechanisms of action in the context of anticancer,anti-inflammatory,analgesic,and anxiolytic properties.The aim is to provide a theoretical foundation for further research and the application of koumine in clinical practice.

Studies on the method of total synthesis of koumine——Ⅰ.4-Substituted 1,2-dihydropyridine derivatives and their function as a conjugated diene in Diels-Alder reaction

A route to the total synthesis of koumine has been suggested.Five 4-substituted 1,2- dihydropyridine derivatives,which are supposed to be a conjugated diene of Diels-Alder reaction to produce the isoquinuclidine ring of koumine,have been synthesized.Only two of them were found to be able to undergo the Diels-Alder reaction.The stereochemistry of the reaction product has also been examined.

钩吻生物碱的提取分离及结构鉴定

中草药钩吻中具有抗癌活性生物碱单体成分的提取分离,并测定其结构。方法:药材粉碎后用3%NaOH碱化后,晾干,用氯仿浸泡,回收氯仿提取液得浸膏。加酸溶解后,用氯仿萃取,碱化水层,再用氯仿萃取,得到钩吻总生物碱。然后经硅胶柱层析、反相ODS柱层析以及制备型HPLC层析分离纯化,并经理化性质和光谱学数据的测定鉴定单体化合物的结构。结果:共分离得到12个单体化合物,经测定紫外光谱、1H-NMR数据并与文献报道相比较,初步确定两个化合物的结构分别为gelsemine和koumine。结论:通过硅胶柱层析、ODS柱层析和HPLC相结合的方法可以达到分离钩吻总生物碱的目的。