The random amplified polymorphic DNA marker （RAPD） was applied to detect the genetic relationships and diversity among 21 germplasm materials of Kudingcha species in Oleaceae, which involved 8 species, i.e., Ligustr...The random amplified polymorphic DNA marker （RAPD） was applied to detect the genetic relationships and diversity among 21 germplasm materials of Kudingcha species in Oleaceae, which involved 8 species, i.e., Ligustrum robustum （Roxb.） Blume, L. henryi Hemsl., L. japonicum Thunb, L. japonicum Thunb. var. pubscens Koidz, L. lucidum Ait., L. pedunculare Rehd, Osmanthus masumuranus Hayata, and L. delavayanm Hariot. 20 RAPD primers selected were applied for the amplification on the 21 germplasm materials mentioned above. 427 bands were obtained, and the percentage of polymorphic bands （PPB） was 97.7%. The genetic similarity coefficients （GS） ranged from 0.1522 to 0.8322 with an average of 0.5466. There was a significant genetic difference among germplasm materials of Kudingcha species in Oleaceae, and UPGMA cluster based on the GS of RAPD could distinguish all test germplasm materials clearly and indicated the relationship of the 8 species mentioned above, all of which indicated that RAPD markers could be used for the studies of genetic diversity and relationship and classification of germplasm resources of Kudingcha species in Oleaceae. Analysis results of RAPD showed that L. japonicum Thunb. var. pubscens Koidz has closer genetic relationship with L. pedunculare Rehd and further genetic relationship with L. japonicum Thunb. among all tested species. The authors suggest that further research is needed to study whether L. japonicum Thunb. var. pubscens Koidz should be classified into a variata of L. japonicum Thunb, or should be considered as an independent species. The analysis results supported that L. pururascens Y. C. Yang should be combined into L. robustum （Roxb.） Blume.展开更多
This research the preventive effect of Kudingcha polyphenols(Kp)on colitis based on animal experiments.Experimental mice divided into four groups,including normal group,model group,low-concentration Kp(LKP)group and h...This research the preventive effect of Kudingcha polyphenols(Kp)on colitis based on animal experiments.Experimental mice divided into four groups,including normal group,model group,low-concentration Kp(LKP)group and high-concentration Kp(HKP)group,and they all smeared and given 0.15 mL 1%oxazolone solution by lavage to induce BALB/c mice colitis.DAI,colon weight/length ratio,serum levels of cytokines,related antioxidant activities of colon tissues such,and the mRNA expressions.The experimental results show that KP can significantly(p<0.05)reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis,increase colon weight/length ratio.It can also significantly reduce(p<0.05)the activities of MPO,NO,MDA in mice colon tissues with colitis,increase the activity of GSH,and significantly increase(p<0.05)the level of IL-2 and decrease the level of IL-10 in mice serum with colitis.The results of qPCR assay show that Kp can significantly increase(p<0.05)the expression of Cu/Zn-SOD,Mn-SOD,GSH-Px,CAT,c-Kit,SCF and decrease the expression of IL-8,CXCR2 in mice colon tissues with colitis.Kp has a good preventive effect on colitis,and high concentration of PT has better preventive effect.展开更多
基金supported by the National Natural Science Foundation of China (39860048)the Natural Science Foundation of Guizhou Province in China([2005] 2033)
文摘The random amplified polymorphic DNA marker （RAPD） was applied to detect the genetic relationships and diversity among 21 germplasm materials of Kudingcha species in Oleaceae, which involved 8 species, i.e., Ligustrum robustum （Roxb.） Blume, L. henryi Hemsl., L. japonicum Thunb, L. japonicum Thunb. var. pubscens Koidz, L. lucidum Ait., L. pedunculare Rehd, Osmanthus masumuranus Hayata, and L. delavayanm Hariot. 20 RAPD primers selected were applied for the amplification on the 21 germplasm materials mentioned above. 427 bands were obtained, and the percentage of polymorphic bands （PPB） was 97.7%. The genetic similarity coefficients （GS） ranged from 0.1522 to 0.8322 with an average of 0.5466. There was a significant genetic difference among germplasm materials of Kudingcha species in Oleaceae, and UPGMA cluster based on the GS of RAPD could distinguish all test germplasm materials clearly and indicated the relationship of the 8 species mentioned above, all of which indicated that RAPD markers could be used for the studies of genetic diversity and relationship and classification of germplasm resources of Kudingcha species in Oleaceae. Analysis results of RAPD showed that L. japonicum Thunb. var. pubscens Koidz has closer genetic relationship with L. pedunculare Rehd and further genetic relationship with L. japonicum Thunb. among all tested species. The authors suggest that further research is needed to study whether L. japonicum Thunb. var. pubscens Koidz should be classified into a variata of L. japonicum Thunb, or should be considered as an independent species. The analysis results supported that L. pururascens Y. C. Yang should be combined into L. robustum （Roxb.） Blume.
基金The present research was supported by the Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDX201601040)the Introduction of High-level Personnel Research Start-up Fund of Chongqing University of Education(2013BSRC001),China.
文摘This research the preventive effect of Kudingcha polyphenols(Kp)on colitis based on animal experiments.Experimental mice divided into four groups,including normal group,model group,low-concentration Kp(LKP)group and high-concentration Kp(HKP)group,and they all smeared and given 0.15 mL 1%oxazolone solution by lavage to induce BALB/c mice colitis.DAI,colon weight/length ratio,serum levels of cytokines,related antioxidant activities of colon tissues such,and the mRNA expressions.The experimental results show that KP can significantly(p<0.05)reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis,increase colon weight/length ratio.It can also significantly reduce(p<0.05)the activities of MPO,NO,MDA in mice colon tissues with colitis,increase the activity of GSH,and significantly increase(p<0.05)the level of IL-2 and decrease the level of IL-10 in mice serum with colitis.The results of qPCR assay show that Kp can significantly increase(p<0.05)the expression of Cu/Zn-SOD,Mn-SOD,GSH-Px,CAT,c-Kit,SCF and decrease the expression of IL-8,CXCR2 in mice colon tissues with colitis.Kp has a good preventive effect on colitis,and high concentration of PT has better preventive effect.
