TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal...TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing enzymes.As impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule dynamics.We therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to microtubules.To investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic littermates.In sum,P301L-TAU results in changes in microtubule PTMs,suggestive of impairment of microtubule stability.This is accompanied by missorting and aggregation of TAU in mice but not in i Neurons.Microtubule PTMs/impairment may be of key importance in tauopathies.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact mo...BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact molecular mechanisms leading to the progression of HCC are still unclear.Research has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor tissues.In this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between them.AIM To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC patients.METHODS In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC patients.Furthermore,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,etc.The statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank test.RESULTS In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival rate.ASH1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated region.Furthermore,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via ASH1L.For the exploration mechanism,we found ASH1L may promote an immunosuppressive microenvironment in HCC and ASH1L affects the expression of the cell junction protein zonula occludens-1,which is potentially relevant to the immune system.CONCLUSION Loss function of microRNA-142-3p induces cancer progression and immune evasion through upregulation of ASH1L in HCC.Both microRNA-142-3p and ASH1L can feature as new biomarker for HCC in the future.展开更多
In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order t...In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order to prove the main results,we first give some bidirectional estimates for several typical integrals.展开更多
The QK(p)-Teichmüller space is introduced and studied in this paper.Various characterizations of the QK(p)-Teichmüller space and the QK,0(p)-Teichmüller space are given.Their Schwarzian derivative model...The QK(p)-Teichmüller space is introduced and studied in this paper.Various characterizations of the QK(p)-Teichmüller space and the QK,0(p)-Teichmüller space are given.Their Schwarzian derivative model and pre-logarithmic derivative model are also discussed.展开更多
In this paper, we introduce and study the notion of HB-closed sets in L-topological space. Then, HB-convergence theory for L-molecular nets and L-ideals is established in terms of HB-closedness. Finally, we give a new...In this paper, we introduce and study the notion of HB-closed sets in L-topological space. Then, HB-convergence theory for L-molecular nets and L-ideals is established in terms of HB-closedness. Finally, we give a new definition of fuzzy H-continuous [1] which is called HB-continuity on the basis of the notion of H-bounded L-subsets in L-topological space. Then we give characterizations and properties by making use of HB-converges theory of L-molecular nets and L-ideals.展开更多
[Objective] The study aimed to understand the mutagenic effects of space flight on alfalfa seeds.[Method] Seeds of three lines of alfalfa were carried into orbit by the satellite 'Shijian-8' for space fight,th...[Objective] The study aimed to understand the mutagenic effects of space flight on alfalfa seeds.[Method] Seeds of three lines of alfalfa were carried into orbit by the satellite 'Shijian-8' for space fight,the indices including seed germination rate,plant height and growth rate,were measured after the seeds been retrieved.[Result] Remarkable mutagenic effects occurred on the flight seeds,which were mainly presented by abnormal cotyledon,extended variation range of plant height and growth rate.Nineteen plants with increased plant height were preliminarily screened from the T0 progenies,but whether the mutated trait could inherit should be confirmed in further study.Three flight lines of alfalfa are different in the percentages of abnormal cotyledon(10%-18%),variation range of plant height(increased 30%-150% compared to control),selected plant number with increased plant height(5-7 plants),suggesting that differences exits between the three lines in mutation efficiency.[Conclusion] Valuable mutated materials could be obtained by space flight and applied in modern agriculture.展开更多
In this paper we prove the following Hajek Renyi inequality:Let 0<p≤1 ,then for any Banach space B , any L p integrable B valued random variable sequence {D n,n≥1} ,any real number sequence {b...In this paper we prove the following Hajek Renyi inequality:Let 0<p≤1 ,then for any Banach space B , any L p integrable B valued random variable sequence {D n,n≥1} ,any real number sequence {b n,n≥1} with 0<b n↑∞ ,any integer n≥1 ,there exits a constant C=C p>0 (only depending on p ) such thatP( sup j≥nji=1D ib j≥ε)≤Cε -p (∞j=n+1E‖D j‖ pb p j+nj=1E‖D j‖ pb p n) In the other direction,we prove some strong laws of large numbers and the integrability of the maximal functions for B valued random variable sequences by using this inequality and the Hajeck Renyi inequality we have obtained recently.Some known results are extended and improved.展开更多
In this article, the authors give a typical integral＇s bidirectional estimates for allcases. At the same time, several equivalent characterizations on the F（p, q, s, k） space in theunit ball of Cn are given.
This paper is devoted to characterizing the Riemann-Stieltjes operators and pointwise multipliers on F（p, q, s） spaces in the unit ball of C^n which contain many classical function spaces, such as the Bloch space, B...This paper is devoted to characterizing the Riemann-Stieltjes operators and pointwise multipliers on F（p, q, s） spaces in the unit ball of C^n which contain many classical function spaces, such as the Bloch space, BMOA and Q8 spaces. The boundedness and compactness of these operators on F（p, q, s） spaces are characterized by means of an embedding theorem, i.e., F（p,q, s） spaces boundedly embedded into the tent-type spaces Tp,s^∞（μ）展开更多
LetΩ be a bounded symmetric domain in Cn. The purpose of this article is to define and characterize the general function space F（p, q, s） on Ω. Characterizing functions in the F（p, q, s） space is a work of consi...LetΩ be a bounded symmetric domain in Cn. The purpose of this article is to define and characterize the general function space F（p, q, s） on Ω. Characterizing functions in the F（p, q, s） space is a work of considerable interest nowadays. In this article, the authors give several equivalent descriptions of the functions in the F（p, q, s） space on Ω in terms of fractional differential operators. At the same time, the authors give the relationship between F（p, q, s） space and Bloch type space on Ω too.展开更多
This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to ...This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to Bers-type spaces Hv^∞（ or little Bers-type spaces Hv,o∞ ）, where v is normal.展开更多
This paper deals with the boundedness and compactness of the compositionintegral type operators T g, from F (p, q, s) spaces to(little) Bloch-type spaces in the unit ball of C n , where Tg,φf(z) =∫01fφ(tz)Rg(tz)(dt...This paper deals with the boundedness and compactness of the compositionintegral type operators T g, from F (p, q, s) spaces to(little) Bloch-type spaces in the unit ball of C n , where Tg,φf(z) =∫01fφ(tz)Rg(tz)(dt)/t , z ∈ B, g ∈ H(B) and φ∈H(B, B).展开更多
基金supported by the Koeln Fortune Program/Faculty of Medicine,University of Cologne,the Alzheimer Forschung Initiative e.V.(grant#22039,to HZ)open-access funding from the DFG/GRC issued to the University of CologneAlzheimer Forschung Initiative e.V.for Open Access Publishing(a publication grant#P2401,to MAAK)。
文摘TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the axon.TAU is missorted and aggregated in an array of diseases known as tauopathies.Microtubules are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing enzymes.As impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule dynamics.We therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to microtubules.To investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic littermates.In sum,P301L-TAU results in changes in microtubule PTMs,suggestive of impairment of microtubule stability.This is accompanied by missorting and aggregation of TAU in mice but not in i Neurons.Microtubule PTMs/impairment may be of key importance in tauopathies.
基金Supported by the Haihe Laboratory of Cell Ecosystem Innovation Fund,No.22HHXBJC00001the Key Discipline Special Project of Tianjin Municipal Health Commission,No.TJWJ2022XK016.
文摘BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated mortality.Efficient therapeutic targets are beneficial to treat and predict the disease.Currently,the exact molecular mechanisms leading to the progression of HCC are still unclear.Research has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor tissues.In this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between them.AIM To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC patients.METHODS In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC patients.Furthermore,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,etc.The statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank test.RESULTS In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival rate.ASH1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated region.Furthermore,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via ASH1L.For the exploration mechanism,we found ASH1L may promote an immunosuppressive microenvironment in HCC and ASH1L affects the expression of the cell junction protein zonula occludens-1,which is potentially relevant to the immune system.CONCLUSION Loss function of microRNA-142-3p induces cancer progression and immune evasion through upregulation of ASH1L in HCC.Both microRNA-142-3p and ASH1L can feature as new biomarker for HCC in the future.
基金supported by the Natural Science Foundation of Hunan Province of China(2022JJ30369)the Education Department Important Foundation of Hunan Province in China(23A0095)。
文摘In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order to prove the main results,we first give some bidirectional estimates for several typical integrals.
基金supported by the National Natural Science Foundation of China(12271017)supported by the Natural Science Foundation of Shandong Province(ZR2022MA018)by the National Natural Science Foundation of China(12171221)。
文摘The QK(p)-Teichmüller space is introduced and studied in this paper.Various characterizations of the QK(p)-Teichmüller space and the QK,0(p)-Teichmüller space are given.Their Schwarzian derivative model and pre-logarithmic derivative model are also discussed.
文摘In this paper, we introduce and study the notion of HB-closed sets in L-topological space. Then, HB-convergence theory for L-molecular nets and L-ideals is established in terms of HB-closedness. Finally, we give a new definition of fuzzy H-continuous [1] which is called HB-continuity on the basis of the notion of H-bounded L-subsets in L-topological space. Then we give characterizations and properties by making use of HB-converges theory of L-molecular nets and L-ideals.
基金Supported by Special Fund on Fundamental Scientific Research as Operating Expenses among Commonweal Scientific Research Institutions at Na-tional Level(Grassland Research Institute,Chinese Academy of Agricultur-al Sciences)National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China(2008BADB3B04)~~
文摘[Objective] The study aimed to understand the mutagenic effects of space flight on alfalfa seeds.[Method] Seeds of three lines of alfalfa were carried into orbit by the satellite 'Shijian-8' for space fight,the indices including seed germination rate,plant height and growth rate,were measured after the seeds been retrieved.[Result] Remarkable mutagenic effects occurred on the flight seeds,which were mainly presented by abnormal cotyledon,extended variation range of plant height and growth rate.Nineteen plants with increased plant height were preliminarily screened from the T0 progenies,but whether the mutated trait could inherit should be confirmed in further study.Three flight lines of alfalfa are different in the percentages of abnormal cotyledon(10%-18%),variation range of plant height(increased 30%-150% compared to control),selected plant number with increased plant height(5-7 plants),suggesting that differences exits between the three lines in mutation efficiency.[Conclusion] Valuable mutated materials could be obtained by space flight and applied in modern agriculture.
文摘The paper is given the interpolation of operators between weighted Hardy spaces and weighted L p spaces when w∈A 1 by Calderon Zygmund decomposition.
文摘In this paper we prove the following Hajek Renyi inequality:Let 0<p≤1 ,then for any Banach space B , any L p integrable B valued random variable sequence {D n,n≥1} ,any real number sequence {b n,n≥1} with 0<b n↑∞ ,any integer n≥1 ,there exits a constant C=C p>0 (only depending on p ) such thatP( sup j≥nji=1D ib j≥ε)≤Cε -p (∞j=n+1E‖D j‖ pb p j+nj=1E‖D j‖ pb p n) In the other direction,we prove some strong laws of large numbers and the integrability of the maximal functions for B valued random variable sequences by using this inequality and the Hajeck Renyi inequality we have obtained recently.Some known results are extended and improved.
基金supported by the National Natural Science Foundation of China(11571104)the Hunan Provincial Innovation Foundation for Postgraduate(CX2017B220)Supported by the Construct Program of the Key Discipline in Hunan Province
文摘In this article, the authors give a typical integral＇s bidirectional estimates for allcases. At the same time, several equivalent characterizations on the F（p, q, s, k） space in theunit ball of Cn are given.
基金Supported in part by the National Natural Science Foundation of China(11271359)the Fundamental Research Funds for the Central Universities(2014-Ia-037and 2015-IVA-069)
文摘This paper is devoted to characterizing the Riemann-Stieltjes operators and pointwise multipliers on F（p, q, s） spaces in the unit ball of C^n which contain many classical function spaces, such as the Bloch space, BMOA and Q8 spaces. The boundedness and compactness of these operators on F（p, q, s） spaces are characterized by means of an embedding theorem, i.e., F（p,q, s） spaces boundedly embedded into the tent-type spaces Tp,s^∞（μ）
基金supported by the National Natural Science Foundation of China(11571104)the Hunan Provincial Innovation Foundation for Postgraduate(CX2017B220)Supported by the Construct Program of the Key Discipline in Hunan Province
文摘LetΩ be a bounded symmetric domain in Cn. The purpose of this article is to define and characterize the general function space F（p, q, s） on Ω. Characterizing functions in the F（p, q, s） space is a work of considerable interest nowadays. In this article, the authors give several equivalent descriptions of the functions in the F（p, q, s） space on Ω in terms of fractional differential operators. At the same time, the authors give the relationship between F（p, q, s） space and Bloch type space on Ω too.
基金Supported by the National Natural Science Foundation of China (10771064)the Natural Science Foundation of Zhejiang province (Y6090036+1 种基金Y7080197,Y606197)the Foundation of Department of Education of Zhejiang Province (20070482)
文摘This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to Bers-type spaces Hv^∞（ or little Bers-type spaces Hv,o∞ ）, where v is normal.
基金Supported by the NNSF of China(10771064, 11101139)Supported by the NSF of Zhejiang Province(Y7080197, Y6090036, Y6100219)Supported by the Foundation of Creative Group in Colleges and Universities of Zhejiang Province(T200924)
文摘This paper deals with the boundedness and compactness of the compositionintegral type operators T g, from F (p, q, s) spaces to(little) Bloch-type spaces in the unit ball of C n , where Tg,φf(z) =∫01fφ(tz)Rg(tz)(dt)/t , z ∈ B, g ∈ H(B) and φ∈H(B, B).
