The aims of this study were to evaluate two methods, qPCR and a chemiluminescent assay (ColiLight II), for rapid detection of E. coli in water, and to examine the survival and persistence of clinical E. coli in drinki...The aims of this study were to evaluate two methods, qPCR and a chemiluminescent assay (ColiLight II), for rapid detection of E. coli in water, and to examine the survival and persistence of clinical E. coli in drinking water and biofilm using qPCR and ColiLight II. qPCR and ColiLight II were compared with a cultivation-based method (MPN), and survival and persistence of four clinical E. coli strains in water and biofilms on stainless steel (SS) and polyethylene (PE) surfaces were studied in a flow-through reactor with non-disinfected drinking water using ColiLight II, qPCR, ATP bioluminescence, and MPN. ColiLight II and qPCR correlated well with MPN. In drinking water, some clinical E. coli strains showed prolonged survival in drinking water flow-through systems, and persisted 3 - 3.4 times longer than the theoretical washout due to incorporation into biofilms. Strain specific attributes can significantly affect detection and persistence of E. coli in drinking water matrices.展开更多
Dyes are an integral part of the dying industry and have significantly resulted in environmental pollution by altering the standardwater quality after their discharge into the water bodies.The culprits behind the alte...Dyes are an integral part of the dying industry and have significantly resulted in environmental pollution by altering the standardwater quality after their discharge into the water bodies.The culprits behind the altered water quality are the pretreatment chemicals used during dying manufacturing processes.Various advanced treatmentmethods using conventional and advanced treatment options including solar energy have been put forth by researchers for the treatment of the dying effluents but,these methods have not proved significantly considerable.Therefore,the present study intends to check the efficiency of solar parabolic trough collector for treating the dying effluents in terms of color and COD.However,other wastewater parameters(BOD,nitrate and phosphate)have also been considered for this experimental analysis.Four standard flow rates(0.5,1.3,1.95 and 2.6 L/M)were maintained during the experiment for six hours at solar intensity(849.3±21.2W/m^(2))and the optimized flow rate(1.95 L/M)was detected and considered for further study during the present experiment.The color concentration showed a significant reduction(≥76.4%)by treatment with the designed solar reactor.Similar significant results were also noticed in terms of COD(79%).In this context,the current experimental study provides ideas on the decolorization and COD reduction efficiency with optimal flow rate in terms of cost-effectiveness with designed experimental reactor which could be further used and implemented for advanced scientific purposes.展开更多
文摘The aims of this study were to evaluate two methods, qPCR and a chemiluminescent assay (ColiLight II), for rapid detection of E. coli in water, and to examine the survival and persistence of clinical E. coli in drinking water and biofilm using qPCR and ColiLight II. qPCR and ColiLight II were compared with a cultivation-based method (MPN), and survival and persistence of four clinical E. coli strains in water and biofilms on stainless steel (SS) and polyethylene (PE) surfaces were studied in a flow-through reactor with non-disinfected drinking water using ColiLight II, qPCR, ATP bioluminescence, and MPN. ColiLight II and qPCR correlated well with MPN. In drinking water, some clinical E. coli strains showed prolonged survival in drinking water flow-through systems, and persisted 3 - 3.4 times longer than the theoretical washout due to incorporation into biofilms. Strain specific attributes can significantly affect detection and persistence of E. coli in drinking water matrices.
基金The authors would like to thank the National Fellowship for Scheduled Caste-University Grant Commission,Government of India for providing financial support.
文摘Dyes are an integral part of the dying industry and have significantly resulted in environmental pollution by altering the standardwater quality after their discharge into the water bodies.The culprits behind the altered water quality are the pretreatment chemicals used during dying manufacturing processes.Various advanced treatmentmethods using conventional and advanced treatment options including solar energy have been put forth by researchers for the treatment of the dying effluents but,these methods have not proved significantly considerable.Therefore,the present study intends to check the efficiency of solar parabolic trough collector for treating the dying effluents in terms of color and COD.However,other wastewater parameters(BOD,nitrate and phosphate)have also been considered for this experimental analysis.Four standard flow rates(0.5,1.3,1.95 and 2.6 L/M)were maintained during the experiment for six hours at solar intensity(849.3±21.2W/m^(2))and the optimized flow rate(1.95 L/M)was detected and considered for further study during the present experiment.The color concentration showed a significant reduction(≥76.4%)by treatment with the designed solar reactor.Similar significant results were also noticed in terms of COD(79%).In this context,the current experimental study provides ideas on the decolorization and COD reduction efficiency with optimal flow rate in terms of cost-effectiveness with designed experimental reactor which could be further used and implemented for advanced scientific purposes.
