Soluble Expression and Rapid Quantification of GFP-hepA Fusion Protein in Recombinant Escherichia coli

To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant （GFPmutl）. As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.

Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses： Potential and Challenges

Green fluorescent protein (GFP) and its variants /homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.

Predicting the subcellular location of apoptosis proteins based on recurrence quantification analysis and the Hilbert Huang transform

Apoptosis proteins play an important role in the development and homeostasis of an organism. The elucidation of the subcellular locations and functions of these proteins is helpful for understanding the mechanism of programmed cell death. In this paper, the recurrent quantification analysis, Hilbert-Huang transform methods, the maximum relevance and minimum redundancy method and support vector machine are used to predict the subcellular location of apoptosis proteins. The validation of the jackknife test suggests that the proposed method can improve the prediction accuracy of the subcellular location of apoptosis proteins and its application may be promising in other fields.

LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING(SPRI)

A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold surface.As a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low concentrations.We used SPRI as a label-free and parallel method to detect different proteins based on protein microarray.Bovine Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 matrix.These proteins can be discriminated directly by changing the incident angle of light.Excellent reproducibility for label-free detection of protein molecules was achieved.This SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry

Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities.However,this technique has several limitations and does,among others,not enable the precise identification of proteins.In this context,mass spectrometry(MS)became an alternative and orthogonal method that delivers qualitative and quantitative information on all identified HCPs.However,in order to be routinely implemented in biopharmaceutical companies,liquid chromatography-MS based methods still need to be standardized to provide highest sensitivity and robust and accurate quantification.Here,we present a promising MS-based analytical workflow coupling the use of an innovative quantification standard,the HCP Profiler solution,with a spectral library-based data-independent acquisition(DIA)method and strict data validation criteria.The performances of the HCP Profiler solution were compared to more conventional standard protein spikes and the DIA approach was benchmarked against a classical datadependent acquisition on a series of samples produced at various stages of the manufacturing process.While we also explored spectral library-free DIA interpretation,the spectral library-based approach still showed highest accuracy and reproducibility(coefficients of variation<10%)with a sensitivity down to the sub-ng/mg mAb level.Thus,this workflow is today mature to be used as a robust and straightforward method to support mAb manufacturing process developments and drug products quality control.

Label-Free and Real-Time Monitor of Binding and Dissociation Processes between Protein A and Swine IgG by Oblique-Incidence Reflectivity Difference Method

Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference （OIRD） in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.

Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum.To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum,label-free quantification analysis was used to analyze the proteome changes after apical bud removal.Quantitative real-time PCR(qPCR)was used to analyze the changes in the expression of three plant hormone-related genes.A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation.The number of differentially expressed proteins in the three stages(24 h/0 h,48 h/0 h,48 h/24 h)were 219,332,and 97,respectively.The difference in expressed proteins in the three comparison stages mainly involves RNA processing and modification;translation,ribosomal structure and biogenesis;Posttranslational modification,protein turnover,and chaperones.Path analysis showed that there was various physiological activities in the process of lateral bud dormancy breaking and elongation,which involved energy metabolism,biosynthesis,signal transduction and stress response in the growth process of lateral buds.qPCR indicated that the expression of cytokinin synthesis related gene was significantly increased after the removal of apical dominance,while the expression of strigolactones synthesis related gene experiences a dramatic fall to promote the development of the lateral buds.However,there was a drop before a slight increase in the expression of the auxin synthesis related gene,which was mainly due to the removal of apical dominance that led to the loss of indoleacetic acid in the main stem.However,with formation of the new apical source,indoleacetic acid can be released again.

Observation on Culture Characteristics of Mycoplasma bovis with Four Different Antigen Quantification Methods

[Objective] The paper was to explore the antigen harvest time of Mycoplasrna bovis and the antigen quantification alternative method. [Method] M. bovis 08M strain was inoculated in the Thiaueourt＇s medium containing 10% horse serum. Four growth curves were plotted by simultaneously measuring color change units （CCU）, colony forming units （CFU）, protein concentration and nucleic acid levels within 110 h. [ Result] The growth of M. bovis was divided into four phases： the longarithmie phase appeared after being cultured for 10 h; the stationary phase appeared at 30 h with the highest number of viable cells up to 1. 0× 108 CCU/mL and 7.7 × 107 CCU/mL, respectively; and the decline phase started at 75 h. The protein concentration afM. bov/s increased rapidly from 15 to 35 h, reached 72.06 μg/mL at 35 h, then maintained at 53.38 - 70.65 μg/mL. The nucleic acid levels of M. bov/s increased rapidly from 15 h, and the cycle threshold （Ct） values were maintained between 15, 32 and 17.84 after 25 h. [ Conclusion] There was a good correlation between the protein concentration and variable count of M. bov/s at the early stationary phase, which was the best time period to harvest antigen. The protein concentration determination could be an alternative method to quantify antigen content of M. bovis when preparing inactivated M. boviz vaccine. Key words Mycoplasma boyis; Antigen quantification; Color change units; Colony forming units; Protein concentration; Nucleic acid content

Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method

Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods.Herein,we used a new strategy:data-dependent acquisition mode identification combined with label-free quantification by SWATH acquisition mode,to study the differentially expressed proteins in mouse liver cancer metastasis cells.A total of 1528 protein groups were identified,among which 1159 protein groups were quantified and 249 protein groups were observed as differentially expressed proteins(86 proteins up-regulated and 163 down-regulated).This method provides a commendable solution for the identification and quantification of differentially expressed proteins in biological samples.

A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum

Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.

Endonuclease-based Method for Detecting the Sequence Specific DNA Binding Protein on Double-stranded DNA Microarray

The double-stranded DNA (dsDNA) probe contains two different protein binding sites. One is for DNA- binding proteins to be detected and the other is for a DNA restriction enzyme. The two sites were arranged together with no base interval. The working principle of the capturing dsDNA probe is described as follows: the capturing probe can be cut with the DNA restriction enzyme (such as EcoR I) to cause a sticky terminal, if the probe is not bound with a target protein, and the sticky terminal can be extended and labeled with Cy3-dUTP by DNA polymerase. When the probe is bound with a target protein, the probe is not capable to be cut by the restriction enzyme because of space obstruction. The amount of the target DNA binding proteins can be measured according to the variations of fluorescent signals of the corresponding probes.

超声声脉冲辐射力成像-声触诊组织定量技术、丝氨酸/苏氨酸蛋白激酶基因检测与甲状腺弥漫性病变合并甲状腺癌中医证型的关系及诊断效能

目的分析超声声脉冲辐射力成像-声触诊组织定量技术(ARFI-VTQ)、B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)基因检测对甲状腺弥漫性病变合并甲状腺癌的诊断效能,以及与甲状腺癌中医证型的关系。方法选择2023年1月至2023年12月于河北省沧州中西医结合医院超声医学一科进行甲状腺弥漫性病变合并甲状腺结节检查的患者130例作为研究对象,根据病理检查结果分为良性组(n=88)和恶性组(n=42),所有患者均行超声ARFI-VTQ检查和BRAF基因检测,并进行中医辨证分型。比较良性组与恶性组、恶性组不同中医证型患者常规超声特征、SWV值以及BRAF基因检测阳性率;行受试者工作特征(ROC)曲线分析,分析超声ARFI-VTQ和BRAF基因检测对甲状腺弥漫性病变合并甲状腺癌的诊断效能。结果良性组与恶性组患者,以及恶性组气滞血瘀证、肝郁痰凝证、气阴两虚证患者常规超声特征的边界、形状、回声和内部血流比较差异均无统计学意义(P>0.05)。恶性组SWV值高于良性组(P<0.05);恶性组不同中医证型患者SWV值也存在明显差异,其中气滞血瘀证最高,气阴两虚证最低,比较差异有统计学意义(P<0.05)。良性组BRAF基因突变阳性率低于恶性组(P<0.05);恶性组不同中医证型患者BRAF基因突变阳性率比较差异无统计学意义(P>0.05)。ROC曲线分析结果显示,SWV值和BRAF基因检测结果对甲状腺弥漫性病变合并甲状腺癌的曲线下面积(AUC)分别为0.759和0.674,敏感度分别为88.10%和76.19%,特异性分别为90.17%和84.97%,约登指数分别为0.783和0.612。但是联合检测降低了检测的特异性,导致约登指数降低为0.369。结论超声ARFI-VTQ检查、BRAF基因检测对甲状腺弥漫性病变合并甲状腺癌的诊断具有重要的价值,其中ARFI-VTQ检查对甲状腺弥漫性病变合并甲状腺癌患者的辨证分型有重要的参考价值。

Circulating proteomic biomarkers for diagnosing sporadic amyotrophic lateral sclerosis:a cross-sectional study

Biomarke rs are required for the early detection,prognosis prediction,and monitoring of amyotrophic lateral sclerosis,a progressive disease.Proteomics is an unbiased and quantitative method that can be used to detect neurochemical signatures to aid in the identification of candidate biomarke rs.In this study,we used a label-free quantitative proteomics approach to screen for substantially differentially regulated proteins in ten patients with sporadic amyotrophic lateral scle rosis compared with five healthy controls.Su bstantial upregulation of serum proteins related to multiple functional clusters was observed in patients with spo radic amyotrophic lateral sclerosis.Potential biomarke rs were selected based on functionality and expression specificity.To validate the proteomics profiles,blood samples from an additional cohort comprising 100 patients with sporadic amyotrophic lateral sclerosis and 100 healthy controls were subjected to enzyme-linked immunosorbent assay.Eight substantially upregulated serum proteins in patients with spora dic amyotrophic lateral sclerosis were selected,of which the cathelicidin-related antimicrobial peptide demonstrated the best discriminative ability between patients with sporadic amyotrophic lateral sclerosis and healthy controls(area under the curve[AUC]=0.713,P<0.0001).To further enhance diagnostic accuracy,a multi-protein combined discriminant algorithm was developed incorporating five proteins(hemoglobin beta,cathelicidin-related antimicrobial peptide,talin-1,zyxin,and translationally-controlled tumor protein).The algo rithm achieved an AUC of 0.811 and a P-value of<0.0001,resulting in 79%sensitivity and 71%specificity for the diagnosis of sporadic amyotrophic lateral scle rosis.Subsequently,the ability of candidate biomarkers to discriminate between early-stage amyotrophic lateral sclerosis patients and controls,as well as patients with different disease severities,was examined.A two-protein panel comprising talin-1 and translationally-controlled tumor protein effectively distinguished early-stage amyotrophic lateral sclerosis patients from controls(AUC=0.766,P<0.0001).Moreove r,the expression of three proteins(FK506 binding protein 1A,cathelicidin-related antimicrobial peptide,and hemoglobin beta-1)was found to increase with disease progression.The proteomic signatures developed in this study may help facilitate early diagnosis and monitor the progression of sporadic amyotrophic lateral sclerosis when used in co mbination with curre nt clinical-based parameters.

Gehua Jiejiu Dizhi decoction(葛花解酒涤脂汤)ameliorates alcoholic fatty liver in mice by regulating lipid and bile acid metabolism and with exertion of antioxidant stress based on 4DLabel-free quantitative proteomic study

OBJECTIVE:To analyze the effect and molecular mechanism of Gehua Jiejiu Dizhi decoction(葛花解酒涤脂汤,GJDD)on alcoholic fatty live disease(AFLD)by using proteomic methods.METHODS:The male C57BL/6J mouse were randomly divided into four groups:control group,model group,GJDD group and resveratrol group.After the AFLD model was successfully prepared by intragastric administration of alcohol once on the basis of the Lieber-DeCarli classical method,the GJDD group and resveratrol group were intragastrically administered with GJDD(4900 mg/kg)and resveratrol(400 mg/kg)respectively,once a day for 9 d.The fat deposition of liver tissue was observed and evaluated by oil red O(ORO)staining.4DLabel-free quantitative proteome method was used to determine and quantify the protein expression in liver tissue of each experimental group.The differentially expressed proteins were screened according to protein expression differential multiples,and then analyzed by Gene ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway enrichment.Finally,expression validation of the differentially co-expressed proteins from control group,model group and GJDD group were verified by targeted proteomics quantification techniques.RESULTS:In semiquantitative analyses of ORO,all kinds of steatosis(ToS,MaS,and MiS)were evaluated higher in AFLD mice compared to those in GJDD or resveratroltreated mice.4DLabel-free proteomics analysis results showed that a total of 4513 proteins were identified,of which 3763 proteins were quantified and 946 differentially expressed proteins were screened.Compared with the control group,145 proteins were up-regulated and 148 proteins were down-regulated in the liver tissue of model group.In addition,compared with the model group,92 proteins were up-regulated and 135 proteins were downregulated in the liver tissue of the GJDD group.15 differentially co-expressed proteins were found between every two groups(model group vs control group,GJDD group vs model group and GJDD group vs control group),which were involved in many biological processes.Among them,11 differentially co-expressed key proteins(Aox3,H1-5,Fabp5,Ces3a,Nudt7,Serpinb1a,Fkbp11,Rpl22l1,Keg1,Acss2 and Slco1a1)were further identified by targeted proteomic quantitative technology and their expression patterns were consistent with the results of 4D label-free proteomic analysis.CONCLUSIONS:Our study provided proteomics-based evidence that GJDD alleviated AFLD by modulating liver protein expression,likely through the modulation of lipid metabolism,bile acid metabolism and with exertion of antioxidant stress.

Spike蛋白介导细胞融合程度定量方法的建立及其在药物筛选中的应用

新型冠状病毒Spike蛋白(S蛋白)与受体ACE2结合介导细胞融合的发生,为进一步探究新冠感染合胞体发生、发展的规律及致病机制,明确关键蛋白靶点,筛选干预合胞体形成的药物,笔者拟构建新冠S蛋白介导的细胞融合程度的定量方法.利用pCDH-CMV-MCS-EF1-Puro、pLV-CMV-MCS-3FLAG-IRES-Bla、S蛋白基因序列、ACE2基因序列、LacZ基因序列、EcoRⅠ限制性内切酶及同源重组酶构建慢病毒穿梭质粒pCDH-S,pCDH-ACE2,pLV-α与pLV-ω,利用该慢病毒质粒分别构建稳定表达S蛋白与ACE2蛋白的293FT和A549细胞系,随后利用pLV-α与pLV-ω慢病毒质粒分别感染上述稳定细胞系,构建出可以定量细胞融合程度的细胞系293FT-S_(1-56),A549-S_(1-56),293FT-ACE2△_(11-41),A549-ACE2△_(11-41).最后采用Western Blot蛋白免疫印迹技术鉴定上述融合定量细胞系中S蛋白与ACE2蛋白的过表达效果,并通过细胞共培养确定融合效果,用β-半乳糖苷酶报告基因检测试剂盒定量细胞融合程度.结果显示,慢病毒载体和稳定细胞系构建成功,时间梯度、转染梯度实验显示该系统具有良好的定量灵敏度,并能用于卡莫司他(Camostat mesylate)和氯硝柳胺(Niclosamide)等药物抗融合效果的定量评价.成功构建的新冠病毒Spike蛋白介导细胞融合程度定量系统,可用于不同S蛋白突变体致融合能力评价和抗融合药物筛选评价,可为进一步的研究奠定技术基础.

mAlb联合CysC、HbA1c和uNGAL在2型糖尿病早期肾损伤中的诊断价值

目的探讨尿微量白蛋白(mAlb)联合血清胱抑素C(Cys C)、糖化血红蛋白(HbA1c)和尿液中性粒细胞明胶酶相关脂质运载蛋白(uNGAL)在2型糖尿病(T2DM)早期肾损伤中的诊断价值。方法选择2020年8月至2022年8月就诊于镇平县中医院的105例2型糖尿病患者作为研究对象,根据患者是否合并早期肾损伤将其分为肾损伤组和非肾损伤组。对比两组患者尿mAlb、血清Cys C、HbA1c和uNGAL水平。采用Kendall’s tau-b(K)检验分析mAlb、Cys C、HbA1c、uNGAL与T2DM肾损伤的相关性;绘制ROC曲线,分析并比较尿mAlb、血清Cys C、HbA1c、uNGAL单独及联合预测2型糖尿病早期肾损伤的价值。结果肾损伤组的mAlb、Cys C、HbA1c和uNGAL水平均比非肾损伤组高,差异具有统计学意义(P<0.05)。Kendall’s tau-b(K)检验发现,mAlb、Cys C、HbA1c、uNGAL水平与T2DM肾损伤的发生呈正相关,差异具有统计学意义(r=0.669、0.534、0.478、0.569,P<0.05)。Cys C、HbA1c和uNGAL单独及联合预测T2DM早期肾损伤发生的AUC值分别为0.737(95%CI:0.639~0.834)、0.769(95%CI:0.677~0.861)、0.691(95%CI:0.589~0.793)、0.730(95%CI:0.630~0.831)、0.816(95%CI:0.736~0.896);特异度分别为0.793、0.810、0.724、0.759、0.948,灵敏度分别为0.617、0.632、0.598、0.621、0.620;联合预测T2DM早期肾损伤发生的AUC分别高于Cys C、HbA1c和uNGAL单独检测(Z=8.632、3.152、5.324、5.386,P=0.000、0.012、0.000、0.000、0.000)。结论尿mAlb、血清Cys C、HbA1c和uNGAL在T2DM早期肾损伤患者中表达升高,上述指标与T2DM早期肾损伤的发生呈正相关,通过联合应用上述指标可提高疾病的诊断效果。

IgA肾病患者随机尿中ACR与24hUTP定量检测的相关性及其对临床诊断的一致性分析

目的探讨IgA肾病(immunoglobulin A nephropathy,IgAN)患者随机尿清蛋白/肌酐比值(albumin-to-creatinine ratio,ACR)与24 h尿蛋白定量(24 hour urine total protein quantification,24hUTP)检测之间的相关性及其对临床诊断的一致性分析。方法选取北京大学深圳医院2019年1月~2020年12月收治的230例原发性IgAN患者作为研究对象。采用相关性分析和组内相关系数(intraclass correlation coefficient,ICC)分析ACR与24hUTP间的相关性及其对临床诊断的一致性。应用不同慢性肾脏病(chronic kidney disease,CKD)分期和尿蛋白水平进行亚组分析。以24hUTP=0.5 g(24h),1.0 g(24h)和3.5 g(24h)为界点绘制受试者工作特征(receiver operating characteristic,ROC)曲线,确定ACR的cut-off值。结果IgAN患者ACR[0.79(0.41~1.45)g/g]与24 hUTP[1.02(0.58~1.80)g/24h]呈正相关(r=0.85,P<0.01),二者在临床诊断上的一致性程度中等(ICC=0.63,P<0.01)。亚组分析结果显示,二者的相关性和一致性不受CKD分期影响,不同CKD分期的二者相关系数(r)在0.76~0.86之间(均P<0.01),组内相关系数(interclass correlation coefficient,ICC)在0.53~0.72之间;受尿蛋白水平的影响,当24 hUTP≤0.5 g/24h时,二者无相关性(r=0.08,P>0.05),且在24 hUTP≤0.5 g/24h,0.5 g/24h<24hUTP≤1 g/24h和24 hUTP>3.5 g/24h三组中,二者的一致性可忽略(ICC均<0.20)。ROC曲线分析结果显示,当24 hUTP=0.5 g/24h,1.0 g/24h和3.5 g/24h时,ACR分别为0.30 g/g,0.57 g/g和1.28 g/g时为其cut-off值。结论IgAN患者中,ACR不能简单地替代24 hUTP进行尿蛋白水平评估。特别是在24hUTP≤1 g/24h和24 hUTP>3.5 g/24h的时候,ACR不能准确地反映真实的尿蛋白水平。

肾复康Ⅱ号联合氯沙坦钾对IgAN伴肾小管间质纤维化患者尿蛋白定量与中医证候积分的影响

目的:探讨肾复康Ⅱ号胶囊联合氯沙坦钾片对免疫球蛋白A肾病(immunoglobulin A nephropathy,IgAN)伴肾小管间质纤维化患者尿蛋白定量与中医证候积分的影响及其相关性。方法:将80例IgAN伴肾小管间质纤维化患者分为对照组和试验组各40例,对照组予氯沙坦钾片治疗,试验组在对照组基础上予肾复康Ⅱ号胶囊,观察两组患者治疗12、24周后尿蛋白定量及治疗前后中医证候积分。结果:与治疗前比较,试验组治疗12、24周后尿蛋白定量下降(P<0.05),对照组治疗24周尿蛋白定量下降(P<0.05);与对照组比较,试验组治疗12、24周尿蛋白定量降低(P<0.05);治疗24周后试验组中医证候积分较治疗前降低(P<0.05)。结论:IgAN伴肾小管间质纤维化患者尿蛋白定量与中医证候积分之间存在相关性,尿蛋白定量及预后与中医证候、总有效率具有一致性。

拉贝洛尔在妊娠期高血压患者中的应用效果

目的:探究拉贝洛尔在妊娠期高血压患者中的应用效果。方法:选取2022年1月—2023年12月黔南布依族苗族自治州惠水县中医医院收治的妊娠期高血压患者68例为研究对象,采用随机抽签方式分为对照组和观察组,各34例。对照组采用常规治疗,观察组在对照组基础上联合拉贝洛尔治疗。比较两组收缩压、舒张压、24 h尿蛋白定量水平,治疗效果,不良反应发生率。结果:治疗前,两组收缩压、舒张压、24 h尿蛋白定量水平比较,差异无统计学意义(P>0.05);治疗后,两组收缩压、舒张压、24 h尿蛋白定量水平降低,且观察组低于对照组,差异有统计学意义(P<0.05)。观察组治疗优良率高于对照组,差异有统计学意义(P=0.033)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:在常规治疗基础上采用拉贝洛尔治疗妊娠期高血压,降压效果、安全性较好,可降低24 h尿蛋白定量水平。

黄葵胶囊联合氢氯噻嗪片治疗慢性肾小球肾炎的效果及对患者24 h尿蛋白定量、氧化应激的影响

目的探究氢氯噻嗪片、黄葵胶囊联合治疗慢性肾小球肾炎的效果及对患者24 h尿蛋白定量、氧化应激的影响。方法选取兰陵县人民医院2021年4月—2023年6月收治的84例慢性肾小球肾炎患者为研究对象,使用随机数字表法将其分为对照组与观察组,各42例。对照组接受氢氯噻嗪片治疗,观察组接受黄葵胶囊、氢氯噻嗪片联合治疗,两组治疗时间均为2个月。比较两组的临床疗效、肾功能指标、24 h尿蛋白定量、氧化应激指标及不良反应发生情况。结果观察组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。治疗前,两组肾功能及氧化应激各项指标水平比较,组间差异无统计学意义(P>0.05);治疗后,观察组血肌酐、尿素氮及血清丙二醛水平均低于对照组,血清超氧化物歧化酶水平高于对照组,组间差异有统计学意义(P<0.05);治疗前,两组患者24 h尿蛋白定量比较,差异无统计学意义(P>0.05);治疗1、2个月后,观察组24 h尿蛋白定量水平均低于对照组,组间差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论对慢性肾小球肾炎联用黄葵胶囊、氢氯噻嗪片治疗的效果显著,可促使患者的肾功能改善,调节24 h尿蛋白定量,减轻氧化应激反应,且安全性良好。