Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum.To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum,label-free quantification analysis was used to analyze the proteome changes after apical bud removal.Quantitative real-time PCR(qPCR)was used to analyze the changes in the expression of three plant hormone-related genes.A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation.The number of differentially expressed proteins in the three stages(24 h/0 h,48 h/0 h,48 h/24 h)were 219,332,and 97,respectively.The difference in expressed proteins in the three comparison stages mainly involves RNA processing and modification;translation,ribosomal structure and biogenesis;Posttranslational modification,protein turnover,and chaperones.Path analysis showed that there was various physiological activities in the process of lateral bud dormancy breaking and elongation,which involved energy metabolism,biosynthesis,signal transduction and stress response in the growth process of lateral buds.qPCR indicated that the expression of cytokinin synthesis related gene was significantly increased after the removal of apical dominance,while the expression of strigolactones synthesis related gene experiences a dramatic fall to promote the development of the lateral buds.However,there was a drop before a slight increase in the expression of the auxin synthesis related gene,which was mainly due to the removal of apical dominance that led to the loss of indoleacetic acid in the main stem.However,with formation of the new apical source,indoleacetic acid can be released again.

The application of label-free imaging technologies in transdermal research for deeper mechanism revealing

The penetration behavior of topical substances in the skin not only relates to the transdermal delivery efficiency but also involves the safety and therapeutic effect of topical products,such as sunscreen and hair growth products.Researchers have tried to illustrate the transdermal process with diversified theories and technologies.Directly observing the distribution of topical substances on skin by characteristic imaging is the most convincing approach.Unfortunately,fluorescence labeling imaging,which is commonly used in biochemical research,is limited for transdermal research for most topical substances with a molecular mass less than 500 Da.Label-free imaging technologies possess the advantages of not requiring any macromolecular dyes,no tissue destruction and an extensive substance detection capability,which has enabled rapid development of such technologies in recent years and their introduction to biological tissue analysis,such as skin samples.Through the specific identification of topical substances and endogenous tissue components,label-free imaging technologies can provide abundant tissue distribution information,enrich theoretical and practical guidance for transdermal drug delivery systems.In this review,we expound the mechanisms and applications of the most popular label-free imaging technologies in transdermal research at present,compare their advantages and disadvantages,and forecast development prospects.

The Marking Technology in Motion Capture for the Complex Locomotor Behavior of Flexible Small Animals(Gekko gecko)

Animals have evolved a variety of behavior patterns to adapt to the environment. Motion-capture technology is utilized to quantify and characterize locomotor behaviors to reveal the mechanisms of animal motion. In the capture of flexible, small animals with complex locomotor behaviors, the markers interfere with each other easily, and the motion forms(bending, twisting) of the moving parts are obviously different;thus, it is a great challenge to realize accurate quantitative characterization of complex locomotor behaviors. The correlation between the marker properties, including the size and space length, and the precision of the system are revealed in this paper, and the effects of diverse marker shapes on the capturing accuracy of the captured objects in different motion forms were tested. Results showed that the precision of system is significantly improved when the ratio of the space length to the diameter of the markers is larger than four;for the capture of the spatial twisting motion of the flexible object, the hexagon markers had the lowest spatial lost-marker rate relative to the circle, triangle, and square. Customized markers were used to capture the locomotor behavior of the gecko-inspired robot(rigid connection) and the gecko(flexible connection). The results showed that this marking technology can achieve high accuracy of motion capture for geckos(the average deviation was approximately 0.32 mm, and the average deviation’s variation rate was approximately 0.96%). In this paper, the marking technology for the motion capture of flexible, small animals with complex motion is proposed;it can effectively improve the system precision as well as the capture accuracy, and realize the quantitative characterization of the complex motion of flexible, small objects. It provides a reliable technical means to deeply study the evolution of the motion function of small animals and advance systematic research of motion-capture technology.

Evaluation of effect of acupuncture treatment on carotid arteriosclerosis plaque by three-dimensional ultrasound VPQ technology

Objective:To evaluate the structural changes of atherosclerotic plaque before and after acupuncture treatment by three-dimensional ultrasound of Vascular Plaque Quantification technology.Methods:Forty subjects were recruited and randomly divided into acupuncture group and sham acupuncture group.All patients were treated twice a week for 3 months and were observed the changes of the bilateral carotid plaque volume(PV),gray scale value(GSM)and inner media thickness(IMT).Results:The acupuncture group’s PV decreased after 3 months,while the sham group’s increased slightly.The result were statistically significant(P≤0.05).After treatment,the acupuncture group’s left GSM was higher than before,while the sham group’s was slightly lower than before.The result were statistically significant(P≤0.05).Although the difference of the acupuncture group’s right GSM before and after treatment was not statistically significant,it also had a rising trend.Conclusion:Acupuncture can reduce the volume of carotid atherosclerotic plaque and stabilize the nature of the plaque.

四维左房定量分析技术评价隐匿性未控制高血压患者左房结构和功能的临床价值

目的探讨四维左房定量分析技术(4D-Auto-LAQ)评价隐匿性未控制高血压(MUHT)患者左房结构和功能的临床应用价值。方法选取于我院行24 h动态血压监测的隐匿性高血压患者71例,其中MUHT者34例(MUHT组),血压控制良好者37例(WCHT组)。应用常规超声心动图获取左房内径(LAD)、室间隔厚度(IVST)、左室后壁厚度(LVPWT)、左室舒张末容积(LVEDV)、左室射血分数(LVEF)、二尖瓣口舒张早期和晚期血流速度(E和A)、左室侧壁运动速度(Lat e’)及室间隔运动速度(Sep e’),计算Lat e’和Sep e’的平均值(e’)和E/e’;4D-Auto-LAQ获取左房容积参数和左房应变参数,其中左房容积参数包括左房最大容积指数(LAVImax)、左房收缩前容积指数(LAVIpreA)、左房最小容积指数(LAVImin)、左房总射血分数(LAEF)、左房被动射血分数(LApEF)、左房主动射血分数(LAaEF),左房应变参数包括左房储备期应变(LASr)、左房管道期应变(LAScd)、左房收缩期应变(LASct);比较两组上述各参数的差异;分析LASr、LAScd、LASct与E/e’的相关性。结果与WCHT组比较,MUHT组Lat e’、Sep e’、e’均减低,E/e’增加,差异均有统计学意义(均P<0.05);两组LAD、IVST、LVPWT、LVEDV、LVEF、E、A比较差异均无统计学意义。与WCHT组比较,MUHT组LAVImax、LAVIpreA、LAVImin、LAaEF均增加,LApEF、LASr、LAScd、LASct均减低,差异均有统计学意义(均P<0.05)。相关性分析显示,LASr与E/e’呈正相关(r=0.393,P<0.001),LAScd、LASct与E/e’均无明显相关性。结论4D-Auto-LAQ可早期发现MUHT患者左房结构和功能变化,具有一定的临床应用价值。

基层中医药适宜技术遴选指标体系的构建

目的:构建基层中医药适宜技术评价遴选指标体系。方法:对山东、四川等11个代表性省份进行调研和文献研究,收集、汇总已有的基层中医药适宜技术相关遴选指标体系;组织权威专家对既往基层中医药适宜技术相关遴选指标体系遴选维度和遴选指标进行合并、增删等操作,初步得出基层中医药适宜技术相关遴选指标体系;组织专家开展初步遴选,收集专家对遴选指标体系的意见;组织15个省(区、市)开展正式遴选,收集各省份对遴选指标体系的建议。结果:从安全性、有效性、规范性、经济性、推广性5个维度构建了基层中医药适宜技术遴选指标体系,该遴选指标体系包括5个一级指标,12个二级指标和54个三级指标,且实现了量化评分。结论:本研究建立的基层中医药适宜技术遴选指标体系具有科学、系统和可操作性强等特点,可为基层中医药适宜技术的遴选提供参考工具。

全方位夯实粮食安全根基——主要指标及关联程度测度分析

文章理论阐释全方位夯实粮食安全根基的政策涵义,构建四维度34个指标的粮食安全根基综合评价指标体系。基于2011—2021年时间序列数据,文章采用牛顿第二定律、灰色关联度等模型,揭示各指标对粮食安全根基的驱动力强弱,测度各指标与粮食安全根基的关联程度及关键指标的变化趋势。研究发现,在夯实粮食安全根基的驱动力作用下,耕地保护和科技支撑起到正向作用,党政同责夯实的作用呈波动趋势,而种粮收益则表现为负向作用。应稳定党政同责保障粮食安全的作用,逐步将支持的重点转向农业科技进步,通过科技支撑带动耕地保护程度和种粮收益水平持续提高。我国粮食安全根基综合指数呈“W”形变动,但科技支撑指数呈现的复杂趋势和种粮收益指数相对稳定在较低水平的态势要引起重视。未来耕地质量和科技支撑对粮食安全的作用将不断增强,要重点防范耕地数量逐渐减少和“谁来种粮”问题带来的粮食安全风险。

基于人工智能的自动应变定量技术评价慢性肾病患者早期心肌损伤的临床价值

目的探讨基于人工智能的自动应变定量技术评价慢性肾病(CKD)患者早期心肌损伤的临床价值。方法选取2021年7月至2023年7月我院收治的CKD患者125例为研究对象,根据CKD分期分别纳入A组(2~3期,67例)、B组(4~5期,58例),另选取同期45例健康体检者为对照组。比较3组二维超声心动图参数[左室射血分数(LVEF)、舒张末期室间隔厚度(IVSd)、舒张末期左室后壁厚度(LVPWd)、左心室舒张末期内径(LVEDD)、右心室内径(RVD)]、血清N末端脑钠肽前体(NT-pro BNP)水平、自动及手动应变定量技术应变参数[左心室整体纵向收缩期峰值应变(LVGLS)、右心室整体纵向收缩期峰值应变(RVGLS)、左房储备期纵向应变(LASr)、左房管道期纵向应变(LAScd)、左房收缩期纵向应变(LASct)],采用Pearson分析自动应变参数与手动应变参数、二维超声心动图参数及血清NT-pro BNP水平的相关性,比较LVGLS、RVGLS、左房应变分析的手动、自动应变分析时间,采用受试者工作特征(ROC)曲线分析自动应变参数联合检测对CKD患者早期心肌损伤的诊断价值。结果B组LVEF水平低于A组、对照组,B组IVSd、LVPWd、LVEDD、RVD及血清NT-pro BNP水平高于A组、对照组,且A组高于对照组,差异有统计学意义(P<0.05);B组自动及手动LVGLS、RVGLS、LAScd、LASct均高于A组、对照组,B组LASr低于A组、对照组,且A组自动LVGLS、RVGLS、LAScd、LASct高于对照组,LASr低于对照组,A组手动LAScd、LASct高于对照组,LASr低于对照组,差异有统计学意义(P<0.05);自动分析各应变参数与手动分析各参数均呈正相关(r=0.852,0.721,0.605,0.630,0.627,P<0.05);自动LVGLS、RVGLS、LAScd、LASct与LVEF呈负相关(r=-0.659,-0.598,-0.612,-0.601,P<0.05),与NT-pro BNP呈正相关(r=0.643,0.638,0.622,0.647,P<0.05);自动LASr与LVEF呈正相关(r=0.629,P<0.05),与NT-pro BNP呈负相关(r=-0.658,P<0.05)。自动病变分析时间较手动分析时间明显减少,差异有统计学意义(P<0.05)。LVGLS诊断CKD患者早期心肌损伤的曲线下面积(AUC)为0.928(95%CI:0.889~0.957),约登指数为0.736,最佳诊断敏感度为87.20%,特异度为86.40%,差异有统计学意义(P<0.05)。结论自动应变定量技术应变参数联合检测对CKD患者早期心肌损伤具有一定诊断价值,可作为临床评估早期心肌损伤的有效检查方式,并对后续治疗具有一定指导价值。

我国科技成果转化政策演变特征及效用研究

科技成果转化政策是引导和规范科技成果转化活动的制度基础,全面把握科技成果转化政策的演变特征及效用,可以为政策内容优化与效用提升提供决策支持。通过一套基于政策文本内容挖掘的科技成果转化政策演变特征及效用分析框架,对2000—2021年我国科技成果转化政策文本数据进行分析。分析结果显示,我国科技成果转化政策中呈现强化趋势的有“运行”“资金”“机制”等,呈现弱化趋势的有“机构”“项目”等,呈现新兴趋势的有“人员”“平台”等;“基金管理”“院校成果转化”“奖励机制”等主题发展较为成熟且热度持续上升,“政策落实”“人员管理”“孵化器”等主题具有较大发展潜力和空间;政策密度、政策力度、政策强度均呈现增长趋势,且均对技术成果市场化具有显著的促进作用。

中国科技服务业政策文本量化评价——基于PMC指数模型的分析

科技服务业的高质量发展需要多方位的政策支持。对科技服务业政策进行量化评价可以发现政策短板,有助于政策的优化和调整。以2008—2021年中国117项科技服务业政策为研究对象,运用ROST CM6软件,采用文本挖掘方法提取政策文本的高频词,构建语义关系网,归纳出八大政策类属,构建科技服务业政策的评价指标体系,运用PMC指数模型对省级政策进行评价分析,提出优化路径。研究发现:24份政策文件总体处于可接受水平,4份优秀,16份良好,均未达到完美级别。研究发现了政策文本的共性问题:政策目标、开放合作和人才支持为政策短板;政策文本的评级越高,其政策内容的一致性越高;同一地区不同时期的政策保持连贯性有利于提升其政策等级。研究结论可为政府制定与完善科技服务业政策提供科学、明确的优化路径。

四维自动左房定量技术评价不同透析方式对尿毒症患者左房结构和功能的影响

目的应用四维自动左房定量技术(4D Auto LAQ)评价不同透析方式对尿毒症患者左房结构和功能的影响。方法选取于我院肾内科就诊的尿毒症患者80例,根据透析方式分为血液透析组39例和腹膜透析组41例,另选同期健康体检者35例作为正常对照组。应用常规超声心动图获取左室射血分数(LVEF)、左房内径(LAD)、左室舒张末期内径(LVEDD)、室间隔厚度(IVS)、左室后壁厚度(LVPW);4D Auto LAQ获取左房应变参数,包括左房储备期纵向应变(LASr)、左房管道期纵向应变(LAScd)、左房收缩期纵向应变(LASct)、左房储备期环形应变(LASr-c)、左房管道期环形应变(LAScd-c)、左房收缩期环形应变(LASct-c),以及左房容积参数,包括左房最大容积(LAVmax)、左房最小容积(LAVmin)、左房收缩前容积(LAVpreA)、左房射血分数(LAEF),比较各组上述参数的差异;分析LAEF与左房应变参数的相关性。结果①各组常规超声心动图参数比较:腹膜透析组和血液透析组LAD、LVEDD、IVS、LVPW均较正常对照组增大,差异均有统计学意义(均P<0.05);各组LVEF比较差异无统计学意义。②各组4D Auto LAQ左房应变参数比较:与正常对照组比较,腹膜透析组LASr、LAScd、LASr-c、LAScd-c均减小,LASct、LASct-c均增大,血液透析组LASr、LAScd、LASct、LASr-c、LAScd-c、LASct-c均减小,差异均有统计学意义(均P<0.05);除LAScd外,血液透析组LASr、LAScd、LASct、LASr-c、LAScd-c、LASct-c均较腹膜透析组减小,差异均有统计学意义(均P<0.05)。③各组4D Auto LAQ左房容积参数比较:与正常对照组比较,腹膜透析组LAVmax、LAVmin、LAVpreA均增大,LAEF减小,血液透析组LAVmax、LAVmin、LAVpreA均增大,LAEF减小,差异均有统计学意义(均P<0.05);与腹膜透析组比较,血液透析组LAVmax、LAmin、LAVpreA均增大,LAEF减小,差异均有统计学意义(均P<0.05)。④相关性分析显示,LAEF与LASr、LAScd、LASr-c、LAScd-c、LASct、LASct-c均呈正相关(r=0.531、0.522、0.705、0.686、0.306、0.376,均P<0.001)。结论4D Auto LAQ可用于评价不同透析方式对尿毒症患者左房结构和功能的影响,其中血液透析较腹膜透析对左房结构和功能影响更大。

二十一世纪以来美国科技政策主题分析及发展态势研判

21世纪以来,新一轮科技革命和产业变革加速演进,国际科技竞争尤其是科技强国大国间的科技竞争日益加剧。美国是国际科技竞争的主要推手,分析21世纪以来美国科技政策主题演变特点,有助于观察美国科技政策发展规律和战略重点。本研究以美国21世纪以来宏观科技政策作为研究对象,按照总统任期划分时间阶段,采用嵌入式主题模型(embedded topic model,ETM)对科技政策文本进行主题发现和分析,结合主题相似度,以可视化方式展现美国科技政策的主题演变过程,观察和分析美国科技政策演变特点及其聚焦的科技战略重点及其启示,以前瞻其未来科技发展趋向。分析结果表明,美国长期重视STEM(science,technology,engineering,mathematics)科技人才教育培养,稳定支持医疗卫生和生物科技领域,聚焦发展信息科技等关键前沿领域,注重能源和生态环境科技领域创新,前瞻布局国家战略性科技产业领域。

基于区间随机阶矩的不确定量化方法

针对缺乏足够信息构建精确概率模型的齿轮—转子机构系统固有特性量化分析问题,提出基于区间随机阶矩技术的不确定性量化方法。引入瑞利商公式,推导了含随机区间混合变量的齿轮—转子系统固有频率的近似解;将区间变量表示为随机变量分布参数的形式,以随机变量进行不确定性建模,并结合多维区间随机函数泰勒展开技术和随机变量函数阶矩法,推导了齿轮—转子系统固有频率的均值和方差的表达式;根据四则运算和区间预算法获得均值和方差的上下界,并通过上下界波动量与函数曲线的凹凸性分析随机和区间变量对系统固有频率的影响规律。最后应用齿轮—转子机构系统数值案例验证了所提方法的正确性和有效性。

基于国家级政策文本计量的科技企业孵化器效果分析

为优化和完善科技企业孵化器政策的顶层设计支持机制提供决策参考,通过收集2006—2022年间我国国家颁布的科技企业孵化器发展的相关政策文件和权威数据,构建效力评估方法与效果评估模型,衡量科技企业孵化器政策效果。结果表明,政策文本数与政策总效力演化趋势基本一致;政策措施、政策目标与政策主体得分情况整体上均优于政策力度;环境型政策工具整体政策效力高于供给型政策工具与需求型政策工具。由此提出未来中国科技企业孵化器政策供给,应重点强化政策供给质量;加强政策力度、政策目标、政策措施、政策主体对政策效果的协同促进作用、匹配成效;巩固环境型政策效果,增加需求型政策工具的供给和使用。

量化式主动呼吸循环技术对食管癌术后患者肺功能及并发症的影响

目的分析基于诱发性肺量计的量化式主动呼吸循环技术对食管癌根治术后患者肺功能及肺部相关并发症的影响。方法选取2021年4月~2023年10月于空军军医大学第二附属医院行食管癌根治术的117例患者,将患者随机分为常规组(n=58,进行缩唇呼吸训练,3次/d)和量化式主动呼吸循环技术(ACBT)组(量化ACBT组)(n=59,使用诱发性肺量计行量化训练)。比较两组患者术前、术后3 d和术后7 d用力肺活量占预计值百分比(FVC%)、第1秒用力呼气量占预计值百分比(FEV1%)、每分钟最大通气量占预计值百分比(MVV%)。比较两组患者术后2、3和5 d痰液清除效果及视觉模拟评分法(VAS)评分,以及术后肺部并发症发生率。结果量化ACBT组患者术后3 d和术后7 d的FVC%、MVV%以及术后7 d的FEV1%均高于常规组患者,差异均有统计学意义(P<0.05)。术后2、3和5 d,量化ACBT组患者的痰液清除优良率均高于常规组患者,差异均有统计学意义(P<0.05)。术后2、3和5 d,量化ACBT组患者的VAS评分略高于常规组,但差异无统计学意义(P>0.05)。量化ACBT组患者的肺部相关并发症发生率为6.77%(4/59),低于常规组患者的18.96%(11/58),差异有统计学意义(χ^(2)=3.885,P=0.048)。结论对于微创食管癌根治术患者,术后采用基于诱发性肺量计的量化主动呼吸循环技术训练有助于排出痰液,改善患者的肺功能,降低胸部并发症的发生率。

全量化工艺处理垃圾填埋场后期及封场渗滤液实例

针对国内垃圾填埋场后期及封场渗滤液可生化性低、无法采用生化进行有效处理、膜浓缩液长期回灌、渗滤液累积盐分越来越高得不到解决等问题,提出一种全量化处理组合工艺,即“软化预处理+高压碟管式反渗透(DTRO)+特种分离膜+低温负压蒸发技术+三相固化技术”,适合盐分高、氨氮高、生化性低的终端渗滤液处理。运行结果表明,清水回收率可达到90%~95%,固化后填埋物仅为5%~10%。液相和固相处理系统优势互补,系统运行稳定,能耗较低,脱盐率达到99.5%以上;出水COD≤60 mg/L,BOD5≤20 mg/L,氨氮≤8 mg/L,总氮≤20 mg/L,SS≤6 mg/L,产水符合《生活垃圾填埋场污染控制标准》(GB 16889—2008)表3规定的限值要求。

A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum

Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.

Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method

Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods.Herein,we used a new strategy:data-dependent acquisition mode identification combined with label-free quantification by SWATH acquisition mode,to study the differentially expressed proteins in mouse liver cancer metastasis cells.A total of 1528 protein groups were identified,among which 1159 protein groups were quantified and 249 protein groups were observed as differentially expressed proteins(86 proteins up-regulated and 163 down-regulated).This method provides a commendable solution for the identification and quantification of differentially expressed proteins in biological samples.

土壤孔隙结构检测技术研究现状与展望

土壤孔隙是水气运移和生物运动的通道,在调节土壤肥力和生态功能中起着至关重要的作用。土壤孔隙结构的量化研究是认识土壤结构、明晰孔隙结构与宏观功能关系的前提。然而,受土壤自身复杂性的限制,目前土壤孔隙结构检测技术普遍存在精确度低、重复性差和操作复杂等问题。针对获取和量化土壤孔隙结构中的重点和难点,归纳了现有方法的优缺点、潜在误差源及应用场景。现有方法总体可分为两大类:间接法和直接观察法,间接法是利用土壤中水、气、声、热等特性获得孔隙直径、体积或孔固体表面积等信息,主要包括水分特征曲线法、压汞法、气体吸附法、声波法、热脉冲-时域反射法、变压法、回归分析法等;直接观察法可以通过几何可视化来直接观察土壤孔隙空间,并借助图像处理技术,从图像中提取大量的定量形态学和拓扑描述符进行评估,例如切片观察法、扫描电子显微镜技术、计算机断层扫描技术、核磁共振技术等。对表征土壤孔隙结构的定量化方法进行了总结,梳理了近5年常规统计法、地统计学和分形理论在土壤孔隙结构定量化研究上的应用和成果。最后对今后田间原位土壤孔隙结构检测的发展方向进行了展望,尝试为土壤孔隙结构的现场检测装置研发提供理论参考和新思路。

流域水文模型参数不确定性量化技术研究进展

流域水文模型参数存在不确定性,不确定性对模型模拟与预测的精度产生显著影响。如何科学量化参数不确定性并分析其产生的不良影响是水文模拟中亟待解决的重要难题。为了研究这一问题,回顾和归纳了不确定性量化研究领域涉及的方法与技术,讨论了不确定性研究各环节中的成熟成果与亟待解决的问题。最后,对流域水文模型参数不确定性量化技术的应用前景进行了展望。