Agricultural contamination with pesticides is a challenging problem for both human and bee health. Consequently, a number of laboratories performing pesticides residues analyse in honey and honey products has graduall...Agricultural contamination with pesticides is a challenging problem for both human and bee health. Consequently, a number of laboratories performing pesticides residues analyse in honey and honey products has gradually increased in recent years. In the framework of their accreditations according to ISO 17025 standard, these laboratories must control their performances through interlaboratory comparison. Interprofessional Bureau of Analytical Studies (BIPEA) organizes regular proficiency testing schemes (PTS) in many analytical domains, including the analysis of pesticides in honey, to compare the performances of the laboratories. As an example, one test was conducted in October 2015 using an organic honey spiked with 21 pesticides, at levels between 15 ^g/kg and 200 ^g/kg, with 27 participating laboratories. This test was intended for the identification and quantification of 21 molecules of pesticides residues. The techniques used by the laboratories were GC-MS-MS, LC-MS-MS, GC-MS and GC-ECD, according to the molecules. Participating laboratories were required to return their results on a dedicated website after a period of one month, and a statistical treatment of the data was performed according to ISO 13528 standard. Assigned (consensus) values were calculated from the participants’ results and the performances of the laboratories could then be evaluated individually and collectively according to ISO 17043 standard. The results of this test were satisfactory for detection and quantification, whatever the analytical technique. The molecules were correctly identified by the laboratories and the recovery rates ranged from 56% up to 88%. The dispersions of the results, studied through the coefficients of variation, were also satisfactory ranging from 7% to 30%. This test showed that the laboratories participating routinely in this kind of tests are very competent in the analysis of pesticides residues in honey. It also allowed the participating laboratories to draw up a general inventory of their analytical skills, and it was a very useful tool to detect bias or non-compliant results. It acted as a warning signal for the implementation of corrective and/or curative actions in the laboratories.展开更多
目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核...目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核酸检测实验室质量控制,重点分析HACCP体系在核酸标本接收、准备试剂、提取标本核酸、核酸扩增、结果判读、核酸检测结果报告环节制定的监控程序、验证措施以及纠偏措施等重要控制点。结果通过分析2021年12月-2022年12月的检测数据,结果显示,各批次核酸检测阳性质控品均满足判定规则,每一批次的实验均处于在控状态。对HACCP体系下实验室数据进行统计,计算其标准差、均值以及变异系数,对箱型图绘制后,展开离群值检验,结果发现,均值没有产生离群值。结论核酸检测实验室中应用HACCP体系,能够有效控制核酸检测实验室质量,全面掌控核酸检测实验室重要控制点,实现实验室生物安全水平与质量的提升,保证核酸检测实验室质控的有效性与科学性,继而有效控制核酸检测实验室质量。展开更多
文摘Agricultural contamination with pesticides is a challenging problem for both human and bee health. Consequently, a number of laboratories performing pesticides residues analyse in honey and honey products has gradually increased in recent years. In the framework of their accreditations according to ISO 17025 standard, these laboratories must control their performances through interlaboratory comparison. Interprofessional Bureau of Analytical Studies (BIPEA) organizes regular proficiency testing schemes (PTS) in many analytical domains, including the analysis of pesticides in honey, to compare the performances of the laboratories. As an example, one test was conducted in October 2015 using an organic honey spiked with 21 pesticides, at levels between 15 ^g/kg and 200 ^g/kg, with 27 participating laboratories. This test was intended for the identification and quantification of 21 molecules of pesticides residues. The techniques used by the laboratories were GC-MS-MS, LC-MS-MS, GC-MS and GC-ECD, according to the molecules. Participating laboratories were required to return their results on a dedicated website after a period of one month, and a statistical treatment of the data was performed according to ISO 13528 standard. Assigned (consensus) values were calculated from the participants’ results and the performances of the laboratories could then be evaluated individually and collectively according to ISO 17043 standard. The results of this test were satisfactory for detection and quantification, whatever the analytical technique. The molecules were correctly identified by the laboratories and the recovery rates ranged from 56% up to 88%. The dispersions of the results, studied through the coefficients of variation, were also satisfactory ranging from 7% to 30%. This test showed that the laboratories participating routinely in this kind of tests are very competent in the analysis of pesticides residues in honey. It also allowed the participating laboratories to draw up a general inventory of their analytical skills, and it was a very useful tool to detect bias or non-compliant results. It acted as a warning signal for the implementation of corrective and/or curative actions in the laboratories.
文摘目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核酸检测实验室质量控制,重点分析HACCP体系在核酸标本接收、准备试剂、提取标本核酸、核酸扩增、结果判读、核酸检测结果报告环节制定的监控程序、验证措施以及纠偏措施等重要控制点。结果通过分析2021年12月-2022年12月的检测数据,结果显示,各批次核酸检测阳性质控品均满足判定规则,每一批次的实验均处于在控状态。对HACCP体系下实验室数据进行统计,计算其标准差、均值以及变异系数,对箱型图绘制后,展开离群值检验,结果发现,均值没有产生离群值。结论核酸检测实验室中应用HACCP体系,能够有效控制核酸检测实验室质量,全面掌控核酸检测实验室重要控制点,实现实验室生物安全水平与质量的提升,保证核酸检测实验室质控的有效性与科学性,继而有效控制核酸检测实验室质量。
