The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variat...The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.展开更多
[Objective] To optimize solid-state fermentation of South China 5 （SC5） cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation condition...[Objective] To optimize solid-state fermentation of South China 5 （SC5） cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation conditions were optimized. [ Result] The optimum conditions of solid-state fermentation were as follows： cassava and leucaena weight ratio, 7：3; fermentation time, 6 d; kombucha volume, 10 ml; fermentation temperature, 30 ℃; and water volume, 200 ml. The content of crude protein in feed was substantially increased and reached 23.64%. [ Conclusion] The optimized fermentation process greatly improves utilization of the SC5 cassava-leucaena protein feed.展开更多
基金supported by the China Agriculture Research System(CARS-11,Sweetpotato)the National Natural Science Foundation of China(31461143017)
文摘The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.
基金supported by Graduate Research Fund of Tropical Crops Genetic Resources Institute,Chinese Academyof Tropical Agricultural SciencesBreeding of New Varieties of High-Starch,Cold-Resistant and Drought-Resistant Cassava(nyhyzx07-013) Earmarked Fund for Modern Agro-Industry Technology Research System (nycytx-17)
文摘[Objective] To optimize solid-state fermentation of South China 5 （SC5） cassava-leucaena protein feed by kombucha. [Method] The SC5 cassava-leucaena protein feed was fermented by kombucha. The fermentation conditions were optimized. [ Result] The optimum conditions of solid-state fermentation were as follows： cassava and leucaena weight ratio, 7：3; fermentation time, 6 d; kombucha volume, 10 ml; fermentation temperature, 30 ℃; and water volume, 200 ml. The content of crude protein in feed was substantially increased and reached 23.64%. [ Conclusion] The optimized fermentation process greatly improves utilization of the SC5 cassava-leucaena protein feed.
