The egg yolks of birds contain most of the maternally derived materials required for embryo development and are an important factor influencing embryo development and offspring viability.Individual variation in egg-la...The egg yolks of birds contain most of the maternally derived materials required for embryo development and are an important factor influencing embryo development and offspring viability.Individual variation in egg-laying date frequently occurs in passerines inhabiting highly seasonal environments.Females laying in early and late stages of the breeding season encounter different environment temperatures and food conditions,which can affect the levels of metabolities in their bodies,thereby altering the transmission of these materials to the eggs.We test a hypothesis that yolk small molecule compounds of Asian Short-toed Lark(Alaudala cheleensis)could vary between early(mid-May)and late(mid-June)broods.Using the UHPLC-MS/MS method,683 compounds belonging to 21 compound groups are detected in the yolks.The contents of 18 compounds are significantly different between early and late broods.Ten differential compounds are significantly higher in the early laid eggs,among whichγ-aminobutyric acid,creatine,prostaglandins,palmitoleic acid,linoleic acid,and trans linoleic acid are related to low environment temperature response.The eggs laid in late stage exhibit significantly higher levels of 5-L-glutamyl-L-alanine andγ-glutamate-leucine,1,3-dimethyluric acid and mannose,which may be attributed to females in the late group consuming more insects.We suggest conducting a comprehensive investigation to reveal the yolk small molecule compounds mediated maternal effects on offspring phenotypes under varying ecological conditions.展开更多
Background:In high latitude grassland habitats,altricial nestlings hatching in open-cup nests early in the breeding season must cope with cold temperature challenges.Thyroid hormones(triiodothyronine,T_(3)and thyroxin...Background:In high latitude grassland habitats,altricial nestlings hatching in open-cup nests early in the breeding season must cope with cold temperature challenges.Thyroid hormones(triiodothyronine,T_(3)and thyroxine,T_(4))and corticosterone play a crucial role in avian thermoregulation response to cold.Investigating the endocrine response of altricial nestlings to temperature variation is important for understanding the adaptive mechanisms of individual variation in the timing of breeding in birds.Methods:We compared nest temperature,ambient temperature,body temperature,plasma T_(3),T_(4)and corticosterone levels in Asian Short-toed Lark(Alaudala cheleensis)nestlings hatching in the early-,middle-,and late-stages of the breeding season in Hulunbuir grassland,northeast China.Results:Mean nest temperature in the early-,middle-and late-stage groups was-1.85,3.81 and 10.23℃,respectively,for the 3-day-old nestlings,and 6.83,10.41 and 11.81℃,respectively,for the 6-day-old nestlings.The nest temperature significantly correlated with body temperature,plasma T_(3),T_(4)and corticosterone concentrations of nestlings.Body temperature of 3-day-old nestlings in the early and middle groups was significantly lower than that of the late group,but there was no significant difference between the nestlings in the early and middle groups.The T_(4)and T_(3)concentrations and the ratio of T_(3)/T_(4)of both 3-and 6-day-old nestlings in the early-stage group were significantly higher compared to the middle and late groups.The corticosterone levels of 3-day-old nestlings were significantly higher in the early-stage group compared to the middle-and late-stage groups.Conclusion:Nestlings hatching early responded to cold temperature by increasing thyroid hormones and corticosterone levels even in the early days of post hatching development when the endothermy has not been established.These hormones may play a physiological role in neonatal nestlings coping with cold temperature challenges.展开更多
语料库译者风格研究已经成为一种新的翻译学研究范式,但基于语料库的诗人译者的风格研究仍处于初始阶段。本文以雪莱诗歌To a Sky-lark的汉译为例,选取郭沫若、查良铮和江枫的三个汉译本,采用定性分析和定量分析相结合的方法,通过自建...语料库译者风格研究已经成为一种新的翻译学研究范式,但基于语料库的诗人译者的风格研究仍处于初始阶段。本文以雪莱诗歌To a Sky-lark的汉译为例,选取郭沫若、查良铮和江枫的三个汉译本,采用定性分析和定量分析相结合的方法,通过自建平行语料库和类比语料库,从词汇层面、句法层面以及主题意象等三个维度着手,进行译者翻译风格的比较研究,以期拓宽语料库译者风格研究的视野,为诗歌翻译、译者风格等相关研究提供一定参考。展开更多
The Song of the Lark tells the story of young girl Thea who doesn’t give up and achieves her wish when faced with all sorts of setbacks. Based on the Marxist feminism, this paper is going to study the differences tha...The Song of the Lark tells the story of young girl Thea who doesn’t give up and achieves her wish when faced with all sorts of setbacks. Based on the Marxist feminism, this paper is going to study the differences that the female and male characters have experienced both in family and society in order to find out the root of females’oppressed status and reveal Cather’s writing in? tention—provide a possible way of improving the dilemma of women caused by capitalist ideology and the patriarchal ideology.展开更多
Background:Timing of breeding season of temperate passerines has been considered to be adjusted to their food availability.There is little work to reveal the cell stress responses of the nestlings hatched asynchronize...Background:Timing of breeding season of temperate passerines has been considered to be adjusted to their food availability.There is little work to reveal the cell stress responses of the nestlings hatched asynchronized with the food abundance peak,which is important for understanding the physiological link between the timing of breeding and the fitness of offspring.Methods:Using gene expression level of blood HSP70 and HSP90 as indicators,we compared the cell stress response of Asian Short-toed Lark(Calandrella cheleensis)nestlings hatched under conditions of low,mid or high food(grasshopper nymph)availability in 2017.Results:Nymph biomass,sample time and interaction of these two factors significantly influenced the blood gene expression level of HSP70 and HSP90 of Asian Short-toed Lark nestlings.HSP70 and HSP90 gene expression levels of the nestlings at 14:00 were significantly higher than those at 5:00.At either 5:00 or 14:00,the gene expression levels of HSP70 and HSP90 increase with the decrease of nymph biomass.Conclusions:These results indicate that food availability is an important environment factor inducing cellular stress of Asian Short-toed Lark nestlings.The interactive effect of the nymph abundance and sample time on the HSPs response may be related with the daily temperature variation of the grassland.Over cell stress response may be one of physiological factor mediating the effect of food availability and the nestling’s fitness.展开更多
Background:Predicting the possibility of severe effects of global warming on animals is important for understanding the ecological consequences of climate change on ecosystem.Spring is the season during which birds ha...Background:Predicting the possibility of severe effects of global warming on animals is important for understanding the ecological consequences of climate change on ecosystem.Spring is the season during which birds have to physiologically prepare for the subsequent breeding period,and unusual spring temperature rising probably becomes a heat stress to the birds which have adapted to the low spring temperature.Therefore,it is necessary to understand the physiological effect of spring warming on the temperate birds.Methods:Using the activities of blood anti-oxidative enzymes(SOD,CAT,GPx)and the concentrations of serum immunogloblins(IgA,IgY,IgM)as indicators,we compared the anti-oxidative and immune functions of Asian Shorttoed Larks(Calandrella cheleensis)captured between 10 and 15 March,2015 and housed under conditions of 21°C and 16°C.Results:The SOD activities of birds in 21°C group were significantly lower than those in 16°C group on all the treatment days.The CAT activities of the birds in 21°C group were significantly lower than those in 16°C group on the 1 st,5 th,13 th,17 treatment days.The GPx activities of the birds in 21°C group were signifthicantly lower than those in 16°C group on the 1 st,13 th and 17 th,but significantly higher on the 21 st treatment day.The IgA,IgY and IgM concentrations of birds in 21°C group were significantly lower than those in 16°C group on all the treatment days.Conclusions:This study shows that spring temperature rising negatively influences antioxibative and humoral immune functions,which indicates that spring climate warming might reduce the fitness of the temperate passerine birds which have adapted to the low spring temperature.展开更多
《达洛维夫人》是弗吉尼亚·伍尔夫最有影响力的一部小说。文章开头第三段的两个感叹句"What a lark!What a plunge!"表达了作者和主人公对生命和现实的深入思考。lark象征着幸福和达洛维夫人的内心世界,plunge则象征着...《达洛维夫人》是弗吉尼亚·伍尔夫最有影响力的一部小说。文章开头第三段的两个感叹句"What a lark!What a plunge!"表达了作者和主人公对生命和现实的深入思考。lark象征着幸福和达洛维夫人的内心世界,plunge则象征着现实的生活和她平淡婚姻的痛苦。展开更多
The family Alaudidae,larks,comprises 93-100 species(depending on taxonomy)that are widely distributed across Africa and Eurasia,with single species extending their ranges to North and northernmost South America and Au...The family Alaudidae,larks,comprises 93-100 species(depending on taxonomy)that are widely distributed across Africa and Eurasia,with single species extending their ranges to North and northernmost South America and Australia.A decade-old molecular phylogeny,comprising~80%of the species,revealed multiple cases of parallel evolution and large variation in rates of morphological evolution,which had misled taxonomists into creating many non-monophyletic genera.Here,we reconstruct the phylogeny of the larks,using a dataset covering one mitochondrial and 16 nuclear loci and comprising all except one of the currently recognised species as well as several recently proposed new species(in total 133 taxa;not all loci available for all species).We provide additional support using genome-wide markers to infer a genus-level phylogeny based on near-complete generic sampling(in total 51 samples of 44 taxa across 40 species).Our results confirm the previous findings of rampant morphological convergence and divergence,and reveal new cases of paraphyletic genera.We propose a new subfamily classification,and also that the genus Mirafra is divided into four genera to produce a more balanced generic classification of the Alaudidae.Our study supports recently proposed species splits as well as some recent lumps,while also questioning some of the latter.This comprehensive phylogeny will form an important basis for future studies,such as comparative studies of lark natural history,ecology,evolution and conservation.展开更多
William Ernest Henley is a famous poet and suffers from tuberculosis seriously.In A Late Lark Twitters,he draws a comparison between the description of a scenery at sunset which is accompanied by the singing of a lark...William Ernest Henley is a famous poet and suffers from tuberculosis seriously.In A Late Lark Twitters,he draws a comparison between the description of a scenery at sunset which is accompanied by the singing of a lark and the then formulated wish to die in the very same way,to leave the earth at the end of life like the sun at the end of day.展开更多
The incubating behavior of the horned lark and small skylark was studied in alpine meadows. The key results are: ① There was no significant difference in the temperature outside the nests of horned larks and small sk...The incubating behavior of the horned lark and small skylark was studied in alpine meadows. The key results are: ① There was no significant difference in the temperature outside the nests of horned larks and small skylarks ( F 1, 87 =3 286,P >0 05). ② If parents were off the nest, the temperature in the nest was not significantly different between the two species( F 1,87 =3 009,P >0 05), however, if parent birds were on the nest, the temperature in horned lark nests was significantly higher than that in small lark nests ( F 1, 210 =7 555,P <0 01). ③ Temperature trends inside and outside the nests of the two species were similar, both following a low high low, pattern and showed a significantly positive correlation(horned lark: r=0 572, n=158, P <0 01;small skylark: r=0 511, n=141, P <0 01). ④ During the hatching period, the temperature in nests was significantly higher than the outside temperature in horned larks( t=7 051, df=157, P <0 01), however, there was no significant difference between the inside and outside in the small skylark( t=-1 558, df=149, P >0 05). ⑤ The attendance time of horned larks and small skylarks showed three peaks; before 09:00 h, between 12:00 and 15:00 and after 19:00 h, respectively. ⑥ The attendance time and incubation behavior of the two species changed with variation in the environmental temperature. ⑦ There was no significant difference in the average attendance time per day between horned larks and small skylarks( P >0 05). ⑧ The attendance frequency of horned larks was high (4 17 times/h), and visit duration short (524 32 s/period), wherase small larks showed the opposite pattern; low attendance frequency(1 79 times/h)with relatively long( 1 510 00 s/ periods)visits. Our results are consistent with the hypothesis that attendance time is a balance between the energy requirements of the parent birds and the temperature requirements of the developing embryos. It follows that a birds choice of nest site should involve a trade off between costs and benefits, e.g. microclimate versus proximity to food resources.展开更多
基金supported by the National Natural Science Foundation of China(No.32071515 to SZ)Graduate Research and Practice Projects of Minzu University of China(BZKY2022042).
文摘The egg yolks of birds contain most of the maternally derived materials required for embryo development and are an important factor influencing embryo development and offspring viability.Individual variation in egg-laying date frequently occurs in passerines inhabiting highly seasonal environments.Females laying in early and late stages of the breeding season encounter different environment temperatures and food conditions,which can affect the levels of metabolities in their bodies,thereby altering the transmission of these materials to the eggs.We test a hypothesis that yolk small molecule compounds of Asian Short-toed Lark(Alaudala cheleensis)could vary between early(mid-May)and late(mid-June)broods.Using the UHPLC-MS/MS method,683 compounds belonging to 21 compound groups are detected in the yolks.The contents of 18 compounds are significantly different between early and late broods.Ten differential compounds are significantly higher in the early laid eggs,among whichγ-aminobutyric acid,creatine,prostaglandins,palmitoleic acid,linoleic acid,and trans linoleic acid are related to low environment temperature response.The eggs laid in late stage exhibit significantly higher levels of 5-L-glutamyl-L-alanine andγ-glutamate-leucine,1,3-dimethyluric acid and mannose,which may be attributed to females in the late group consuming more insects.We suggest conducting a comprehensive investigation to reveal the yolk small molecule compounds mediated maternal effects on offspring phenotypes under varying ecological conditions.
基金supported by the National Natural Science Foundation of China(No.32071515)。
文摘Background:In high latitude grassland habitats,altricial nestlings hatching in open-cup nests early in the breeding season must cope with cold temperature challenges.Thyroid hormones(triiodothyronine,T_(3)and thyroxine,T_(4))and corticosterone play a crucial role in avian thermoregulation response to cold.Investigating the endocrine response of altricial nestlings to temperature variation is important for understanding the adaptive mechanisms of individual variation in the timing of breeding in birds.Methods:We compared nest temperature,ambient temperature,body temperature,plasma T_(3),T_(4)and corticosterone levels in Asian Short-toed Lark(Alaudala cheleensis)nestlings hatching in the early-,middle-,and late-stages of the breeding season in Hulunbuir grassland,northeast China.Results:Mean nest temperature in the early-,middle-and late-stage groups was-1.85,3.81 and 10.23℃,respectively,for the 3-day-old nestlings,and 6.83,10.41 and 11.81℃,respectively,for the 6-day-old nestlings.The nest temperature significantly correlated with body temperature,plasma T_(3),T_(4)and corticosterone concentrations of nestlings.Body temperature of 3-day-old nestlings in the early and middle groups was significantly lower than that of the late group,but there was no significant difference between the nestlings in the early and middle groups.The T_(4)and T_(3)concentrations and the ratio of T_(3)/T_(4)of both 3-and 6-day-old nestlings in the early-stage group were significantly higher compared to the middle and late groups.The corticosterone levels of 3-day-old nestlings were significantly higher in the early-stage group compared to the middle-and late-stage groups.Conclusion:Nestlings hatching early responded to cold temperature by increasing thyroid hormones and corticosterone levels even in the early days of post hatching development when the endothermy has not been established.These hormones may play a physiological role in neonatal nestlings coping with cold temperature challenges.
文摘语料库译者风格研究已经成为一种新的翻译学研究范式,但基于语料库的诗人译者的风格研究仍处于初始阶段。本文以雪莱诗歌To a Sky-lark的汉译为例,选取郭沫若、查良铮和江枫的三个汉译本,采用定性分析和定量分析相结合的方法,通过自建平行语料库和类比语料库,从词汇层面、句法层面以及主题意象等三个维度着手,进行译者翻译风格的比较研究,以期拓宽语料库译者风格研究的视野,为诗歌翻译、译者风格等相关研究提供一定参考。
文摘The Song of the Lark tells the story of young girl Thea who doesn’t give up and achieves her wish when faced with all sorts of setbacks. Based on the Marxist feminism, this paper is going to study the differences that the female and male characters have experienced both in family and society in order to find out the root of females’oppressed status and reveal Cather’s writing in? tention—provide a possible way of improving the dilemma of women caused by capitalist ideology and the patriarchal ideology.
基金supported by the National Natural Science Foundation of China(No.31872246)
文摘Background:Timing of breeding season of temperate passerines has been considered to be adjusted to their food availability.There is little work to reveal the cell stress responses of the nestlings hatched asynchronized with the food abundance peak,which is important for understanding the physiological link between the timing of breeding and the fitness of offspring.Methods:Using gene expression level of blood HSP70 and HSP90 as indicators,we compared the cell stress response of Asian Short-toed Lark(Calandrella cheleensis)nestlings hatched under conditions of low,mid or high food(grasshopper nymph)availability in 2017.Results:Nymph biomass,sample time and interaction of these two factors significantly influenced the blood gene expression level of HSP70 and HSP90 of Asian Short-toed Lark nestlings.HSP70 and HSP90 gene expression levels of the nestlings at 14:00 were significantly higher than those at 5:00.At either 5:00 or 14:00,the gene expression levels of HSP70 and HSP90 increase with the decrease of nymph biomass.Conclusions:These results indicate that food availability is an important environment factor inducing cellular stress of Asian Short-toed Lark nestlings.The interactive effect of the nymph abundance and sample time on the HSPs response may be related with the daily temperature variation of the grassland.Over cell stress response may be one of physiological factor mediating the effect of food availability and the nestling’s fitness.
基金supported by the National Natural Science Foundation of China(No.31872246)。
文摘Background:Predicting the possibility of severe effects of global warming on animals is important for understanding the ecological consequences of climate change on ecosystem.Spring is the season during which birds have to physiologically prepare for the subsequent breeding period,and unusual spring temperature rising probably becomes a heat stress to the birds which have adapted to the low spring temperature.Therefore,it is necessary to understand the physiological effect of spring warming on the temperate birds.Methods:Using the activities of blood anti-oxidative enzymes(SOD,CAT,GPx)and the concentrations of serum immunogloblins(IgA,IgY,IgM)as indicators,we compared the anti-oxidative and immune functions of Asian Shorttoed Larks(Calandrella cheleensis)captured between 10 and 15 March,2015 and housed under conditions of 21°C and 16°C.Results:The SOD activities of birds in 21°C group were significantly lower than those in 16°C group on all the treatment days.The CAT activities of the birds in 21°C group were significantly lower than those in 16°C group on the 1 st,5 th,13 th,17 treatment days.The GPx activities of the birds in 21°C group were signifthicantly lower than those in 16°C group on the 1 st,13 th and 17 th,but significantly higher on the 21 st treatment day.The IgA,IgY and IgM concentrations of birds in 21°C group were significantly lower than those in 16°C group on all the treatment days.Conclusions:This study shows that spring temperature rising negatively influences antioxibative and humoral immune functions,which indicates that spring climate warming might reduce the fitness of the temperate passerine birds which have adapted to the low spring temperature.
基金the National Swedish Research Council(grants No.2015-04402,2019-04486)the Carl Trygger Foundation(CTS 20:6)+3 种基金the Jornvall FoundationJulian Francis for financial supportthe National Genomics Infrastructure in Stockholm funded by Science for Life Laboratory,the Knut and Alice Wallenberg Foundationthe Research/Scientific Computing teams at The James Hutton Institute and NIAB for providing computational resources and technical support for the"UK's Crop Diversity Bioinformatics HPC"(BBSRC grant BB/S019669/1)。
文摘The family Alaudidae,larks,comprises 93-100 species(depending on taxonomy)that are widely distributed across Africa and Eurasia,with single species extending their ranges to North and northernmost South America and Australia.A decade-old molecular phylogeny,comprising~80%of the species,revealed multiple cases of parallel evolution and large variation in rates of morphological evolution,which had misled taxonomists into creating many non-monophyletic genera.Here,we reconstruct the phylogeny of the larks,using a dataset covering one mitochondrial and 16 nuclear loci and comprising all except one of the currently recognised species as well as several recently proposed new species(in total 133 taxa;not all loci available for all species).We provide additional support using genome-wide markers to infer a genus-level phylogeny based on near-complete generic sampling(in total 51 samples of 44 taxa across 40 species).Our results confirm the previous findings of rampant morphological convergence and divergence,and reveal new cases of paraphyletic genera.We propose a new subfamily classification,and also that the genus Mirafra is divided into four genera to produce a more balanced generic classification of the Alaudidae.Our study supports recently proposed species splits as well as some recent lumps,while also questioning some of the latter.This comprehensive phylogeny will form an important basis for future studies,such as comparative studies of lark natural history,ecology,evolution and conservation.
文摘William Ernest Henley is a famous poet and suffers from tuberculosis seriously.In A Late Lark Twitters,he draws a comparison between the description of a scenery at sunset which is accompanied by the singing of a lark and the then formulated wish to die in the very same way,to leave the earth at the end of life like the sun at the end of day.
文摘The incubating behavior of the horned lark and small skylark was studied in alpine meadows. The key results are: ① There was no significant difference in the temperature outside the nests of horned larks and small skylarks ( F 1, 87 =3 286,P >0 05). ② If parents were off the nest, the temperature in the nest was not significantly different between the two species( F 1,87 =3 009,P >0 05), however, if parent birds were on the nest, the temperature in horned lark nests was significantly higher than that in small lark nests ( F 1, 210 =7 555,P <0 01). ③ Temperature trends inside and outside the nests of the two species were similar, both following a low high low, pattern and showed a significantly positive correlation(horned lark: r=0 572, n=158, P <0 01;small skylark: r=0 511, n=141, P <0 01). ④ During the hatching period, the temperature in nests was significantly higher than the outside temperature in horned larks( t=7 051, df=157, P <0 01), however, there was no significant difference between the inside and outside in the small skylark( t=-1 558, df=149, P >0 05). ⑤ The attendance time of horned larks and small skylarks showed three peaks; before 09:00 h, between 12:00 and 15:00 and after 19:00 h, respectively. ⑥ The attendance time and incubation behavior of the two species changed with variation in the environmental temperature. ⑦ There was no significant difference in the average attendance time per day between horned larks and small skylarks( P >0 05). ⑧ The attendance frequency of horned larks was high (4 17 times/h), and visit duration short (524 32 s/period), wherase small larks showed the opposite pattern; low attendance frequency(1 79 times/h)with relatively long( 1 510 00 s/ periods)visits. Our results are consistent with the hypothesis that attendance time is a balance between the energy requirements of the parent birds and the temperature requirements of the developing embryos. It follows that a birds choice of nest site should involve a trade off between costs and benefits, e.g. microclimate versus proximity to food resources.