The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling p...The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling pathways may be associated with limb regeneration and development in crustaceans.However,the detail mechanisms remain unclear.Therefore,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western blotting.Signifi cant PtWNT4 expression was detected in heart and eyestalk.In addition,PtWNT4 was expressed in all larval stages of P.trituberculatus with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace area.The injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene silencing.Overall,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in P.trituberculatus.The results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.展开更多
The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 w...The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.展开更多
The mRNA differential display (DDRT-PCR) technique was adopted to find out the genes related tosettlement metamorphosis development process of Ruditapes philippinarum larvae.In this study,we haveobtained three hundred...The mRNA differential display (DDRT-PCR) technique was adopted to find out the genes related tosettlement metamorphosis development process of Ruditapes philippinarum larvae.In this study,we haveobtained three hundred and forty-six amplification bands in total from pediveliger larvae,veliger larvae,eye spot larvae and post-larvae.Sixty-five out of three hundred and forty-six bands are distinctly differen-tial display from band pattern,which can be put into four groups,standing for different expression char-acters.Sixteen differential display bands were cloned,sequenced and analyzed and nine different se-quences are obtained in the study.Three sequences have higher similarity to the cDNAs deposited indatabase and three are very similar to the rDNA of other species,considered as the rDNA of Ruditapesphilippinarum.The rest three sequences are found to be novel sequences after analyzed.Their accessionnumbers are AY916799,AY916798,and AY916797 respectively.We thought the novel sequences arepossibly relevant to the early embryo development of Ruditapes philippinarum larvae and can provide somefundamental understandings that are helpful for the improvement of scallop seed raising industry.展开更多
In order to understand the occurrence and development of Semanotus bifasciatus （Motschulsky）, a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the develo...In order to understand the occurrence and development of Semanotus bifasciatus （Motschulsky）, a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the developmental threshold temperature （C1） and effective accumulated temperature （K） of S. bifasciatus were determined under conditions of constant and variable temperatures. The results show that under constant temperatures the value of C1 has a range of 8.90±1.77℃and the value of K lies between 95.19±13.14 degree-day for eggs; the values for larvae are C1= 13.26±3.06℃ and the number of degree-day is K 2,885.07±187.87 degree-day; for pupae the ranges are C1 = 8.93±2.49℃ and K= 131.20±25.63 degree-day. Under the condition of ambient temperatures, the values for eggs are C1= 17.33±1.24℃ and K= 70.79±8.99 degree-day. It is suggested that S. bifasciatus adults over-winter in a state of dormancy, not at a diapause. Warm winter may accelerate the life cycle ofS. bifasciatus. Equations can be used to predict an early occurrence ofS. bifasciatus.展开更多
Ecological experiment was conducted in this paper to study the allometric growth in Squaliobarbus curriculus larvae and juveniles from Zhaoqing Reach of the Pearl River. The results showed that after first feeding, th...Ecological experiment was conducted in this paper to study the allometric growth in Squaliobarbus curriculus larvae and juveniles from Zhaoqing Reach of the Pearl River. The results showed that after first feeding, the organs associated to swimming, feeling and feeding of larvae differentiated rapidly, and exhibited allometric growth. The head length, tail length and head height exhibited positive allometric growth, while trunk length exhibited negative allometric growth before reaching the growth inflexion point 20 d after first feeding, and positive allometric growth after the inflection point. Body height exhibited positive allometric growth both before and after the growth inflexion point at 18 d after first feeding. The inflexion points of snout length, eye diameter and post-eye head length occurred 23, 19 and 16 d after first feeding. For swimming organs, the dorsal fin, pelvic fins and anal fin exhibited positive allometric growth both before and after the inflexion point at 18, 19 and 17 d after first feeding; but the tail fin exhibited positive allometric growth before the inflexion point at 14 d after first feeding, and negative allometric growth after the inflexion point. S. curriculus larvae can possess various abilities soon after first feeding to survive at early stage due to the rapid development of swimming, feeling, feeding and other organs, which has important ecological significance to adapt to the complex external environment.展开更多
基金Supported by the National Natural Science Foundation of China(No.31602152)the Major Agriculture Program of Ningbo(No.2017C110007)the K.C.Wong Magana Fund in Ningbo University.The funding body had no role in the study design,experimental implementation,interpretation of data,or writing of the manuscript。
文摘The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling pathways may be associated with limb regeneration and development in crustaceans.However,the detail mechanisms remain unclear.Therefore,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western blotting.Signifi cant PtWNT4 expression was detected in heart and eyestalk.In addition,PtWNT4 was expressed in all larval stages of P.trituberculatus with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace area.The injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene silencing.Overall,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in P.trituberculatus.The results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.
基金supported by National Basic Research Program of China(2010CB834202)the National Natural Science Foundation of China(11305226,11175222)+1 种基金the Scientific Technology Research Projects of Lanzhou City(2015-3-75)the Western Talent Program of Chinese Academy of Sciences(Y460030XB0)
文摘The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.
基金the National High Technology Research and Development Programme of China(No.2002AA603015)
文摘The mRNA differential display (DDRT-PCR) technique was adopted to find out the genes related tosettlement metamorphosis development process of Ruditapes philippinarum larvae.In this study,we haveobtained three hundred and forty-six amplification bands in total from pediveliger larvae,veliger larvae,eye spot larvae and post-larvae.Sixty-five out of three hundred and forty-six bands are distinctly differen-tial display from band pattern,which can be put into four groups,standing for different expression char-acters.Sixteen differential display bands were cloned,sequenced and analyzed and nine different se-quences are obtained in the study.Three sequences have higher similarity to the cDNAs deposited indatabase and three are very similar to the rDNA of other species,considered as the rDNA of Ruditapesphilippinarum.The rest three sequences are found to be novel sequences after analyzed.Their accessionnumbers are AY916799,AY916798,and AY916797 respectively.We thought the novel sequences arepossibly relevant to the early embryo development of Ruditapes philippinarum larvae and can provide somefundamental understandings that are helpful for the improvement of scallop seed raising industry.
文摘In order to understand the occurrence and development of Semanotus bifasciatus （Motschulsky）, a borer insect of Platycladus orientalis in parts of northern and eastern China and to improve its prediction, the developmental threshold temperature （C1） and effective accumulated temperature （K） of S. bifasciatus were determined under conditions of constant and variable temperatures. The results show that under constant temperatures the value of C1 has a range of 8.90±1.77℃and the value of K lies between 95.19±13.14 degree-day for eggs; the values for larvae are C1= 13.26±3.06℃ and the number of degree-day is K 2,885.07±187.87 degree-day; for pupae the ranges are C1 = 8.93±2.49℃ and K= 131.20±25.63 degree-day. Under the condition of ambient temperatures, the values for eggs are C1= 17.33±1.24℃ and K= 70.79±8.99 degree-day. It is suggested that S. bifasciatus adults over-winter in a state of dormancy, not at a diapause. Warm winter may accelerate the life cycle ofS. bifasciatus. Equations can be used to predict an early occurrence ofS. bifasciatus.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303048)Major Program of Natural Science Foundation of Guangxi(013GXNSFEA053003)
文摘Ecological experiment was conducted in this paper to study the allometric growth in Squaliobarbus curriculus larvae and juveniles from Zhaoqing Reach of the Pearl River. The results showed that after first feeding, the organs associated to swimming, feeling and feeding of larvae differentiated rapidly, and exhibited allometric growth. The head length, tail length and head height exhibited positive allometric growth, while trunk length exhibited negative allometric growth before reaching the growth inflexion point 20 d after first feeding, and positive allometric growth after the inflection point. Body height exhibited positive allometric growth both before and after the growth inflexion point at 18 d after first feeding. The inflexion points of snout length, eye diameter and post-eye head length occurred 23, 19 and 16 d after first feeding. For swimming organs, the dorsal fin, pelvic fins and anal fin exhibited positive allometric growth both before and after the inflexion point at 18, 19 and 17 d after first feeding; but the tail fin exhibited positive allometric growth before the inflexion point at 14 d after first feeding, and negative allometric growth after the inflexion point. S. curriculus larvae can possess various abilities soon after first feeding to survive at early stage due to the rapid development of swimming, feeling, feeding and other organs, which has important ecological significance to adapt to the complex external environment.
