BACKGROUND Collision carcinoma is rare in clinical practice, especially in the head and neck region. In this paper, we report a case of squamous cell carcinoma(SCC) and neuroendocrine carcinoma(NEC) colliding in the l...BACKGROUND Collision carcinoma is rare in clinical practice, especially in the head and neck region. In this paper, we report a case of squamous cell carcinoma(SCC) and neuroendocrine carcinoma(NEC) colliding in the larynx and review 12 cases of collision carcinoma in the head and neck to further understand collision carcinoma, including its definition, diagnosis, and treatment.CASE SUMMARY A 61-year-old man presented with a 1-year history of hoarseness. Contrastenhanced magnetic resonance imaging of the larynx revealed that the right vocal cord had a nodule-like thickening with obvious enhancement. Laryngoscopy revealed a neoplasm on the right vocal cord, and a malignant tumor was initially considered. A frozen section of right vocal cord was performed under general anesthesia. The pathological result showed a malignant tumor in the right vocal cord. The tumor was excised with a CO_2 laser(Vc type). Routine postoperative pathology showed moderately differentiated SCC with small cell NEC in the right vocal cord. No metastatic lymph nodes or distant metastases were found on postoperative positron emission tomography/computed tomography. Because of the coexistence of SCC and NEC, the patient received adjuvant chemotherapy and radiotherapy. The patient was followed for 8 mo, and no recurrence or distant metastasis was found.CONCLUSION The treatment of collision carcinoma in the head and neck region is uncertain due to the small number of cases.展开更多
Summary: The inhibitory effects of two kinds of selective cyclooxygenase-2 inhibitors on the proliferation of human carcinoma of larynx Hep-2 in vitro and their corresponding mechanisms were investigated. Hep-2 cells ...Summary: The inhibitory effects of two kinds of selective cyclooxygenase-2 inhibitors on the proliferation of human carcinoma of larynx Hep-2 in vitro and their corresponding mechanisms were investigated. Hep-2 cells were cultured with two kinds of selective cyclooxygenase-2 inhibitors (Sc-58125 and Celecoxib) at various concentrations for 24 h. Morphological changes were observed under the phase microscopy and the growth suppression was detected by using MTT colorimetric assay. Apoptotic DNA fragments were observed by agarose gel electrophoresis, and the cell cycle and apoptotic rate were detected by flow cytometry (FCM) respectively. Hep-2 cells became rounded and detached from the culture dish after being treated with Celecoxib for 24 h, however, they remained morphologically unchanged with Sc-58125. Sc-58125 could increase G 2 phase cells, whereas, Celecoxib rose G 1 phase cells. Both of the two effects were dose-dependent. Moreover, the Hep-2 cells cultured with 50 μmol/L and 100 μmol/L Celecoxib showed obvious apoptosis, with the nuclear DNA of cells exhibiting characteristic DNA ladder. So Sc-58125 could inhibit the proliferation of Hep-2 cells by altering the G 2 phase cells. However, Celecoxib had the same effect by changing the G 1 phase cells and inducing apoptosis at higher concentration.展开更多
Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collec...Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collected and expressions of Survivin and VEGF in peripheral blood were investigated by enzyme-linked immunosorbent assay （ELISA）. Results： Expressions of Survivin and VEGF were significantly associated with T stage, N stage and metastasis of squamous carcinoma of larynx. The patients with Survivin or VEGF over-expressions presented lower survival rate, respectively, as compared to those of low-expression （P 〈 0.05）. The survival rate in squamous carcinoma of larynx patients with Survivin and VEGF dual over-expressions was significantly lower than that of patients with dual low-expression （P 〈 0.05）. Multivariate analysis indicated that both Survivin and VEGF over-expressions in squamous carcinoma of larynx peripheral blood samples were strong independent factors of poor prognosis in squamous carcinoma of larynx patients. Conclusion： Survivin and VEGF over-expressions are independent prognostic factors for the patients with squamous carcinoma of larynx. These results also suggest that peripheral blood Survivin and VEGF expressions are valuable prognostic markers for prognosis prediction in squamous carcinoma of larynx patients.展开更多
Postoperative radiotherapy (PRT) is widely advocated for patients with squamous cell carcinomas of the head and neck that are considered to be at high risk of recurrence after surgical resection. The aims of this stud...Postoperative radiotherapy (PRT) is widely advocated for patients with squamous cell carcinomas of the head and neck that are considered to be at high risk of recurrence after surgical resection. The aims of this study were to evaluate the treatment outcomes of PRT for patients with laryngeal carcinoma and to identify the value of several prognostic factors. We reviewed the records of 256 patients treated for laryngeal squamous cell carcinoma between January 1993 and December 2005. Disease-free survival (DFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Log-rank test was employed to identify significant prognostic factors for DFS and OS. The Cox proportional hazards model was applied to identify covariates significantly associated with the aforementioned endpoints. Our results showed the 3-, 5-, and 10-year DFS for all patients were 69.9%, 59.5%, and 34.9%, respectively. The 3-, 5-, and 10-year OS rates were 80.8%, 68.6%, and 38.8%, respectively. Significant prognostic factors for both DFS and OS on univariate analysis were grade, primary site, T stage, N stage, overall stage, lymph node metastasis, overall treatment times of radiation, the interval between surgery and radiotherapy, and radiotherapy equipment. Favorable prognostic factors for both DFS and OS on multivariate analysis were lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment. In conclusion, our data suggest that lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment are favorable prognostic factors for DFS and OS and that reducing the overall treatment times of radiation to 6 weeks or less and the interval between surgery and radiotherapy to less than 3 weeks are simple measures to remarkably improve treatment outcome.展开更多
Background: The current treatment options of early glottic carcinoma are radiotherapy;trans laser microsurgery, and open surgery. However, the best treatment is still controversial due to lack of randomized controlled...Background: The current treatment options of early glottic carcinoma are radiotherapy;trans laser microsurgery, and open surgery. However, the best treatment is still controversial due to lack of randomized controlled trials. We aimed to evaluate the treatment results and the prognostic factors of local control of early glottic squamous cell carcinoma patients (GSCC) T1-2N0M0 treated at our institution. Material and Methods: We retrospectively studied the charts of 52 patients with early GSCC T1-T2N0M0 from 2010-2015 at the Clinical Oncology Department, Ain-Shams University. 24 patients had T1 and 28 had T2 early glottic carcinoma. The overall survival OS, local control rate, and laryngeal preservation rate were evaluated. Kaplan-Meier method, Cox proportional hazards model were used to analyze the data. Results: Median duration of follow-up was 13 months. Thirty-eight patients received radiation treatment alone (73.1%), 7.7% of the patients underwent surgery alone, and 19.2% of the patients had surgery combined with radiotherapy. Local recurrence after radiation failure developed in 6/52 patients, all had T2 disease and were salvaged by total laryngectomy. The ultimate local control rate was 88.5%, and the ultimate laryngeal preservation rate was 77.2% (40/52 patients). The median OS of the 52 patients was 13 months (range 2 - 46 months). Univariate analysis of factors associated with poor local control showed that age > 60 years was the only significant factor (P = 0.048). Conclusion: Radiotherapy achieves high local control and laryngeal preservation rates for patients with early glottic carcinoma, and is associated with a low rate of severe complications compared to surgery. Salvage surgery is feasible after radiotherapy failure.展开更多
目的比较低温等离子射频消融术与CO2激光治疗早期声门型喉癌的临床疗效。方法选取2007年10月~2012年12月行手术治疗的104例早期声门型喉癌（Tis、T1a、T1b）患者,其中64例行低温等离子射频消融术（等离子组）,40例行CO2激光切除术（CO2...目的比较低温等离子射频消融术与CO2激光治疗早期声门型喉癌的临床疗效。方法选取2007年10月~2012年12月行手术治疗的104例早期声门型喉癌（Tis、T1a、T1b）患者,其中64例行低温等离子射频消融术（等离子组）,40例行CO2激光切除术（CO2激光组）。记录并比较两组手术时间、术后第二日疼痛程度[视觉模拟评分法（visual analog scale,VAS）]评分;所有患者于术后一周、1个月及3个月行电子喉镜及嗓音声学分析,并进行随访观察。结果等离子组平均手术时间为8.75±1.62min,CO2激光组平均手术时间为11.82±1.51min,等离子组优于CO2激光组（P〈0.05）。术后第二日VAS评分等离子组（2.79±0.47分）与CO2激光组（2.96±0.64分）组间差异无统计学意义（P〉0.05）。等离子组术后1个月粘膜恢复情况好于CO2激光组（χ2=13.842,P〈0.05）。单因素重复测量分析结果显示：等离子组及CO2激光组术后嗓音声学指标jitter、shimmer、HNR随术后时间延长逐渐好转,在术后3个时间点的变化差异有统计学意义（P〈0.05）,且呈线性变化趋势。多因素重复测量方差分析结果显示：嗓音声学指标（jitter、shimmer、HNR）在术后3个时间点两组间差异均有统计学意义（P〈0.05）。等离子组与CO2激光组术后3年生存率、局部复发率、侵及前联合复发率、前联合术后粘连率组间差异均无统计学意义（P〉0.05）。两组所有患者均未行气管切开术,术后无呛咳,无呼吸困难,无严重疼痛及咯血等严重并发症。结论低温等离子射频消融术与CO2激光治疗早期声门型喉癌均较安全、有效,而低温等离子射频消融术手术时间较CO2激光手术短,术后一月粘膜恢复及嗓音恢复均优于CO2激光手术。展开更多
文摘BACKGROUND Collision carcinoma is rare in clinical practice, especially in the head and neck region. In this paper, we report a case of squamous cell carcinoma(SCC) and neuroendocrine carcinoma(NEC) colliding in the larynx and review 12 cases of collision carcinoma in the head and neck to further understand collision carcinoma, including its definition, diagnosis, and treatment.CASE SUMMARY A 61-year-old man presented with a 1-year history of hoarseness. Contrastenhanced magnetic resonance imaging of the larynx revealed that the right vocal cord had a nodule-like thickening with obvious enhancement. Laryngoscopy revealed a neoplasm on the right vocal cord, and a malignant tumor was initially considered. A frozen section of right vocal cord was performed under general anesthesia. The pathological result showed a malignant tumor in the right vocal cord. The tumor was excised with a CO_2 laser(Vc type). Routine postoperative pathology showed moderately differentiated SCC with small cell NEC in the right vocal cord. No metastatic lymph nodes or distant metastases were found on postoperative positron emission tomography/computed tomography. Because of the coexistence of SCC and NEC, the patient received adjuvant chemotherapy and radiotherapy. The patient was followed for 8 mo, and no recurrence or distant metastasis was found.CONCLUSION The treatment of collision carcinoma in the head and neck region is uncertain due to the small number of cases.
基金This project was supported by the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE of China
文摘Summary: The inhibitory effects of two kinds of selective cyclooxygenase-2 inhibitors on the proliferation of human carcinoma of larynx Hep-2 in vitro and their corresponding mechanisms were investigated. Hep-2 cells were cultured with two kinds of selective cyclooxygenase-2 inhibitors (Sc-58125 and Celecoxib) at various concentrations for 24 h. Morphological changes were observed under the phase microscopy and the growth suppression was detected by using MTT colorimetric assay. Apoptotic DNA fragments were observed by agarose gel electrophoresis, and the cell cycle and apoptotic rate were detected by flow cytometry (FCM) respectively. Hep-2 cells became rounded and detached from the culture dish after being treated with Celecoxib for 24 h, however, they remained morphologically unchanged with Sc-58125. Sc-58125 could increase G 2 phase cells, whereas, Celecoxib rose G 1 phase cells. Both of the two effects were dose-dependent. Moreover, the Hep-2 cells cultured with 50 μmol/L and 100 μmol/L Celecoxib showed obvious apoptosis, with the nuclear DNA of cells exhibiting characteristic DNA ladder. So Sc-58125 could inhibit the proliferation of Hep-2 cells by altering the G 2 phase cells. However, Celecoxib had the same effect by changing the G 1 phase cells and inducing apoptosis at higher concentration.
基金the grants from the National Outstanding Young Scien- tist Foundation of China (No. 39925035) the National Natural Science Foundations of China (No. 3967078 and No. 30070809)
文摘Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collected and expressions of Survivin and VEGF in peripheral blood were investigated by enzyme-linked immunosorbent assay （ELISA）. Results： Expressions of Survivin and VEGF were significantly associated with T stage, N stage and metastasis of squamous carcinoma of larynx. The patients with Survivin or VEGF over-expressions presented lower survival rate, respectively, as compared to those of low-expression （P 〈 0.05）. The survival rate in squamous carcinoma of larynx patients with Survivin and VEGF dual over-expressions was significantly lower than that of patients with dual low-expression （P 〈 0.05）. Multivariate analysis indicated that both Survivin and VEGF over-expressions in squamous carcinoma of larynx peripheral blood samples were strong independent factors of poor prognosis in squamous carcinoma of larynx patients. Conclusion： Survivin and VEGF over-expressions are independent prognostic factors for the patients with squamous carcinoma of larynx. These results also suggest that peripheral blood Survivin and VEGF expressions are valuable prognostic markers for prognosis prediction in squamous carcinoma of larynx patients.
基金supported by a grant from the Natural Science Foundation of Guangdong Province, P. R. China (No. 9151008901000223)
文摘Postoperative radiotherapy (PRT) is widely advocated for patients with squamous cell carcinomas of the head and neck that are considered to be at high risk of recurrence after surgical resection. The aims of this study were to evaluate the treatment outcomes of PRT for patients with laryngeal carcinoma and to identify the value of several prognostic factors. We reviewed the records of 256 patients treated for laryngeal squamous cell carcinoma between January 1993 and December 2005. Disease-free survival (DFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Log-rank test was employed to identify significant prognostic factors for DFS and OS. The Cox proportional hazards model was applied to identify covariates significantly associated with the aforementioned endpoints. Our results showed the 3-, 5-, and 10-year DFS for all patients were 69.9%, 59.5%, and 34.9%, respectively. The 3-, 5-, and 10-year OS rates were 80.8%, 68.6%, and 38.8%, respectively. Significant prognostic factors for both DFS and OS on univariate analysis were grade, primary site, T stage, N stage, overall stage, lymph node metastasis, overall treatment times of radiation, the interval between surgery and radiotherapy, and radiotherapy equipment. Favorable prognostic factors for both DFS and OS on multivariate analysis were lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment. In conclusion, our data suggest that lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment are favorable prognostic factors for DFS and OS and that reducing the overall treatment times of radiation to 6 weeks or less and the interval between surgery and radiotherapy to less than 3 weeks are simple measures to remarkably improve treatment outcome.
文摘Background: The current treatment options of early glottic carcinoma are radiotherapy;trans laser microsurgery, and open surgery. However, the best treatment is still controversial due to lack of randomized controlled trials. We aimed to evaluate the treatment results and the prognostic factors of local control of early glottic squamous cell carcinoma patients (GSCC) T1-2N0M0 treated at our institution. Material and Methods: We retrospectively studied the charts of 52 patients with early GSCC T1-T2N0M0 from 2010-2015 at the Clinical Oncology Department, Ain-Shams University. 24 patients had T1 and 28 had T2 early glottic carcinoma. The overall survival OS, local control rate, and laryngeal preservation rate were evaluated. Kaplan-Meier method, Cox proportional hazards model were used to analyze the data. Results: Median duration of follow-up was 13 months. Thirty-eight patients received radiation treatment alone (73.1%), 7.7% of the patients underwent surgery alone, and 19.2% of the patients had surgery combined with radiotherapy. Local recurrence after radiation failure developed in 6/52 patients, all had T2 disease and were salvaged by total laryngectomy. The ultimate local control rate was 88.5%, and the ultimate laryngeal preservation rate was 77.2% (40/52 patients). The median OS of the 52 patients was 13 months (range 2 - 46 months). Univariate analysis of factors associated with poor local control showed that age > 60 years was the only significant factor (P = 0.048). Conclusion: Radiotherapy achieves high local control and laryngeal preservation rates for patients with early glottic carcinoma, and is associated with a low rate of severe complications compared to surgery. Salvage surgery is feasible after radiotherapy failure.
文摘目的比较低温等离子射频消融术与CO2激光治疗早期声门型喉癌的临床疗效。方法选取2007年10月~2012年12月行手术治疗的104例早期声门型喉癌（Tis、T1a、T1b）患者,其中64例行低温等离子射频消融术（等离子组）,40例行CO2激光切除术（CO2激光组）。记录并比较两组手术时间、术后第二日疼痛程度[视觉模拟评分法（visual analog scale,VAS）]评分;所有患者于术后一周、1个月及3个月行电子喉镜及嗓音声学分析,并进行随访观察。结果等离子组平均手术时间为8.75±1.62min,CO2激光组平均手术时间为11.82±1.51min,等离子组优于CO2激光组（P〈0.05）。术后第二日VAS评分等离子组（2.79±0.47分）与CO2激光组（2.96±0.64分）组间差异无统计学意义（P〉0.05）。等离子组术后1个月粘膜恢复情况好于CO2激光组（χ2=13.842,P〈0.05）。单因素重复测量分析结果显示：等离子组及CO2激光组术后嗓音声学指标jitter、shimmer、HNR随术后时间延长逐渐好转,在术后3个时间点的变化差异有统计学意义（P〈0.05）,且呈线性变化趋势。多因素重复测量方差分析结果显示：嗓音声学指标（jitter、shimmer、HNR）在术后3个时间点两组间差异均有统计学意义（P〈0.05）。等离子组与CO2激光组术后3年生存率、局部复发率、侵及前联合复发率、前联合术后粘连率组间差异均无统计学意义（P〉0.05）。两组所有患者均未行气管切开术,术后无呛咳,无呼吸困难,无严重疼痛及咯血等严重并发症。结论低温等离子射频消融术与CO2激光治疗早期声门型喉癌均较安全、有效,而低温等离子射频消融术手术时间较CO2激光手术短,术后一月粘膜恢复及嗓音恢复均优于CO2激光手术。
