目的:探讨532nm激光不同光剂量参数及血卟啉单甲醚(HMME)不同浓度参数对体外培养的乳腺癌细胞(MCF-7)的光动力学杀伤作用。方法:MCF-7细胞用浓度分别为5μg/m l、10μg/m l、20μg/m l的HMME孵育2h后,以532nm半导体激光照射,能量密度分...目的:探讨532nm激光不同光剂量参数及血卟啉单甲醚(HMME)不同浓度参数对体外培养的乳腺癌细胞(MCF-7)的光动力学杀伤作用。方法:MCF-7细胞用浓度分别为5μg/m l、10μg/m l、20μg/m l的HMME孵育2h后,以532nm半导体激光照射,能量密度分别为0.3J/cm 2、0.6J/cm 2、1.2J/cm 2、2.4J/cm 2、4.8J/cm 2,24h后用CCK-8检测细胞存活率,并在光镜及电镜下观察细胞的形态变化。结果:随着激光剂量的增大,在一定的范围内,细胞的抑制率呈现上升趋势;能量密度为2.4J/cm 2,HM M E浓度为20μg/m l时,达到最大的抑制率为55.5%;并且细胞的抑制率也和H M M E浓度呈剂量依赖型;光镜下可观察到细胞坏死和凋亡样改变。结论:以532nm激光为光源的H M M E-PDT对M CF-7细胞有明显的杀伤作用,并在一定范围内呈光剂量和H M M E浓度剂量依赖型。展开更多
Bremachlorin®, also known as “Radachlorin®” and Photochlorin, a composition of 3 chlorophyll a derivatives in an aqueous solution [1], was initially introduced into the Russian and South Korean Pharmac...Bremachlorin®, also known as “Radachlorin®” and Photochlorin, a composition of 3 chlorophyll a derivatives in an aqueous solution [1], was initially introduced into the Russian and South Korean Pharmacopoeias with the corresponding marketing authorizations received in 2006 and 2008, on the basal cell skin carcinoma PDT protocol. Phase I-II clinical trials led in 2002-2005 showed no side effects, a good tolerability by patients and a high clinical anti-tumor efficacy. Further studies described in the present paper were fulfilled in accordance with a skin melanoma PDT + surgery combined technique protocol in 2006-2016. Resulting from the present trial, up to the 100% complete responses were achieved after resection and plastic surgery following PDT, with no melanoma regrowth or metastases during 5 and 10-year observation periods. Conducting PDT one day prior to tumor dissection can guarantee to meet the ablastics principles at the subsequent surgery. At the same time, the PDT + surgery protocol can allow for dissecting the tumor with less distance from its edge, which seems to be important for the face localizations of tumors.展开更多
文摘目的:探讨532nm激光不同光剂量参数及血卟啉单甲醚(HMME)不同浓度参数对体外培养的乳腺癌细胞(MCF-7)的光动力学杀伤作用。方法:MCF-7细胞用浓度分别为5μg/m l、10μg/m l、20μg/m l的HMME孵育2h后,以532nm半导体激光照射,能量密度分别为0.3J/cm 2、0.6J/cm 2、1.2J/cm 2、2.4J/cm 2、4.8J/cm 2,24h后用CCK-8检测细胞存活率,并在光镜及电镜下观察细胞的形态变化。结果:随着激光剂量的增大,在一定的范围内,细胞的抑制率呈现上升趋势;能量密度为2.4J/cm 2,HM M E浓度为20μg/m l时,达到最大的抑制率为55.5%;并且细胞的抑制率也和H M M E浓度呈剂量依赖型;光镜下可观察到细胞坏死和凋亡样改变。结论:以532nm激光为光源的H M M E-PDT对M CF-7细胞有明显的杀伤作用,并在一定范围内呈光剂量和H M M E浓度剂量依赖型。
文摘Bremachlorin®, also known as “Radachlorin®” and Photochlorin, a composition of 3 chlorophyll a derivatives in an aqueous solution [1], was initially introduced into the Russian and South Korean Pharmacopoeias with the corresponding marketing authorizations received in 2006 and 2008, on the basal cell skin carcinoma PDT protocol. Phase I-II clinical trials led in 2002-2005 showed no side effects, a good tolerability by patients and a high clinical anti-tumor efficacy. Further studies described in the present paper were fulfilled in accordance with a skin melanoma PDT + surgery combined technique protocol in 2006-2016. Resulting from the present trial, up to the 100% complete responses were achieved after resection and plastic surgery following PDT, with no melanoma regrowth or metastases during 5 and 10-year observation periods. Conducting PDT one day prior to tumor dissection can guarantee to meet the ablastics principles at the subsequent surgery. At the same time, the PDT + surgery protocol can allow for dissecting the tumor with less distance from its edge, which seems to be important for the face localizations of tumors.
