The effect of laser acupuncture on hypoxia tolerance and inflammation reaction in mice with optical fiber acupuncture needle intra body

Hypoxia is a disorder in which the tissues are not oxygenated adequately.The lack of oxygen may cause irreversible damage to the major organs such as the brain,heart and lungs.In severe cases,coma,seizures,and death may happen.In addition to the routine medication,acupuncture.treatment has been applied as a complementary treatment to counter hypoxia.In this paper,the optical fiber acupuncture needle was fabricated using the optical fiber imbedding into the metal capillary tube with needle tip,therefore made it easy to insert into the body for the laser treatment.The laser optical fiber needle treatment to the ST36 acupoint conducted with the laser.irradiation intra body.The normobaric hypoxia tolerance test results in mice show that the optical fber needle treatment to the ST36 acupoint with laser acupuncture appears to improve the tolerance to hypoxia.The mice treated with laser acupuncture expressed high level of IL-1βin serum.Our results suggest that laser optical fiber needle acupuncture may serve as a potential treatment for hypoxia.

E®ect of laser acupuncture combined with electrical stimulation on recovery from exercise fatigue in mice

In sports events,the rapid recovery after high-intensity training or sport competition performance is very important for athletes'performance and health.The aim of this study is to evaluate the effect of laser acupuncture and electrical stimulation on the recovery from exercise fatigue,using mice with swimming fatigue as experimental model and the electromyography(EMG)and the Raman spectroscopy of blood as evaluation indicators.Root mean square(RMS)and mean power frequency(MPF)of EMG were analyzed after laser acupuncture and electrical stimulation.The amplitude frequency combined analysis(JASA)showed that the proportion of muscles in the fatigue recovery area of the control group,the laser acupuncture group,the multi-channel laser acupuncture group and the laser combined with electrical stimulation group were 34.78%,39.13%,39.13%and 43.48%,respectively.Raman spectroscopy of the mice blood during fatigue recovery showed there is a significant difference between the multi-channel laser acupuncture group and the laser combined with electric stimulation group compared with the recovery period and fatigue period(P<0:05)at the peak of 997 cm^(-1) and the laser combined electrical stimulation group had a statistical difference in the recovery period compared with the fatigue period(P<0.05)at the peak of 1561 cm^(-1).The results showed that laser acupuncture combined with electrical stimulation was beneficial to fatigue recovery in mice,and had the potential value in sports fatigue recovery.

Thirty-two cases of type 2 diabetes mellitus of qi and yin deficiency treated with laser acupuncture

Objective To observe the clinical therapeutic effect of laser acupuncture on type 2 diabetes mellitus(T2DM)of qi and yin deficiency type.Methods Laser acupuncture was used to treat 32 patients of T2DM.Acupoint selection:Fèishū(肺俞BL13),Píshū(脾俞BL20),Shènshū(肾俞BL23),Wǎnxiàshū(胃脘下俞EX-B3),Sānyīnjiāo(三阴交SP6)and Zúsānlĭ足三里(ST36).It took 20 min in each treatment,five treatments a week.There were 2 days of interval in 2 weeks of treatment.Before and after treatment,the changes in fasting plasma glucose(FPG),postprandial 2 h glucose(2hPG),fasting insulin(FINS),glycosylated albumin(GA),insulin resistance index(HOMA-IR)and the symptom score of traditional Chinese medicine(TCM)were observed in the patients.The therapeutic effect was evaluated.Results Of 32 cases,8 cases were remarkably effective,18 cases effective and 6 cases failed.The total effective rate was 81.3%.At the end of treatment course,the level of every indicator after treatment was significantly lower than that before treatment(all P<0.01).Conclusion Laser acupuncture achieves the significant therapeutic effect on T2DM of qi and yin deficiency and this therapy deserves to be promoted in clinical application.

The Effect of Laser Acupuncture on the Levels of IL-2,IL-6 and TGF-β1in Serum of Rats with Atrophic Gastritis

Objective： To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 （IL-2）, inter- leukin 6 （IL-6） and transforming growth factor-β1 （TGF-β1） in rat＇s serum with chronic atrophic gastritis. Methods： 9 rats were randomly selected from 60 rats as normal con- trol group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination; when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chron- ically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points, after the treatment for 14 d, blood was removed, double antibody sandwich en- zyme linked immunosorbent assay （ELISA） was used to detect the levels of IL-2, IL-6 and TGF-β1 in serum. Results： Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased （P〈0.05 or P〈0.01）. Medicine, acupuncture and laser acupuncture could reduce the contents of IL-2, IL-6 and TGF-β1, IL-2 in serum of the medicine group and laser acupuncture group was signifi- cantly decreased （P〈0.05）; IL-6 in acupuncture group and laser acupuncture group were significantly decreased （P〈0.05 or P〈0.01）;and the most significant is laser acupuncture group （P〈0.01）. Medicine, acupuncture and laser acupuncture could significantly reduce TGF-β 1 content in serum （P〈0.05 or P〈0.01）, and the particularly significant was laser acupuncture（P〈0.01）. Conclusion： Laser acupuncture has the function of reducing the inflammatory response and adjusting immune function. It earl effectively reduce the ex- pression level of IL-2, IL-6 and TGF- β1 in serum of rats with chronic atrophic gastri- tis, and its effects are better than traditional acupuncture and medicine.

He-Ne laser acupuncture for 30 cases of unilateral nervous tinnitus

Objective： To observe the clinical effects of the unilateral nervous tinnitus treated by He-Ne laser acupuncture.Methods： A total of 30 patients of unilateral nervous tinnitus were treated with He-Ne laser acupuncture.The disposable filiform needles of 0.40 mm × 25 mm were used. Ermén（耳门 TE 21）, Tīnggōng（听宫 SI 19）,Tinghui（听会 GB 2）, Yìfēng（翳风 TE 17）,Shuàigǔ（率谷 GB 8） and Zhōngzhǔ（中渚 TE 3） were adopted on the affected side. The input and output ends of the fiber-optic catheter of the laser instrument were connected at a pair of acupoints（TE 21 connect to SI 19, GB 2 connect to TE 17, GB 8 connect to TE 3）.Each pair of acupoints was stimulated with He-Ne laser irradiation for 10 min, 8-10 mW in the output power and 6328 A in the wavelength. The needles were retained for 30 min totally. The treatment was given once a day, 5 times a week, continuously for 2 weeks.Results： Of 30 cases, 19 cases were cured clinically, accounting for 63.3%（19/30）; 7 cases effective remarkably, accounting for 23.3%（7/30） and 4 cases failed, accounting for 13.3%（4/30）.Conclusion： He-Ne laser acupuncture achieves the satisfactory effects on treating nervous tinnitus and it is easily accepted by the patients. Hence, this therapy deserves to be promoted in clinical application.

Alleviation of cartilage degeneration by laser acupuncture:An ultrasound biomicroscopic study of early postmenopausal osteoarthritis in rats

This study aims to evaluate the therapeutic effects of laser acupuncture(LA)on articular cartilage degradation at the early stage of postmenopausal osteoarthritis(PMOA)by an ultrasound biomicroscopic technology.Twentyone healthy female Sprague-Dawley rats were randomly and evenly divided into three groups-sham,ovariectomized(OVX)and OVXþLA groups.Only the peri-ovarian fatty tissue of the sham rats was exteriorized.The animals in the OVX and OVXþLA groups underwent bilateral ovariectomy to create a menopause model.On day 8 after the ovariectomy,the 2-week LA therapy at acupoints Guanyuan(CV-4),Sanyinjiao(SP-6),and Zusanli(ST36)was performed in the OVXþLA group,while the sham and OVX groups had a sham treatment.All the animals were sacrificed on day 22.The cartilage tissues in the left knee joint were examined by ultrasound and histology.Compared with the sham group,the significantly degenerate alterations(p<0.01)in ultrasound roughness index(URI),reflection coefficient(RC1)and cartilage thickness were found in the OVX group,while the values of these parameters were significantly recovered(p<0.01)in the OVXþLA group.The LA treatment enhanced proteoglycans(PGs)content with a significant reduce of the modified Mankin score(p<0.01).The acoustic assessment strongly correlated with the histologic evaluation.The results showed that LA treatment could alleviate the postmenopause-induced cartilage degradation and promote tissue repair.LA has potential to be a simple and safe non-pharmacological countermeasure against PMOA for geriatric patient population.The ultrasound biomicroscopic technology could be a quantitative evaluation tool of the structural and morphological responses of articular cartilage to LA intervention.

CLINICAL STUDY ON LASER NEEDLE-KNIFE AND ACUPUNCTURE FOR VERTEBRAL-ARTERY-TYPE CERVICAL SPONDYLOSIS

Objective To compare the effect of laser needle-knife and acupuncture with medication for vertebral-artery-type cervical spondylosis （CSA）. Methods The 88 outpatients of CSA were divided with single-blind method into Treatment Group （treated by laser needle-knife and acupuncture） with 48 patients in it and Control Group （treated by Intravenous drip of Compound Salvia Miltiorrhiza Injection and oral taking of Flunarizine （Sibelium） with 40 patients in it. Before the treatment, the two groups were without difference statistically. Results In the treatment group, the curative rate was 60.42% and the total effective rate 97.92%. In the control group, the curative rate was 30% and the total effective rate 87.5%. In statistics, P〈0.05, a significant difference existed between the two groups. Conclusion The effect of laser needleknife and acupuncture for CSA is obviously better than that of medication, worth spreading.

Laser therapy on points of acupuncture on nerve repair

Paresthesia is the name given to a temporary or permanent sensory loss caused by several surgical procedures that affected the peripheral sensory nerve.In dentistry,common iatrogenic procedures that can lead to sensory loss include third molar removal,

Application of Photobiomodulation Therapy in Acupuncture

Photobiomodulation(PBM)therapy is a therapeutic method that can produce a range of physiological effects in cells and tissues using certain wavelengths.The reparative benefits of PBM therapy include wound healing,bone regeneration,pain reduction,and the mitigation of inflammation.Advances in the development of laser instruments,including the use of high-intensity lasers in physiotherapy,have recently led to controllable photothermal and photomechanical treatments that enable therapeutic effects to be obtained without damaging tissue.The combination of PBM therapy with acupuncture may provide new perspectives for investigating the underlying therapeutic mechanisms of acupuncture and promote its widespread application.

Observation on Therapeutic Effects of Laser Radiation on Acupoints for Prostatic Hyperplasia

Objective： To observe the clinical effects in the treatment of prostatic hyperplasia by laser radiation on acupoints. Methods： Sixty cases were randomly divided into two groups, 30 cases in each group. 808 mm laser radiation on acupoints was used in the treatment group, and oral administration of Western medication, finasteride tablets, was used in the control group. After 3-course treatments, the clinical effects, international prostate syndrome scores （I-PSS）, quality of life index （QLI） and change of prostate volume were observed. Results：The total effective rate was 93.3% in the treatment group and 66.7% in the control group, with remarkable difference between the two groups （P0.05）. I-PSS, QLI and prostate volume were all improved in the two groups after treatments. In comparison of I-PSS and QLI after the treatments between the two groups, there were significant differences （P0.05）. Conclusion： The therapeutic effects of laser radiation on acupoints are precise in treating prostatic hyperplasia.

Treatment of Chronic Diarrhea with Point-irradiation of He-Ne Laser in 50 Cases

To treat 50 cases of chronic diarrhea by laser point-radiation on Shenque (CV 8), Tianshu (ST 25), Zusanli (ST 36) and Point Anti-diarrhea with low power He?Ne laser. The total effective rate was 96%. Key Words Laser Acupuncture - Diarrhea, Chronic - Acupuncture Therapy Author: WU Tong-fa (1936-), male, professor