Gene transfer introduced by physical methods is widely used for its convenient operation, high transfer efficiency and no host distinctive limitation. In this article pSV β Gal plasmid was induced into osteoblast cel...Gene transfer introduced by physical methods is widely used for its convenient operation, high transfer efficiency and no host distinctive limitation. In this article pSV β Gal plasmid was induced into osteoblast cells successfully by laser microbeam method and ultrasound method, and β Gal gene was highly expressed. The optimal conditions for the ultrasound and laser microbeam methods of gene transfer were obtained.展开更多
文摘Gene transfer introduced by physical methods is widely used for its convenient operation, high transfer efficiency and no host distinctive limitation. In this article pSV β Gal plasmid was induced into osteoblast cells successfully by laser microbeam method and ultrasound method, and β Gal gene was highly expressed. The optimal conditions for the ultrasound and laser microbeam methods of gene transfer were obtained.
