This article presents a review pertains the laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals. To improve artificial insemination, laser is sensitive and cost...This article presents a review pertains the laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals. To improve artificial insemination, laser is sensitive and cost effective technique, when compared to other conventional methods. Laser may have both positive and negative effects on spermatozoa functions. Since the effects of light are mediated by reactive oxygen species, and the levels of these reactive oxygen species following irradiating spermatozoa with laser may be responsible for determining the effects of laser on sperm. Dose of laser may be regarded as of great significance and this dosage of laser may be responsible for determining its effects on spermatozoa. Optimum dosage of laser for improving seminal attributes may vary among various species and this need to be standardized in each of them. The beneficial effects include improving sperm livability, acrosomal integrity, hypo-osmotic swelling response, mitochondrial function and computer-aided sperm analysis parameters. The increase in cytochrome c oxidase activity, ATP levels and mitochondrial membrane potential, in laser irradiated cells may be responsible for enhanced sperm quality parameters. Improving fertility with laser irradiated spermatozoa has been reported in few species like boar and need to be elaborated in other species. In conclusion laser may be regarded as an easy, cheap and time saving technology for improving artificial insemination;in addition, laser may have various potential applications in the field of reproductive biotechnology as well as in livestock farms and veterinary polyclinics.展开更多
Objective:To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and bucks.Methods:The levels of hydrogen peroxide(H2O2)production in ram and buck spermatozoa incubated...Objective:To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and bucks.Methods:The levels of hydrogen peroxide(H2O2)production in ram and buck spermatozoa incubated with 0(control),4 and 8 mM of pentoxifylline were measured after 45-min incubation.Then,the motility parameters of ram and buck spermatozoa incubated with 0(control),1 mM of H2O2,1 mM of H2O2 plus 4 mM of pentoxifylline,and 1 mM of H2O2 plus 8 mM of pentoxifylline were analysed.H2O2 was estimated using a fluorometric assay and spermatozoa motility characteristics were analyzed using computer aided sperm analyzer.Results:Pentoxifylline significantly decreased the levels of H2O2 produced from both ram and buck spermatozoa(P<0.05),and significant lower rates of H2O2 formation were noted when 8 mM of pentoxifylline was added(P<0.05).The values of all sperm motility parameters for the two species significantly decreased after incubation with H2O2(P<0.05).In contrast,when the spermatozoa were incubated with both H2O2 and two concentrations of pentoxifylline,the motility values rose significantly compared to those incubated with H2O2 alone(P<0.05).For both ram and buck sperm samples,the rapid and static subpopulation motility parameters were the most affected categories by pentoxifylline addition compared to the medium and slow categories.Conclusions:Pentoxifylline possesses an antioxidant capacity proved by its ability of reducing H2O2 levels as well as by increasing motility values of stressed spermatozoa.Therefore,pentoxifylline could be recommended as antioxidant additive for spermatozoa of small ruminants under stress conditions.展开更多
针对火炮光电窥膛系统中对激光靶图像自动识别和测量中的高效、准确、实时性的要求,设计了一套DSP(Digital Signal Processing)快速运算方法的硬件实施方案.介绍了基于高性能DSP实时处理的硬件原理框图,重点分析了硬件系统中的大量数据...针对火炮光电窥膛系统中对激光靶图像自动识别和测量中的高效、准确、实时性的要求,设计了一套DSP(Digital Signal Processing)快速运算方法的硬件实施方案.介绍了基于高性能DSP实时处理的硬件原理框图,重点分析了硬件系统中的大量数据交换瓶颈问题,存储器访问的软件优化和分配机制以及快速定标量化的压缩存储机制.实践证明该方案有效地提高了系统在实时测量中的应用价值.展开更多
文摘This article presents a review pertains the laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals. To improve artificial insemination, laser is sensitive and cost effective technique, when compared to other conventional methods. Laser may have both positive and negative effects on spermatozoa functions. Since the effects of light are mediated by reactive oxygen species, and the levels of these reactive oxygen species following irradiating spermatozoa with laser may be responsible for determining the effects of laser on sperm. Dose of laser may be regarded as of great significance and this dosage of laser may be responsible for determining its effects on spermatozoa. Optimum dosage of laser for improving seminal attributes may vary among various species and this need to be standardized in each of them. The beneficial effects include improving sperm livability, acrosomal integrity, hypo-osmotic swelling response, mitochondrial function and computer-aided sperm analysis parameters. The increase in cytochrome c oxidase activity, ATP levels and mitochondrial membrane potential, in laser irradiated cells may be responsible for enhanced sperm quality parameters. Improving fertility with laser irradiated spermatozoa has been reported in few species like boar and need to be elaborated in other species. In conclusion laser may be regarded as an easy, cheap and time saving technology for improving artificial insemination;in addition, laser may have various potential applications in the field of reproductive biotechnology as well as in livestock farms and veterinary polyclinics.
基金funded by Atomic Energy Commission,Syria(Project:36/ZM2-2021).
文摘Objective:To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and bucks.Methods:The levels of hydrogen peroxide(H2O2)production in ram and buck spermatozoa incubated with 0(control),4 and 8 mM of pentoxifylline were measured after 45-min incubation.Then,the motility parameters of ram and buck spermatozoa incubated with 0(control),1 mM of H2O2,1 mM of H2O2 plus 4 mM of pentoxifylline,and 1 mM of H2O2 plus 8 mM of pentoxifylline were analysed.H2O2 was estimated using a fluorometric assay and spermatozoa motility characteristics were analyzed using computer aided sperm analyzer.Results:Pentoxifylline significantly decreased the levels of H2O2 produced from both ram and buck spermatozoa(P<0.05),and significant lower rates of H2O2 formation were noted when 8 mM of pentoxifylline was added(P<0.05).The values of all sperm motility parameters for the two species significantly decreased after incubation with H2O2(P<0.05).In contrast,when the spermatozoa were incubated with both H2O2 and two concentrations of pentoxifylline,the motility values rose significantly compared to those incubated with H2O2 alone(P<0.05).For both ram and buck sperm samples,the rapid and static subpopulation motility parameters were the most affected categories by pentoxifylline addition compared to the medium and slow categories.Conclusions:Pentoxifylline possesses an antioxidant capacity proved by its ability of reducing H2O2 levels as well as by increasing motility values of stressed spermatozoa.Therefore,pentoxifylline could be recommended as antioxidant additive for spermatozoa of small ruminants under stress conditions.
文摘针对火炮光电窥膛系统中对激光靶图像自动识别和测量中的高效、准确、实时性的要求,设计了一套DSP(Digital Signal Processing)快速运算方法的硬件实施方案.介绍了基于高性能DSP实时处理的硬件原理框图,重点分析了硬件系统中的大量数据交换瓶颈问题,存储器访问的软件优化和分配机制以及快速定标量化的压缩存储机制.实践证明该方案有效地提高了系统在实时测量中的应用价值.
