Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as ...Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as a function of time in response to blocking and releasing of blood flow in a forearm are used as a measure of oxygen transport with blood to the tissue, which directly correlates with the skin microcirculation status. In this paper, a non-invasive dynamic monitoring system based on blood flow-mediated skin fluorescence (FMSF) technology is developed to monitor the NADH fluorescence intensity of skin tissue during the process of blocking reactive hyperemia. Simultaneously, laser speckle contrast imaging (LSCI) and laser Doppler flowmetry (LDF) were used to observe blood flow, blood oxygen saturation (SOt2) and relative amount of hemoglobin (rHb) during the measurement process, which helped to explore NADH dynamics relevant physiological changes. A variety of parameters have been derived to describe NADH fluorescence curve based on the FMSF device. The experimental results are conducive to understanding the NADH measurement and the physiological processes related to it, which help FMSF to be a great avenue for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.展开更多
目的:应用激光多普勒血流仪检测龋源性露髓成熟恒牙活髓保存术后的牙髓血流转归趋势。方法:收集2020年1月至2021年1月就诊于右江民族医学院附属医院牙体牙髓科的60例无症状因深龋或继发龋露髓的成熟恒牙,随机分为对照组和实验组,每组30...目的:应用激光多普勒血流仪检测龋源性露髓成熟恒牙活髓保存术后的牙髓血流转归趋势。方法:收集2020年1月至2021年1月就诊于右江民族医学院附属医院牙体牙髓科的60例无症状因深龋或继发龋露髓的成熟恒牙,随机分为对照组和实验组,每组30例,分别采用PMTA和iRoot BP Plus行活髓保存术处理,期间应用激光多普勒血流仪记录每组患牙术前与术后1、3、6、12个月的牙髓血流值,并对两组活髓保存术前和术后自身的血流值以及同一观察时间点两组间的血流值进行分析比较。结果:iRoot BP Plus组,术前血流值分别和术后1、3、6、12月之间的比较,以及术后3月与术后6月之间的比较,差异具有统计学意义(P<0.05),其余任意两组之间的比较,差异无统计学意义(P>0.05);MTA组,术前血流值分别和术后1、3、6、12月之间的比较,差异具有统计学意义(P<0.05),术后1、3、6、12月任意两组之间的比较,差异无统计学意义(P>0.05)。iRoot BP Plus组和MTA组在术后3月的血流值的比较差异具有统计学意义(P<0.05),iRoot BP Plus组较MTA组血流值稍高。结论:临床上对于因龋露髓的成熟恒牙,采用iRoot BP Plus或MTA行活髓保存术均可以获得较好的临床疗效;经过1年的随访观察,iRoot BP Plus组和MTA组的血流值能回归到正常临床参考值范围,其中MTA组的血流下降后维持较平稳。展开更多
Uroflowmetry is an uroflow measurement technique used to record the voided volume(VV)and flow rate,which can help clinicians objectively evaluate lower urinary tract function.Currently,few uroflowmetry studies have fo...Uroflowmetry is an uroflow measurement technique used to record the voided volume(VV)and flow rate,which can help clinicians objectively evaluate lower urinary tract function.Currently,few uroflowmetry studies have focused on the female population,[1]especially on the uroflow curve standard analysis.Hence,we sought to establish dependable uroflow reference values and nomograms for Chinese women and to evaluate the relationship between uroflow patterns and demographic data.展开更多
文摘Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as a function of time in response to blocking and releasing of blood flow in a forearm are used as a measure of oxygen transport with blood to the tissue, which directly correlates with the skin microcirculation status. In this paper, a non-invasive dynamic monitoring system based on blood flow-mediated skin fluorescence (FMSF) technology is developed to monitor the NADH fluorescence intensity of skin tissue during the process of blocking reactive hyperemia. Simultaneously, laser speckle contrast imaging (LSCI) and laser Doppler flowmetry (LDF) were used to observe blood flow, blood oxygen saturation (SOt2) and relative amount of hemoglobin (rHb) during the measurement process, which helped to explore NADH dynamics relevant physiological changes. A variety of parameters have been derived to describe NADH fluorescence curve based on the FMSF device. The experimental results are conducive to understanding the NADH measurement and the physiological processes related to it, which help FMSF to be a great avenue for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.
文摘目的:应用激光多普勒血流仪检测龋源性露髓成熟恒牙活髓保存术后的牙髓血流转归趋势。方法:收集2020年1月至2021年1月就诊于右江民族医学院附属医院牙体牙髓科的60例无症状因深龋或继发龋露髓的成熟恒牙,随机分为对照组和实验组,每组30例,分别采用PMTA和iRoot BP Plus行活髓保存术处理,期间应用激光多普勒血流仪记录每组患牙术前与术后1、3、6、12个月的牙髓血流值,并对两组活髓保存术前和术后自身的血流值以及同一观察时间点两组间的血流值进行分析比较。结果:iRoot BP Plus组,术前血流值分别和术后1、3、6、12月之间的比较,以及术后3月与术后6月之间的比较,差异具有统计学意义(P<0.05),其余任意两组之间的比较,差异无统计学意义(P>0.05);MTA组,术前血流值分别和术后1、3、6、12月之间的比较,差异具有统计学意义(P<0.05),术后1、3、6、12月任意两组之间的比较,差异无统计学意义(P>0.05)。iRoot BP Plus组和MTA组在术后3月的血流值的比较差异具有统计学意义(P<0.05),iRoot BP Plus组较MTA组血流值稍高。结论:临床上对于因龋露髓的成熟恒牙,采用iRoot BP Plus或MTA行活髓保存术均可以获得较好的临床疗效;经过1年的随访观察,iRoot BP Plus组和MTA组的血流值能回归到正常临床参考值范围,其中MTA组的血流下降后维持较平稳。
文摘Uroflowmetry is an uroflow measurement technique used to record the voided volume(VV)and flow rate,which can help clinicians objectively evaluate lower urinary tract function.Currently,few uroflowmetry studies have focused on the female population,[1]especially on the uroflow curve standard analysis.Hence,we sought to establish dependable uroflow reference values and nomograms for Chinese women and to evaluate the relationship between uroflow patterns and demographic data.
