Traditional laparoscopic liver cancer resection faces challenges,such as difficultiesin tumor localization and accurate marking of liver segments,as well as theinability to provide real-time intraoperative navigation....Traditional laparoscopic liver cancer resection faces challenges,such as difficultiesin tumor localization and accurate marking of liver segments,as well as theinability to provide real-time intraoperative navigation.This approach falls shortof meeting the demands for precise and anatomical liver resection.The introductionof fluorescence imaging technology,particularly indocyanine green,hasdemonstrated significant advantages in visualizing bile ducts,tumor localization,segment staining,microscopic lesion display,margin examination,and lymphnode visualization.This technology addresses the inherent limitations oftraditional laparoscopy,which lacks direct tactile feedback,and is increasinglybecoming the standard in laparoscopic procedures.Guided by fluorescenceimaging technology,laparoscopic liver cancer resection is poised to become thepredominant technique for liver tumor removal,enhancing the accuracy,safetyand efficiency of the procedure.展开更多
Development of innovative nanomedicine enabling enhanced theranostics of multidrug-resistant(MDR)tumors remains to be challenging.Herein,we report the development of a newly designed multifunctional yellowfluorescent ...Development of innovative nanomedicine enabling enhanced theranostics of multidrug-resistant(MDR)tumors remains to be challenging.Herein,we report the development of a newly designed multifunctional yellowfluorescent carbon dot(y-CD)/dendrimer nanohybrids as a platform for ultrasound(US)-enhanced fluorescence imaging and chemotherapy of MDR tumors.Generation 5(G5)poly(amidoamine)dendrimers covalently modified with efflux inhibitor of D-α-tocopheryl polyethylene glycol 1000 succinate(G5-TPGS)were complexed with one-step hydrothermally synthesized y-CDs via electrostatic interaction.The formed G5-TPGS@y-CDs complexes were then physically loaded with anticancer drug doxorubicin(DOX)to generate(G5-TPGS@y-CDs)-DOX complexes.The developed nanohybrids display a high drug loading efficiency(40.7%),strong y-CD-induced fluorescence emission,and tumor microenvironment pH-preferred DOX release profile.Attributing to the DOX/TPGS dual drug design,the(G5-TPGS@y-CDs)-DOX complexes can overcome the multidrug resistance(MDR)of cancer cells and effectively inhibit the growth of cancer cells and tumors.Furthermore,the introduction of US-targeted microbubble destruction technology was proven to render the complexes with enhanced intracellular uptake and anticancer efficacy in vitro and improved chemotherapeutic efficacy and fluorescence imaging of tumors in vivo due to the produced sonoporation effect.The developed multifunctional dendrimer/CD nanohybrids may represent an advanced design of nanomedicine for US-enhanced theranostics of different types of MDR tumors.展开更多
随着吲哚菁绿荧光显像技术(indocyanine green fluorescent imaging,ICG-FI)越发成熟,加之多种染色及显像方式,经济快捷的突出优点,广泛应用于肝细胞癌解剖性切除,精准作用在肿瘤及边界定位,更好发现微小病灶;用于手术肝切平面选择和监...随着吲哚菁绿荧光显像技术(indocyanine green fluorescent imaging,ICG-FI)越发成熟,加之多种染色及显像方式,经济快捷的突出优点,广泛应用于肝细胞癌解剖性切除,精准作用在肿瘤及边界定位,更好发现微小病灶;用于手术肝切平面选择和监测,大大增加残留癌灶的检出概率,有效减低术后复发转移,最大限度实现R0切除并显著降低了手术难度,为腹腔镜下精准肝切除开辟全新的道路。但吲哚菁绿(indocyanine green,ICG)也存在假阳性结果,易受肝脏功能、给药时间和剂量、显影剂不良反应等局限因素影响,其效能及可靠性仍需探索研究。总之由于腹腔镜肝脏肿瘤切除手术过程精细、复杂又耗时长,且患者个体差异性较大,在临床中还需根据患者实际病情进行个体化诊疗。笔者现就吲哚菁绿荧光显像技术在腹腔镜下肝细胞癌切除术中应用的研究进展进行综述。展开更多
文摘Traditional laparoscopic liver cancer resection faces challenges,such as difficultiesin tumor localization and accurate marking of liver segments,as well as theinability to provide real-time intraoperative navigation.This approach falls shortof meeting the demands for precise and anatomical liver resection.The introductionof fluorescence imaging technology,particularly indocyanine green,hasdemonstrated significant advantages in visualizing bile ducts,tumor localization,segment staining,microscopic lesion display,margin examination,and lymphnode visualization.This technology addresses the inherent limitations oftraditional laparoscopy,which lacks direct tactile feedback,and is increasinglybecoming the standard in laparoscopic procedures.Guided by fluorescenceimaging technology,laparoscopic liver cancer resection is poised to become thepredominant technique for liver tumor removal,enhancing the accuracy,safetyand efficiency of the procedure.
基金supported by the Science and Technology Commission of Shanghai Municipality(20520710300,19XD1400100,19YF1440400, 19410740200)the National Key R&D Program(2017YFE0196200)the National Natural Science Foundation of China(81761148028 , 21773026).
文摘Development of innovative nanomedicine enabling enhanced theranostics of multidrug-resistant(MDR)tumors remains to be challenging.Herein,we report the development of a newly designed multifunctional yellowfluorescent carbon dot(y-CD)/dendrimer nanohybrids as a platform for ultrasound(US)-enhanced fluorescence imaging and chemotherapy of MDR tumors.Generation 5(G5)poly(amidoamine)dendrimers covalently modified with efflux inhibitor of D-α-tocopheryl polyethylene glycol 1000 succinate(G5-TPGS)were complexed with one-step hydrothermally synthesized y-CDs via electrostatic interaction.The formed G5-TPGS@y-CDs complexes were then physically loaded with anticancer drug doxorubicin(DOX)to generate(G5-TPGS@y-CDs)-DOX complexes.The developed nanohybrids display a high drug loading efficiency(40.7%),strong y-CD-induced fluorescence emission,and tumor microenvironment pH-preferred DOX release profile.Attributing to the DOX/TPGS dual drug design,the(G5-TPGS@y-CDs)-DOX complexes can overcome the multidrug resistance(MDR)of cancer cells and effectively inhibit the growth of cancer cells and tumors.Furthermore,the introduction of US-targeted microbubble destruction technology was proven to render the complexes with enhanced intracellular uptake and anticancer efficacy in vitro and improved chemotherapeutic efficacy and fluorescence imaging of tumors in vivo due to the produced sonoporation effect.The developed multifunctional dendrimer/CD nanohybrids may represent an advanced design of nanomedicine for US-enhanced theranostics of different types of MDR tumors.
文摘随着吲哚菁绿荧光显像技术(indocyanine green fluorescent imaging,ICG-FI)越发成熟,加之多种染色及显像方式,经济快捷的突出优点,广泛应用于肝细胞癌解剖性切除,精准作用在肿瘤及边界定位,更好发现微小病灶;用于手术肝切平面选择和监测,大大增加残留癌灶的检出概率,有效减低术后复发转移,最大限度实现R0切除并显著降低了手术难度,为腹腔镜下精准肝切除开辟全新的道路。但吲哚菁绿(indocyanine green,ICG)也存在假阳性结果,易受肝脏功能、给药时间和剂量、显影剂不良反应等局限因素影响,其效能及可靠性仍需探索研究。总之由于腹腔镜肝脏肿瘤切除手术过程精细、复杂又耗时长,且患者个体差异性较大,在临床中还需根据患者实际病情进行个体化诊疗。笔者现就吲哚菁绿荧光显像技术在腹腔镜下肝细胞癌切除术中应用的研究进展进行综述。
