Objective To establish allergic airway inflammation model in late-phase airway reaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided into three groups-, control group (Group Ⅰ) ,singl...Objective To establish allergic airway inflammation model in late-phase airway reaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided into three groups-, control group (Group Ⅰ) ,single challenge group (Group Ⅱ ),consecutive challenge group (Group Ⅲ). The rats in Group Ⅱ and Group Ⅲ were sensitized twice by injection of ovalbumin (OA) together with aluminum hydroxide and Bordetella pertussis as adjuvants, followed by challenge with aerosolized OA for 20 min once in Group Ⅱ or one time on each day for one week in, Group Ⅲ. The rats in Group Ⅰ received 0. 9% saline by injection and inhalation. Results Compared with group Ⅰ , there were positive symptoms observed in the group Ⅱ and group Ⅲ; the amount of total leucocytes and eosinophil percentage in brochoalveolar lavage fluid (BALF) significantly increased (P<0.05 or P<0. 01 respectively) in Group Ⅱ or Ⅲ ; histopathologic changes of lung showed acute allergic inflammation changes in Group Ⅱ: Disrupted epithelium damaged subepithelial structure and eosinophil infiltration in the airway wall. As for the Group Ⅲ, there were allergen- induced characteristic features of chronic allergic airways inflammation: hypertrophy and hyperplasia of bronchial smooth muscle, goblet cell hyperplasia, basement membrane thickening, eosinophil in filtration, edema. Conclusion The model of allergic airway inflammation in late-phase response of SD rats was successfully established by OA sensitization (twice) and consecutive challenge.展开更多
The apatite fission track dating of samples from the Dabashan （i.e., the Langshan in the northeastern Alxa Block） by the laser ablation method and their thermal history modeling of AFT ages are conducted in this stu...The apatite fission track dating of samples from the Dabashan （i.e., the Langshan in the northeastern Alxa Block） by the laser ablation method and their thermal history modeling of AFT ages are conducted in this study. The obtained results and lines of geological evidence in the study region indicate that the Langshan has experienced complicated tectonic-thermal events during the the Late Cretaceous-Cenozoic. Firstly, it experienced a tectonic-thermal event in the Late Cretaceous （-90-70 Ma）. The event had little relation with the oblique subduction of the Izanagi Plate along the eastern Eurasian Plate, but was related to the Neo-Tethys subduction and compression between the Lhasa Block and Qiangtang Block. Secondly, it underwent the dextral slip faulting in the Eocene （-50-45 Ma）. The strike slip fault may develop in the same tectonic setting as sinistral slip faults in southern Mongolia and thrusts in West Qinling to the southwest Ordos Block in the same period, which is the remote far-field response to the India-Eurasia collision. Thirdly, the tectonic thermal event existed in the late Cenozoic （since -10 Ma）, thermal modeling shows that several samples began their denudation from upper region of partial annealing zone （PAZ）, and the denudation may have a great relationship with the growth of Qinghai-Tibetan Plateau to the northeast. In addition, the AFT ages of Langshan indicate that the main body of the Langshan may be an upper part of fossil PAZ of the Late Cretaceous （-70 Ma）. The fossil PAZ were destroyed and deformed by tectonic events repeatedly in the Cenozoic along with the denudation.展开更多
基金Supported by the Natural Science Foundation of Jiangsu Province(BK2001160)
文摘Objective To establish allergic airway inflammation model in late-phase airway reaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided into three groups-, control group (Group Ⅰ) ,single challenge group (Group Ⅱ ),consecutive challenge group (Group Ⅲ). The rats in Group Ⅱ and Group Ⅲ were sensitized twice by injection of ovalbumin (OA) together with aluminum hydroxide and Bordetella pertussis as adjuvants, followed by challenge with aerosolized OA for 20 min once in Group Ⅱ or one time on each day for one week in, Group Ⅲ. The rats in Group Ⅰ received 0. 9% saline by injection and inhalation. Results Compared with group Ⅰ , there were positive symptoms observed in the group Ⅱ and group Ⅲ; the amount of total leucocytes and eosinophil percentage in brochoalveolar lavage fluid (BALF) significantly increased (P<0.05 or P<0. 01 respectively) in Group Ⅱ or Ⅲ ; histopathologic changes of lung showed acute allergic inflammation changes in Group Ⅱ: Disrupted epithelium damaged subepithelial structure and eosinophil infiltration in the airway wall. As for the Group Ⅲ, there were allergen- induced characteristic features of chronic allergic airways inflammation: hypertrophy and hyperplasia of bronchial smooth muscle, goblet cell hyperplasia, basement membrane thickening, eosinophil in filtration, edema. Conclusion The model of allergic airway inflammation in late-phase response of SD rats was successfully established by OA sensitization (twice) and consecutive challenge.
基金funded by Natural Science Foundation of China(41572190)National Basic Research Program of Ministry of Science and Technology of the People's Republic of China(2015CB453002)China Geological Survey(121201102000150009-16,12120115069601)
文摘The apatite fission track dating of samples from the Dabashan （i.e., the Langshan in the northeastern Alxa Block） by the laser ablation method and their thermal history modeling of AFT ages are conducted in this study. The obtained results and lines of geological evidence in the study region indicate that the Langshan has experienced complicated tectonic-thermal events during the the Late Cretaceous-Cenozoic. Firstly, it experienced a tectonic-thermal event in the Late Cretaceous （-90-70 Ma）. The event had little relation with the oblique subduction of the Izanagi Plate along the eastern Eurasian Plate, but was related to the Neo-Tethys subduction and compression between the Lhasa Block and Qiangtang Block. Secondly, it underwent the dextral slip faulting in the Eocene （-50-45 Ma）. The strike slip fault may develop in the same tectonic setting as sinistral slip faults in southern Mongolia and thrusts in West Qinling to the southwest Ordos Block in the same period, which is the remote far-field response to the India-Eurasia collision. Thirdly, the tectonic thermal event existed in the late Cenozoic （since -10 Ma）, thermal modeling shows that several samples began their denudation from upper region of partial annealing zone （PAZ）, and the denudation may have a great relationship with the growth of Qinghai-Tibetan Plateau to the northeast. In addition, the AFT ages of Langshan indicate that the main body of the Langshan may be an upper part of fossil PAZ of the Late Cretaceous （-70 Ma）. The fossil PAZ were destroyed and deformed by tectonic events repeatedly in the Cenozoic along with the denudation.
