Acanthopagrus latus is an essential aquaculture species on the south coast of China.However,there is a lack of systematic breeding of A.latus,which considerably limits the sustainable development of A.latus.As a resul...Acanthopagrus latus is an essential aquaculture species on the south coast of China.However,there is a lack of systematic breeding of A.latus,which considerably limits the sustainable development of A.latus.As a result,genetic improvements are urgently needed to breed new strains of A.latus with rapid growth and strong resistance to disease.During selective breeding,it is necessary to estimate the genetic parameters of the target trait,which in turn depends on an accurate disentangled pedigree for the selective population.Therefore,it is necessary to establish the parentage assignment technique for A.latus.In this study,95 individuals selected from their parents and their 14 families were used as experimental material.SNPs were developed by genome resequencing,and highly polymorphic SNPs were screened on the basis of optimized filtering parameters.A total of 14392738 SNPs were discovered and 205 SNPs were selected for parentage assignment using the CERVUS software.In the model where the gender of the parents is known,the assignment success rate is 98.61%for the male parent,97.22%for the female parent,and 95.83%for the parent pair.In the model where the gender of the parents is unknown,the assignment success rate is 100%for a single parent and 90.28%for the parent pair.The results of this study were expected to serve as a reference for the breeding of new varieties of A.latus.展开更多
In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·...In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·L^-1 （sucrose 36.6g·L^-1 , fructose 26.0g·L^-1 , glucose 21.8g·L^-1 and other sugars 21.1g·L^-1 ）. Each inoculums （ 10%, v/v） was separately cultivated in the medium containing 20g·L^-1 total sugars under condition （30℃, 200 rpm, pH 6.5-7）. It was found that the A. eutrophus can be grown better than the A. latus. Only the A. eutrophus was further cultured under different total sugar concentrations （20, 30, 40 and 50g·L^-1 ）. The optimal contents of total sugar, dry cell mass （DCM） and maximum PHAs were obtained at 50g·L^-1 , 6.013g·L^-1 and 1.84g·L^-1 , respectively after 60 h fermentation which were converted to biomass yield （Yx/s）, product yield （Yp/5）, specific product yield （Yp/x） and productivity of 0.163, 0.05, 0.306 and 0.031 g.Llhl. Large scale of PHAs production was conducted in 5 L fermentor using the optimal condition obtained under 30% dissolved oxygen. The DCM and the maximum PHAs were 5.881g·L^-1 and 1.281g·L^-1 which were calculated to values of Yx/s, Yp/s, Yp/x and productivity at 0.19, 0.04, 0.218 and 0.021g·L^-1 , respectively.展开更多
Yellowfin seabream Acanthopagrus latus is an important economic fish in Chinese coastal areas.Given its narrow distribution and overfishing,the genetic diversity of yellowfin seabream has been restricted for artificia...Yellowfin seabream Acanthopagrus latus is an important economic fish in Chinese coastal areas.Given its narrow distribution and overfishing,the genetic diversity of yellowfin seabream has been restricted for artificial breeding and reproduction.We performed full-length transcriptome sequencing and assembly of the genome of yellowfin seabream.A total of 68086 unigenes were obtained,with an N50 of 3391 bp on average length of 2933 bp.A total number of 50593 expressed sequence tags linked to simple sequence repeats(EST-SSR)were identified,among them dinucleotide repeats(40.6%)and AC/GT motifs(38.5%)were the most frequent.Of the 190 EST-SSRs for which PCR primer pairs were designed,150 primer pairs successfully amplified target loci and 15 SSRs showed high polymorphism.The alleles per locus ranged 6-50 on average of 25.3.The expected and observed heterozygosity varied from 0.632 to 0.969 and from 0.519 to 0.953,respectively.The polymorphic index content(PIC)values of each locus ranged 0.587-0.966 on average of 0.851.Among six yellowfin seabream population samples preliminarily tested for genetic diversity and differentiation,the Fangchenggang(FCG)population in Guangxi Province had the highest mean observed heterozygosity(H_(o))value(0.786),whereas the Zhangzhou(ZZ)population in Fujian Province had the lowest(0.678).The pairwise fixation index(Fst)values indicated significant population differentiation among six yellowfin seabream populations.This study provided evidence for the usefulness of the transcriptomic resource information and EST-SSR markers for natural resource conservation,population genetics,and breeding studies of yellowfin seabream in South China.展开更多
Serum biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of water...Serum biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of waters and related soils. The aim of this research was to determine the reference values of biochemical factors in Acantopagrus latus and Epinephelus coioides. Blood samples were collected from captured fish from coastal waters of Chabahar (Ramin waterfront). Serum levels of glucose, Blood Urea Nitrogen (BUN), cholesterol (CHO), triglyceride, total protein, albumin, calcium (Ca2+), phosphorus (P), sodium (Na+), bilirubin and potassium (K+) were measured. The results showed that the levels of albumin and bilirubin in E. coioides were significantly higher than A. latus and there were significant differences in all of the serum values (except P and glucose) between A. latus and E. coioides (P < 0.05). It can be concluded that the values of blood biochemical parameters may be affected by physiological factors such as the species of fish.展开更多
采用同源克隆和RACE-PCR(Rap id amp lification of DNA ends-PCR)技术,从黄鳍鲷Acanthopgrus latus中克隆了白细胞介素1β(interleuk in-1,βIL-1β)基因的全长cDNA序列。黄鳍鲷IL-1β的cDNA全长共1 242 bp,5端非编码区(UTR)为121 bp,...采用同源克隆和RACE-PCR(Rap id amp lification of DNA ends-PCR)技术,从黄鳍鲷Acanthopgrus latus中克隆了白细胞介素1β(interleuk in-1,βIL-1β)基因的全长cDNA序列。黄鳍鲷IL-1β的cDNA全长共1 242 bp,5端非编码区(UTR)为121 bp,3端非编码区(UTR)为342 bp,开放阅读框(open read ing fram e,ORF)为762 bp,编码一条253个氨基酸残基的多肽,分子量约为28.5kDa,理论等电点为5.55,含有2个潜在的糖基化位点。同源性分析表明与其它鱼类和脊椎动物的IL-1β序列具有较高的同源性。利用软件S ignalP分析表明它不存在信号肽序列,氨基酸序列中不存在IL-1β转换酶(interleuk in-1βconverting enzym e,ICE)剪切位点,推导可能成熟肽为从第85位氨基酸开始共169个氨基酸残基的多肽,分子量约为18.9kDa。将此成熟肽序列克隆入pQE30质粒构建表达载体pQE30-IL1β,并在大肠杆菌Escherichia coliM15(pREP4)中进行诱导表达,获得了分子量约为21 kDa的特异性融合蛋白。展开更多
ZHULING POLYSACCHARIDE IN-JECTION(ZPI)is extracted from theChinese materia medica,Polyporus Umbel-latus.Compatible application of this medicain the treatment of malignant tumors andhepatitis B was recently reported in...ZHULING POLYSACCHARIDE IN-JECTION(ZPI)is extracted from theChinese materia medica,Polyporus Umbel-latus.Compatible application of this medicain the treatment of malignant tumors andhepatitis B was recently reported in the litera-ture.In our hospital,ZPI was used in treat-ing debilitated children with hypoimmunity.The results are reported in the following.展开更多
941547 紫红笛鲷(Lufjomus argentimacu-latus)的生物学和养殖=Biology and cultureof the red snapper,Lutjanus argentimacu-latus[告,英]/Doi M,Singhagraiwan T.—The research project of fishery resource de-volopment in the Ki...941547 紫红笛鲷(Lufjomus argentimacu-latus)的生物学和养殖=Biology and cultureof the red snapper,Lutjanus argentimacu-latus[告,英]/Doi M,Singhagraiwan T.—The research project of fishery resource de-volopment in the Kingdom of Thailand:TheEastern Marine Fisheries Devolopment Cen-ter.—1993.—51这是泰国东部海洋渔业发展中心(EMDEC)展开更多
Copolyesters of 3 hydroxybutyrate and 4 hydroxybutyrate along with terpolyesters of 3 hydroxybutyrate, 3 hydroxyvalerate and 4 hydroxybutyrate were produced using Alcaligenes latus DSM 1124 in growth media with p...Copolyesters of 3 hydroxybutyrate and 4 hydroxybutyrate along with terpolyesters of 3 hydroxybutyrate, 3 hydroxyvalerate and 4 hydroxybutyrate were produced using Alcaligenes latus DSM 1124 in growth media with precursors of 1,4 butanediol or 1,4 butanediol and valerate. Monomer mass fraction w in the copolyesters varied from 63% to 78% 3 hydroxybutyrate and 1 5% to 6 5% 4 hydroxybutyrate (cell dry weight), depending on precursor supplied. w(monomer) in the terpolyesters varied from 55% to 69% 3 hydroxybutyrate, 0 5% to 3 4% 4 hydroxybutyrate and 3 4% to 16% 3 hydroxyvalerate.展开更多
Almost seven decades have elapsed since the establishment of the Chouniugou Formation. This formation used to be considered as a mark for the recognition, division and correlation of the Late Visean biostratigraphic u...Almost seven decades have elapsed since the establishment of the Chouniugou Formation. This formation used to be considered as a mark for the recognition, division and correlation of the Late Visean biostratigraphic unit in China due to its abundant marine biotas such as gigantoproductids and coral Yuanophyllum. Although many geologists and palaeontologists had reinvestigated the typical section of this formation, they came to展开更多
基金Fujian Province science and technology plan project under contract No.2023N0011。
文摘Acanthopagrus latus is an essential aquaculture species on the south coast of China.However,there is a lack of systematic breeding of A.latus,which considerably limits the sustainable development of A.latus.As a result,genetic improvements are urgently needed to breed new strains of A.latus with rapid growth and strong resistance to disease.During selective breeding,it is necessary to estimate the genetic parameters of the target trait,which in turn depends on an accurate disentangled pedigree for the selective population.Therefore,it is necessary to establish the parentage assignment technique for A.latus.In this study,95 individuals selected from their parents and their 14 families were used as experimental material.SNPs were developed by genome resequencing,and highly polymorphic SNPs were screened on the basis of optimized filtering parameters.A total of 14392738 SNPs were discovered and 205 SNPs were selected for parentage assignment using the CERVUS software.In the model where the gender of the parents is known,the assignment success rate is 98.61%for the male parent,97.22%for the female parent,and 95.83%for the parent pair.In the model where the gender of the parents is unknown,the assignment success rate is 100%for a single parent and 90.28%for the parent pair.The results of this study were expected to serve as a reference for the breeding of new varieties of A.latus.
文摘In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·L^-1 （sucrose 36.6g·L^-1 , fructose 26.0g·L^-1 , glucose 21.8g·L^-1 and other sugars 21.1g·L^-1 ）. Each inoculums （ 10%, v/v） was separately cultivated in the medium containing 20g·L^-1 total sugars under condition （30℃, 200 rpm, pH 6.5-7）. It was found that the A. eutrophus can be grown better than the A. latus. Only the A. eutrophus was further cultured under different total sugar concentrations （20, 30, 40 and 50g·L^-1 ）. The optimal contents of total sugar, dry cell mass （DCM） and maximum PHAs were obtained at 50g·L^-1 , 6.013g·L^-1 and 1.84g·L^-1 , respectively after 60 h fermentation which were converted to biomass yield （Yx/s）, product yield （Yp/5）, specific product yield （Yp/x） and productivity of 0.163, 0.05, 0.306 and 0.031 g.Llhl. Large scale of PHAs production was conducted in 5 L fermentor using the optimal condition obtained under 30% dissolved oxygen. The DCM and the maximum PHAs were 5.881g·L^-1 and 1.281g·L^-1 which were calculated to values of Yx/s, Yp/s, Yp/x and productivity at 0.19, 0.04, 0.218 and 0.021g·L^-1 , respectively.
基金Supported by the National Key R&D Program of China (No. 2019YFD0901202)the Key-Area Research and Development Program of Guangdong Province (No. 2021B0202020002)+1 种基金the China Postdoctoral Science Foundation (No. 2021M693677)the Yellow Fin Bream Seed System Building Project (2021)
文摘Yellowfin seabream Acanthopagrus latus is an important economic fish in Chinese coastal areas.Given its narrow distribution and overfishing,the genetic diversity of yellowfin seabream has been restricted for artificial breeding and reproduction.We performed full-length transcriptome sequencing and assembly of the genome of yellowfin seabream.A total of 68086 unigenes were obtained,with an N50 of 3391 bp on average length of 2933 bp.A total number of 50593 expressed sequence tags linked to simple sequence repeats(EST-SSR)were identified,among them dinucleotide repeats(40.6%)and AC/GT motifs(38.5%)were the most frequent.Of the 190 EST-SSRs for which PCR primer pairs were designed,150 primer pairs successfully amplified target loci and 15 SSRs showed high polymorphism.The alleles per locus ranged 6-50 on average of 25.3.The expected and observed heterozygosity varied from 0.632 to 0.969 and from 0.519 to 0.953,respectively.The polymorphic index content(PIC)values of each locus ranged 0.587-0.966 on average of 0.851.Among six yellowfin seabream population samples preliminarily tested for genetic diversity and differentiation,the Fangchenggang(FCG)population in Guangxi Province had the highest mean observed heterozygosity(H_(o))value(0.786),whereas the Zhangzhou(ZZ)population in Fujian Province had the lowest(0.678).The pairwise fixation index(Fst)values indicated significant population differentiation among six yellowfin seabream populations.This study provided evidence for the usefulness of the transcriptomic resource information and EST-SSR markers for natural resource conservation,population genetics,and breeding studies of yellowfin seabream in South China.
文摘Serum biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of waters and related soils. The aim of this research was to determine the reference values of biochemical factors in Acantopagrus latus and Epinephelus coioides. Blood samples were collected from captured fish from coastal waters of Chabahar (Ramin waterfront). Serum levels of glucose, Blood Urea Nitrogen (BUN), cholesterol (CHO), triglyceride, total protein, albumin, calcium (Ca2+), phosphorus (P), sodium (Na+), bilirubin and potassium (K+) were measured. The results showed that the levels of albumin and bilirubin in E. coioides were significantly higher than A. latus and there were significant differences in all of the serum values (except P and glucose) between A. latus and E. coioides (P < 0.05). It can be concluded that the values of blood biochemical parameters may be affected by physiological factors such as the species of fish.
文摘采用同源克隆和RACE-PCR(Rap id amp lification of DNA ends-PCR)技术,从黄鳍鲷Acanthopgrus latus中克隆了白细胞介素1β(interleuk in-1,βIL-1β)基因的全长cDNA序列。黄鳍鲷IL-1β的cDNA全长共1 242 bp,5端非编码区(UTR)为121 bp,3端非编码区(UTR)为342 bp,开放阅读框(open read ing fram e,ORF)为762 bp,编码一条253个氨基酸残基的多肽,分子量约为28.5kDa,理论等电点为5.55,含有2个潜在的糖基化位点。同源性分析表明与其它鱼类和脊椎动物的IL-1β序列具有较高的同源性。利用软件S ignalP分析表明它不存在信号肽序列,氨基酸序列中不存在IL-1β转换酶(interleuk in-1βconverting enzym e,ICE)剪切位点,推导可能成熟肽为从第85位氨基酸开始共169个氨基酸残基的多肽,分子量约为18.9kDa。将此成熟肽序列克隆入pQE30质粒构建表达载体pQE30-IL1β,并在大肠杆菌Escherichia coliM15(pREP4)中进行诱导表达,获得了分子量约为21 kDa的特异性融合蛋白。
文摘ZHULING POLYSACCHARIDE IN-JECTION(ZPI)is extracted from theChinese materia medica,Polyporus Umbel-latus.Compatible application of this medicain the treatment of malignant tumors andhepatitis B was recently reported in the litera-ture.In our hospital,ZPI was used in treat-ing debilitated children with hypoimmunity.The results are reported in the following.
文摘941547 紫红笛鲷(Lufjomus argentimacu-latus)的生物学和养殖=Biology and cultureof the red snapper,Lutjanus argentimacu-latus[告,英]/Doi M,Singhagraiwan T.—The research project of fishery resource de-volopment in the Kingdom of Thailand:TheEastern Marine Fisheries Devolopment Cen-ter.—1993.—51这是泰国东部海洋渔业发展中心(EMDEC)
文摘Copolyesters of 3 hydroxybutyrate and 4 hydroxybutyrate along with terpolyesters of 3 hydroxybutyrate, 3 hydroxyvalerate and 4 hydroxybutyrate were produced using Alcaligenes latus DSM 1124 in growth media with precursors of 1,4 butanediol or 1,4 butanediol and valerate. Monomer mass fraction w in the copolyesters varied from 63% to 78% 3 hydroxybutyrate and 1 5% to 6 5% 4 hydroxybutyrate (cell dry weight), depending on precursor supplied. w(monomer) in the terpolyesters varied from 55% to 69% 3 hydroxybutyrate, 0 5% to 3 4% 4 hydroxybutyrate and 3 4% to 16% 3 hydroxyvalerate.
文摘Almost seven decades have elapsed since the establishment of the Chouniugou Formation. This formation used to be considered as a mark for the recognition, division and correlation of the Late Visean biostratigraphic unit in China due to its abundant marine biotas such as gigantoproductids and coral Yuanophyllum. Although many geologists and palaeontologists had reinvestigated the typical section of this formation, they came to
