The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown r...The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown rot of the sweet cherry fruit caused by Monilinia fructicola at 25 and 1 ℃. The populations of yeast increased faster in the presence of the pathogen initially, but then decreased rapidly. In the fruits inoculated with M. fructicola alone or combined with C. laurentii, an induction of lipid peroxidation as well as activities of the antioxidant enzymes, such as, superoxide dismutases （SOD）, catalase （CAT）, and peroxidase （POD）, was observed. The isoenzyme pattern of polypheno/oxidase （PPO） changed greatly after the symptoms appeared, with new PPO isoforms being induced. By contrast, the induction of lipid peroxidation and activities of SOD, CAT, and POD were low, although no significant changes were found in the PPO isoenzyms in the fruits inoculated with antagonist C. laurentii alone. The inhibition of brown rot during the antagonist- pathogen-host interaction in wounds of the sweet cherry fruits was mainly on account of the stimulated growth of C. laurentii as well as the induction of antioxidant enzymes of the fruits by M. fructicola.展开更多
Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits...Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits, were investigated of their biocontrol efficacy against blue mould rot caused by Penicillium expansum Link in apple fruits. T. pullulans was identified to be a new yeast antagonist and was the most effective at inhibiting blue mould rot in the three yeasts. The effects of different concentrations of the three yeasts and addition Of nutrients, as well as combination with calcium on controlling blue mould rot in apples were presented in the paper. The results indicated that the higher the yeast concentrations, the more effective the yeasts on biocontrol activity. Addition of nutrients reduced the biocontrol capacity of the yeasts. Combination of 0.18 mol/L CaCl2 with C. laurentii cell suspensions could significantly enhance its effect to P. expansum in apple fruits, while the efficacy of calcium on biocontrol activity of T. pullulans or R. glutinis was not remarkable.展开更多
以蒜薹为试材,研究了罗伦隐球酵母(Cryptococcus laurentii)对引起蒜薹采后病害的主要病原菌交链孢霉菌(Alternaria Nee ex Wallr.)的生物防治作用。结果表明,在PDB试管中,罗伦隐球酵母菌悬液和培养原液能抑制病原菌孢子的萌发;在蒜薹上...以蒜薹为试材,研究了罗伦隐球酵母(Cryptococcus laurentii)对引起蒜薹采后病害的主要病原菌交链孢霉菌(Alternaria Nee ex Wallr.)的生物防治作用。结果表明,在PDB试管中,罗伦隐球酵母菌悬液和培养原液能抑制病原菌孢子的萌发;在蒜薹上,罗伦隐球酵母菌悬液和培养原液对病原菌有抑制作用;越早接种拮抗菌,抑菌效果越好;适量的有机碳源和有机氮源可以促进罗伦隐球酵母的生长,提高其抑菌效果,而添加无机氮源会降低其抑菌效果。展开更多
基金the Knowledge Innovation Program of the Chinese Academy of Sciences, China （KSCX2-YW-G-010）； the National Natural Science Foundation of China （30671473）.
文摘The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown rot of the sweet cherry fruit caused by Monilinia fructicola at 25 and 1 ℃. The populations of yeast increased faster in the presence of the pathogen initially, but then decreased rapidly. In the fruits inoculated with M. fructicola alone or combined with C. laurentii, an induction of lipid peroxidation as well as activities of the antioxidant enzymes, such as, superoxide dismutases （SOD）, catalase （CAT）, and peroxidase （POD）, was observed. The isoenzyme pattern of polypheno/oxidase （PPO） changed greatly after the symptoms appeared, with new PPO isoforms being induced. By contrast, the induction of lipid peroxidation and activities of SOD, CAT, and POD were low, although no significant changes were found in the PPO isoenzyms in the fruits inoculated with antagonist C. laurentii alone. The inhibition of brown rot during the antagonist- pathogen-host interaction in wounds of the sweet cherry fruits was mainly on account of the stimulated growth of C. laurentii as well as the induction of antioxidant enzymes of the fruits by M. fructicola.
文摘Three antagonistic yeasts, Trichosporon pullulans (Lindner.) Diddens and Lodder, Cryptococcus laurentii (Kuffer.) Skinner and Rhodotorula glutinis (Fresen.) F. C. Harrison, selected from over 50 yeasts on apple fruits, were investigated of their biocontrol efficacy against blue mould rot caused by Penicillium expansum Link in apple fruits. T. pullulans was identified to be a new yeast antagonist and was the most effective at inhibiting blue mould rot in the three yeasts. The effects of different concentrations of the three yeasts and addition Of nutrients, as well as combination with calcium on controlling blue mould rot in apples were presented in the paper. The results indicated that the higher the yeast concentrations, the more effective the yeasts on biocontrol activity. Addition of nutrients reduced the biocontrol capacity of the yeasts. Combination of 0.18 mol/L CaCl2 with C. laurentii cell suspensions could significantly enhance its effect to P. expansum in apple fruits, while the efficacy of calcium on biocontrol activity of T. pullulans or R. glutinis was not remarkable.
文摘以蒜薹为试材,研究了罗伦隐球酵母(Cryptococcus laurentii)对引起蒜薹采后病害的主要病原菌交链孢霉菌(Alternaria Nee ex Wallr.)的生物防治作用。结果表明,在PDB试管中,罗伦隐球酵母菌悬液和培养原液能抑制病原菌孢子的萌发;在蒜薹上,罗伦隐球酵母菌悬液和培养原液对病原菌有抑制作用;越早接种拮抗菌,抑菌效果越好;适量的有机碳源和有机氮源可以促进罗伦隐球酵母的生长,提高其抑菌效果,而添加无机氮源会降低其抑菌效果。
