Objective:To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromcthanc extract of Alseodaphne corneri.Methods:Phytochemical studies of the crude extract led to the i...Objective:To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromcthanc extract of Alseodaphne corneri.Methods:Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography.The antiplasmodial activity of the isolated compounds was evaluated usingthe histidinc-rich protein II assay.The isolated alkaloids were also tested for their antioxidant activity using three different assays:DPPH.ferric reducing ability of plasma and metal chelating assays.Results:Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoplosis.The antioxidant properties of the alkaloids under investigation revealed that in addition to the aniiplasmodial activity,the alkaloids could also prevent oxidative stress.(+)-laurotetanine and(+)-norstephasubine exhibited strong antiplamodial activities with IC_(50) values of 0.189 and 0.116 μM.respectively.Conclusions:Interestingly,the two most potent compounds that exhibit aniiplasmodial activity also exhibit good antioxidant activities.The crude dichloromcthanc extract and the isolated compounds exert substantial aniiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host.展开更多
基金supported by University of Malaya Rescarch Grant(RP001/2012A)(RP001/2012B)postgraduate Research Funds of University of Malaya(PV050/2012A)
文摘Objective:To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromcthanc extract of Alseodaphne corneri.Methods:Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography.The antiplasmodial activity of the isolated compounds was evaluated usingthe histidinc-rich protein II assay.The isolated alkaloids were also tested for their antioxidant activity using three different assays:DPPH.ferric reducing ability of plasma and metal chelating assays.Results:Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoplosis.The antioxidant properties of the alkaloids under investigation revealed that in addition to the aniiplasmodial activity,the alkaloids could also prevent oxidative stress.(+)-laurotetanine and(+)-norstephasubine exhibited strong antiplamodial activities with IC_(50) values of 0.189 and 0.116 μM.respectively.Conclusions:Interestingly,the two most potent compounds that exhibit aniiplasmodial activity also exhibit good antioxidant activities.The crude dichloromcthanc extract and the isolated compounds exert substantial aniiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host.
