Leadership is a complex process.It is one of the most researched areas around the world.It has gained importance in every walk of life from politics to business and from education to social organizations.According to ...Leadership is a complex process.It is one of the most researched areas around the world.It has gained importance in every walk of life from politics to business and from education to social organizations.According to the study of"Leadership in Adult Education Venues",here has a much more clear recognition of leadership:leadership is a process whereby an individual influences a group of individuals to achieve a common goal.There are many approaches of leadership throughout the study of this class,the three theories of leadership I choose to describe in this paper are:Leader-Member Exchange(LMX)Theory,Transformational Leadership,and Team Leadership.展开更多
[ Objective] To express and purify the intracellular hydrophilic domains of bovine membrane carrier proteins：anion exchanger, member 1 （AE1） and electregenic sodium bicarbonate cotransporter 1 （NBCel）, which were...[ Objective] To express and purify the intracellular hydrophilic domains of bovine membrane carrier proteins：anion exchanger, member 1 （AE1） and electregenic sodium bicarbonate cotransporter 1 （NBCel）, which were associated with bicarbonate ion transport. [ Method] The hydrophilic domains of bovine AE1 and NBCel were amplified by PCR and inserted into the prokaryotic expression vector pET-28a, respectively. The recombinant plasmids were transformed into the expression strain E. coli BL21 （DE3） and then induced by IPTG. The expressed proteins were purified by nickel ion affinity chromatography and analyzed by 15% SDS-PAGE. [Result] The hydrophilic domains of bovine AE1 and NBCel were amplified respectively by PCR and expressed by prokaryotic expression system with the induction of IPTG. They were mainly expressed in the cyto- plasm of E. coli and high-purity was achieved by nickel ion affinity chromatography. [Condusion] The expression of the hydrophilic domains of bovine AE1 and NBCel provides a major exit route for preparation of antibodies and the regulatory mechanisms of carrier proteins.展开更多
At the invitation of the Chinese Association for International Understanding (CAFIU), a seven-member young political leader delegation headed by Dong Soo CHUNG, President of National Strategy Institute (NSI) of Republ...At the invitation of the Chinese Association for International Understanding (CAFIU), a seven-member young political leader delegation headed by Dong Soo CHUNG, President of National Strategy Institute (NSI) of Republic of Korea (ROK) visited China from November 1-7. NSI, established in 1991, is composed of personnel from political and economic circles, mainly engaging in research regarding national development strategies and so on. Kang KyongShik,former展开更多
At the invitation of CAFIU,an 11-member European-American young leaders delegation composed of politicians,think tank researchers and media workers from France,Germany,Italy,UK and US headed by Mr.Manlio di Stefano,Me...At the invitation of CAFIU,an 11-member European-American young leaders delegation composed of politicians,think tank researchers and media workers from France,Germany,Italy,UK and US headed by Mr.Manlio di Stefano,Member of House of Representatives of Italy and Chairman of the Five-Star展开更多
Beijing Capital Agribusiness Group(hereinafter referred to as CAG),previously known as Beijing Municipal State Farm Administration,is an industry leader of strong brand competitiveness with 133 thousand hectares of st...Beijing Capital Agribusiness Group(hereinafter referred to as CAG),previously known as Beijing Municipal State Farm Administration,is an industry leader of strong brand competitiveness with 133 thousand hectares of state-owned land,40000 employees and a total asset of 50展开更多
This study examines how and when authoritarian leadership affects subordinates’task performance.Using social exchange theory and power dependence theory,this study proposes that authoritarian leadership negatively in...This study examines how and when authoritarian leadership affects subordinates’task performance.Using social exchange theory and power dependence theory,this study proposes that authoritarian leadership negatively influences task performance through leader-member exchange(LMX).This study further proposes that the effect of authoritarian leadership on LMX is stronger when a subordinate has less dependence on a leader.A two-wave survey was conducted in a large electronics and information enterprise group in China.These hypotheses are supported by results based on 219 supervisor-subordinate dyads.The results reveal that authoritarian leadership negatively affects subordinates’task performance via LMX.Dependence on leader buffers the negative effect of authoritarian leadership on LMX and mitigates the indirect effect of authoritarian leadership on employee task performance through LMX.Theoretical contributions and practical implications are discussed.展开更多
文摘Leadership is a complex process.It is one of the most researched areas around the world.It has gained importance in every walk of life from politics to business and from education to social organizations.According to the study of"Leadership in Adult Education Venues",here has a much more clear recognition of leadership:leadership is a process whereby an individual influences a group of individuals to achieve a common goal.There are many approaches of leadership throughout the study of this class,the three theories of leadership I choose to describe in this paper are:Leader-Member Exchange(LMX)Theory,Transformational Leadership,and Team Leadership.
基金financially supported by Key Project of Jiangsu Science and Technology Department (BC2004365)
文摘[ Objective] To express and purify the intracellular hydrophilic domains of bovine membrane carrier proteins：anion exchanger, member 1 （AE1） and electregenic sodium bicarbonate cotransporter 1 （NBCel）, which were associated with bicarbonate ion transport. [ Method] The hydrophilic domains of bovine AE1 and NBCel were amplified by PCR and inserted into the prokaryotic expression vector pET-28a, respectively. The recombinant plasmids were transformed into the expression strain E. coli BL21 （DE3） and then induced by IPTG. The expressed proteins were purified by nickel ion affinity chromatography and analyzed by 15% SDS-PAGE. [Result] The hydrophilic domains of bovine AE1 and NBCel were amplified respectively by PCR and expressed by prokaryotic expression system with the induction of IPTG. They were mainly expressed in the cyto- plasm of E. coli and high-purity was achieved by nickel ion affinity chromatography. [Condusion] The expression of the hydrophilic domains of bovine AE1 and NBCel provides a major exit route for preparation of antibodies and the regulatory mechanisms of carrier proteins.
文摘At the invitation of the Chinese Association for International Understanding (CAFIU), a seven-member young political leader delegation headed by Dong Soo CHUNG, President of National Strategy Institute (NSI) of Republic of Korea (ROK) visited China from November 1-7. NSI, established in 1991, is composed of personnel from political and economic circles, mainly engaging in research regarding national development strategies and so on. Kang KyongShik,former
文摘At the invitation of CAFIU,an 11-member European-American young leaders delegation composed of politicians,think tank researchers and media workers from France,Germany,Italy,UK and US headed by Mr.Manlio di Stefano,Member of House of Representatives of Italy and Chairman of the Five-Star
文摘Beijing Capital Agribusiness Group(hereinafter referred to as CAG),previously known as Beijing Municipal State Farm Administration,is an industry leader of strong brand competitiveness with 133 thousand hectares of state-owned land,40000 employees and a total asset of 50
基金the National Natural Science Foundation of China(No.71971211)the Humanity and Social Science Youth Foundation of Ministry of Education of China(18YJC630192).
文摘This study examines how and when authoritarian leadership affects subordinates’task performance.Using social exchange theory and power dependence theory,this study proposes that authoritarian leadership negatively influences task performance through leader-member exchange(LMX).This study further proposes that the effect of authoritarian leadership on LMX is stronger when a subordinate has less dependence on a leader.A two-wave survey was conducted in a large electronics and information enterprise group in China.These hypotheses are supported by results based on 219 supervisor-subordinate dyads.The results reveal that authoritarian leadership negatively affects subordinates’task performance via LMX.Dependence on leader buffers the negative effect of authoritarian leadership on LMX and mitigates the indirect effect of authoritarian leadership on employee task performance through LMX.Theoretical contributions and practical implications are discussed.
