Pathogenic Identification of Leaf Tip Blight of Red-fleshed Kiwifruit in Qiandongnan Prefecture

[Objective]The paper was to investigate and identify new diseases in kiwifruit producing areas in Qiandongnan Prefecture to reduce the harm of diseases and ensure the quality of red-fleshed kiwifruit products.[Method]The pathogenic fungus was isolated from diseased leaves of redfleshed kiwifruit by tissue separation method,and DNA was sequenced by ribosomal rDNA-ITS(internal transcribed spacer)sequencing.Molecular evolutionary trees were built using MEGA4.0 software,and the pathogenic fungus was classified and identified combined with morphological obser-vation.[Result]Leaf tip blight was a new disease caused by Epicoccum sorghinum.It caused serious damage on red-fleshed kiwifruit.[Conclusion]The study supplements diseases of red-fleshed kiwifruit,and provides support for disease prevention and control in late stage.

Identification of Pathogens on Dry Blight Disease in Leaf Tip of Red-fleshed Kiwifruit in Qiandongnan Prefecture

In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried out. The pathogen was isolated from diseased leaves by a tissue separation method. DNA sequencing was performed by the sequence analysis of ribosomal rDNA-ITS(Internal transcribed spacer) region, and a molecular evolutionary tree was constructed by the MEGA 4.0 software. The pathogenic fungus was classified and identified by combining morphological observation finally. The results showed that the dry blight disease in leaf tip was a new disease, which was caused by Epicoccum sorghinum, and it had a serious damage to red-fleshed kiwifruit.

不同牡丹品种对大气氟污染的抗性测定

以污染区牡丹叶尖枯病的病情指数为依据，测定了１２８个牡丹品种对大气氟污染的抗性。结果表明，不同牡丹品种对大气氟污染的抗性不同，抗性指数的变化范围为２０～１００．抗性差异是由于叶片对氟化物的耐受性不同，而不是吸氟量多少的差异。

牡丹叶尖枯病的症状和发生原因

根据牡丹叶尖枯病的症状特点和田间扩展方式，以及对大气和牡丹叶片中氟浓度的测定，结果证实牡丹叶尖枯病是由大气氟污染引起的一种非侵染性病害。污染区的牡丹在生长季节叶片不断地吸收氟化物，当叶片中氟浓度超过了其能够耐受的限度时，即表现枯死症状。由于氟化物在叶片中会随着蒸腾流向叶尖，而使叶尖的氟浓度较高，故枯死症状先在叶尖出现。

国家林木种质资源平台凯里基地植物病虫害的种类调查和病原鉴定(二)

该文是2015年10月国家林木种质资源平台—凯里基地病虫害调查和病原鉴定的后续结果。调查发现的红花檵木Loropetalum chinense炭疽病、绣球花Hydrangea macrophylla炭疽病和竹柏Podocarpus nagi叶尖枯病的病原菌分别为胶胞炭疽菌Colletotrichum gloeosporioide、互隔文链孢霉Alternaria alternate和头状茎点霉Phoma glomerata。

大气中氟污染对牡丹影响的调查分析

对大气氟污染与观赏植物牡丹叶片中的含氟量之间的关系进行了分析。结果表明 :( 1 )一定时间内牡丹叶片的吸氟量和积累量成正相关。 ( 2 )牡丹叶片中的含氟量与测定点距污染源的距离呈负相关。 ( 3 )牡丹叶尖枯病是因大气氟污染所致 。

黔东南州红心猕猴桃叶尖干枯病病原鉴定

为减少黔东南州的红心猕猴桃病害危害、保证产品质量,对产区内的新病害发生情况进行调查研究及病原鉴定。采用组织分离法对病叶进行病原菌分离,使用核糖体rDNA-ITS(Internal transcribed spacer)区序列分析法进行DNA测序,使用MEGA 4.0软件构建分子进化树,结合形态学观察对致病菌进行分类鉴定。结果表明,由病原菌高粱附球菌(Epicoccum sorghinum)导致的一种新病害——叶尖干枯病对红心猕猴桃有严重危害。