Correlation and Pathway Analysis of the Carbon,Nitrogen,and Phosphorus in Soil-Microorganism-Plant with Main Quality Components of Tea(Camellia sinensis)

The contents of carbon(C),nitrogen(N),and phosphorus(P)in soil-microorganisms-plant significantly affect tea quality by altering the main quality components of tea,such as tea polyphenols,amino acids,and caffeine.However,few studies have quantified the effects of these factors on the main quality components of tea.The study aimed to explore the interactions of C,N,and P in soil-microorganisms-plants and the effects of these factors on the main quality components of tea by using the path analysis method.The results indicated that(1)The contents of C,N,and P in soil,microorganisms,and tea plants were highly correlated and collinear,and showed significant correlations with the main quality components of tea.(2)Optimal regression equations were established to esti-mate tea polyphenol,amino acid,catechin,caffeine,and water extract content based on C,N,and P contents in soil,microorganisms,and tea plants(R^(2)=0.923,0.726,0.954,0.848,and 0.883,respectively).(3)Pathway analysis showed that microbial biomass phosphorus(MBP),root phosphorus,branch nitrogen,and microbial biomass carbon(MBC)were the largest direct impact factors on tea polyphenol,catechin,water extracts,amino acid,and caffeine content,respectively.Leaf carbon,root phosphorus,and leaf nitrogen were the largest indirect impact factors on tea polyphenol,catechin,and water extract content,respectively.Leaf carbon indirectly affected tea polyphenol content mainly by altering MBP content.Root phosphorus indirectly affected catechin content mainly by altering soil organic carbon content.Leaf nitrogen indirectly affected water extract content mainly by altering branch nitrogen content.The research results provide the scientific basis for reasonable fertilization in tea gardens and tea quality improvement.

Effects of tree size and organ age on variations in carbon,nitrogen,and phosphorus stoichiometry in Pinus koraiensis

Carbon(C),nitrogen(N),and phosphorus(P)are of fundamental importance for growth and nutrient dynamics within plant organs and deserve more attention at regional to global scales.However,our knowledge of how these nutrients vary with tree size,organ age,or root order at the individual level remains limited.We determined C,N,and P contents and their stoichiometric ratios(i.e.,nutrient traits)in needles,branches,and fine roots at different organ ages(0-3-year-old needles and branches)and root orders(1st-4th order roots)from 64 Pinus koraiensis of varying size(Diameter at breast height ranged from 0.3 to 100 cm)in northeast China.Soil factors were also measured.The results show that nutrient traits were regulated by tree size,organ age,or root order rather than soil factors.At a whole-plant level,nutrient traits decreased in needles and fine roots but increased in branches with tree size.At the organ level,age or root order had a negative effect on C,N,and P and a positive effect on stoichiometric ratios.Our results demonstrate that nutrient variations are closely related to organ-specific functions and ecophysiological processes at an individual level.It is suggested that the nutrient acquisition strategy by younger trees and organ fractions with higher nutrient content is for survival.Conversely,nutrient storage strategy in older trees and organ fractions are mainly for steady growth.Our results clarified the nutrient utilization strategies during tree and organ ontogeny and suggest that tree size and organ age or root order should be simultaneously considered to understand the complexities of nutrient variations.

Degree of shade tolerance shapes seasonality of chlorophyll, nitrogen and phosphorus levels of trees and herbs in a temperate deciduous forest

Forest productivity is closely linked to seasonal variations and vertical differentiation in leaf traits.However,leaf structural and chemical traits variation among co-existing species,and plant functional types within the canopy are poorly quantified.In this study,the seasonality of leaf chlorophyll,nitrogen(N),and phosphorus(P)were quantified vertically along the canopy of four major tree species and two types of herbs in a temperate deciduous forest.The role of shade tolerance in shaping the seasonal variation and vertical differentiation was examined.During the entire season,chlorophyll content showed a distinct asymmetric unimodal pattern for all species,with greater chlorophyll levels in autumn than in spring,and the timing of peak chlorophyll per leaf area gradually decreased as shade tolerance increased.Chlorophyll a:b ratios gradually decreased with increasing shade tolerance.Leaf N and P contents sharply declined during leaf expansion,remained steady in the mature stage and decreased again during leaf senescence.Over the seasons,the lower canopy layer had significantly higher chlorophyll per leaf mass but not chlorophyll per leaf area than the upper canopy layer regardless of degree of shade tolerance.However,N and P per leaf area of intermediate shade-tolerant and fully shade-tolerant tree species were significantly higher in the upper canopy than in the lower.Seasonal variations in N:P ratios suggest changes in N or P limitation.These findings indicate that shade tolerance is a key feature shaping inter-specific differences in leaf chlorophyll,N,and P contents as well as their seasonality in temperate deciduous forests,which have significant implications for modeling leaf photosynthesis and ecosystem production.

Carbon,nitrogen and phosphorus stoichiometry in Pinus tabulaeformis forest ecosystems in warm temperate Shanxi Province,north China

Although carbon(C), nitrogen(N), and phosphorous(P) stoichiometric ratios are considered good indicators of nutrient excess/limitation and thus of ecosystem health, few reports have discussed the trends and the reciprocal effects of C:N:P stoichiometry in plant–litter–soil systems. The present study analyzed C:N:P ratios in four age groups of Chinese pine, Pinus tabulaeformis Carr., forests in Shanxi Province, China: plantation young forests(AY,<20 year-old); plantation middle-aged forests(AM, 21–30 year-old); natural young forests(NY,<30 year-old); and natural middle-aged forests(NM,31–50 year-old). The average C:N:P ratios calculated for tree, shrub, and herbaceous leaves, litter, and soil(0–100 cm) were generally higher in NY followed by NM,AM, and AY. C:N and C:P ratios were higher in litter than in leaves and soils, and reached higher values in the litter and leaves of young forests than in middle-aged forests;however, C:N and C:P ratios were higher in soils of middle-aged forests than in young forests. N:P ratios were higher in leaves than in litter and soils regardless of stand age; the consistent N:P<14 values found in all forests indicated N limitations. With plant leaves, C:P ratios were highest in trees, followed by herbs and shrubs, indicating a higher efficiency in tree leaf formation. C:N ratios decreased with increasing soil depth, whereas there was no trend for C:P and N:P ratios. C:N:P stoichiometry of forest foliage did not exhibit a consistent variation according to stand age. Research on the relationships between N:P, and P, N nutrient limits and the characteristics of vegetation nutrient adaptation need to be continued.

Cu_(3)P nanoparticles confined in nitrogen/phosphorus dual-doped porous carbon nanosheets for efficient potassium storage

Immobilizing primary electroactive nanomaterials in porous carbon matrix is an effective approach for boosting the electrochemical performance of potassium-ion batteries (PIBs) because of the synergy among functional components. Herein, an integrated hybrid architecture composed of ultrathin Cu_(3)P nanoparticles (~20 nm) confined in porous carbon nanosheets (Cu_(3)P⊂NPCSs) as a new anode material for PIBs is synthesized through a rational self-designed self-templating strategy. Benefiting from the unique structural advantages including more active heterointerfacial sites, intimate and stable electrical contact, effectively relieved volume change, and rapid K^(+) ion migration, the Cu_(3)P⊂NPCSs indicate excellent potassium-storage performance involving high reversible capacity, exceptional rate capability, and cycling stability. Moreover, the strong adsorption of K^(+) ions and fast potassium-ion reaction kinetics in Cu_(3)P⊂NPCSs is verified by the theoretical calculation investigation. Noted, the intercalation mechanism of Cu_(3)P to store potassium ions is, for the first time, clearly confirmed during the electrochemical process by a series of advanced characterization techniques.

Solar radiation effects on leaf nitrogen and phosphorus stoichiometry of Chinese fir across subtropical China

Background:Solar radiation(SR)plays critical roles in plant physiological processes and ecosystems functions.However,the exploration of SR influences on the biogeochemical cycles of forest ecosystems is still in a slow progress,and has important implications for the understanding of plant adaption strategy under future environmental changes.Herein,this research was aimed to explore the influences of SR on plant nutrient characteristics,and provided theoretical basis for introducing SR into the establishment of biochemical models of forest ecosystems in the future researches.Methods:We measured leaf nitrogen(N)and phosphorus(P)stoichiometry in 19 Chinese fir plantations across subtropical China by a field investigation.The direct and indirect effects of SR,including global radiation(Global R),direct radiation(Direct R)and diffuse radiation(Diffuse R)on the leaf N and P stoichiometry were investigated.Results:The linear regression analysis showed that leaf N concentration had no association with SR,while leaf P concentration and N:P ratio were negatively and positively related to SR,respectively.Partial least squares path model(PLS-PM)demonstrated that SR(e.g.Direct R and Diffuse R),as a latent variable,exhibited direct correlations with leaf N and P stoichiometry as well as the indirect correlation mediated by soil P content.The direct associations(path coefficient=−0.518)were markedly greater than indirect associations(path coefficient=−0.087).The covariance-based structural equation modeling(CB-SEM)indicated that SR had direct effects on leaf P concentration(path coefficient=−0.481),and weak effects on leaf N concentration.The high SR level elevated two temperature indexes(mean annual temperature,MAT;≥10°C annual accumulated temperature,≥10℃ AAT)and one hydrological index(mean annual evapotranspiration,MAE),but lowered the soil P content.MAT,MAE and soil P content could affect the leaf P concentration,which cause the indirect effect of SR on leaf P concentration(path coefficient=0.004).Soil N content had positive effect on the leaf N concentration,which was positively and negatively regulated by MAP and≥10℃ AAT,respectively.Conclusions:These results confirmed that SR had negatively direct and indirect impacts on plant nutrient status of Chinese fir based on a regional investigation,and the direct associations were greater than the indirect associations.Such findings shed light on the guideline of taking SR into account for the establishment of global biogeochemical models of forest ecosystems in the future studies.

Carbon,nitrogen and phosphorus coupling relationships and their influencing factors in the critical zone of Dongting Lake wetlands,China

Wetland is a transition zone between terrestrial and aquatic ecosystems,and is the source and sink of various biogenic elements in the earth’s epipelagic zone.In order to investigate the driving force and coupling mechanism of carbon(C),nitrogen(N)and phosphorus(P)migration in the critical zone of lake wetland,this paper studies the natural wetland of Dongting Lake area,through measuring and analysing the C,N and P contents in the wetland soil and groundwater.Methods of Pearson correlation,non-linear regression and machine learning were employed to analyse the influencing factors,and to explore the coupling patterns of the C,N and P in both soils and groundwater,with data derived from soil and water samples collected from the wetland critical zone.The results show that the mean values of organic carbon(TOC),total nitrogen(TN)and total phosphorus(TP)in groundwater are 1.59 mg/L,4.19 mg/L and 0.5 mg/L,respectively,while the mean values of C,N and P in the soils are 18.05 g/kg,0.86 g/kg and 0.52 g/kg.The results also show that the TOC,TN and TP in the groundwater are driven by a variety of environmental factors.However,the concentrations of C,N and P in the soils are mainly related to vegetation abundance and species which influence each other.In addition,the fitted curves of wetland soil C-N and C-P appear to follow the power function and S-shaped curve,respectively.In order to establish a multivariate regression model,the soil N and P contents were used as the input parameters and the soil C content used as the output one.By comparing the prediction effects of machine learning and nonlinear regression modelling,the results show that coupled relationship equation for the C,N and P contents is highly reliable.Future modelling of the coupled soil and groundwater elemental cycles needs to consider the complexity of hydrogeological conditions and to explore the quantitative relationships among the influencing factors and chemical constituents.

Twice-split phosphorus application alleviates low-temperature impacts on wheat by improved spikelet development and setting

Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged.

How <i>Betula ermanii</i>Maintains a Positive Carbon Balance at the Individual Leaf Level at High Elevations

Generally, plant species with shorter leaf longevity maintain a positive carbon balance by decreasing leaf mass per area (LMA) and increasing photosynthesis. However, plants at high elevations need to increase LMA against environmental stresses. Therefore, plants need to increase both LMA and photosynthesis at high elevations. To examine how deciduous plants maintain a positive carbon balance at high elevations, photosynthesis and related leaf traits for deciduous broad-leaved tree Betula ermanii were measured at three elevations. LMA was greater at middle and high elevations than at low elevation. Leaf δ13C was greater at higher elevations, and positively correlated with LMA, indicating greater long-term deficiency of CO2 in leaves at higher elevations. However, the Ci/Ca ratio at photosynthetic measurement was not low at high elevations. Nitrogen content per leaf mass and stomatal conductance were greater at higher elevations. Photosynthetic rates and photosynthetic nitrogen use efficiency (PNUE) did not differ among the three elevations. Photosynthetic rate showed a strong positive correlation with stomatal conductance on a leaf area basis (R2 = 0.83, P < 0.001). Therefore, this study suggests B. ermanii compensates the deficiency of CO2 in leaves at high elevation by increasing stomatal conductance, and maintains photosynthesis and PNUE at high elevation as much as at low elevation.

不同温度下杉木凋落叶分解过程中碳氮磷释放及其化学计量比变化

[目的]研究升温对不同发育阶段杉木凋落叶分解过程中碳(C)、氮(N)、磷(P)释放规律及其生态化学计量特征的影响,揭示气候变暖背景下杉木人工林凋落叶分解过程中养分释放特征。[方法]收集中龄林(18年生)、成熟林(30年生)和过熟林(42年生)3个发育阶段的杉木凋落叶,设置25、30和35℃3个温度梯度进行室内模拟分解试验。[结果](1)在264 d的分解周期内,各发育阶段杉木凋落叶C、N、P残留率总体表现为随分解时间的增加而减小,但不同元素残留率变化模式不同,C残留率表现为释放—富集—释放模式,N残留率表现为富集—释放模式,P残留率表现为释放—富集模式。(2)拟合模型结果表明,成熟林与过熟林凋落叶分解过程中N周转期在35℃处理下比25℃处理分别缩短了34.4%和16.9%,P周转期分别缩短了38.4%和43.8%。(3)不同发育阶段杉木凋落叶分解过程中C:N、N:P比总体呈波动变化,C:P比呈先增大后减小的变化趋势,杉木凋落叶C:N、C:P、N:P比变幅分别为9.32~39.0、949~2194、32.7~153,升温处理在凋落叶分解过程总体上增大了C:P、N:P,降低了C:N比。[结论]温度升高能够缩短成熟林、过熟林凋落叶分解过程中N、P的周转期,提高各发育阶段杉木凋落叶分解过程中的C:P、N:P,说明杉木凋落叶分解明显受P限制,建议根据不同发育阶段杉木的生长需求,适当增加磷肥的施用。

赖草草地叶片养分、碳组分和防御性化合物对氮添加的响应

本研究依托山西右玉黄土高原赖草(Leymus secalinus)草地2017年建立的氮(Nitrogen, N)添加梯度试验平台(0~32 g·m^(-2)·a^(-1)),探究N添加对优势植物赖草叶片养分[N、磷(Phosphorus, P)、钾(Potassium, K)]、非结构性碳水化合物、结构性碳水化合物和防御性化合物含量的影响。结果表明:N素输入显著提高叶片N,K含量和N∶K和N∶P比值,降低叶片P含量和P∶K比值;N素输入显著降低叶片非结构性碳水化合物和结构性碳水化合物含量;N素输入显著增加叶片总酚、单宁和黄酮类化合物含量;PCA分析表明低N(≤8 g·m^(-2)·a^(-1))和高N(>8 g·m^(-2)·a^(-1))输入下赖草叶片性状存在显著差异,土壤无机N含量、土壤有效N∶P和N∶K是其主要影响因子。以上结果表明N素输入改变了盐渍化草地赖草叶片养分-碳组分-防御性化合物的分配策略。

基于δ^(13)C估算水分利用效率的2种模型比较——以峨眉山雷洞坪植物为例

【目的】通过对基于碳稳定同位素值(δ^(13)C)估算水分利用效率(iWUE)的2种模型(是否考虑叶肉导度,gm)之间差异的研究,有助于进一步理解gm对植物iWUE的影响。【方法】选择峨眉山雷洞坪针阔混交林中不同生长型植物,采集了48个物种的117个叶片样品,通过测定其叶片δ^(13)C,对比了早期估算iWUE模型(iWUEsim模型,将gm看作无穷大)与包含gm效应的iWUE模型(iWUEmes模型)之间的iWUE差异(iWUE_(difference)),以及这种差异随植物生长型变化的趋势。【结果】结果显示,iWUEsim的平均值(42.23±1.33μmol·mol^(-1))显著高于iWUEmes的平均值(28.10±0.65μmol·mol^(-1)),高估比例为3.64%~72.11%(平均49.58%±1.30%)。不同生长型植物的iWUEsim均显著大于iWUEmes,其中草本植物高估比例3.64%~48.00%(平均29.20%±5.16%),灌木高估比例13.68%~67.73%(平均48.44%±1.83%),乔木高估比例32.04%~72.11%(平均54.08%±1.47%)。此外,叶片功能性状对iWUE_(difference)有显著影响,相比于叶片厚度、叶片干物质含量和单位质量叶片氮含量,单位面积叶片氮含量和比叶重是影响iWUE_(difference)变化的主要因素。【结论】将gm看作无穷大会造成对基于δ^(13)C估算的iWUE的高估,未来估算植物iWUE时应考虑gm的影响。

Phosphorus/nitrogen co-doped hollow carbon fibers enabling high-rate potassium storage

Potassium-ion hybrid capacitors(PIHCs)reconcile the advantages of batteries and supercapacitors,exhibiting both good energy density and high-power density.However,the low-rate performance and poor cycle stability of battery-type anodes hinder their practical application.Herein,phosphorus/nitrogen co-doped hollow carbon fibers(P-HCNFs)are prepared by a facile template method.The stable grape-like structure with continuous and interconnected cavity structure is an ideal scaffold for shortening the ion transport and relieving volume expansion,while the introduction of P atoms and intrinsic N atoms can create abundant extrinsic/intrinsic defects and additional active sites,reducing the K+diffusion barrier and improving the capacitive-controlled capacity.The P-HCNFs delivers a high specific capacity of 310 mAh·g^(-1)at 0.1 A·g^(-1)with remarkable ultra-high-rate performance(140 mAh·g^(-1)at 50 A·g^(-1))and retains an impressive capacity retention of 87%after 10,000 cycles at 10 A·g^(-1).As expected,the as-assembled PIHCs present a high energy density(115.8 Wh·kg^(-1)at 378.0 W·kg^(-1))and excellent capacity retention of 91%after 20,000 cycles.This work not only shows great potential for utilizing heteroatom-doping and structural design strategies to boost potassium storage,but also paves the way for advancing the practicality of high-energy PIHCs devices.

载畜率对冷蒿不同根际距离土壤细菌及其碳氮含量的影响

为了探究荒漠草原不同载畜率下半灌木植物冷蒿(Artemisia frigida)根、茎、叶碳氮含量与其根际、非根际土壤细菌群落结构的关系,利用高通量测序技术分析土壤(根际和非根际)细菌多样性和群落组成,测定根、茎、叶碳氮含量,分析细菌多样性与根茎叶碳氮含量的关系。结果表明:不同载畜率对冷蒿根茎叶的碳氮含量存在显著影响,中度和重度放牧会限制冷蒿的生长发育。不同载畜率下,根际土壤属水平下细菌的相对丰度均高于非根际土壤,根际与非根际土壤细菌Simpson指数、ACE指数均存在显著性差异(P<0.05)。冷蒿根茎叶碳氮含量与土壤中红色杆菌属和亚硝化球菌属的相对丰度存在相关性关系。

Sulfur-encapsulated in heteroatom-doped hierarchical porous carbon derived from goat hair for high performance lithium–sulfur batteries

Biomass-derived carbon materials have aroused widespread concern as host material of sulfur to enhance electrochemical performances for lithium–sulfur batteries. Herein, goat hair, as a low-cost and eco-friendly precursor, is employed to fabricate cauliflower-like in-situ nitrogen, oxygen and phosphorus tri-doped porous biomass carbon(NOPC) by a facile activation with H_3PO_4 and carbonization process.The morphology and microstructure of NOPC can be readily tuned by altering pyrolysis temperature. The as-prepared NOPC matrix material carbonized at 600 °C possesses 3D hierarchical porous structure, high specific surface area(535.352 m^2 g^(-1)), and appropriate pore size and pore size distribution. Encapsulating sulfur into the NOPC depends on a stem-melting technology as cathode materials of Li–S batteries. Due to the synergistic effect of special physical structure and inherent tri-doping of N, O and P, electrons and ions transfer and utilization of active sulfur in the materials are improved, and the shuttle behaviors of soluble lithium polysulfides are also mitigated. Consequently, the S/NOPC-600 composite exhibits excellent electrochemical performance, giving a high initial discharge capacity of 1185 mA h g^(-1) at 0.05 C and maintaining a relatively considerable capacity of 489 m A h g^(-1) at 0.2 C after 300 cycles. Our work shows that a promising candidate for cathode material of Li–S batteries can be synthesized using low-cost and renewable biomass materials by a facile process.

Soil Organic Carbon and Nutrients along an Alpine Grassland Transect across Northern Tibet

Soil carbon and nutrient contents and their importance in advancing our understanding of biogeochemical cycling in terrestrial ecosystem, has motivated ecologists to find their spatial patterns in various geographical area. Few studies have focused on changes in the physical and chemical properties of soils at high altitudes. Our aim was to identify the spatial distribution of soil physical and chemical properties in cold and arid climatic region. We also tried to explore relationship between soil organic carbon （SOC） and total nitrogen （TN）, total phosphorus （TP）, available nitrogen （AN）, available phosphorus （AP）, soil particle size distribution （PSD）. Samples were collected at 44 sites along a 300 km transect across the alpine grassland of northern Tibet. The study results showed that grassland type was the main factor influencing SOC, TN and TP distribution along the Gangdise Mountain-Shenzha-Shuanghu Transect. SOC, TN and TP contents were significantly higher in alpine meadow than alpine steppe ecosystems. SOC, TN, TP and AN contents in two soil layers （0-15 cm and 15-3o cm） showed no significant differences, while AP content in top soft （0-15 cm） was significantly higher than that in sub-top soil （15-30cm）. SOC content was correlated positively with TN and TP content （r = 0.901and 0.510, respectively）. No correlations were detected for clay content and fractal dimension of particle size distribution （D）. Our study results indicated the effects of vegetation on soil C, N and P seem to be more important than that of rocks itself along latitude gradient on the northern Tibetan Plateau. However, we did not found similar impacts of vegetation on soil properties in depth. Inaddition, this study also provided an interesting contribution to the global data pool on soil carbon stocks.

Effects of ontogenetic stage and leaf age on leaf functional traits and the relationships between traits in Pinus koraiensis

Investigating the effects of ontogenetic stage and leaf age on leaf traits is important for understanding the utilization and distribution of resources in the process of plant growth.However,few studies have been conducted to show how traits and trait-trait relationships change across a range of ontogenetic stage and leaf age for evergreen coniferous species.We divided 67 Pinus koraiensis Sieb.et Zucc.of various sizes(0.3-100 cm diameter at breast height,DBH)into four ontogenetic stages,i.e.,young trees,middle-aged trees,mature trees and over-mature trees,and measured the leaf mass per area(LMA),leaf dry matter content(LDMC),and mass-based leaf nitrogen content(N)and phosphorus content(P)of each leaf age group for each sampled tree.One-way analysis of variance(ANOVA)was used to describe the variation in leaf traits by ontogenetic stage and leaf age.The standardized major axis method was used to explore the effects of ontogenetic stage and leaf age on trait-trait relationships.We found that LMA and LDMC increased significantly and N and P decreased significantly with increases in the ontogenetic stage and leaf age.Most trait-trait relationships were consistent with the leaf economic spectrum(LES)at a global scale.Among them,leaf N content and LDMC showed a significant negative correlation,leaf N and P contents showed a significant positive correlation,and the absolute value of the slopes of the trait-trait relationships showed a gradually increasing trend with an increasing ontogenetic stage.LMA and LDMC showed a significant positive correlation,and the slopes of the trait-trait relationships showed a gradually decreasing trend with leaf age.Additionally,there were no significant relationships between leaf N content and LMA in most groups,which is contrary to the expectation of the LES.Overall,in the early ontogenetic stages and leaf ages,the leaf traits tend to be related to a"low investment-quick returns"resource strategy.In contrast,in the late ontogenetic stages and leaf ages,they tend to be related to a"high investment-slow returns"resource strategy.Our results reflect the optimal allocation of resources in Pinus koraiensis according to its functional needs during tree and leaf ontogeny.

滴灌减氮对四川盆地玉米吐丝后叶片衰老和物质积累的影响

为研究滴灌减氮对玉米吐丝后叶片衰老特性、碳氮代谢及物质积累的影响,设置正常施氮(240 kg·hm^(-2),N 240)和减氮25%(180 kg·hm^(-2),N 180)2个氮肥量和4个滴灌水平(0 m^(3)·hm^(-2),B 0;375 m^(3)·hm^(-2),B_(1);750 m^(3)·hm^(-2),B_(2);1125 m^(3)·hm^(-2),B_(3)),并以不施氮且不滴灌为对照组(CK),分析比较了不同氮肥水平下滴灌对玉米吐丝后穗位叶叶绿素含量、保护酶(超氧化物歧化酶,SOD;过氧化物酶,POD;过氧化氢酶,CAT)和碳氮代谢酶(蔗糖合成酶,SS;谷氨酰胺合成酶,GS)活性及物质积累的影响。结果表明:玉米吐丝后穗位叶叶绿素含量、保护酶和碳氮代谢酶活性以及吐丝后物质积累量均随施氮量和滴灌量增加而增加,且二者间存在一定互作效应。N 180 B 0较N 240 B 0处理各测定时期的平均叶绿素含量、SOD、POD、CAT、SS和GS活性及花后干物质积累量分别降低19.01%、19.71%、10.55%、7.32%、10.91%、19.91%和4.46%,但N 180 B_(3)处理的上述指标与N 240 B 0差异不显著甚至高于N 240 B 0,表明氮肥水平和滴灌量间存在一定协同作用和互补效应,N 180 B_(3)处理能实现延缓叶片衰老、维持较强的碳氮代谢能力。研究结果可为四川盆地玉米水肥一体化减氮增效技术提供理论依据。

骏枣叶片碳氮营养与成花关系研究

【目的】探究骏枣叶片碳氮营养变化与成花的关系,为枣花期调控和提高枣花芽质量提供科学依据。【方法】对新疆阿拉尔市8年生骏枣不同花期阶段叶片中碳水化合物、含氮化合物、叶片叶绿素含量和枣吊干物质积累进行测定,分析成花与枣吊叶片碳氮营养和生长的关系。【结果】枣树成花进程中叶片各营养成分呈一定规律变化,初花期叶片碳水化合物和含氮化合物积累达到高峰,进入盛花期后总糖、淀粉、还原糖和蛋白质含量均明显下降;叶片中蔗糖仅在现蕾期有较大增长,盛花期下降不明显;氨基酸含量仅在盛花期有明显增长;初花期叶片C/N达峰值,进入盛花期后明显下降;叶绿素含量在未现蕾期至初花期快速升高,后期维持在较高水平;枣吊干重在进入初花期后快速增长,进入盛花期后单枝枣吊上叶片数基本不变,枣吊上叶片干重和枣吊长度增长变缓。【结论】枣吊叶片淀粉和蛋白质积累是枣成花的重要物质基础,盛花期枣吊叶片代谢旺盛,碳氮营养形态快速转变,并促进碳素营养向花器官运输。

Nitrogen-,phosphorus-doped carbon-carbon nanotube CoP dodecahedra by controlling zinc content for high-performance electrocatalytic oxygen evolution

Here,N-and P-doped carbon-carbon nanotube CoP(NPC-CNTs-CoP)nanoparticles dodecahedra are achieved by multistep calcination of the Zn-doped zeolitic imidazolate framework ZIF-67 precursor(ZnCo-ZIF).In the structures,the presence of N and P atoms,abundant CNTs and the CoP nanoparticles can enhance electrochemical activity and promote the structural stability of materials.As the temperature increases,the Zn contents gradually reduce to zero,which provides more active sites for electrochemical testing.Furthermore,the high specific surface area and microporous behavior of NPC-CNTsCoP-9 make it excellent in electrocatalytic testing.NPCCNTs-CoP-9 shows a low overpotential of 224 mV at10 mA·cm^-2 in 1.0 mol·L^-1 KOH solution.The strategy of zeolitic imidazole framework-derived transition metal phosphides will provide a new sight for developing energy conversion materials.