Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wa...Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wavelet,feature-fused,pre-trained deep learning model for detecting olive leaf diseases.The proposed model combines wavelet transforms with pre-trained deep-learning models to extract discriminative features from olive leaf images.The model has four main phases:preprocessing using data augmentation,three-level wavelet transformation,learning using pre-trained deep learning models,and a fused deep learning model.In the preprocessing phase,the image dataset is augmented using techniques such as resizing,rescaling,flipping,rotation,zooming,and contrasting.In wavelet transformation,the augmented images are decomposed into three frequency levels.Three pre-trained deep learning models,EfficientNet-B7,DenseNet-201,and ResNet-152-V2,are used in the learning phase.The models were trained using the approximate images of the third-level sub-band of the wavelet transform.In the fused phase,the fused model consists of a merge layer,three dense layers,and two dropout layers.The proposed model was evaluated using a dataset of images of healthy and infected olive leaves.It achieved an accuracy of 99.72%in the diagnosis of olive leaf diseases,which exceeds the accuracy of other methods reported in the literature.This finding suggests that our proposed method is a promising tool for the early detection of olive leaf diseases.展开更多
Banana is a significant crop,and three banana leaf diseases,including Sigatoka,Cordana and Pestalotiopsis,have the potential to have a serious impact on banana production.Existing studies are insufficient to provide a...Banana is a significant crop,and three banana leaf diseases,including Sigatoka,Cordana and Pestalotiopsis,have the potential to have a serious impact on banana production.Existing studies are insufficient to provide a reliable method for accurately identifying banana leaf diseases.Therefore,this paper proposes a novel method to identify banana leaf diseases.First,a new algorithm called K-scale VisuShrink algorithm(KVA)is proposed to denoise banana leaf images.The proposed algorithm introduces a new decomposition scale K based on the semi-soft and middle course thresholds,the ideal threshold solution is obtained and substituted with the newly established threshold function to obtain a less noisy banana leaf image.Then,this paper proposes a novel network for image identification called Ghost ResNeSt-Attention RReLU-Swish Net(GR-ARNet)based on Resnet50.In this,the Ghost Module is implemented to improve the network's effectiveness in extracting deep feature information on banana leaf diseases and the identification speed;the ResNeSt Module adjusts the weight of each channel,increasing the ability of banana disease feature extraction and effectively reducing the error rate of similar disease identification;the model's computational speed is increased using the hybrid activation function of RReLU and Swish.Our model achieves an average accuracy of 96.98%and a precision of 89.31%applied to 13,021 images,demonstrating that the proposed method can effectively identify banana leaf diseases.展开更多
Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry. However, apple leaf diseases do not differ significantly from ima...Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry. However, apple leaf diseases do not differ significantly from image texture and structural information. The difficulties in disease feature extraction in complex backgrounds slow the related research progress. To address the problems, this paper proposes an improved multi-scale inverse bottleneck residual network model based on a triplet parallel attention mechanism, which is built upon ResNet-50, while improving and combining the inception module and ResNext inverse bottleneck blocks, to recognize seven types of apple leaf(including six diseases of alternaria leaf spot, brown spot, grey spot, mosaic, rust, scab, and one healthy). First, the 3×3 convolutions in some of the residual modules are replaced by multi-scale residual convolutions, the convolution kernels of different sizes contained in each branch of the multi-scale convolution are applied to extract feature maps of different sizes, and the outputs of these branches are multi-scale fused by summing to enrich the output features of the images. Second, the global layer-wise dynamic coordinated inverse bottleneck structure is used to reduce the network feature loss. The inverse bottleneck structure makes the image information less lossy when transforming from different dimensional feature spaces. The fusion of multi-scale and layer-wise dynamic coordinated inverse bottlenecks makes the model effectively balances computational efficiency and feature representation capability, and more robust with a combination of horizontal and vertical features in the fine identification of apple leaf diseases. Finally, after each improved module, a triplet parallel attention module is integrated with cross-dimensional interactions among channels through rotations and residual transformations, which improves the parallel search efficiency of important features and the recognition rate of the network with relatively small computational costs while the dimensional dependencies are improved. To verify the validity of the model in this paper, we uniformly enhance apple leaf disease images screened from the public data sets of Plant Village, Baidu Flying Paddle, and the Internet. The final processed image count is 14,000. The ablation study, pre-processing comparison, and method comparison are conducted on the processed datasets. The experimental results demonstrate that the proposed method reaches 98.73% accuracy on the adopted datasets, which is 1.82% higher than the classical ResNet-50 model, and 0.29% better than the apple leaf disease datasets before preprocessing. It also achieves competitive results in apple leaf disease identification compared to some state-ofthe-art methods.展开更多
[Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed...[Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed to amplify the phytoplasma 16S rDNA of C.rutidosperma samples,followed by sequence analysis.Concurrently,this study examined C.rutidosperma in YLD field,collecting symptomatic leaves for phytoplasma detection.[Results]The 16S rDNA sequence of the C.rutidosperma witches'-broom phytoplasma was found to be identical to that of the HNWC5 strain associated with areca palm yellows phytoplasma,leading to the identification of this phytoplasma as belonging to the 16SrII-A subgroup.Field investigations revealed a higher incidence of C.rutidosperma in areca palm fields,with symptoms of leaf yellows observed in six of these fields.Quantitative PCR(qPCR)analysis confirmed the presence of phytoplasma infection in these instances.[Conclusions]Through the analysis of geographical distribution,sequence alignment,and field occurrence data,a significant correlation has been identified between witches'broom disease and YLD.It is proposed that the former may act as an intermediate host for the areca palm yellows phytoplasma.展开更多
Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10...Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10 years of collaborative research,and achieved several innovative achievements.SCWL phytoplasmas newly recorded in China and the new subgroup of SCWL phytoplasmas(16SrXI-D)were discovered for the first time in Yunnan,and the whole genome analysis of the epidemic subgroup was completed.The main transmission source of SCWL pathogens has been identified as infected seed canes,and Tettigoniella viridis and Clovia conifer were newly discovered as vectors for virus transmission.The disease resistance of 25 main varieties was identified,and 10 control varieties were selected.The prevention and control strategy of"emphasizing early warning,strictly carrying out quarantine,blocking the vectors and controlling residual plants"was put forward,and a comprehensive prevention technique was established through integration of various techniques,and standardized technical regulations were formulated for demonstration application.The promotion and application of these achievements have realized scientific prevention and control of SCWL,effectively curbed the spread of SCWL,and ensured the safety of sugarcane producing areas in China,achieving great economic,social and ecological benefits and providing technical support for high-quality development,loss reduction and efficiency improvement of China's sugar industry.展开更多
The guava plant has achieved viable significance in subtropics and tropics owing to its flexibility to climatic environments,soil conditions and higher human consumption.It is cultivated in vast areas of Asian and Non...The guava plant has achieved viable significance in subtropics and tropics owing to its flexibility to climatic environments,soil conditions and higher human consumption.It is cultivated in vast areas of Asian and Non-Asian countries,including Pakistan.The guava plant is vulnerable to diseases,specifically the leaves and fruit,which result in massive crop and profitability losses.The existing plant leaf disease detection techniques can detect only one disease from a leaf.However,a single leaf may contain symptoms of multiple diseases.This study has proposed a hybrid deep learning-based framework for the real-time detection of multiple diseases from a single guava leaf in several steps.Firstly,Guava Infected Patches Modified MobileNetV2 and U-Net(GIP-MU-NET)has been proposed to segment the infected guava patches.The proposed model consists of modified MobileNetv2 as an encoder,and the U-Net model’s up-sampling layers are used as a decoder part.Secondly,the Guava Leaf SegmentationModel(GLSM)is proposed to segment the healthy and infected leaves.In the final step,the Guava Multiple Leaf Diseases Detection(GMLDD)model based on the YOLOv5 model detects various diseases from a guava leaf.Two self-collected datasets(the Guava Patches Dataset and the Guava Leaf Diseases Dataset)are used for training and validation.The proposed method detected the various defects,including five distinct classes,i.e.,anthracnose,insect attack,nutrition deficiency,wilt,and healthy.On average,the GIP-MU-Net model achieved 92.41%accuracy,the GLSM gained 83.40%accuracy,whereas the proposed GMLDD technique achieved 73.3%precision,73.1%recall,71.0%mAP@0.5 and 50.3 mAP@0.5:0.95 scores for all the aforesaid classes.展开更多
In the field of agricultural information,the identification and prediction of rice leaf disease have always been the focus of research,and deep learning(DL)technology is currently a hot research topic in the field of ...In the field of agricultural information,the identification and prediction of rice leaf disease have always been the focus of research,and deep learning(DL)technology is currently a hot research topic in the field of pattern recognition.The research and development of high-efficiency,highquality and low-cost automatic identification methods for rice diseases that can replace humans is an important means of dealing with the current situation from a technical perspective.This paper mainly focuses on the problem of huge parameters of the Convolutional Neural Network(CNN)model and proposes a recognitionmodel that combines amulti-scale convolution module with a neural network model based on Visual Geometry Group(VGG).The accuracy and loss of the training set and the test set are used to evaluate the performance of the model.The test accuracy of this model is 97.1%that has increased 5.87%over VGG.Furthermore,the memory requirement is 26.1M,only 1.6%of the VGG.Experiment results show that this model performs better in terms of accuracy,recognition speed and memory size.展开更多
Tomato leaf diseases significantly impact crop production,necessitating early detection for sustainable farming.Deep Learning(DL)has recently shown excellent results in identifying and classifying tomato leaf diseases...Tomato leaf diseases significantly impact crop production,necessitating early detection for sustainable farming.Deep Learning(DL)has recently shown excellent results in identifying and classifying tomato leaf diseases.However,current DL methods often require substantial computational resources,hindering their application on resource-constrained devices.We propose the Deep Tomato Detection Network(DTomatoDNet),a lightweight DL-based framework comprising 19 learnable layers for efficient tomato leaf disease classification to overcome this.The Convn kernels used in the proposed(DTomatoDNet)framework is 1×1,which reduces the number of parameters and helps in more detailed and descriptive feature extraction for classification.The proposed DTomatoDNet model is trained from scratch to determine the classification success rate.10,000 tomato leaf images(1000 images per class)from the publicly accessible dataset,covering one healthy category and nine disease categories,are utilized in training the proposed DTomatoDNet approach.More specifically,we classified tomato leaf images into Target Spot(TS),Early Blight(EB),Late Blight(LB),Bacterial Spot(BS),Leaf Mold(LM),Tomato Yellow Leaf Curl Virus(YLCV),Septoria Leaf Spot(SLS),Spider Mites(SM),Tomato Mosaic Virus(MV),and Tomato Healthy(H).The proposed DTomatoDNet approach obtains a classification accuracy of 99.34%,demonstrating excellent accuracy in differentiating between tomato diseases.The model could be used on mobile platforms because it is lightweight and designed with fewer layers.Tomato farmers can utilize the proposed DTomatoDNet methodology to detect disease more quickly and easily once it has been integrated into mobile platforms by developing a mobile application.展开更多
The South Indian mango industry is confronting severe threats due to various leaf diseases,which significantly impact the yield and quality of the crop.The management and prevention of these diseases depend mainly on ...The South Indian mango industry is confronting severe threats due to various leaf diseases,which significantly impact the yield and quality of the crop.The management and prevention of these diseases depend mainly on their early identification and accurate classification.The central objective of this research is to propose and examine the application of Deep Convolutional Neural Networks(CNNs)as a potential solution for the precise detection and categorization of diseases impacting the leaves of South Indian mango trees.Our study collected a rich dataset of leaf images representing different disease classes,including Anthracnose,Powdery Mildew,and Leaf Blight.To maintain image quality and consistency,pre-processing techniques were employed.We then used a customized deep CNN architecture to analyze the accuracy of South Indian mango leaf disease detection and classification.This proposed CNN model was trained and evaluated using our collected dataset.The customized deep CNN model demonstrated high performance in experiments,achieving an impressive 93.34%classification accuracy.This result outperformed traditional CNN algorithms,indicating the potential of customized deep CNN as a dependable tool for disease diagnosis.Our proposed model showed superior accuracy and computational efficiency performance compared to other basic CNN models.Our research underscores the practical benefits of customized deep CNNs for automated leaf disease detection and classification in South Indian mango trees.These findings support deep CNN as a valuable tool for real-time interventions and improving crop management practices,thereby mitigating the issues currently facing the South Indian mango industry.展开更多
[Objectives]This study was conducted to establish simple, efficient, stable, standardized and practical identification methods for sugarcane resistance to white leaf disease(SCWL), and promote the breeding for sugarca...[Objectives]This study was conducted to establish simple, efficient, stable, standardized and practical identification methods for sugarcane resistance to white leaf disease(SCWL), and promote the breeding for sugarcane resistance to SCWL. [Methods]The identification technology of sugarcane resistance to SCWL was systematically studied and explored from the aspects of sugarcane material treatment and planting, inoculation liquid preparation, inoculation method, disease investigation, grading standard formulation, etc., and two sets of simple, efficient, stable, standardized and practical accurate identification methods for sugarcane resistance to SCWL were created for the first time, namely, the seed cane coating inoculation method and the stem-cutting inoculation method at the growth stage. The seed cane coating inoculation method includes the steps of directly screening SCWL phytoplasma, extracting juice from cane and adding 10 times of sterile water to prepare an inoculation liquid, spraying seed cane on plastic film to keep moisture, planting the inoculated materials in barrels in an insect-proof greenhouse for cultivation, investigating the incidence rate 30 d after inoculation, and evaluating the disease resistance according to the 1-5 level standard. The method of stem-cutting inoculation includes the steps of directly screening sugarcane stems carrying SCWL phytoplasma and adding 10 times of sterile water to prepare an inoculation liquid, cultivating the identification materials in an insect-proof greenhouse, dropping 100 μl of the inoculation liquid into each root incision with a pipette gun at the age of 6 months, investigating the incidence rate 20 d after planting, and evaluating the disease resistance according to the 1-5 level standard. [Results] The two methods are similar to the natural transmission method. After inoculation, SCML occurred significantly, with high sensitivity and good reproducibility. The results of resistance identification were consistent with those of natural disease in the field. Through the two inoculation methods and field natural disease investigation, the resistance of 10 main cultivars to SCML was identified, which was true and reliable. [Conclusions] This study can provide standard varieties for identification of SCML resistance in the future.展开更多
In the introduction and propagation of red sandalwood (Pterocarpus santalinus), a serious leaf disease of its seedlings in winter and spring seasons was found, but the name of the disease and its pathogen species ha...In the introduction and propagation of red sandalwood (Pterocarpus santalinus), a serious leaf disease of its seedlings in winter and spring seasons was found, but the name of the disease and its pathogen species have not been reported. The pathogen isolated from infected leaves of 18-month-old seedlings was identi- fied as Colletotrichum gloeosporioides by morphological characteristics of colony and conidium, and analysis results of rDNA-intemal transcribed spacer sequence (ITS) of the strain. Pathogenicity test results further confirmed that C. gloeosporioides was the pathogen responsible for the infected leaves symptoms of red sandal- wood. However, the disease belongs to an atypical anthraenose. Control of the leaf diseases of red sandalwood seedlings was discussed.展开更多
In India’s economy, agriculture has been the most significantcontributor. Despite the fact that agriculture’s contribution is decreasing asthe world’s population grows, it continues to be the most important sourceo...In India’s economy, agriculture has been the most significantcontributor. Despite the fact that agriculture’s contribution is decreasing asthe world’s population grows, it continues to be the most important sourceof employment with a little margin of difference. As a result, there is apressing need to pick up the pace in order to achieve competitive, productive,diverse, and long-term agriculture. Plant disease misinterpretations can resultin the incorrect application of pesticides, causing crop harm. As a result,early detection of infections is critical as well as cost-effective for farmers.To diagnose the disease at an earlier stage, appropriate segmentation of thediseased component from the leaf in an accurate manner is critical. However,due to the existence of noise in the digitally captured image, as well asvariations in backdrop, shape, and brightness in sick photographs, effectiverecognition has become a difficult task. Leaf smut, Bacterial blight andBrown spot diseases are segmented and classified using diseased Apple (20),Cercospora (60), Rice (100), Grape (140), and wheat (180) leaf photos in thesuggested work. In addition, a superior segmentation technique for the ROIfrom sick leaves with living backdrop is presented here. Textural features of thesegmented ROI, such as 1st and 2nd order WPCA Features, are discoveredafter segmentation. This comprises 1st order textural features like kurtosis,skewness, mean and variance as well as 2nd procedure textural features likesmoothness, energy, correlation, homogeneity, contrast, and entropy. Finally,the segmented region of interest’s textural features is fed into four differentclassifiers, with the Enhanced Deep Convolutional Neural Network provingto be the most precise, with a 96.1% accuracy.展开更多
In the actual complex environment,the recognition accuracy of crop leaf disease is often not high.Inspired by the brain parallel interaction mechanism,a two-stream parallel interactive convolutional neural network(TSP...In the actual complex environment,the recognition accuracy of crop leaf disease is often not high.Inspired by the brain parallel interaction mechanism,a two-stream parallel interactive convolutional neural network(TSPI-CNN)is proposed to improve the recognition accuracy.TSPI-CNN includes a two-stream parallel network(TSP-Net)and a parallel interactive network(PI-Net).TSP-Net simulates the ventral and dorsal stream.PI-Net simulates the interaction between two pathways in the process of human brain visual information transmission.A large number of experiments shows that the proposed TSPI-CNN performs well on MK-D2,PlantVillage,Apple-3 leaf,and Cassava leaf datasets.Furthermore,the effect of numbers of interactions on the recognition performance of TSPI-CNN is discussed.The experimental results show that as the number of interactions increases,the recognition accuracy of the network also increases.Finally,the network is visualized to show the working mechanism of the network and provide enlightenment for future research.展开更多
Precision agriculture enables the recent technological advancements in farming sector to observe,measure,and analyze the requirements of individual fields and crops.The recent developments of computer vision and artif...Precision agriculture enables the recent technological advancements in farming sector to observe,measure,and analyze the requirements of individual fields and crops.The recent developments of computer vision and artificial intelligence(AI)techniques find a way for effective detection of plants,diseases,weeds,pests,etc.On the other hand,the detection of plant diseases,particularly apple leaf diseases using AI techniques can improve productivity and reduce crop loss.Besides,earlier and precise apple leaf disease detection can minimize the spread of the disease.Earlier works make use of traditional image processing techniques which cannot assure high detection rate on apple leaf diseases.With this motivation,this paper introduces a novel AI enabled apple leaf disease classification(AIE-ALDC)technique for precision agriculture.The proposed AIE-ALDC technique involves orientation based data augmentation and Gaussian filtering based noise removal processes.In addition,the AIE-ALDC technique includes a Capsule Network(CapsNet)based feature extractor to generate a helpful set of feature vectors.Moreover,water wave optimization(WWO)technique is employed as a hyperparameter optimizer of the CapsNet model.Finally,bidirectional long short term memory(BiLSTM)model is used as a classifier to determine the appropriate class labels of the apple leaf images.The design of AIE-ALDC technique incorporating theWWO based CapsNetmodel with BiLSTM classifier shows the novelty of the work.Awide range of experiments was performed to showcase the supremacy of the AIE-ALDC technique.The experimental results demonstrate the promising performance of the AIEALDC technique over the recent state of art methods.展开更多
Due to the high demand for mango and being the king of all fruits,it is the need of the hour to curb its diseases to fetch high returns.Automatic leaf disease segmentation and identification are still a challenge due ...Due to the high demand for mango and being the king of all fruits,it is the need of the hour to curb its diseases to fetch high returns.Automatic leaf disease segmentation and identification are still a challenge due to variations in symptoms.Accurate segmentation of the disease is the key prerequisite for any computer-aided system to recognize the diseases,i.e.,Anthracnose,apicalnecrosis,etc.,of a mango plant leaf.To solve this issue,we proposed a CNN based Fully-convolutional-network(FrCNnet)model for the segmentation of the diseased part of the mango leaf.The proposed FrCNnet directly learns the features of each pixel of the input data after applying some preprocessing techniques.We evaluated the proposed FrCNnet on the real-time dataset provided by the mango research institute,Multan,Pakistan.To evaluate the proposed model results,we compared the segmentation performance with the available state-of-the-art models,i.e.,Vgg16,Vgg-19,and Unet.Furthermore,the proposed model’s segmentation accuracy is 99.2%with a false negative rate(FNR)of 0.8%,which is much higher than the other models.We have concluded that by using a FrCNnet,the input image could learn better features that are more prominent and much specific,resulting in an improved and better segmentation performance and diseases’identification.Accordingly,an automated approach helps pathologists and mango growers detect and identify those diseases.展开更多
To solve the problem of mistake recognition among rice diseases, automatic recognition methods based on BP(back propagation) neural network were studied in this paper for blast, sheath blight and bacterial blight. Cho...To solve the problem of mistake recognition among rice diseases, automatic recognition methods based on BP(back propagation) neural network were studied in this paper for blast, sheath blight and bacterial blight. Chose mobile terminal equipment as image collecting tool and built database of rice leaf images with diseases under threshold segmentation method. Characteristic parameters were extracted from color, shape and texture. Furthermore, parameters were optimized using the single-factor variance analysis and the effects of BP neural network model. The optimization would simplify BP neural network model without reducing the recognition accuracy. The finally model could successfully recognize 98%, 96% and 98% of rice blast, sheath blight and white leaf blight, respectively.展开更多
A simple and highly sensitive analysis by electrochemical voltammetry has been developed for diagnosis of the most destructive crop disease in Thailand known as sugarcane white leaf (SCWL). Determination of the corres...A simple and highly sensitive analysis by electrochemical voltammetry has been developed for diagnosis of the most destructive crop disease in Thailand known as sugarcane white leaf (SCWL). Determination of the corresponding DNA interaction has been obtained from the voltammetric signals of electroactive redox methylene blue (MB) by means of cyclic and differential pulse voltammetry. In this study, a chitosan-modified glassy carbon electrode (GCE) was created by self-assembly to produce electrostatic platform for effective immobilization of the DNA. Fabrication of SCWL-DNA hybridization detection system was performed by immobilizing the ssDNA probe as a specific sensor onto chitosan-modified GCE. Hybridization of complementary DNA from the real samples could then be detected by its respective MB signal. This fabricated DNA probe sensor was shown to be capable for discriminative identification among the DNAs from SCWL plants, mosaic virus infected sugarcane and healthy sugarcane plants. Relationship between the specific hybridization signal and DNA target concentration was also observed under optimal condition. The detection limit of 4.709 ng/μl with the regression coefficient (R2) of 0.998 and overall RSD of 2.44% were obtained by response curve fit analysis. The actual SCWL-ssDNA immobilization and hybridizing event were subsequently confirmed by an observation under atomic force microscope. Thus these experiments demonstrate the first successful and effective DNA based voltammetric electrochemical determination for a verification of the specific pathogenic infection within plants from the real epidemic field.展开更多
Rice stands as a crucial staple food globally,with its enduring sustainability hinging on the prompt detection of rice leaf diseases.Hence,efficiently detecting diseases when they have already occurred holds paramount...Rice stands as a crucial staple food globally,with its enduring sustainability hinging on the prompt detection of rice leaf diseases.Hence,efficiently detecting diseases when they have already occurred holds paramount importance for solving the cost of manual visual identification and chemical testing.In the recent past,the identification of leaf pathologies in crops predominantly relies on manual methods using specialized equipment,which proves to be time-consuming and inefficient.This study offers a remedy by harnessing Deep Learning(DL)and transfer learning techniques to accurately identify and classify rice leaf diseases.A comprehensive dataset comprising 5932 self-generated images of rice leaves was assembled along with the benchmark datasets,categorized into 9 classes irrespective of the extent of disease spread across the leaves.These classes encompass diverse states including healthy leaves,mild and severe blight,mild and severe tungro,mild and severe blast,as well as mild and severe brown spot.Following meticulous manual labelling and dataset segmentation,which was validated by horticulture experts,data augmentation strategies were implemented to amplify the number of images.The datasets were subjected to evaluation using the proposed tailored Convolutional Neural Networks models.Their performance are scrutinized in conjunction with alternative transfer learning approaches like VGG16,Xception,ResNet50,DenseNet121,Inception ResnetV2,and Inception V3.The effectiveness of the proposed custom VGG16 model was gauged by its capacity to generalize to unseen images,yielding an exceptional accuracy of 99.94%,surpassing the benchmarks set by existing state-of-the-art models.Further,the layer wise feature extraction is also visualized as the interpretable AI.展开更多
As an important rice disease, rice bacterial leaf blight (RBLB, caused by the bacterium Xanthomonas oryzae pv.oryzae), has become widespread in east China in recent years. Significant losses in rice yield occurred as ...As an important rice disease, rice bacterial leaf blight (RBLB, caused by the bacterium Xanthomonas oryzae pv.oryzae), has become widespread in east China in recent years. Significant losses in rice yield occurred as a result ofthe disease’s epidemic, making it imperative to monitor RBLB at a large scale. With the development of remotesensing technology, the broad-band sensors equipped with red-edge channels over multiple spatial resolutionsoffer numerous available data for large-scale monitoring of rice diseases. However, RBLB is characterized by rapiddispersal under suitable conditions, making it difficult to track the disease at a regional scale with a single sensorin practice. Therefore, it is necessary to identify or construct features that are effective across different sensors formonitoring RBLB. To achieve this goal, the spectral response of RBLB was first analyzed based on the canopyhyperspectral data. Using the relative spectral response (RSR) functions of four representative satellite or UAVsensors (i.e., Sentinel-2, GF-6, Planet, and Rededge-M) and the hyperspectral data, the corresponding broad-bandspectral data was simulated. According to a thorough band combination and sensitivity analysis, two novel spectralindices for monitoring RBLB that can be effective across multiple sensors (i.e., RBBRI and RBBDI) weredeveloped. An optimal feature set that includes the two novel indices and a classical vegetation index was formed.The capability of such a feature set in monitoring RBLB was assessed via FLDA and SVM algorithms. The resultdemonstrated that both constructed novel indices exhibited high sensitivity to the disease across multiple sensors.Meanwhile, the feature set yielded an overall accuracy above 90% for all sensors, which indicates its cross-sensorgenerality in monitoring RBLB. The outcome of this research permits disease monitoring with different remotesensing data over a large scale.展开更多
Leaf disease recognition using image processing and deep learning techniques is currently a vibrant research area.Most studies have focused on recognizing diseases from images of whole leaves.This approach limits the ...Leaf disease recognition using image processing and deep learning techniques is currently a vibrant research area.Most studies have focused on recognizing diseases from images of whole leaves.This approach limits the resulting models’ability to estimate leaf disease severity or identify multiple anomalies occurring on the same leaf.Recent studies have demonstrated that classifying leaf diseases based on individual lesions greatly enhances disease recognition accuracy.In those studies,however,the lesions were laboriously cropped by hand.This study proposes a semi-automatic algorithm that facilitates the fast and efficient preparation of datasets of individual lesions and leaf image pixel maps to overcome this problem.These datasets were then used to train and test lesion classifier and semantic segmentation Convolutional Neural Network(CNN)models,respectively.We report that GoogLeNet’s disease recognition accuracy improved by more than 15%when diseases were recognized from lesion images compared to when disease recognition was done using images of whole leaves.A CNN model which performs semantic segmentation of both the leaf and lesions in one pass is also proposed in this paper.The proposed KijaniNet model achieved state-of-the-art segmentation performance in terms of mean Intersection over Union(mIoU)score of 0.8448 and 0.6257 for the leaf and lesion pixel classes,respectively.In terms of mean boundary F1 score,the KijaniNet model attained 0.8241 and 0.7855 for the two pixel classes,respectively.Lastly,a fully automatic algorithm for leaf disease recognition from individual lesions is proposed.The algorithm employs the semantic segmentation network cascaded to a GoogLeNet classifier for lesion-wise disease recognition.The proposed fully automatic algorithm outperforms competing methods in terms of its superior segmentation and classification performance despite being trained on a small dataset.展开更多
文摘Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wavelet,feature-fused,pre-trained deep learning model for detecting olive leaf diseases.The proposed model combines wavelet transforms with pre-trained deep-learning models to extract discriminative features from olive leaf images.The model has four main phases:preprocessing using data augmentation,three-level wavelet transformation,learning using pre-trained deep learning models,and a fused deep learning model.In the preprocessing phase,the image dataset is augmented using techniques such as resizing,rescaling,flipping,rotation,zooming,and contrasting.In wavelet transformation,the augmented images are decomposed into three frequency levels.Three pre-trained deep learning models,EfficientNet-B7,DenseNet-201,and ResNet-152-V2,are used in the learning phase.The models were trained using the approximate images of the third-level sub-band of the wavelet transform.In the fused phase,the fused model consists of a merge layer,three dense layers,and two dropout layers.The proposed model was evaluated using a dataset of images of healthy and infected olive leaves.It achieved an accuracy of 99.72%in the diagnosis of olive leaf diseases,which exceeds the accuracy of other methods reported in the literature.This finding suggests that our proposed method is a promising tool for the early detection of olive leaf diseases.
基金supported by the Changsha Municipal Natural Science Foundation,China(kq2014160)in part by the Key Projects of Department of Education of Hunan Province,China(21A0179)+1 种基金the Hunan Key Laboratory of Intelligent Logistics Technology,China(2019TP1015)the National Natural Science Foundation of China(61902436)。
文摘Banana is a significant crop,and three banana leaf diseases,including Sigatoka,Cordana and Pestalotiopsis,have the potential to have a serious impact on banana production.Existing studies are insufficient to provide a reliable method for accurately identifying banana leaf diseases.Therefore,this paper proposes a novel method to identify banana leaf diseases.First,a new algorithm called K-scale VisuShrink algorithm(KVA)is proposed to denoise banana leaf images.The proposed algorithm introduces a new decomposition scale K based on the semi-soft and middle course thresholds,the ideal threshold solution is obtained and substituted with the newly established threshold function to obtain a less noisy banana leaf image.Then,this paper proposes a novel network for image identification called Ghost ResNeSt-Attention RReLU-Swish Net(GR-ARNet)based on Resnet50.In this,the Ghost Module is implemented to improve the network's effectiveness in extracting deep feature information on banana leaf diseases and the identification speed;the ResNeSt Module adjusts the weight of each channel,increasing the ability of banana disease feature extraction and effectively reducing the error rate of similar disease identification;the model's computational speed is increased using the hybrid activation function of RReLU and Swish.Our model achieves an average accuracy of 96.98%and a precision of 89.31%applied to 13,021 images,demonstrating that the proposed method can effectively identify banana leaf diseases.
基金supported in part by the General Program Hunan Provincial Natural Science Foundation of 2022,China(2022JJ31022)the Undergraduate Education Reform Project of Hunan Province,China(HNJG-20210532)the National Natural Science Foundation of China(62276276)。
文摘Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry. However, apple leaf diseases do not differ significantly from image texture and structural information. The difficulties in disease feature extraction in complex backgrounds slow the related research progress. To address the problems, this paper proposes an improved multi-scale inverse bottleneck residual network model based on a triplet parallel attention mechanism, which is built upon ResNet-50, while improving and combining the inception module and ResNext inverse bottleneck blocks, to recognize seven types of apple leaf(including six diseases of alternaria leaf spot, brown spot, grey spot, mosaic, rust, scab, and one healthy). First, the 3×3 convolutions in some of the residual modules are replaced by multi-scale residual convolutions, the convolution kernels of different sizes contained in each branch of the multi-scale convolution are applied to extract feature maps of different sizes, and the outputs of these branches are multi-scale fused by summing to enrich the output features of the images. Second, the global layer-wise dynamic coordinated inverse bottleneck structure is used to reduce the network feature loss. The inverse bottleneck structure makes the image information less lossy when transforming from different dimensional feature spaces. The fusion of multi-scale and layer-wise dynamic coordinated inverse bottlenecks makes the model effectively balances computational efficiency and feature representation capability, and more robust with a combination of horizontal and vertical features in the fine identification of apple leaf diseases. Finally, after each improved module, a triplet parallel attention module is integrated with cross-dimensional interactions among channels through rotations and residual transformations, which improves the parallel search efficiency of important features and the recognition rate of the network with relatively small computational costs while the dimensional dependencies are improved. To verify the validity of the model in this paper, we uniformly enhance apple leaf disease images screened from the public data sets of Plant Village, Baidu Flying Paddle, and the Internet. The final processed image count is 14,000. The ablation study, pre-processing comparison, and method comparison are conducted on the processed datasets. The experimental results demonstrate that the proposed method reaches 98.73% accuracy on the adopted datasets, which is 1.82% higher than the classical ResNet-50 model, and 0.29% better than the apple leaf disease datasets before preprocessing. It also achieves competitive results in apple leaf disease identification compared to some state-ofthe-art methods.
基金Supported by Innovation Platform for Academicians of Hainan Province of China(YSPTZX202151,YSPTZX202138)Hainan Provincial Natural Science Foundation of China(321QN345).
文摘[Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed to amplify the phytoplasma 16S rDNA of C.rutidosperma samples,followed by sequence analysis.Concurrently,this study examined C.rutidosperma in YLD field,collecting symptomatic leaves for phytoplasma detection.[Results]The 16S rDNA sequence of the C.rutidosperma witches'-broom phytoplasma was found to be identical to that of the HNWC5 strain associated with areca palm yellows phytoplasma,leading to the identification of this phytoplasma as belonging to the 16SrII-A subgroup.Field investigations revealed a higher incidence of C.rutidosperma in areca palm fields,with symptoms of leaf yellows observed in six of these fields.Quantitative PCR(qPCR)analysis confirmed the presence of phytoplasma infection in these instances.[Conclusions]Through the analysis of geographical distribution,sequence alignment,and field occurrence data,a significant correlation has been identified between witches'broom disease and YLD.It is proposed that the former may act as an intermediate host for the areca palm yellows phytoplasma.
基金Supported by National Natural Science Foundation of China(31760504)China Agriculture Research System of MOF and MARA(CARS-17)Special Fund for the Construction of Modern Agricultural Industrial Technology System in Yunnan Province.
文摘Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10 years of collaborative research,and achieved several innovative achievements.SCWL phytoplasmas newly recorded in China and the new subgroup of SCWL phytoplasmas(16SrXI-D)were discovered for the first time in Yunnan,and the whole genome analysis of the epidemic subgroup was completed.The main transmission source of SCWL pathogens has been identified as infected seed canes,and Tettigoniella viridis and Clovia conifer were newly discovered as vectors for virus transmission.The disease resistance of 25 main varieties was identified,and 10 control varieties were selected.The prevention and control strategy of"emphasizing early warning,strictly carrying out quarantine,blocking the vectors and controlling residual plants"was put forward,and a comprehensive prevention technique was established through integration of various techniques,and standardized technical regulations were formulated for demonstration application.The promotion and application of these achievements have realized scientific prevention and control of SCWL,effectively curbed the spread of SCWL,and ensured the safety of sugarcane producing areas in China,achieving great economic,social and ecological benefits and providing technical support for high-quality development,loss reduction and efficiency improvement of China's sugar industry.
基金financially supported by the Deanship of Scientific Research,Qassim University,Saudi Arabia for funding the publication of this project.
文摘The guava plant has achieved viable significance in subtropics and tropics owing to its flexibility to climatic environments,soil conditions and higher human consumption.It is cultivated in vast areas of Asian and Non-Asian countries,including Pakistan.The guava plant is vulnerable to diseases,specifically the leaves and fruit,which result in massive crop and profitability losses.The existing plant leaf disease detection techniques can detect only one disease from a leaf.However,a single leaf may contain symptoms of multiple diseases.This study has proposed a hybrid deep learning-based framework for the real-time detection of multiple diseases from a single guava leaf in several steps.Firstly,Guava Infected Patches Modified MobileNetV2 and U-Net(GIP-MU-NET)has been proposed to segment the infected guava patches.The proposed model consists of modified MobileNetv2 as an encoder,and the U-Net model’s up-sampling layers are used as a decoder part.Secondly,the Guava Leaf SegmentationModel(GLSM)is proposed to segment the healthy and infected leaves.In the final step,the Guava Multiple Leaf Diseases Detection(GMLDD)model based on the YOLOv5 model detects various diseases from a guava leaf.Two self-collected datasets(the Guava Patches Dataset and the Guava Leaf Diseases Dataset)are used for training and validation.The proposed method detected the various defects,including five distinct classes,i.e.,anthracnose,insect attack,nutrition deficiency,wilt,and healthy.On average,the GIP-MU-Net model achieved 92.41%accuracy,the GLSM gained 83.40%accuracy,whereas the proposed GMLDD technique achieved 73.3%precision,73.1%recall,71.0%mAP@0.5 and 50.3 mAP@0.5:0.95 scores for all the aforesaid classes.
基金supported by National key research and development program sub-topics[2018YFF0213606-03(Mu Y.,Hu T.L.,Gong H.,Li S.J.and Sun Y.H.)http://www.most.gov.cn]Jilin Province Science and Technology Development Plan focuses on research and development projects[20200402006NC(Mu Y.,Hu T.L.,Gong H.and Li S.J.)http://kjt.jl.gov.cn]+1 种基金Science and technology support project for key industries in southern Xinjiang[2018DB001(Gong H.,and Li S.J.)http://kjj.xjbt.gov.cn]Key technology R&D project of Changchun Science and Technology Bureau of Jilin Province[21ZGN29(Mu Y.,Bao H.P.,Wang X.B.)http://kjj.changchun.gov.cn].
文摘In the field of agricultural information,the identification and prediction of rice leaf disease have always been the focus of research,and deep learning(DL)technology is currently a hot research topic in the field of pattern recognition.The research and development of high-efficiency,highquality and low-cost automatic identification methods for rice diseases that can replace humans is an important means of dealing with the current situation from a technical perspective.This paper mainly focuses on the problem of huge parameters of the Convolutional Neural Network(CNN)model and proposes a recognitionmodel that combines amulti-scale convolution module with a neural network model based on Visual Geometry Group(VGG).The accuracy and loss of the training set and the test set are used to evaluate the performance of the model.The test accuracy of this model is 97.1%that has increased 5.87%over VGG.Furthermore,the memory requirement is 26.1M,only 1.6%of the VGG.Experiment results show that this model performs better in terms of accuracy,recognition speed and memory size.
基金thankful to the Deanship of Scientific Research at Najran University for funding this work under the Research Group Funding Program Grant Code(NU/RG/SERC/12/3)funded by Princess Nourah bint Abdulrahman University Researchers.Supporting Project Number(PNURSP2023R409),Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Tomato leaf diseases significantly impact crop production,necessitating early detection for sustainable farming.Deep Learning(DL)has recently shown excellent results in identifying and classifying tomato leaf diseases.However,current DL methods often require substantial computational resources,hindering their application on resource-constrained devices.We propose the Deep Tomato Detection Network(DTomatoDNet),a lightweight DL-based framework comprising 19 learnable layers for efficient tomato leaf disease classification to overcome this.The Convn kernels used in the proposed(DTomatoDNet)framework is 1×1,which reduces the number of parameters and helps in more detailed and descriptive feature extraction for classification.The proposed DTomatoDNet model is trained from scratch to determine the classification success rate.10,000 tomato leaf images(1000 images per class)from the publicly accessible dataset,covering one healthy category and nine disease categories,are utilized in training the proposed DTomatoDNet approach.More specifically,we classified tomato leaf images into Target Spot(TS),Early Blight(EB),Late Blight(LB),Bacterial Spot(BS),Leaf Mold(LM),Tomato Yellow Leaf Curl Virus(YLCV),Septoria Leaf Spot(SLS),Spider Mites(SM),Tomato Mosaic Virus(MV),and Tomato Healthy(H).The proposed DTomatoDNet approach obtains a classification accuracy of 99.34%,demonstrating excellent accuracy in differentiating between tomato diseases.The model could be used on mobile platforms because it is lightweight and designed with fewer layers.Tomato farmers can utilize the proposed DTomatoDNet methodology to detect disease more quickly and easily once it has been integrated into mobile platforms by developing a mobile application.
文摘The South Indian mango industry is confronting severe threats due to various leaf diseases,which significantly impact the yield and quality of the crop.The management and prevention of these diseases depend mainly on their early identification and accurate classification.The central objective of this research is to propose and examine the application of Deep Convolutional Neural Networks(CNNs)as a potential solution for the precise detection and categorization of diseases impacting the leaves of South Indian mango trees.Our study collected a rich dataset of leaf images representing different disease classes,including Anthracnose,Powdery Mildew,and Leaf Blight.To maintain image quality and consistency,pre-processing techniques were employed.We then used a customized deep CNN architecture to analyze the accuracy of South Indian mango leaf disease detection and classification.This proposed CNN model was trained and evaluated using our collected dataset.The customized deep CNN model demonstrated high performance in experiments,achieving an impressive 93.34%classification accuracy.This result outperformed traditional CNN algorithms,indicating the potential of customized deep CNN as a dependable tool for disease diagnosis.Our proposed model showed superior accuracy and computational efficiency performance compared to other basic CNN models.Our research underscores the practical benefits of customized deep CNNs for automated leaf disease detection and classification in South Indian mango trees.These findings support deep CNN as a valuable tool for real-time interventions and improving crop management practices,thereby mitigating the issues currently facing the South Indian mango industry.
基金Supported by National Natural Science Foundation of China (31760504)China Agriculture Research System of MOF and MARA(CARS-170303)+1 种基金Yunling Industry and Technology Leading Talent Training Program (2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province。
文摘[Objectives]This study was conducted to establish simple, efficient, stable, standardized and practical identification methods for sugarcane resistance to white leaf disease(SCWL), and promote the breeding for sugarcane resistance to SCWL. [Methods]The identification technology of sugarcane resistance to SCWL was systematically studied and explored from the aspects of sugarcane material treatment and planting, inoculation liquid preparation, inoculation method, disease investigation, grading standard formulation, etc., and two sets of simple, efficient, stable, standardized and practical accurate identification methods for sugarcane resistance to SCWL were created for the first time, namely, the seed cane coating inoculation method and the stem-cutting inoculation method at the growth stage. The seed cane coating inoculation method includes the steps of directly screening SCWL phytoplasma, extracting juice from cane and adding 10 times of sterile water to prepare an inoculation liquid, spraying seed cane on plastic film to keep moisture, planting the inoculated materials in barrels in an insect-proof greenhouse for cultivation, investigating the incidence rate 30 d after inoculation, and evaluating the disease resistance according to the 1-5 level standard. The method of stem-cutting inoculation includes the steps of directly screening sugarcane stems carrying SCWL phytoplasma and adding 10 times of sterile water to prepare an inoculation liquid, cultivating the identification materials in an insect-proof greenhouse, dropping 100 μl of the inoculation liquid into each root incision with a pipette gun at the age of 6 months, investigating the incidence rate 20 d after planting, and evaluating the disease resistance according to the 1-5 level standard. [Results] The two methods are similar to the natural transmission method. After inoculation, SCML occurred significantly, with high sensitivity and good reproducibility. The results of resistance identification were consistent with those of natural disease in the field. Through the two inoculation methods and field natural disease investigation, the resistance of 10 main cultivars to SCML was identified, which was true and reliable. [Conclusions] This study can provide standard varieties for identification of SCML resistance in the future.
基金Supported by Natural Science Foundation of China(31270674)Innovative High School Key Research Platform of Zhaoqing University(CQ201607)
文摘In the introduction and propagation of red sandalwood (Pterocarpus santalinus), a serious leaf disease of its seedlings in winter and spring seasons was found, but the name of the disease and its pathogen species have not been reported. The pathogen isolated from infected leaves of 18-month-old seedlings was identi- fied as Colletotrichum gloeosporioides by morphological characteristics of colony and conidium, and analysis results of rDNA-intemal transcribed spacer sequence (ITS) of the strain. Pathogenicity test results further confirmed that C. gloeosporioides was the pathogen responsible for the infected leaves symptoms of red sandal- wood. However, the disease belongs to an atypical anthraenose. Control of the leaf diseases of red sandalwood seedlings was discussed.
文摘In India’s economy, agriculture has been the most significantcontributor. Despite the fact that agriculture’s contribution is decreasing asthe world’s population grows, it continues to be the most important sourceof employment with a little margin of difference. As a result, there is apressing need to pick up the pace in order to achieve competitive, productive,diverse, and long-term agriculture. Plant disease misinterpretations can resultin the incorrect application of pesticides, causing crop harm. As a result,early detection of infections is critical as well as cost-effective for farmers.To diagnose the disease at an earlier stage, appropriate segmentation of thediseased component from the leaf in an accurate manner is critical. However,due to the existence of noise in the digitally captured image, as well asvariations in backdrop, shape, and brightness in sick photographs, effectiverecognition has become a difficult task. Leaf smut, Bacterial blight andBrown spot diseases are segmented and classified using diseased Apple (20),Cercospora (60), Rice (100), Grape (140), and wheat (180) leaf photos in thesuggested work. In addition, a superior segmentation technique for the ROIfrom sick leaves with living backdrop is presented here. Textural features of thesegmented ROI, such as 1st and 2nd order WPCA Features, are discoveredafter segmentation. This comprises 1st order textural features like kurtosis,skewness, mean and variance as well as 2nd procedure textural features likesmoothness, energy, correlation, homogeneity, contrast, and entropy. Finally,the segmented region of interest’s textural features is fed into four differentclassifiers, with the Enhanced Deep Convolutional Neural Network provingto be the most precise, with a 96.1% accuracy.
基金National Natural Science Foundation of China(Nos.61806051 and 61903078)Fundamental Research Funds for the Central Universities,China(Nos.2232021A-10 and 2232021D-32)Natural Science Foundation of Shanghai,China(No.20ZR1400400)。
文摘In the actual complex environment,the recognition accuracy of crop leaf disease is often not high.Inspired by the brain parallel interaction mechanism,a two-stream parallel interactive convolutional neural network(TSPI-CNN)is proposed to improve the recognition accuracy.TSPI-CNN includes a two-stream parallel network(TSP-Net)and a parallel interactive network(PI-Net).TSP-Net simulates the ventral and dorsal stream.PI-Net simulates the interaction between two pathways in the process of human brain visual information transmission.A large number of experiments shows that the proposed TSPI-CNN performs well on MK-D2,PlantVillage,Apple-3 leaf,and Cassava leaf datasets.Furthermore,the effect of numbers of interactions on the recognition performance of TSPI-CNN is discussed.The experimental results show that as the number of interactions increases,the recognition accuracy of the network also increases.Finally,the network is visualized to show the working mechanism of the network and provide enlightenment for future research.
基金The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for funding this work under Grant Number(RGP2/209/42),www.kku.e du.sa.This research was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University through the Fast-Track Path of Research Funding Program.
文摘Precision agriculture enables the recent technological advancements in farming sector to observe,measure,and analyze the requirements of individual fields and crops.The recent developments of computer vision and artificial intelligence(AI)techniques find a way for effective detection of plants,diseases,weeds,pests,etc.On the other hand,the detection of plant diseases,particularly apple leaf diseases using AI techniques can improve productivity and reduce crop loss.Besides,earlier and precise apple leaf disease detection can minimize the spread of the disease.Earlier works make use of traditional image processing techniques which cannot assure high detection rate on apple leaf diseases.With this motivation,this paper introduces a novel AI enabled apple leaf disease classification(AIE-ALDC)technique for precision agriculture.The proposed AIE-ALDC technique involves orientation based data augmentation and Gaussian filtering based noise removal processes.In addition,the AIE-ALDC technique includes a Capsule Network(CapsNet)based feature extractor to generate a helpful set of feature vectors.Moreover,water wave optimization(WWO)technique is employed as a hyperparameter optimizer of the CapsNet model.Finally,bidirectional long short term memory(BiLSTM)model is used as a classifier to determine the appropriate class labels of the apple leaf images.The design of AIE-ALDC technique incorporating theWWO based CapsNetmodel with BiLSTM classifier shows the novelty of the work.Awide range of experiments was performed to showcase the supremacy of the AIE-ALDC technique.The experimental results demonstrate the promising performance of the AIEALDC technique over the recent state of art methods.
文摘Due to the high demand for mango and being the king of all fruits,it is the need of the hour to curb its diseases to fetch high returns.Automatic leaf disease segmentation and identification are still a challenge due to variations in symptoms.Accurate segmentation of the disease is the key prerequisite for any computer-aided system to recognize the diseases,i.e.,Anthracnose,apicalnecrosis,etc.,of a mango plant leaf.To solve this issue,we proposed a CNN based Fully-convolutional-network(FrCNnet)model for the segmentation of the diseased part of the mango leaf.The proposed FrCNnet directly learns the features of each pixel of the input data after applying some preprocessing techniques.We evaluated the proposed FrCNnet on the real-time dataset provided by the mango research institute,Multan,Pakistan.To evaluate the proposed model results,we compared the segmentation performance with the available state-of-the-art models,i.e.,Vgg16,Vgg-19,and Unet.Furthermore,the proposed model’s segmentation accuracy is 99.2%with a false negative rate(FNR)of 0.8%,which is much higher than the other models.We have concluded that by using a FrCNnet,the input image could learn better features that are more prominent and much specific,resulting in an improved and better segmentation performance and diseases’identification.Accordingly,an automated approach helps pathologists and mango growers detect and identify those diseases.
基金Supported by Quality and Brand Construction of"Internet+County Characteristic Agricultural Products"(ZY17C06)
文摘To solve the problem of mistake recognition among rice diseases, automatic recognition methods based on BP(back propagation) neural network were studied in this paper for blast, sheath blight and bacterial blight. Chose mobile terminal equipment as image collecting tool and built database of rice leaf images with diseases under threshold segmentation method. Characteristic parameters were extracted from color, shape and texture. Furthermore, parameters were optimized using the single-factor variance analysis and the effects of BP neural network model. The optimization would simplify BP neural network model without reducing the recognition accuracy. The finally model could successfully recognize 98%, 96% and 98% of rice blast, sheath blight and white leaf blight, respectively.
文摘A simple and highly sensitive analysis by electrochemical voltammetry has been developed for diagnosis of the most destructive crop disease in Thailand known as sugarcane white leaf (SCWL). Determination of the corresponding DNA interaction has been obtained from the voltammetric signals of electroactive redox methylene blue (MB) by means of cyclic and differential pulse voltammetry. In this study, a chitosan-modified glassy carbon electrode (GCE) was created by self-assembly to produce electrostatic platform for effective immobilization of the DNA. Fabrication of SCWL-DNA hybridization detection system was performed by immobilizing the ssDNA probe as a specific sensor onto chitosan-modified GCE. Hybridization of complementary DNA from the real samples could then be detected by its respective MB signal. This fabricated DNA probe sensor was shown to be capable for discriminative identification among the DNAs from SCWL plants, mosaic virus infected sugarcane and healthy sugarcane plants. Relationship between the specific hybridization signal and DNA target concentration was also observed under optimal condition. The detection limit of 4.709 ng/μl with the regression coefficient (R2) of 0.998 and overall RSD of 2.44% were obtained by response curve fit analysis. The actual SCWL-ssDNA immobilization and hybridizing event were subsequently confirmed by an observation under atomic force microscope. Thus these experiments demonstrate the first successful and effective DNA based voltammetric electrochemical determination for a verification of the specific pathogenic infection within plants from the real epidemic field.
文摘Rice stands as a crucial staple food globally,with its enduring sustainability hinging on the prompt detection of rice leaf diseases.Hence,efficiently detecting diseases when they have already occurred holds paramount importance for solving the cost of manual visual identification and chemical testing.In the recent past,the identification of leaf pathologies in crops predominantly relies on manual methods using specialized equipment,which proves to be time-consuming and inefficient.This study offers a remedy by harnessing Deep Learning(DL)and transfer learning techniques to accurately identify and classify rice leaf diseases.A comprehensive dataset comprising 5932 self-generated images of rice leaves was assembled along with the benchmark datasets,categorized into 9 classes irrespective of the extent of disease spread across the leaves.These classes encompass diverse states including healthy leaves,mild and severe blight,mild and severe tungro,mild and severe blast,as well as mild and severe brown spot.Following meticulous manual labelling and dataset segmentation,which was validated by horticulture experts,data augmentation strategies were implemented to amplify the number of images.The datasets were subjected to evaluation using the proposed tailored Convolutional Neural Networks models.Their performance are scrutinized in conjunction with alternative transfer learning approaches like VGG16,Xception,ResNet50,DenseNet121,Inception ResnetV2,and Inception V3.The effectiveness of the proposed custom VGG16 model was gauged by its capacity to generalize to unseen images,yielding an exceptional accuracy of 99.94%,surpassing the benchmarks set by existing state-of-the-art models.Further,the layer wise feature extraction is also visualized as the interpretable AI.
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA28010500)National Natural Science Foundation of China(Grant Nos.42371385,42071420)Zhejiang Provincial Natural Science Foundation of China(Grant No.LTGN23D010002).
文摘As an important rice disease, rice bacterial leaf blight (RBLB, caused by the bacterium Xanthomonas oryzae pv.oryzae), has become widespread in east China in recent years. Significant losses in rice yield occurred as a result ofthe disease’s epidemic, making it imperative to monitor RBLB at a large scale. With the development of remotesensing technology, the broad-band sensors equipped with red-edge channels over multiple spatial resolutionsoffer numerous available data for large-scale monitoring of rice diseases. However, RBLB is characterized by rapiddispersal under suitable conditions, making it difficult to track the disease at a regional scale with a single sensorin practice. Therefore, it is necessary to identify or construct features that are effective across different sensors formonitoring RBLB. To achieve this goal, the spectral response of RBLB was first analyzed based on the canopyhyperspectral data. Using the relative spectral response (RSR) functions of four representative satellite or UAVsensors (i.e., Sentinel-2, GF-6, Planet, and Rededge-M) and the hyperspectral data, the corresponding broad-bandspectral data was simulated. According to a thorough band combination and sensitivity analysis, two novel spectralindices for monitoring RBLB that can be effective across multiple sensors (i.e., RBBRI and RBBDI) weredeveloped. An optimal feature set that includes the two novel indices and a classical vegetation index was formed.The capability of such a feature set in monitoring RBLB was assessed via FLDA and SVM algorithms. The resultdemonstrated that both constructed novel indices exhibited high sensitivity to the disease across multiple sensors.Meanwhile, the feature set yielded an overall accuracy above 90% for all sensors, which indicates its cross-sensorgenerality in monitoring RBLB. The outcome of this research permits disease monitoring with different remotesensing data over a large scale.
文摘Leaf disease recognition using image processing and deep learning techniques is currently a vibrant research area.Most studies have focused on recognizing diseases from images of whole leaves.This approach limits the resulting models’ability to estimate leaf disease severity or identify multiple anomalies occurring on the same leaf.Recent studies have demonstrated that classifying leaf diseases based on individual lesions greatly enhances disease recognition accuracy.In those studies,however,the lesions were laboriously cropped by hand.This study proposes a semi-automatic algorithm that facilitates the fast and efficient preparation of datasets of individual lesions and leaf image pixel maps to overcome this problem.These datasets were then used to train and test lesion classifier and semantic segmentation Convolutional Neural Network(CNN)models,respectively.We report that GoogLeNet’s disease recognition accuracy improved by more than 15%when diseases were recognized from lesion images compared to when disease recognition was done using images of whole leaves.A CNN model which performs semantic segmentation of both the leaf and lesions in one pass is also proposed in this paper.The proposed KijaniNet model achieved state-of-the-art segmentation performance in terms of mean Intersection over Union(mIoU)score of 0.8448 and 0.6257 for the leaf and lesion pixel classes,respectively.In terms of mean boundary F1 score,the KijaniNet model attained 0.8241 and 0.7855 for the two pixel classes,respectively.Lastly,a fully automatic algorithm for leaf disease recognition from individual lesions is proposed.The algorithm employs the semantic segmentation network cascaded to a GoogLeNet classifier for lesion-wise disease recognition.The proposed fully automatic algorithm outperforms competing methods in terms of its superior segmentation and classification performance despite being trained on a small dataset.