The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental sta...The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental stages,the plants produce leaves that curve inward to produce the leafy head.Many microRNAs and their target genes have been described participating in leaf development and leaf curvature.The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage.We compared the mi RNA and gene abundances between emerging rosette and heading leaves.To remove transcripts(miRNAs and genes)whose regulation was most likely associated with plant age rather than the change from rosette to heading stage,we utilized a non-heading collard green(B.oleracea var.acephala)morphotype as control.This resulted in 33 DEMs and 1998 DEGs with likely roles in the transition from rosette to heading stage in cabbage.Among these 1998 DEGs,we found enriched GO terms related to DNA-binding transcription factor activity,transcription regulator activity,iron ion binding,and photosynthesis.We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed(1998DEGs)between rosette and heading stage leaves to construct mi RNA-target gene interaction networks.Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3(GRF3)and 4(GRF4)in pointed cabbage head formation.展开更多
The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was ...The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.展开更多
Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was de...Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.展开更多
基金funded by the Mexican government through the Consejo Nacional de Ciencia y Tecnología (CONACYT),C.V.761325,for the PhD project of Jorge Aleman-Baez。
文摘The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental stages,the plants produce leaves that curve inward to produce the leafy head.Many microRNAs and their target genes have been described participating in leaf development and leaf curvature.The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage.We compared the mi RNA and gene abundances between emerging rosette and heading leaves.To remove transcripts(miRNAs and genes)whose regulation was most likely associated with plant age rather than the change from rosette to heading stage,we utilized a non-heading collard green(B.oleracea var.acephala)morphotype as control.This resulted in 33 DEMs and 1998 DEGs with likely roles in the transition from rosette to heading stage in cabbage.Among these 1998 DEGs,we found enriched GO terms related to DNA-binding transcription factor activity,transcription regulator activity,iron ion binding,and photosynthesis.We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed(1998DEGs)between rosette and heading stage leaves to construct mi RNA-target gene interaction networks.Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3(GRF3)and 4(GRF4)in pointed cabbage head formation.
文摘The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.
文摘Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.
