[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best ...[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.展开更多
Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro ...Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro and in vivo analysis. Methods: The activity of natural killer(NK) cells was measured in splenocytes and YAC-1 cells. Mice were immunosuppressed using cyclophosphamide(5 mg/kg body weight). Three different doses of HCE(200, 400, and 800 mg/kg body weight) and red ginseng extract(800 mg/kg body weight) which was used as standard immunomodulatory herb were administered orally for 4 weeks. The body weight, dietary, water intake, organs(liver, thymus, and spleen) weight, completed blood count, and cytokines(tumor necrosis factor alpha, interferon gamma, and interleukin-2) production was measured. Results: At the maximum concentration of HCE, the activity of NK cells was increased by 48.5%. HCE increased liver, spleen, and thymus weights without altering numbers of white blood cells, lymphocytes, and neutrophils in a cyclophosphamide-induced immunosuppression rat model. However, HCE recovered the inhibited cytokine expression; HCE(800 mg/kg) increased cytokines levels. The results indicate the immune enhancement potential of this HCE. Conclusion: The HCE enhances immunity by increasing NK cell activity, regulating cytokine levels, and maintaining spleen weight. Therefore, it may be used as a potential immunity enhancer.展开更多
基金Supported by 2014 Sichuan Science and Technology Support Plan Program(2014SZ0131)Education Innovation Project of Southwest Minzu University(2015)
文摘[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.
基金Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ01321501) Rural Development Administration,Republic of Korea
文摘Objective: To investigate the effects of a herb complex extract(HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro and in vivo analysis. Methods: The activity of natural killer(NK) cells was measured in splenocytes and YAC-1 cells. Mice were immunosuppressed using cyclophosphamide(5 mg/kg body weight). Three different doses of HCE(200, 400, and 800 mg/kg body weight) and red ginseng extract(800 mg/kg body weight) which was used as standard immunomodulatory herb were administered orally for 4 weeks. The body weight, dietary, water intake, organs(liver, thymus, and spleen) weight, completed blood count, and cytokines(tumor necrosis factor alpha, interferon gamma, and interleukin-2) production was measured. Results: At the maximum concentration of HCE, the activity of NK cells was increased by 48.5%. HCE increased liver, spleen, and thymus weights without altering numbers of white blood cells, lymphocytes, and neutrophils in a cyclophosphamide-induced immunosuppression rat model. However, HCE recovered the inhibited cytokine expression; HCE(800 mg/kg) increased cytokines levels. The results indicate the immune enhancement potential of this HCE. Conclusion: The HCE enhances immunity by increasing NK cell activity, regulating cytokine levels, and maintaining spleen weight. Therefore, it may be used as a potential immunity enhancer.