Since the elucidation that sugar-lectin interactions contribute to the understanding of ‘‘Glycomics' ', how to construct glycosensors with rapid response, excellent sensitivity and selectivity is of intense ...Since the elucidation that sugar-lectin interactions contribute to the understanding of ‘‘Glycomics' ', how to construct glycosensors with rapid response, excellent sensitivity and selectivity is of intense research interest. Herein, we report the design of three NIR emissive glyco-probes based on diketopyrrolopyrrole(DPPs) conjugated with two(DPPG), four(DPPF-G) and six(DPPS-G) galactose groups. All three molecules could probe lectins with excellent sensitivity and selectivity. The increase of glyco-DPP emission in NIR region upon interaction with lectin is due to the aggregates formation induced by sugar-lectin interactions, which have been verified by dynamic light scattering(DLS) and scanning electronic microscope(SEM) analysis.Due to the multiple glyco-ligands on DPPS-G, it has been successfully used to stain Hep G2 cells through interactions between galactose and asialogly-coprotein(ASGP-R), which are overexpressed on the surface of Hep G2 cells.展开更多
目的探索前列腺癌的早期诊断和鉴别诊断的新依据。方法收集前列腺增生和前列腺癌患者空腹第一次晨尿和血标本各90份和30份,随机分为3组,用PSA抗体亲和层析柱提纯尿液和血液中的PSA,用辣根过氧化物酶(HRP)标记的凝集素SNA(sambucus nigra...目的探索前列腺癌的早期诊断和鉴别诊断的新依据。方法收集前列腺增生和前列腺癌患者空腹第一次晨尿和血标本各90份和30份,随机分为3组,用PSA抗体亲和层析柱提纯尿液和血液中的PSA,用辣根过氧化物酶(HRP)标记的凝集素SNA(sambucus nigra agglutinin)做探针,连接到已固定在膜上纯化的PSA的糖链,检测已结合的HRP来代表PSA糖链与凝集素的结合力,用增强化学发光法(ECL)检测已结合的HRP的结合力,其数值用发光单位(LLU)来表示。结果用HRP标记的凝集素SNA-HRP作探针检测前列腺癌和前列腺增生血中PSA时,前列腺癌血中平均LLU仅为前列腺增生血中PSA的18.3%,与SNA-HRP的结合力两者之间有明显差异(P<0.001);而在尿液中两者无明显差异(P>0.05)。说明前列腺癌血中PSA糖链结构中唾液酸残基表达较前列腺增生明显减少。结论前列腺癌患者血中PSA糖链结构与前列腺增生相比发生改变,其中糖链结构中唾液酸残基表达的改变可能为今后前列腺癌的早期诊断和鉴别诊断提供新的依据。展开更多
基金supported by the National Natural Science Foundation of China (21772040, 21421004, 21372082, 21572062)the Fundamental Research Funds for the Central Universities (222201717003)+4 种基金the Programme of Introducing Talents of Discipline to Universities (B16017)a Visiting Program at NUS of China Scholarship Council (CSC)the Singapore Ministry of Education (R279-000-391-112)Singapore NRF Investigatorship (R279-000-444-281)the National University of Singapore (R279-000-482-133)
文摘Since the elucidation that sugar-lectin interactions contribute to the understanding of ‘‘Glycomics' ', how to construct glycosensors with rapid response, excellent sensitivity and selectivity is of intense research interest. Herein, we report the design of three NIR emissive glyco-probes based on diketopyrrolopyrrole(DPPs) conjugated with two(DPPG), four(DPPF-G) and six(DPPS-G) galactose groups. All three molecules could probe lectins with excellent sensitivity and selectivity. The increase of glyco-DPP emission in NIR region upon interaction with lectin is due to the aggregates formation induced by sugar-lectin interactions, which have been verified by dynamic light scattering(DLS) and scanning electronic microscope(SEM) analysis.Due to the multiple glyco-ligands on DPPS-G, it has been successfully used to stain Hep G2 cells through interactions between galactose and asialogly-coprotein(ASGP-R), which are overexpressed on the surface of Hep G2 cells.
文摘目的探索前列腺癌的早期诊断和鉴别诊断的新依据。方法收集前列腺增生和前列腺癌患者空腹第一次晨尿和血标本各90份和30份,随机分为3组,用PSA抗体亲和层析柱提纯尿液和血液中的PSA,用辣根过氧化物酶(HRP)标记的凝集素SNA(sambucus nigra agglutinin)做探针,连接到已固定在膜上纯化的PSA的糖链,检测已结合的HRP来代表PSA糖链与凝集素的结合力,用增强化学发光法(ECL)检测已结合的HRP的结合力,其数值用发光单位(LLU)来表示。结果用HRP标记的凝集素SNA-HRP作探针检测前列腺癌和前列腺增生血中PSA时,前列腺癌血中平均LLU仅为前列腺增生血中PSA的18.3%,与SNA-HRP的结合力两者之间有明显差异(P<0.001);而在尿液中两者无明显差异(P>0.05)。说明前列腺癌血中PSA糖链结构中唾液酸残基表达较前列腺增生明显减少。结论前列腺癌患者血中PSA糖链结构与前列腺增生相比发生改变,其中糖链结构中唾液酸残基表达的改变可能为今后前列腺癌的早期诊断和鉴别诊断提供新的依据。
