目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一...目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一侧髋为实验侧,另一侧作为对照侧,实验侧股骨颈内注射医用TH胶1 m L制作Legg-Perthes病动物模型,对照侧注入生理盐水1 m L。于注胶后4、8、12周时分批处死取材,采用mRNA探针检测Ⅱ型胶原基因型及酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度。结果Ⅱ型胶原基因型及血清Ⅱ型胶原抗体浓度测定发现在第8~12周时实验组与对照组之间均有极显著性差异（P〈0.01）。结论Ⅱ型胶原基因型的改变可能是Legg-perthes病的病因,可能是因为Ⅱ型胶原刺激产生相应抗体发生特异性免疫反应,导致自身免疫攻击,参与Leggperthe病的发病过程。展开更多
Legg-Calvé-Perthes disease(LCPD),a historically-considered osteochondrosis,is now being diagnosed as an idiopathic avascularnecrosis of the femoral head in children.At present,the treatment for LCPD mainly focuse...Legg-Calvé-Perthes disease(LCPD),a historically-considered osteochondrosis,is now being diagnosed as an idiopathic avascularnecrosis of the femoral head in children.At present,the treatment for LCPD mainly focuses on the preservation orrecovery of the arthrogenic component of the femoral head.The currently-available surgical methods are various,most of which can obtain bettertherapeutic effects in LCPD.Nerveless,these surgeries are not universally acknowledged,and may have some limitations like increased articularand vascularinjury.In recent years,Traditional Chinese Medicine(TCM)has been shown a certain advantage in LCPD.This article primarily investigated the key points of common syndromes,TCM nursing methods and healthy guidance of LCPD,aiming at maintaining and developing the strengths of TCM,improving its efficacy and standardizing its behavior.展开更多
文摘目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一侧髋为实验侧,另一侧作为对照侧,实验侧股骨颈内注射医用TH胶1 m L制作Legg-Perthes病动物模型,对照侧注入生理盐水1 m L。于注胶后4、8、12周时分批处死取材,采用mRNA探针检测Ⅱ型胶原基因型及酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度。结果Ⅱ型胶原基因型及血清Ⅱ型胶原抗体浓度测定发现在第8~12周时实验组与对照组之间均有极显著性差异（P〈0.01）。结论Ⅱ型胶原基因型的改变可能是Legg-perthes病的病因,可能是因为Ⅱ型胶原刺激产生相应抗体发生特异性免疫反应,导致自身免疫攻击,参与Leggperthe病的发病过程。
文摘Legg-Calvé-Perthes disease(LCPD),a historically-considered osteochondrosis,is now being diagnosed as an idiopathic avascularnecrosis of the femoral head in children.At present,the treatment for LCPD mainly focuses on the preservation orrecovery of the arthrogenic component of the femoral head.The currently-available surgical methods are various,most of which can obtain bettertherapeutic effects in LCPD.Nerveless,these surgeries are not universally acknowledged,and may have some limitations like increased articularand vascularinjury.In recent years,Traditional Chinese Medicine(TCM)has been shown a certain advantage in LCPD.This article primarily investigated the key points of common syndromes,TCM nursing methods and healthy guidance of LCPD,aiming at maintaining and developing the strengths of TCM,improving its efficacy and standardizing its behavior.
