Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome. SelB binds selenocysteyl-tRNASec, guanine nucleotides and a secondary structure of the selenopr...Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome. SelB binds selenocysteyl-tRNASec, guanine nucleotides and a secondary structure of the selenoprotein mRNA following the UGA at the 3' side. A comparison of the amino acid sequences of SelB species from E. coli,Desulfomicrobium baculatum, Clostridium thermoaceticum and Haemophilus influenzae showed that the proteins consist of at least four structural domains from which the Nterminal three are well conserved and share homology with elongation factor Tu whereas the C-terminal one is more variable and displays no similarity to any protein known. With the aid of the coordinates of EF-Tu the N-terminal part has been modelled into a 3D structure which exhibits intriguing features concerning its interaction with guanine nucleotides and other components of the translational apparatus. Cloning and expression of fragments of SelB and biochemical analysis of the purified truncated proteins showed that the C-terminal 19 kDa protein fragment is able to specifically bind to the selenoprotein mRNA. SelB, thus, is a translation factor functionally homologous to EF-Tu hooked up to the mRNA with its C-terminal end. The formation by SelB of a quaternary complex in vivo has been proven by overexpression of truncated genes of SelB and by demonstration that fragments comprising the mRNA or the tRNA binding domain inhibit selenocysteine insertion展开更多
Mathematical modelling for power DC/DC converters is a historical problem accompanying DC/DC conversion technology since 1940’s. The traditional mathematical modelling is not available for complex structure converter...Mathematical modelling for power DC/DC converters is a historical problem accompanying DC/DC conversion technology since 1940’s. The traditional mathematical modelling is not available for complex structure converters since the differential equation order increases very high. We have to search other way to establish mathematical modelling for power DC/DC converters.We have theoretically defined a new concept-Energy Factor （EF） in this paper and researched the relations between EF and the mathematical modelling for power DC/DC converters. EF is a new concept in power DC/DC conversion technology, which thoroughly differs from the traditional concepts such as power factor （PF）, power transfer efficiency （η）, total harmonic distortion （THD） and ripple factor （RF）. EF and the subsequential EFV (and EFVD) can illustrate the system stability, reference response and interference recovery. This investigation is very helpful for system design and DC/DC converters characteristics foreseeing. Two DC/DC converters: Buck converter and Super-Lift Luo-Converter as the samples are analysed in this paper to demonstrate the applications of EF, EFV (and EFVD), PE, SE, VE （and VED）, time constant τ and damping time constant τd.展开更多
文摘Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome. SelB binds selenocysteyl-tRNASec, guanine nucleotides and a secondary structure of the selenoprotein mRNA following the UGA at the 3' side. A comparison of the amino acid sequences of SelB species from E. coli,Desulfomicrobium baculatum, Clostridium thermoaceticum and Haemophilus influenzae showed that the proteins consist of at least four structural domains from which the Nterminal three are well conserved and share homology with elongation factor Tu whereas the C-terminal one is more variable and displays no similarity to any protein known. With the aid of the coordinates of EF-Tu the N-terminal part has been modelled into a 3D structure which exhibits intriguing features concerning its interaction with guanine nucleotides and other components of the translational apparatus. Cloning and expression of fragments of SelB and biochemical analysis of the purified truncated proteins showed that the C-terminal 19 kDa protein fragment is able to specifically bind to the selenoprotein mRNA. SelB, thus, is a translation factor functionally homologous to EF-Tu hooked up to the mRNA with its C-terminal end. The formation by SelB of a quaternary complex in vivo has been proven by overexpression of truncated genes of SelB and by demonstration that fragments comprising the mRNA or the tRNA binding domain inhibit selenocysteine insertion
文摘Mathematical modelling for power DC/DC converters is a historical problem accompanying DC/DC conversion technology since 1940’s. The traditional mathematical modelling is not available for complex structure converters since the differential equation order increases very high. We have to search other way to establish mathematical modelling for power DC/DC converters.We have theoretically defined a new concept-Energy Factor （EF） in this paper and researched the relations between EF and the mathematical modelling for power DC/DC converters. EF is a new concept in power DC/DC conversion technology, which thoroughly differs from the traditional concepts such as power factor （PF）, power transfer efficiency （η）, total harmonic distortion （THD） and ripple factor （RF）. EF and the subsequential EFV (and EFVD) can illustrate the system stability, reference response and interference recovery. This investigation is very helpful for system design and DC/DC converters characteristics foreseeing. Two DC/DC converters: Buck converter and Super-Lift Luo-Converter as the samples are analysed in this paper to demonstrate the applications of EF, EFV (and EFVD), PE, SE, VE （and VED）, time constant τ and damping time constant τd.
